Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses

Autores
Basso, Walter; Holenweger, Fabienne; Schares, Gereon; Müller, Norbert; Campero, Lucia Maria; Ardüser, Flurin; Moore-Jones, Gaia; Frey, Caroline; Zanolari, Patrik
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Toxoplasma gondii and Neospora caninum infections are important causes of abortion in ruminants. Besides, meat from T. gondii infected animals represent a major infection source for humans. The occurrence of these protozoan parasites in Switzerland was investigated both, in a nationwide cross-sectional serological survey, and by molecular methods in aborted sheep and goat foetuses. A total of 653 sheep from 143 farms and 748 goats from 164 farms were tested by commercial ELISAs and inconclusive results were defined by immunoblot. Besides, a risk factor analysis for seropositivity was performed. The observed seroprevalences for T. gondii in sheep and goats were 66.3% and 50.5% at the animal level, and 90.9% and 81.1% at the farm level, respectively. For N. caninum, the detected seroprevalences in sheep and goats were 0.8% and 0.9% at the animal level, and 2.8% and 1.8% at the farm level, respectively. Older small ruminants, and sheep (vs. goats) had a higher risk of being seropositive to T. gondii. Alpine grazing in summer was identified as a protective factor for seropositivity to T. gondii in both animal species. Toxoplasma gondii and N. caninum DNA were detected in 6.1% and 2.4% (n = 82), and in 6.8% and 1.4% (n = 73) of the tested ovine and caprine foetuses, respectively. These results suggest the involvement of these parasites in abortions and reveal a high prevalence of T. gondii and lower prevalence of N. caninum infections in small ruminants in Switzerland. They also suggest that consumption of undercooked meat from T. gondii infected sheep and goats may represent a risk for public health.
EEA Balcarce
Fil: Basso, Walter. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Holenweger, Fabienne. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Schares, Gereon. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania.
Fil: Müller, Norbert. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Campero, Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Campero, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Ardüser, Flurin. University of Bern. Vetsuisse-Faculty. Clinic for Ruminants; Suiza.
Fil: Moore-Jones, Gaia. University of Bern. Vetsuisse-Faculty. Institute for Fish and Wildlife Health; Suiza.
Fil: Frey, Caroline. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Zanolari, Patrik. University of Bern. Vetsuisse-Faculty. Clinic for Ruminants; Suiza.
Fuente
Food and Waterborne Parasitology 28 : e00176. (September 2022)
Materia
Toxoplasma Gondii
Neospora caninum
Aborto
Serología
Zoonosis
Abortion
Serology
PCR
Sheep
Goats
Zoonoses
Ovinos
Caprinos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/15194
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network_name_str INTA Digital (INTA)
spelling Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetusesBasso, WalterHolenweger, FabienneSchares, GereonMüller, NorbertCampero, Lucia MariaArdüser, FlurinMoore-Jones, GaiaFrey, CarolineZanolari, PatrikToxoplasma GondiiNeospora caninumAbortoSerologíaZoonosisAbortionSerologyPCRSheepGoatsZoonosesOvinosCaprinosToxoplasma gondii and Neospora caninum infections are important causes of abortion in ruminants. Besides, meat from T. gondii infected animals represent a major infection source for humans. The occurrence of these protozoan parasites in Switzerland was investigated both, in a nationwide cross-sectional serological survey, and by molecular methods in aborted sheep and goat foetuses. A total of 653 sheep from 143 farms and 748 goats from 164 farms were tested by commercial ELISAs and inconclusive results were defined by immunoblot. Besides, a risk factor analysis for seropositivity was performed. The observed seroprevalences for T. gondii in sheep and goats were 66.3% and 50.5% at the animal level, and 90.9% and 81.1% at the farm level, respectively. For N. caninum, the detected seroprevalences in sheep and goats were 0.8% and 0.9% at the animal level, and 2.8% and 1.8% at the farm level, respectively. Older small ruminants, and sheep (vs. goats) had a higher risk of being seropositive to T. gondii. Alpine grazing in summer was identified as a protective factor for seropositivity to T. gondii in both animal species. Toxoplasma gondii and N. caninum DNA were detected in 6.1% and 2.4% (n = 82), and in 6.8% and 1.4% (n = 73) of the tested ovine and caprine foetuses, respectively. These results suggest the involvement of these parasites in abortions and reveal a high prevalence of T. gondii and lower prevalence of N. caninum infections in small ruminants in Switzerland. They also suggest that consumption of undercooked meat from T. gondii infected sheep and goats may represent a risk for public health.EEA BalcarceFil: Basso, Walter. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.Fil: Holenweger, Fabienne. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.Fil: Schares, Gereon. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania.Fil: Müller, Norbert. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.Fil: Campero, Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.Fil: Campero, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.Fil: Ardüser, Flurin. University of Bern. Vetsuisse-Faculty. Clinic for Ruminants; Suiza.Fil: Moore-Jones, Gaia. University of Bern. Vetsuisse-Faculty. Institute for Fish and Wildlife Health; Suiza.Fil: Frey, Caroline. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.Fil: Zanolari, Patrik. University of Bern. Vetsuisse-Faculty. Clinic for Ruminants; Suiza.Elsevier2023-09-13T11:14:53Z2023-09-13T11:14:53Z2022-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15194https://www.sciencedirect.com/science/article/pii/S24056766220003362405-6766https://doi.org/10.1016/j.fawpar.2022.e00176Food and Waterborne Parasitology 28 : e00176. (September 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:56Zoai:localhost:20.500.12123/15194instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:56.488INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses
title Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses
spellingShingle Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses
Basso, Walter
Toxoplasma Gondii
Neospora caninum
Aborto
Serología
Zoonosis
Abortion
Serology
PCR
Sheep
Goats
Zoonoses
Ovinos
Caprinos
title_short Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses
title_full Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses
title_fullStr Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses
title_full_unstemmed Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses
title_sort Toxoplasma gondii and Neospora caninum infections in sheep and goats in Switzerland: Seroprevalence and occurrence in aborted fetuses
dc.creator.none.fl_str_mv Basso, Walter
Holenweger, Fabienne
Schares, Gereon
Müller, Norbert
Campero, Lucia Maria
Ardüser, Flurin
Moore-Jones, Gaia
Frey, Caroline
Zanolari, Patrik
author Basso, Walter
author_facet Basso, Walter
Holenweger, Fabienne
Schares, Gereon
Müller, Norbert
Campero, Lucia Maria
Ardüser, Flurin
Moore-Jones, Gaia
Frey, Caroline
Zanolari, Patrik
author_role author
author2 Holenweger, Fabienne
Schares, Gereon
Müller, Norbert
Campero, Lucia Maria
Ardüser, Flurin
Moore-Jones, Gaia
Frey, Caroline
Zanolari, Patrik
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Toxoplasma Gondii
Neospora caninum
Aborto
Serología
Zoonosis
Abortion
Serology
PCR
Sheep
Goats
Zoonoses
Ovinos
Caprinos
topic Toxoplasma Gondii
Neospora caninum
Aborto
Serología
Zoonosis
Abortion
Serology
PCR
Sheep
Goats
Zoonoses
Ovinos
Caprinos
dc.description.none.fl_txt_mv Toxoplasma gondii and Neospora caninum infections are important causes of abortion in ruminants. Besides, meat from T. gondii infected animals represent a major infection source for humans. The occurrence of these protozoan parasites in Switzerland was investigated both, in a nationwide cross-sectional serological survey, and by molecular methods in aborted sheep and goat foetuses. A total of 653 sheep from 143 farms and 748 goats from 164 farms were tested by commercial ELISAs and inconclusive results were defined by immunoblot. Besides, a risk factor analysis for seropositivity was performed. The observed seroprevalences for T. gondii in sheep and goats were 66.3% and 50.5% at the animal level, and 90.9% and 81.1% at the farm level, respectively. For N. caninum, the detected seroprevalences in sheep and goats were 0.8% and 0.9% at the animal level, and 2.8% and 1.8% at the farm level, respectively. Older small ruminants, and sheep (vs. goats) had a higher risk of being seropositive to T. gondii. Alpine grazing in summer was identified as a protective factor for seropositivity to T. gondii in both animal species. Toxoplasma gondii and N. caninum DNA were detected in 6.1% and 2.4% (n = 82), and in 6.8% and 1.4% (n = 73) of the tested ovine and caprine foetuses, respectively. These results suggest the involvement of these parasites in abortions and reveal a high prevalence of T. gondii and lower prevalence of N. caninum infections in small ruminants in Switzerland. They also suggest that consumption of undercooked meat from T. gondii infected sheep and goats may represent a risk for public health.
EEA Balcarce
Fil: Basso, Walter. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Holenweger, Fabienne. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Schares, Gereon. Institute of Epidemiology. Federal Research Institute for Animal Health. Friedrich-Loeffler-Institut; Alemania.
Fil: Müller, Norbert. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Campero, Lucía. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Campero, Lucía. Universidad Nacional de La Plata. Facultad de Ciencias Vetrinarias; Argentina.
Fil: Ardüser, Flurin. University of Bern. Vetsuisse-Faculty. Clinic for Ruminants; Suiza.
Fil: Moore-Jones, Gaia. University of Bern. Vetsuisse-Faculty. Institute for Fish and Wildlife Health; Suiza.
Fil: Frey, Caroline. University of Bern. Vetsuisse-Faculty. Institute of Parasitology; Suiza.
Fil: Zanolari, Patrik. University of Bern. Vetsuisse-Faculty. Clinic for Ruminants; Suiza.
description Toxoplasma gondii and Neospora caninum infections are important causes of abortion in ruminants. Besides, meat from T. gondii infected animals represent a major infection source for humans. The occurrence of these protozoan parasites in Switzerland was investigated both, in a nationwide cross-sectional serological survey, and by molecular methods in aborted sheep and goat foetuses. A total of 653 sheep from 143 farms and 748 goats from 164 farms were tested by commercial ELISAs and inconclusive results were defined by immunoblot. Besides, a risk factor analysis for seropositivity was performed. The observed seroprevalences for T. gondii in sheep and goats were 66.3% and 50.5% at the animal level, and 90.9% and 81.1% at the farm level, respectively. For N. caninum, the detected seroprevalences in sheep and goats were 0.8% and 0.9% at the animal level, and 2.8% and 1.8% at the farm level, respectively. Older small ruminants, and sheep (vs. goats) had a higher risk of being seropositive to T. gondii. Alpine grazing in summer was identified as a protective factor for seropositivity to T. gondii in both animal species. Toxoplasma gondii and N. caninum DNA were detected in 6.1% and 2.4% (n = 82), and in 6.8% and 1.4% (n = 73) of the tested ovine and caprine foetuses, respectively. These results suggest the involvement of these parasites in abortions and reveal a high prevalence of T. gondii and lower prevalence of N. caninum infections in small ruminants in Switzerland. They also suggest that consumption of undercooked meat from T. gondii infected sheep and goats may represent a risk for public health.
publishDate 2022
dc.date.none.fl_str_mv 2022-09
2023-09-13T11:14:53Z
2023-09-13T11:14:53Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15194
https://www.sciencedirect.com/science/article/pii/S2405676622000336
2405-6766
https://doi.org/10.1016/j.fawpar.2022.e00176
url http://hdl.handle.net/20.500.12123/15194
https://www.sciencedirect.com/science/article/pii/S2405676622000336
https://doi.org/10.1016/j.fawpar.2022.e00176
identifier_str_mv 2405-6766
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Food and Waterborne Parasitology 28 : e00176. (September 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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