Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch

Autores
Moya, Paulina; Girotti, Juan Roberto; Toledo, Andrea Vanesa; Sisterna, Marina Noemí
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Many species of Trichoderma produce secondary metabolites such as volatile organic compounds (VOCs) that reduce plant diseases and promote their growth. In this work we evaluated the antagonistic effects of VOCs released by eight strains of two Trichoderma species against Pyrenophora teres Drechsler, the causal agent of barley net blotch. Antagonism was estimated based on the percentage of mycelial growth inhibition according to the confronted cultures method. VOCs extraction and identification were performed by gas chromatography and mass spectrometry, through different methodologies for VOCs emitted by antagonists and pathogens alone or when confronted. VOCs produced by all Trichoderma strains inhibited mycelial growth of the pathogen in a range of 3 to 32%, showing weak and unpigmented mycelia with vacuolization. In addition, P. teres stimulated the release of VOCs by both Trichoderma species. The major groups of VOCs detected were sesquiterpenes, followed by diterpenes, terpenoids and eight-carbon compounds. This is the first report about characterization of volatiles emitted by Trichoderma in the presence of P. teres.
Fil: Moya, Paulina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Girotti, Juan Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Toledo, Andrea Vanesa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sisterna, Marina Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
Materia
BIOCONTROL
BIOPESTICIDES
FUNGI
GAS CHROMATOGRAPHY
MASS SPECTROMETRY
PATHOGENS
PYRENOPHORA TERES
TRICHODERMA
VOCS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/96931
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/96931
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotchMoya, PaulinaGirotti, Juan RobertoToledo, Andrea VanesaSisterna, Marina NoemíBIOCONTROLBIOPESTICIDESFUNGIGAS CHROMATOGRAPHYMASS SPECTROMETRYPATHOGENSPYRENOPHORA TERESTRICHODERMAVOCShttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Many species of Trichoderma produce secondary metabolites such as volatile organic compounds (VOCs) that reduce plant diseases and promote their growth. In this work we evaluated the antagonistic effects of VOCs released by eight strains of two Trichoderma species against Pyrenophora teres Drechsler, the causal agent of barley net blotch. Antagonism was estimated based on the percentage of mycelial growth inhibition according to the confronted cultures method. VOCs extraction and identification were performed by gas chromatography and mass spectrometry, through different methodologies for VOCs emitted by antagonists and pathogens alone or when confronted. VOCs produced by all Trichoderma strains inhibited mycelial growth of the pathogen in a range of 3 to 32%, showing weak and unpigmented mycelia with vacuolization. In addition, P. teres stimulated the release of VOCs by both Trichoderma species. The major groups of VOCs detected were sesquiterpenes, followed by diterpenes, terpenoids and eight-carbon compounds. This is the first report about characterization of volatiles emitted by Trichoderma in the presence of P. teres.Fil: Moya, Paulina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Girotti, Juan Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; ArgentinaFil: Toledo, Andrea Vanesa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sisterna, Marina Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; ArgentinaPolish Academy of Sciences2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/96931Moya, Paulina; Girotti, Juan Roberto; Toledo, Andrea Vanesa; Sisterna, Marina Noemí; Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch; Polish Academy of Sciences; Journal of Plant Protection Research; 58; 1; 1-2018; 45-531427-4345CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.24425/119115info:eu-repo/semantics/altIdentifier/url/http://www.plantprotection.pl/Antifungal-activity-of-Trichoderma-VOCs-against-Pyrenophora-teres-the-causal-agent,80929,0,2.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:50Zoai:ri.conicet.gov.ar:11336/96931instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:50.724CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
title Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
spellingShingle Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
Moya, Paulina
BIOCONTROL
BIOPESTICIDES
FUNGI
GAS CHROMATOGRAPHY
MASS SPECTROMETRY
PATHOGENS
PYRENOPHORA TERES
TRICHODERMA
VOCS
title_short Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
title_full Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
title_fullStr Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
title_full_unstemmed Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
title_sort Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch
dc.creator.none.fl_str_mv Moya, Paulina
Girotti, Juan Roberto
Toledo, Andrea Vanesa
Sisterna, Marina Noemí
author Moya, Paulina
author_facet Moya, Paulina
Girotti, Juan Roberto
Toledo, Andrea Vanesa
Sisterna, Marina Noemí
author_role author
author2 Girotti, Juan Roberto
Toledo, Andrea Vanesa
Sisterna, Marina Noemí
author2_role author
author
author
dc.subject.none.fl_str_mv BIOCONTROL
BIOPESTICIDES
FUNGI
GAS CHROMATOGRAPHY
MASS SPECTROMETRY
PATHOGENS
PYRENOPHORA TERES
TRICHODERMA
VOCS
topic BIOCONTROL
BIOPESTICIDES
FUNGI
GAS CHROMATOGRAPHY
MASS SPECTROMETRY
PATHOGENS
PYRENOPHORA TERES
TRICHODERMA
VOCS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Many species of Trichoderma produce secondary metabolites such as volatile organic compounds (VOCs) that reduce plant diseases and promote their growth. In this work we evaluated the antagonistic effects of VOCs released by eight strains of two Trichoderma species against Pyrenophora teres Drechsler, the causal agent of barley net blotch. Antagonism was estimated based on the percentage of mycelial growth inhibition according to the confronted cultures method. VOCs extraction and identification were performed by gas chromatography and mass spectrometry, through different methodologies for VOCs emitted by antagonists and pathogens alone or when confronted. VOCs produced by all Trichoderma strains inhibited mycelial growth of the pathogen in a range of 3 to 32%, showing weak and unpigmented mycelia with vacuolization. In addition, P. teres stimulated the release of VOCs by both Trichoderma species. The major groups of VOCs detected were sesquiterpenes, followed by diterpenes, terpenoids and eight-carbon compounds. This is the first report about characterization of volatiles emitted by Trichoderma in the presence of P. teres.
Fil: Moya, Paulina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Girotti, Juan Roberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina
Fil: Toledo, Andrea Vanesa. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sisterna, Marina Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales. Departamento de Ciencias Biológicas. Centro de Investigaciones de Fitopatología. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones de Fitopatología; Argentina
description Many species of Trichoderma produce secondary metabolites such as volatile organic compounds (VOCs) that reduce plant diseases and promote their growth. In this work we evaluated the antagonistic effects of VOCs released by eight strains of two Trichoderma species against Pyrenophora teres Drechsler, the causal agent of barley net blotch. Antagonism was estimated based on the percentage of mycelial growth inhibition according to the confronted cultures method. VOCs extraction and identification were performed by gas chromatography and mass spectrometry, through different methodologies for VOCs emitted by antagonists and pathogens alone or when confronted. VOCs produced by all Trichoderma strains inhibited mycelial growth of the pathogen in a range of 3 to 32%, showing weak and unpigmented mycelia with vacuolization. In addition, P. teres stimulated the release of VOCs by both Trichoderma species. The major groups of VOCs detected were sesquiterpenes, followed by diterpenes, terpenoids and eight-carbon compounds. This is the first report about characterization of volatiles emitted by Trichoderma in the presence of P. teres.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/96931
Moya, Paulina; Girotti, Juan Roberto; Toledo, Andrea Vanesa; Sisterna, Marina Noemí; Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch; Polish Academy of Sciences; Journal of Plant Protection Research; 58; 1; 1-2018; 45-53
1427-4345
CONICET Digital
CONICET
url http://hdl.handle.net/11336/96931
identifier_str_mv Moya, Paulina; Girotti, Juan Roberto; Toledo, Andrea Vanesa; Sisterna, Marina Noemí; Antifungal activity of Trichoderma VOCs against Pyrenophora teres, the causal agent of barley net blotch; Polish Academy of Sciences; Journal of Plant Protection Research; 58; 1; 1-2018; 45-53
1427-4345
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.24425/119115
info:eu-repo/semantics/altIdentifier/url/http://www.plantprotection.pl/Antifungal-activity-of-Trichoderma-VOCs-against-Pyrenophora-teres-the-causal-agent,80929,0,2.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Polish Academy of Sciences
publisher.none.fl_str_mv Polish Academy of Sciences
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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