Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower
- Autores
- Vega, Tatiana; Gil, Mercedes; Martin, Gabriela; Moschen, Sebastian Nicolas; Picardi, Liliana; Nestares, Graciela
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The nature of non-target-site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification-related proteins in NTSR in sunflower. Two sunflower inbred lines were used: HA 425, which is IMI resistant (Imisun), and HA 89, which is IMI susceptible. The growth response to the IMI herbicide imazethapyr in combination with the P450 inhibitors 1-aminobenzotriazole (ABT) or piperonyl butoxide (PBO) was evaluated in 15-d-old sunflower plantlets. Roots were collected, and label-free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. The increased phytotoxicity of imazethapyr observed in the resistant line when ABT or PBO were present agrees with the hypothesis that NTSR mechanisms may contribute to herbicide resistance in sunflower. The herbicide treatment also led to changes in the levels of biotic and abiotic stress-related proteins, glutathione S-transferases, and cytochrome P450s, among others. Plant growth and root protein expression response to IMI herbicides in sunflower would be a combination of stress-related and detoxification mechanisms. Understanding the basis of NTSR becomes helpful to exploit this trait in sunflower crop and to develop xenobiotic-resistant, soil-remediating cultivars.
EEA Famaillá
Fil: Vega, Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina
Fil: Vega, Tatiana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina
Fil: Gil, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina
Fil: Gil, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina
Fil: Martin, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina
Fil: Martin, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina
Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.
Fil: Moschen, Sebastian Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Picardi, Liliana. Universidad Nacional de Rosario. Consejo de Investigaciones; Argentina
Fil: Nestares, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina
Fil: Nestares, Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina - Fuente
- Crop Science 60 (4) : 1809-1822 (July-August 2020)
- Materia
-
Girasol
Herbicidas
Resistencia a los Herbicidas
Estres
Desintoxicación
Helianthus annuus
Sunflowers
Herbicides
Herbicide Resistance
Stress
Detoxification - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/11724
Ver los metadatos del registro completo
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Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflowerVega, TatianaGil, MercedesMartin, GabrielaMoschen, Sebastian NicolasPicardi, LilianaNestares, GracielaGirasolHerbicidasResistencia a los HerbicidasEstresDesintoxicaciónHelianthus annuusSunflowersHerbicidesHerbicide ResistanceStressDetoxificationThe nature of non-target-site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification-related proteins in NTSR in sunflower. Two sunflower inbred lines were used: HA 425, which is IMI resistant (Imisun), and HA 89, which is IMI susceptible. The growth response to the IMI herbicide imazethapyr in combination with the P450 inhibitors 1-aminobenzotriazole (ABT) or piperonyl butoxide (PBO) was evaluated in 15-d-old sunflower plantlets. Roots were collected, and label-free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. The increased phytotoxicity of imazethapyr observed in the resistant line when ABT or PBO were present agrees with the hypothesis that NTSR mechanisms may contribute to herbicide resistance in sunflower. The herbicide treatment also led to changes in the levels of biotic and abiotic stress-related proteins, glutathione S-transferases, and cytochrome P450s, among others. Plant growth and root protein expression response to IMI herbicides in sunflower would be a combination of stress-related and detoxification mechanisms. Understanding the basis of NTSR becomes helpful to exploit this trait in sunflower crop and to develop xenobiotic-resistant, soil-remediating cultivars.EEA FamailláFil: Vega, Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; ArgentinaFil: Vega, Tatiana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; ArgentinaFil: Gil, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; ArgentinaFil: Gil, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; ArgentinaFil: Martin, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; ArgentinaFil: Martin, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; ArgentinaFil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina.Fil: Moschen, Sebastian Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Picardi, Liliana. Universidad Nacional de Rosario. Consejo de Investigaciones; ArgentinaFil: Nestares, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; ArgentinaFil: Nestares, Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; ArgentinaWiley2022-04-25T14:29:49Z2022-04-25T14:29:49Z2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11724https://acsess.onlinelibrary.wiley.com/doi/abs/10.1002/csc2.201381435-0653https://doi.org/10.1002/csc2.20138Crop Science 60 (4) : 1809-1822 (July-August 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:17:56Zoai:localhost:20.500.12123/11724instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:56.773INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title |
Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| spellingShingle |
Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower Vega, Tatiana Girasol Herbicidas Resistencia a los Herbicidas Estres Desintoxicación Helianthus annuus Sunflowers Herbicides Herbicide Resistance Stress Detoxification |
| title_short |
Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_full |
Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_fullStr |
Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_full_unstemmed |
Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| title_sort |
Stress response and detoxification mechanisms involved in non-target-site herbicide resistance in sunflower |
| dc.creator.none.fl_str_mv |
Vega, Tatiana Gil, Mercedes Martin, Gabriela Moschen, Sebastian Nicolas Picardi, Liliana Nestares, Graciela |
| author |
Vega, Tatiana |
| author_facet |
Vega, Tatiana Gil, Mercedes Martin, Gabriela Moschen, Sebastian Nicolas Picardi, Liliana Nestares, Graciela |
| author_role |
author |
| author2 |
Gil, Mercedes Martin, Gabriela Moschen, Sebastian Nicolas Picardi, Liliana Nestares, Graciela |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Girasol Herbicidas Resistencia a los Herbicidas Estres Desintoxicación Helianthus annuus Sunflowers Herbicides Herbicide Resistance Stress Detoxification |
| topic |
Girasol Herbicidas Resistencia a los Herbicidas Estres Desintoxicación Helianthus annuus Sunflowers Herbicides Herbicide Resistance Stress Detoxification |
| dc.description.none.fl_txt_mv |
The nature of non-target-site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification-related proteins in NTSR in sunflower. Two sunflower inbred lines were used: HA 425, which is IMI resistant (Imisun), and HA 89, which is IMI susceptible. The growth response to the IMI herbicide imazethapyr in combination with the P450 inhibitors 1-aminobenzotriazole (ABT) or piperonyl butoxide (PBO) was evaluated in 15-d-old sunflower plantlets. Roots were collected, and label-free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. The increased phytotoxicity of imazethapyr observed in the resistant line when ABT or PBO were present agrees with the hypothesis that NTSR mechanisms may contribute to herbicide resistance in sunflower. The herbicide treatment also led to changes in the levels of biotic and abiotic stress-related proteins, glutathione S-transferases, and cytochrome P450s, among others. Plant growth and root protein expression response to IMI herbicides in sunflower would be a combination of stress-related and detoxification mechanisms. Understanding the basis of NTSR becomes helpful to exploit this trait in sunflower crop and to develop xenobiotic-resistant, soil-remediating cultivars. EEA Famaillá Fil: Vega, Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Vega, Tatiana. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Gil, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Gil, Mercedes. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Martin, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Martin, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Moschen, Sebastian Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Fil: Moschen, Sebastian Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Picardi, Liliana. Universidad Nacional de Rosario. Consejo de Investigaciones; Argentina Fil: Nestares, Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ciencias Agrarias; Argentina Fil: Nestares, Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias; Argentina |
| description |
The nature of non-target-site herbicide resistance (NTSR) to imidazolinone (IMI) in HA425 sunflower (Helianthus annuus L.) has not yet been fully characterized but could be related to xenobiotic metabolism. The objective of this study was to evaluate the role of cytochrome P450 monooxygenases (P450s) and other detoxification-related proteins in NTSR in sunflower. Two sunflower inbred lines were used: HA 425, which is IMI resistant (Imisun), and HA 89, which is IMI susceptible. The growth response to the IMI herbicide imazethapyr in combination with the P450 inhibitors 1-aminobenzotriazole (ABT) or piperonyl butoxide (PBO) was evaluated in 15-d-old sunflower plantlets. Roots were collected, and label-free quantitation (LFQ) proteomic analysis was carried out to characterize the NTSR mechanisms involved in the IMI resistance trait in sunflower. The increased phytotoxicity of imazethapyr observed in the resistant line when ABT or PBO were present agrees with the hypothesis that NTSR mechanisms may contribute to herbicide resistance in sunflower. The herbicide treatment also led to changes in the levels of biotic and abiotic stress-related proteins, glutathione S-transferases, and cytochrome P450s, among others. Plant growth and root protein expression response to IMI herbicides in sunflower would be a combination of stress-related and detoxification mechanisms. Understanding the basis of NTSR becomes helpful to exploit this trait in sunflower crop and to develop xenobiotic-resistant, soil-remediating cultivars. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-08 2022-04-25T14:29:49Z 2022-04-25T14:29:49Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
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http://hdl.handle.net/20.500.12123/11724 https://acsess.onlinelibrary.wiley.com/doi/abs/10.1002/csc2.20138 1435-0653 https://doi.org/10.1002/csc2.20138 |
| url |
http://hdl.handle.net/20.500.12123/11724 https://acsess.onlinelibrary.wiley.com/doi/abs/10.1002/csc2.20138 https://doi.org/10.1002/csc2.20138 |
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1435-0653 |
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eng |
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