Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements
- Autores
- Cambiagno, Damián Alejandro; Nota, María Florencia; Zavallo, Diego; Rius, Sebastián Pablo; Casati, Paula; Alvarez, Maria Elena; Asurmendi, Sebastian
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Pattern recognition receptors (PRR) and nucleotide‐binding leucine‐rich repeat proteins (NLR) are major components of the plant immune system responsible for pathogen detection. To date, the transcriptional regulation of PRR/NLR genes is poorly understood. Some PRR/NLR genes are affected by epigenetic changes of neighboring transposable elements (TEs) (cis regulation). We analyzed whether these genes can also respond to changes in the epigenetic marks of distal pericentromeric TEs (trans regulation). We found that Arabidopsis tissues infected with Pseudomonas syringae pv. tomato (Pst) initially induced the expression of pericentromeric TEs, and then repressed it by RNA‐directed DNA methylation (RdDM). The latter response was accompanied by the accumulation of small RNAs (sRNAs) mapping to the TEs. Curiously these sRNAs also mapped to distal PRR/NLR genes, which were controlled by RdDM but remained induced in the infected tissues. Then, we used non‐infected mom1 (Morpheus’ molecule 1) mutants that expressed pericentromeric TEs to test if they lose repression of PRR/NLR genes. mom1 plants activated several PRR/NLR genes that were unlinked to MOM1‐targeted TEs, and showed enhanced resistance to Pst. Remarkably, the increased defenses of mom1 were abolished when MOM1/RdDM‐mediated pericentromeric TEs silencing was re‐established. Therefore, common sRNAs could control PRR/NLR genes and distal pericentromeric TEs and preferentially silence TEs when they are activated.
Instituto de Biotecnología
Fil: Cambiagno, Damián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Nota, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Rius, Sebastián Pablo. Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Casati, Paula. Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Alvarez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina - Fuente
- Plant journal 96 (6) : 1178-1190. (December 2018)
- Materia
-
Arabidopsis Thaliana
Transposones
Epigenético
Transposons
Epigenetics
Defense Mechanisms
RdDM
MOM1 - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4488
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Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elementsCambiagno, Damián AlejandroNota, María FlorenciaZavallo, DiegoRius, Sebastián PabloCasati, PaulaAlvarez, Maria ElenaAsurmendi, SebastianArabidopsis ThalianaTransposonesEpigenéticoTransposonsEpigeneticsDefense MechanismsRdDMMOM1Pattern recognition receptors (PRR) and nucleotide‐binding leucine‐rich repeat proteins (NLR) are major components of the plant immune system responsible for pathogen detection. To date, the transcriptional regulation of PRR/NLR genes is poorly understood. Some PRR/NLR genes are affected by epigenetic changes of neighboring transposable elements (TEs) (cis regulation). We analyzed whether these genes can also respond to changes in the epigenetic marks of distal pericentromeric TEs (trans regulation). We found that Arabidopsis tissues infected with Pseudomonas syringae pv. tomato (Pst) initially induced the expression of pericentromeric TEs, and then repressed it by RNA‐directed DNA methylation (RdDM). The latter response was accompanied by the accumulation of small RNAs (sRNAs) mapping to the TEs. Curiously these sRNAs also mapped to distal PRR/NLR genes, which were controlled by RdDM but remained induced in the infected tissues. Then, we used non‐infected mom1 (Morpheus’ molecule 1) mutants that expressed pericentromeric TEs to test if they lose repression of PRR/NLR genes. mom1 plants activated several PRR/NLR genes that were unlinked to MOM1‐targeted TEs, and showed enhanced resistance to Pst. Remarkably, the increased defenses of mom1 were abolished when MOM1/RdDM‐mediated pericentromeric TEs silencing was re‐established. Therefore, common sRNAs could control PRR/NLR genes and distal pericentromeric TEs and preferentially silence TEs when they are activated.Instituto de BiotecnologíaFil: Cambiagno, Damián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Nota, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Rius, Sebastián Pablo. Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Casati, Paula. Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Alvarez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; ArgentinaWiley2019-02-25T17:26:22Z2019-02-25T17:26:22Z2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/44881365-313Xhttps://doi.org/10.1111/tpj.14098Plant journal 96 (6) : 1178-1190. (December 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:26Zoai:localhost:20.500.12123/4488instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:27.361INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title |
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| spellingShingle |
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements Cambiagno, Damián Alejandro Arabidopsis Thaliana Transposones Epigenético Transposons Epigenetics Defense Mechanisms RdDM MOM1 |
| title_short |
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_full |
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_fullStr |
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_full_unstemmed |
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| title_sort |
Immune receptor genes and pericentromeric transposons as targets of common epigenetic regulatory elements |
| dc.creator.none.fl_str_mv |
Cambiagno, Damián Alejandro Nota, María Florencia Zavallo, Diego Rius, Sebastián Pablo Casati, Paula Alvarez, Maria Elena Asurmendi, Sebastian |
| author |
Cambiagno, Damián Alejandro |
| author_facet |
Cambiagno, Damián Alejandro Nota, María Florencia Zavallo, Diego Rius, Sebastián Pablo Casati, Paula Alvarez, Maria Elena Asurmendi, Sebastian |
| author_role |
author |
| author2 |
Nota, María Florencia Zavallo, Diego Rius, Sebastián Pablo Casati, Paula Alvarez, Maria Elena Asurmendi, Sebastian |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Arabidopsis Thaliana Transposones Epigenético Transposons Epigenetics Defense Mechanisms RdDM MOM1 |
| topic |
Arabidopsis Thaliana Transposones Epigenético Transposons Epigenetics Defense Mechanisms RdDM MOM1 |
| dc.description.none.fl_txt_mv |
Pattern recognition receptors (PRR) and nucleotide‐binding leucine‐rich repeat proteins (NLR) are major components of the plant immune system responsible for pathogen detection. To date, the transcriptional regulation of PRR/NLR genes is poorly understood. Some PRR/NLR genes are affected by epigenetic changes of neighboring transposable elements (TEs) (cis regulation). We analyzed whether these genes can also respond to changes in the epigenetic marks of distal pericentromeric TEs (trans regulation). We found that Arabidopsis tissues infected with Pseudomonas syringae pv. tomato (Pst) initially induced the expression of pericentromeric TEs, and then repressed it by RNA‐directed DNA methylation (RdDM). The latter response was accompanied by the accumulation of small RNAs (sRNAs) mapping to the TEs. Curiously these sRNAs also mapped to distal PRR/NLR genes, which were controlled by RdDM but remained induced in the infected tissues. Then, we used non‐infected mom1 (Morpheus’ molecule 1) mutants that expressed pericentromeric TEs to test if they lose repression of PRR/NLR genes. mom1 plants activated several PRR/NLR genes that were unlinked to MOM1‐targeted TEs, and showed enhanced resistance to Pst. Remarkably, the increased defenses of mom1 were abolished when MOM1/RdDM‐mediated pericentromeric TEs silencing was re‐established. Therefore, common sRNAs could control PRR/NLR genes and distal pericentromeric TEs and preferentially silence TEs when they are activated. Instituto de Biotecnología Fil: Cambiagno, Damián Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina Fil: Nota, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones En Química Biológica de Córdoba (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rius, Sebastián Pablo. Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Casati, Paula. Universidad Nacional de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Alvarez, Maria Elena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Química Biológica de Córdoba; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Quimica Biológica; Argentina |
| description |
Pattern recognition receptors (PRR) and nucleotide‐binding leucine‐rich repeat proteins (NLR) are major components of the plant immune system responsible for pathogen detection. To date, the transcriptional regulation of PRR/NLR genes is poorly understood. Some PRR/NLR genes are affected by epigenetic changes of neighboring transposable elements (TEs) (cis regulation). We analyzed whether these genes can also respond to changes in the epigenetic marks of distal pericentromeric TEs (trans regulation). We found that Arabidopsis tissues infected with Pseudomonas syringae pv. tomato (Pst) initially induced the expression of pericentromeric TEs, and then repressed it by RNA‐directed DNA methylation (RdDM). The latter response was accompanied by the accumulation of small RNAs (sRNAs) mapping to the TEs. Curiously these sRNAs also mapped to distal PRR/NLR genes, which were controlled by RdDM but remained induced in the infected tissues. Then, we used non‐infected mom1 (Morpheus’ molecule 1) mutants that expressed pericentromeric TEs to test if they lose repression of PRR/NLR genes. mom1 plants activated several PRR/NLR genes that were unlinked to MOM1‐targeted TEs, and showed enhanced resistance to Pst. Remarkably, the increased defenses of mom1 were abolished when MOM1/RdDM‐mediated pericentromeric TEs silencing was re‐established. Therefore, common sRNAs could control PRR/NLR genes and distal pericentromeric TEs and preferentially silence TEs when they are activated. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-12 2019-02-25T17:26:22Z 2019-02-25T17:26:22Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/20.500.12123/4488 1365-313X https://doi.org/10.1111/tpj.14098 |
| url |
http://hdl.handle.net/20.500.12123/4488 https://doi.org/10.1111/tpj.14098 |
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1365-313X |
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eng |
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eng |
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restrictedAccess |
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application/pdf |
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Wiley |
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Wiley |
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