New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina

Autores
Moya, Paulina; Barrera, Viviana Andrea; Cipollone, Josefina; Bedoya, Carolina; Kohan, Lucila; Toledo, Andrea; Sisterna, Marina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The fungus Pyrenophora teres, causal agent of net blotch of barley, generates important yield losses, mainly regarding grain weight and quality of malt extract for beer production. In order to find new strategies to manage this disease, we evaluated in vitro and in vivo antagonistic interactions among different native isolates of Trichoderma spp. and P. teres. The microorganisms were isolated from different barley crop areas of Buenos Aires province and identified according to morphological features and molecular techniques. Dual cultures were conducted to assess in vitro antagonism using direct interactions. Assays under greenhouse conditions were carried out to evaluate the efficiency of Trichoderma spp. as biocontrol and plant growth–promoting agent. In vitro, all Trichoderma spp. isolates inhibited mycelial growth of P. teres in an 18%–54% range compared to the controls. Microscopic observations of interaction zones revealed morphological alterations of P. teres such as vacuolated mycelium without typical pigmentation and coiling. In vivo, all Trichoderma isolates significantly decreased the incidence of P. teres, up to 55% on barley seedlings. Severity decreased up to 77% on stems and up to 70% on leaves. Likewise, regarding growth–promoting parameters, aerial and radicular dry weight of plants treated with Trichoderma spp. increased up to 20% and 15%, respectively. On the other hand, an increase of chlorophyll (SPAD) of up to 9% compared to the control was recorded in seedlings treated with Trichoderma.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Moya, Paulina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Moya, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Cipollone, Josefina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Cipollone, Josefina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA); Argentina
Fil: Bedoya, Carolina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Kohan, Lucila. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Toledo, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Toledo, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Sisterna, Marina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Sisterna, Marina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA); Argentina
Fuente
Biological Control 141 : 104152 (Febrero 2020)
Materia
Hongos
Fungi
Barley
Trichoderma
Pyrenophora teres
Cebada
Hypocreales
Antagonistic Effect
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16612
Acceder
id INTADig_0ff10a9a9bfe8c4c3368d17134bda33f
oai_identifier_str oai:localhost:20.500.12123/16612
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in ArgentinaMoya, PaulinaBarrera, Viviana AndreaCipollone, JosefinaBedoya, CarolinaKohan, LucilaToledo, AndreaSisterna, MarinaHongosFungiBarleyTrichodermaPyrenophora teresCebadaHypocrealesAntagonistic EffectThe fungus Pyrenophora teres, causal agent of net blotch of barley, generates important yield losses, mainly regarding grain weight and quality of malt extract for beer production. In order to find new strategies to manage this disease, we evaluated in vitro and in vivo antagonistic interactions among different native isolates of Trichoderma spp. and P. teres. The microorganisms were isolated from different barley crop areas of Buenos Aires province and identified according to morphological features and molecular techniques. Dual cultures were conducted to assess in vitro antagonism using direct interactions. Assays under greenhouse conditions were carried out to evaluate the efficiency of Trichoderma spp. as biocontrol and plant growth–promoting agent. In vitro, all Trichoderma spp. isolates inhibited mycelial growth of P. teres in an 18%–54% range compared to the controls. Microscopic observations of interaction zones revealed morphological alterations of P. teres such as vacuolated mycelium without typical pigmentation and coiling. In vivo, all Trichoderma isolates significantly decreased the incidence of P. teres, up to 55% on barley seedlings. Severity decreased up to 77% on stems and up to 70% on leaves. Likewise, regarding growth–promoting parameters, aerial and radicular dry weight of plants treated with Trichoderma spp. increased up to 20% and 15%, respectively. On the other hand, an increase of chlorophyll (SPAD) of up to 9% compared to the control was recorded in seedlings treated with Trichoderma.Instituto de Microbiología y Zoología Agrícola (IMYZA)Fil: Moya, Paulina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); ArgentinaFil: Moya, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; ArgentinaFil: Cipollone, Josefina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); ArgentinaFil: Cipollone, Josefina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA); ArgentinaFil: Bedoya, Carolina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); ArgentinaFil: Kohan, Lucila. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); ArgentinaFil: Toledo, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); ArgentinaFil: Toledo, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Sisterna, Marina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); ArgentinaFil: Sisterna, Marina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA); ArgentinaElsevier2024-02-15T11:11:18Z2024-02-15T11:11:18Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16612https://www.sciencedirect.com/science/article/pii/S10499644193048271090-21121049-9644https://doi.org/10.1016/j.biocontrol.2019.104152Biological Control 141 : 104152 (Febrero 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:24:56Zoai:localhost:20.500.12123/16612instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:24:56.904INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
title New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
spellingShingle New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
Moya, Paulina
Hongos
Fungi
Barley
Trichoderma
Pyrenophora teres
Cebada
Hypocreales
Antagonistic Effect
title_short New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
title_full New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
title_fullStr New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
title_full_unstemmed New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
title_sort New isolates of Trichoderma spp. as biocontrol and plant growth–promoting agents in the pathosystem Pyrenophora teres-barley in Argentina
dc.creator.none.fl_str_mv Moya, Paulina
Barrera, Viviana Andrea
Cipollone, Josefina
Bedoya, Carolina
Kohan, Lucila
Toledo, Andrea
Sisterna, Marina
author Moya, Paulina
author_facet Moya, Paulina
Barrera, Viviana Andrea
Cipollone, Josefina
Bedoya, Carolina
Kohan, Lucila
Toledo, Andrea
Sisterna, Marina
author_role author
author2 Barrera, Viviana Andrea
Cipollone, Josefina
Bedoya, Carolina
Kohan, Lucila
Toledo, Andrea
Sisterna, Marina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hongos
Fungi
Barley
Trichoderma
Pyrenophora teres
Cebada
Hypocreales
Antagonistic Effect
topic Hongos
Fungi
Barley
Trichoderma
Pyrenophora teres
Cebada
Hypocreales
Antagonistic Effect
dc.description.none.fl_txt_mv The fungus Pyrenophora teres, causal agent of net blotch of barley, generates important yield losses, mainly regarding grain weight and quality of malt extract for beer production. In order to find new strategies to manage this disease, we evaluated in vitro and in vivo antagonistic interactions among different native isolates of Trichoderma spp. and P. teres. The microorganisms were isolated from different barley crop areas of Buenos Aires province and identified according to morphological features and molecular techniques. Dual cultures were conducted to assess in vitro antagonism using direct interactions. Assays under greenhouse conditions were carried out to evaluate the efficiency of Trichoderma spp. as biocontrol and plant growth–promoting agent. In vitro, all Trichoderma spp. isolates inhibited mycelial growth of P. teres in an 18%–54% range compared to the controls. Microscopic observations of interaction zones revealed morphological alterations of P. teres such as vacuolated mycelium without typical pigmentation and coiling. In vivo, all Trichoderma isolates significantly decreased the incidence of P. teres, up to 55% on barley seedlings. Severity decreased up to 77% on stems and up to 70% on leaves. Likewise, regarding growth–promoting parameters, aerial and radicular dry weight of plants treated with Trichoderma spp. increased up to 20% and 15%, respectively. On the other hand, an increase of chlorophyll (SPAD) of up to 9% compared to the control was recorded in seedlings treated with Trichoderma.
Instituto de Microbiología y Zoología Agrícola (IMYZA)
Fil: Moya, Paulina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Moya, Paulina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina
Fil: Cipollone, Josefina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Cipollone, Josefina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA); Argentina
Fil: Bedoya, Carolina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Kohan, Lucila. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Toledo, Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Toledo, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Sisterna, Marina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Centro de Investigaciones de Fitopatología (CIDEFI); Argentina
Fil: Sisterna, Marina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CICPBA); Argentina
description The fungus Pyrenophora teres, causal agent of net blotch of barley, generates important yield losses, mainly regarding grain weight and quality of malt extract for beer production. In order to find new strategies to manage this disease, we evaluated in vitro and in vivo antagonistic interactions among different native isolates of Trichoderma spp. and P. teres. The microorganisms were isolated from different barley crop areas of Buenos Aires province and identified according to morphological features and molecular techniques. Dual cultures were conducted to assess in vitro antagonism using direct interactions. Assays under greenhouse conditions were carried out to evaluate the efficiency of Trichoderma spp. as biocontrol and plant growth–promoting agent. In vitro, all Trichoderma spp. isolates inhibited mycelial growth of P. teres in an 18%–54% range compared to the controls. Microscopic observations of interaction zones revealed morphological alterations of P. teres such as vacuolated mycelium without typical pigmentation and coiling. In vivo, all Trichoderma isolates significantly decreased the incidence of P. teres, up to 55% on barley seedlings. Severity decreased up to 77% on stems and up to 70% on leaves. Likewise, regarding growth–promoting parameters, aerial and radicular dry weight of plants treated with Trichoderma spp. increased up to 20% and 15%, respectively. On the other hand, an increase of chlorophyll (SPAD) of up to 9% compared to the control was recorded in seedlings treated with Trichoderma.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
2024-02-15T11:11:18Z
2024-02-15T11:11:18Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16612
https://www.sciencedirect.com/science/article/pii/S1049964419304827
1090-2112
1049-9644
https://doi.org/10.1016/j.biocontrol.2019.104152
url http://hdl.handle.net/20.500.12123/16612
https://www.sciencedirect.com/science/article/pii/S1049964419304827
https://doi.org/10.1016/j.biocontrol.2019.104152
identifier_str_mv 1090-2112
1049-9644
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Biological Control 141 : 104152 (Febrero 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842975524137205760
score 12.993085

Publicaciones similares