Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower

Autores
Gil, Mercedes; Ochogavía, Ana Claudia; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Nestares, Graciela María
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Imidazolinone resistance found in a wild sunflower (Helianthus annuus L.) population was successfully transferred to a cultivated inbred line developing ‘Imisun’ sunflowers. Genetic regulation of this trait has been reported to involve two genes: Imr1, an allelic variant of ahasl1 locus that codes for acetohydroxyacid synthase catalytic subunit, and the modifier Imr2, whose identity remains unknown, but it could be related to non-target-site resistance such as xenobiotic metabolism. The aim of the present study was to characterize the gene expression of resistant and susceptible sunflower lines in response to imazethapyr herbicide by complementary DNA amplified fragment-length polymorphism (cDNA-AFLP). Three assays were performed to determine (i) optimal herbicide treatment concentration, (ii) duration of herbicide treatment, and (iii) in vitro acetohydroxyacid synthase activity to assess enzyme inhibition levels. An important number of genes related to metabolism of xeno-biotics and stress was found: cytochrome P450 monooxygenases, UDP-glucuronosyl/UDP-glucosyltransferases, glycosyltransferases, and ATP-binding cassette transporters, among others. These results suggest that non-target-site resistance mechanisms may contribute to herbicide resistance in Imisun sunflower and could be related to the modifier gene Imr2. Using cDNA-AFLP, we were able to detect candidate detoxification-related genes potentially involved in imidazolinone resistance in sunflower.
Fil: Gil, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Vega, Tatiana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Felitti, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Nestares, Graciela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
HELIANTHUS ANNUUS L
IMAZETHAPYR
HERBICIDE RESISTANCE
GENE EXPRESSION
CDNA-AFLP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/91965
Acceder
id CONICETDig_af8badd25c541bb0373bd3cbeb984e82
oai_identifier_str oai:ri.conicet.gov.ar:11336/91965
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Transcript profiling of non-target-site imidazolinone resistance in imisun sunflowerGil, MercedesOchogavía, Ana ClaudiaVega, Tatiana AlejandraFelitti, Silvina AndreaNestares, Graciela MaríaHELIANTHUS ANNUUS LIMAZETHAPYRHERBICIDE RESISTANCEGENE EXPRESSIONCDNA-AFLPhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Imidazolinone resistance found in a wild sunflower (Helianthus annuus L.) population was successfully transferred to a cultivated inbred line developing ‘Imisun’ sunflowers. Genetic regulation of this trait has been reported to involve two genes: Imr1, an allelic variant of ahasl1 locus that codes for acetohydroxyacid synthase catalytic subunit, and the modifier Imr2, whose identity remains unknown, but it could be related to non-target-site resistance such as xenobiotic metabolism. The aim of the present study was to characterize the gene expression of resistant and susceptible sunflower lines in response to imazethapyr herbicide by complementary DNA amplified fragment-length polymorphism (cDNA-AFLP). Three assays were performed to determine (i) optimal herbicide treatment concentration, (ii) duration of herbicide treatment, and (iii) in vitro acetohydroxyacid synthase activity to assess enzyme inhibition levels. An important number of genes related to metabolism of xeno-biotics and stress was found: cytochrome P450 monooxygenases, UDP-glucuronosyl/UDP-glucosyltransferases, glycosyltransferases, and ATP-binding cassette transporters, among others. These results suggest that non-target-site resistance mechanisms may contribute to herbicide resistance in Imisun sunflower and could be related to the modifier gene Imr2. Using cDNA-AFLP, we were able to detect candidate detoxification-related genes potentially involved in imidazolinone resistance in sunflower.Fil: Gil, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Vega, Tatiana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Felitti, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Nestares, Graciela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaCrop Science Society of America2018-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/91965Gil, Mercedes; Ochogavía, Ana Claudia; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Nestares, Graciela María; Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower; Crop Science Society of America; Crop Science; 58; 5; 9-2018; 1991-20010011-183XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/58/5/1991info:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2018.01.0074info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:49Zoai:ri.conicet.gov.ar:11336/91965instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:50.177CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower
title Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower
spellingShingle Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower
Gil, Mercedes
HELIANTHUS ANNUUS L
IMAZETHAPYR
HERBICIDE RESISTANCE
GENE EXPRESSION
CDNA-AFLP
title_short Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower
title_full Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower
title_fullStr Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower
title_full_unstemmed Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower
title_sort Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower
dc.creator.none.fl_str_mv Gil, Mercedes
Ochogavía, Ana Claudia
Vega, Tatiana Alejandra
Felitti, Silvina Andrea
Nestares, Graciela María
author Gil, Mercedes
author_facet Gil, Mercedes
Ochogavía, Ana Claudia
Vega, Tatiana Alejandra
Felitti, Silvina Andrea
Nestares, Graciela María
author_role author
author2 Ochogavía, Ana Claudia
Vega, Tatiana Alejandra
Felitti, Silvina Andrea
Nestares, Graciela María
author2_role author
author
author
author
dc.subject.none.fl_str_mv HELIANTHUS ANNUUS L
IMAZETHAPYR
HERBICIDE RESISTANCE
GENE EXPRESSION
CDNA-AFLP
topic HELIANTHUS ANNUUS L
IMAZETHAPYR
HERBICIDE RESISTANCE
GENE EXPRESSION
CDNA-AFLP
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Imidazolinone resistance found in a wild sunflower (Helianthus annuus L.) population was successfully transferred to a cultivated inbred line developing ‘Imisun’ sunflowers. Genetic regulation of this trait has been reported to involve two genes: Imr1, an allelic variant of ahasl1 locus that codes for acetohydroxyacid synthase catalytic subunit, and the modifier Imr2, whose identity remains unknown, but it could be related to non-target-site resistance such as xenobiotic metabolism. The aim of the present study was to characterize the gene expression of resistant and susceptible sunflower lines in response to imazethapyr herbicide by complementary DNA amplified fragment-length polymorphism (cDNA-AFLP). Three assays were performed to determine (i) optimal herbicide treatment concentration, (ii) duration of herbicide treatment, and (iii) in vitro acetohydroxyacid synthase activity to assess enzyme inhibition levels. An important number of genes related to metabolism of xeno-biotics and stress was found: cytochrome P450 monooxygenases, UDP-glucuronosyl/UDP-glucosyltransferases, glycosyltransferases, and ATP-binding cassette transporters, among others. These results suggest that non-target-site resistance mechanisms may contribute to herbicide resistance in Imisun sunflower and could be related to the modifier gene Imr2. Using cDNA-AFLP, we were able to detect candidate detoxification-related genes potentially involved in imidazolinone resistance in sunflower.
Fil: Gil, Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Ochogavía, Ana Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Vega, Tatiana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Felitti, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Nestares, Graciela María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description Imidazolinone resistance found in a wild sunflower (Helianthus annuus L.) population was successfully transferred to a cultivated inbred line developing ‘Imisun’ sunflowers. Genetic regulation of this trait has been reported to involve two genes: Imr1, an allelic variant of ahasl1 locus that codes for acetohydroxyacid synthase catalytic subunit, and the modifier Imr2, whose identity remains unknown, but it could be related to non-target-site resistance such as xenobiotic metabolism. The aim of the present study was to characterize the gene expression of resistant and susceptible sunflower lines in response to imazethapyr herbicide by complementary DNA amplified fragment-length polymorphism (cDNA-AFLP). Three assays were performed to determine (i) optimal herbicide treatment concentration, (ii) duration of herbicide treatment, and (iii) in vitro acetohydroxyacid synthase activity to assess enzyme inhibition levels. An important number of genes related to metabolism of xeno-biotics and stress was found: cytochrome P450 monooxygenases, UDP-glucuronosyl/UDP-glucosyltransferases, glycosyltransferases, and ATP-binding cassette transporters, among others. These results suggest that non-target-site resistance mechanisms may contribute to herbicide resistance in Imisun sunflower and could be related to the modifier gene Imr2. Using cDNA-AFLP, we were able to detect candidate detoxification-related genes potentially involved in imidazolinone resistance in sunflower.
publishDate 2018
dc.date.none.fl_str_mv 2018-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/91965
Gil, Mercedes; Ochogavía, Ana Claudia; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Nestares, Graciela María; Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower; Crop Science Society of America; Crop Science; 58; 5; 9-2018; 1991-2001
0011-183X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/91965
identifier_str_mv Gil, Mercedes; Ochogavía, Ana Claudia; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Nestares, Graciela María; Transcript profiling of non-target-site imidazolinone resistance in imisun sunflower; Crop Science Society of America; Crop Science; 58; 5; 9-2018; 1991-2001
0011-183X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://dl.sciencesocieties.org/publications/cs/abstracts/58/5/1991
info:eu-repo/semantics/altIdentifier/doi/10.2135/cropsci2018.01.0074
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Crop Science Society of America
publisher.none.fl_str_mv Crop Science Society of America
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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