First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing

Autores
Montoya, Jorgelina Ceferina; Fabressi, Esteban E.; Yanniccari, Marcos
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Kochia (Bassia scoparia (L.) A. J. Scott.) is a problematic weed. In Argentina, farmers have recently experienced poor control over imidazolinone-resistant sunflowers, specifically the sensitivity of putative kochia resistant plants to imidazolinone herbicides. Seeds from putative resistant populations from Pellegrini (P), De Bary (DB), and 30 de Agosto (A) and a known susceptible (S) population from Col. Martín Fierro were sown in pots. A mixture of imazapyr (Clearsol® WG 80%) and imazamox (Trigosol WG 70%) was sprayed when plants reached approximately 6 cm in height and 10-leaf stage using a boom sprayer. A trial was conducted to determine the response of kochia to the recommended doses (1x) of imazapyr 80.00 g ai ha-1 + imazamox 20.30 g ai ha-1. Plants were harvested 30 days after treatment to determine fresh shoot weight. A dose–response experiment was performed using imazapyr + imazamox at concentrations of 0x, 1/4x, 1/2x, 1x, 2x, 4x, and 8x. Partial ALS gene amplification was performed using leaves of plants that survived an 8x dose. The 1x fresh weight of the putative populations did not differ from 0x. LD50 of imazapyr+imazamox (g ai ha-1) for each biotype was: S, 18.86 + 4.79; DB, 82.48 + 20.93; A, 179.36 + 45.51; and P, 550.88 + 139.79. The three resistant populations exhibited a single-point mutation from guanine to thymine, detected in codon 574 of the ALS gene. This mutation confers resistance to ALS inhibitors. This is the first reported case of kochia resistance in Argentina, providing valuable insights that extend beyond sunflower weed management.
EEA Anguil
Fil: Montoya, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Fabressi, Esteban E. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Yanniccari, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Chilean Journal of Agricultural & Animal Science 41 (2) : 371-380. (August 2025)
Materia
Malezas
Herbicida con Imidazolinona
Herbicidas
Imazapir
Ensayo Biológico
Girasol
Weeds
Imidazolinone Herbicides
Herbicides
Imazamox
Imazapyr
Bio-Assays
Sunflowers
Bassia scoparia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23761
Acceder
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oai_identifier_str oai:localhost:20.500.12123/23761
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencingMontoya, Jorgelina CeferinaFabressi, Esteban E.Yanniccari, MarcosMalezasHerbicida con ImidazolinonaHerbicidasImazapirEnsayo BiológicoGirasolWeedsImidazolinone HerbicidesHerbicidesImazamoxImazapyrBio-AssaysSunflowersBassia scopariaKochia (Bassia scoparia (L.) A. J. Scott.) is a problematic weed. In Argentina, farmers have recently experienced poor control over imidazolinone-resistant sunflowers, specifically the sensitivity of putative kochia resistant plants to imidazolinone herbicides. Seeds from putative resistant populations from Pellegrini (P), De Bary (DB), and 30 de Agosto (A) and a known susceptible (S) population from Col. Martín Fierro were sown in pots. A mixture of imazapyr (Clearsol® WG 80%) and imazamox (Trigosol WG 70%) was sprayed when plants reached approximately 6 cm in height and 10-leaf stage using a boom sprayer. A trial was conducted to determine the response of kochia to the recommended doses (1x) of imazapyr 80.00 g ai ha-1 + imazamox 20.30 g ai ha-1. Plants were harvested 30 days after treatment to determine fresh shoot weight. A dose–response experiment was performed using imazapyr + imazamox at concentrations of 0x, 1/4x, 1/2x, 1x, 2x, 4x, and 8x. Partial ALS gene amplification was performed using leaves of plants that survived an 8x dose. The 1x fresh weight of the putative populations did not differ from 0x. LD50 of imazapyr+imazamox (g ai ha-1) for each biotype was: S, 18.86 + 4.79; DB, 82.48 + 20.93; A, 179.36 + 45.51; and P, 550.88 + 139.79. The three resistant populations exhibited a single-point mutation from guanine to thymine, detected in codon 574 of the ALS gene. This mutation confers resistance to ALS inhibitors. This is the first reported case of kochia resistance in Argentina, providing valuable insights that extend beyond sunflower weed management.EEA AnguilFil: Montoya, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Fabressi, Esteban E. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Yanniccari, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Yanniccari, Marcos. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaUniversidad de Concepción, Chile2025-09-10T14:16:52Z2025-09-10T14:16:52Z2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23761https://revistas.udec.cl/index.php/chjaas/article/view/202560719-38820719-3890https://doi.org/10.29393/CHJAAS41-33FRFA30033Chilean Journal of Agricultural & Animal Science 41 (2) : 371-380. (August 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I094, Gestión ambiental de los agroquímicos: manejo, mitigación y remediacióninfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:31Zoai:localhost:20.500.12123/23761instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:32.048INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing
title First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing
spellingShingle First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing
Montoya, Jorgelina Ceferina
Malezas
Herbicida con Imidazolinona
Herbicidas
Imazapir
Ensayo Biológico
Girasol
Weeds
Imidazolinone Herbicides
Herbicides
Imazamox
Imazapyr
Bio-Assays
Sunflowers
Bassia scoparia
title_short First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing
title_full First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing
title_fullStr First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing
title_full_unstemmed First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing
title_sort First report of imidazolinone-resistant Bassia scoparia in Argentina : evidence from dose-response bioassays and ALS gene sequencing
dc.creator.none.fl_str_mv Montoya, Jorgelina Ceferina
Fabressi, Esteban E.
Yanniccari, Marcos
author Montoya, Jorgelina Ceferina
author_facet Montoya, Jorgelina Ceferina
Fabressi, Esteban E.
Yanniccari, Marcos
author_role author
author2 Fabressi, Esteban E.
Yanniccari, Marcos
author2_role author
author
dc.subject.none.fl_str_mv Malezas
Herbicida con Imidazolinona
Herbicidas
Imazapir
Ensayo Biológico
Girasol
Weeds
Imidazolinone Herbicides
Herbicides
Imazamox
Imazapyr
Bio-Assays
Sunflowers
Bassia scoparia
topic Malezas
Herbicida con Imidazolinona
Herbicidas
Imazapir
Ensayo Biológico
Girasol
Weeds
Imidazolinone Herbicides
Herbicides
Imazamox
Imazapyr
Bio-Assays
Sunflowers
Bassia scoparia
dc.description.none.fl_txt_mv Kochia (Bassia scoparia (L.) A. J. Scott.) is a problematic weed. In Argentina, farmers have recently experienced poor control over imidazolinone-resistant sunflowers, specifically the sensitivity of putative kochia resistant plants to imidazolinone herbicides. Seeds from putative resistant populations from Pellegrini (P), De Bary (DB), and 30 de Agosto (A) and a known susceptible (S) population from Col. Martín Fierro were sown in pots. A mixture of imazapyr (Clearsol® WG 80%) and imazamox (Trigosol WG 70%) was sprayed when plants reached approximately 6 cm in height and 10-leaf stage using a boom sprayer. A trial was conducted to determine the response of kochia to the recommended doses (1x) of imazapyr 80.00 g ai ha-1 + imazamox 20.30 g ai ha-1. Plants were harvested 30 days after treatment to determine fresh shoot weight. A dose–response experiment was performed using imazapyr + imazamox at concentrations of 0x, 1/4x, 1/2x, 1x, 2x, 4x, and 8x. Partial ALS gene amplification was performed using leaves of plants that survived an 8x dose. The 1x fresh weight of the putative populations did not differ from 0x. LD50 of imazapyr+imazamox (g ai ha-1) for each biotype was: S, 18.86 + 4.79; DB, 82.48 + 20.93; A, 179.36 + 45.51; and P, 550.88 + 139.79. The three resistant populations exhibited a single-point mutation from guanine to thymine, detected in codon 574 of the ALS gene. This mutation confers resistance to ALS inhibitors. This is the first reported case of kochia resistance in Argentina, providing valuable insights that extend beyond sunflower weed management.
EEA Anguil
Fil: Montoya, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Fabressi, Esteban E. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Yanniccari, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Kochia (Bassia scoparia (L.) A. J. Scott.) is a problematic weed. In Argentina, farmers have recently experienced poor control over imidazolinone-resistant sunflowers, specifically the sensitivity of putative kochia resistant plants to imidazolinone herbicides. Seeds from putative resistant populations from Pellegrini (P), De Bary (DB), and 30 de Agosto (A) and a known susceptible (S) population from Col. Martín Fierro were sown in pots. A mixture of imazapyr (Clearsol® WG 80%) and imazamox (Trigosol WG 70%) was sprayed when plants reached approximately 6 cm in height and 10-leaf stage using a boom sprayer. A trial was conducted to determine the response of kochia to the recommended doses (1x) of imazapyr 80.00 g ai ha-1 + imazamox 20.30 g ai ha-1. Plants were harvested 30 days after treatment to determine fresh shoot weight. A dose–response experiment was performed using imazapyr + imazamox at concentrations of 0x, 1/4x, 1/2x, 1x, 2x, 4x, and 8x. Partial ALS gene amplification was performed using leaves of plants that survived an 8x dose. The 1x fresh weight of the putative populations did not differ from 0x. LD50 of imazapyr+imazamox (g ai ha-1) for each biotype was: S, 18.86 + 4.79; DB, 82.48 + 20.93; A, 179.36 + 45.51; and P, 550.88 + 139.79. The three resistant populations exhibited a single-point mutation from guanine to thymine, detected in codon 574 of the ALS gene. This mutation confers resistance to ALS inhibitors. This is the first reported case of kochia resistance in Argentina, providing valuable insights that extend beyond sunflower weed management.
publishDate 2025
dc.date.none.fl_str_mv 2025-09-10T14:16:52Z
2025-09-10T14:16:52Z
2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23761
https://revistas.udec.cl/index.php/chjaas/article/view/20256
0719-3882
0719-3890
https://doi.org/10.29393/CHJAAS41-33FRFA30033
url http://hdl.handle.net/20.500.12123/23761
https://revistas.udec.cl/index.php/chjaas/article/view/20256
https://doi.org/10.29393/CHJAAS41-33FRFA30033
identifier_str_mv 0719-3882
0719-3890
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I094, Gestión ambiental de los agroquímicos: manejo, mitigación y remediación
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Concepción, Chile
publisher.none.fl_str_mv Universidad de Concepción, Chile
dc.source.none.fl_str_mv Chilean Journal of Agricultural & Animal Science 41 (2) : 371-380. (August 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.558318

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