The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)

Autores
Aguilera, Patricia Mabel; Grabiele, Mauro; Debat, Humberto Julio; Bubillo, Rosana Elizabeth; Martí, Dardo Andrea
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We present the first report of the transcribed 18S–25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named “mate”. Next-generation sequencing data were assembled, reference-annotated and further characterized. The assembled consensus sequence of the complete transcribed rDNA unit is 6961 nt, with a coverage of ∼18,646X and 99.3% of global sequence similarity. The transcribed 18S and 25S rDNA genes of yerba mate are the first in the genus to be characterized, combined with the 5.8S that is identical to the consensus sequence of Ilex. The ITS1 and the ITS2 display species-specific regions, useful to differentiate yerba mate from adulterant taxa of the manufactured product. A replication slippage process seems significant in the evolution of the internal transcribed spacer in Ilex. The 5-external transcribed spacer consists of SR1, which encloses a large inverted repeat/hairpin region, and SR2, which is a high homology region in the Asterids clade and embrace six distinctive inverted repeat/hairpin segments. The 3ETS presents a pyrimidine-rich block associated with transcription termination, mirror-like palindromes and repetitive segments, all networking at the secondary structure level. This information will be helpful in carrying out future studies in the molecular evolution characterization of this crop and related species of Ilex.
Instituto de Patología Vegetal
EEA Cerro Azul
Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina
Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bubillo, Rosana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
Fil: Martí, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina
Fuente
Plant Biosystems 150 (6) : 1240-1248 (2016)
Materia
Ilex paraguariensis
ARN
ARN Ribosomial
Ribosomal RNA
Secuencia Nucleotídica
Genética
Mate
RNA
Nucleotide Sequence
Genetics
Yerba Mate
Secuencia Genética
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2285
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2285
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)Aguilera, Patricia MabelGrabiele, MauroDebat, Humberto JulioBubillo, Rosana ElizabethMartí, Dardo AndreaIlex paraguariensisARNARN RibosomialRibosomal RNASecuencia NucleotídicaGenéticaMateRNANucleotide SequenceGeneticsYerba MateSecuencia GenéticaWe present the first report of the transcribed 18S–25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named “mate”. Next-generation sequencing data were assembled, reference-annotated and further characterized. The assembled consensus sequence of the complete transcribed rDNA unit is 6961 nt, with a coverage of ∼18,646X and 99.3% of global sequence similarity. The transcribed 18S and 25S rDNA genes of yerba mate are the first in the genus to be characterized, combined with the 5.8S that is identical to the consensus sequence of Ilex. The ITS1 and the ITS2 display species-specific regions, useful to differentiate yerba mate from adulterant taxa of the manufactured product. A replication slippage process seems significant in the evolution of the internal transcribed spacer in Ilex. The 5-external transcribed spacer consists of SR1, which encloses a large inverted repeat/hairpin region, and SR2, which is a high homology region in the Asterids clade and embrace six distinctive inverted repeat/hairpin segments. The 3ETS presents a pyrimidine-rich block associated with transcription termination, mirror-like palindromes and repetitive segments, all networking at the secondary structure level. This information will be helpful in carrying out future studies in the molecular evolution characterization of this crop and related species of Ilex.Instituto de Patología VegetalEEA Cerro AzulFil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; ArgentinaFil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; ArgentinaFil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Bubillo, Rosana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; ArgentinaFil: Martí, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina2018-04-23T15:09:55Z2018-04-23T15:09:55Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.tandfonline.com/doi/full/10.1080/11263504.2015.1018982http://hdl.handle.net/20.500.12123/22851126-35041724-5575https://doi.org/10.1080/11263504.2015.1018982Plant Biosystems 150 (6) : 1240-1248 (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:12Zoai:localhost:20.500.12123/2285instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:13.331INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
spellingShingle The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
Aguilera, Patricia Mabel
Ilex paraguariensis
ARN
ARN Ribosomial
Ribosomal RNA
Secuencia Nucleotídica
Genética
Mate
RNA
Nucleotide Sequence
Genetics
Yerba Mate
Secuencia Genética
title_short The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title_full The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title_fullStr The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title_full_unstemmed The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
title_sort The 18S–25S ribosomal RNA unit of yerba mate (Ilex paraguariensis A. St.-Hil.)
dc.creator.none.fl_str_mv Aguilera, Patricia Mabel
Grabiele, Mauro
Debat, Humberto Julio
Bubillo, Rosana Elizabeth
Martí, Dardo Andrea
author Aguilera, Patricia Mabel
author_facet Aguilera, Patricia Mabel
Grabiele, Mauro
Debat, Humberto Julio
Bubillo, Rosana Elizabeth
Martí, Dardo Andrea
author_role author
author2 Grabiele, Mauro
Debat, Humberto Julio
Bubillo, Rosana Elizabeth
Martí, Dardo Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ilex paraguariensis
ARN
ARN Ribosomial
Ribosomal RNA
Secuencia Nucleotídica
Genética
Mate
RNA
Nucleotide Sequence
Genetics
Yerba Mate
Secuencia Genética
topic Ilex paraguariensis
ARN
ARN Ribosomial
Ribosomal RNA
Secuencia Nucleotídica
Genética
Mate
RNA
Nucleotide Sequence
Genetics
Yerba Mate
Secuencia Genética
dc.description.none.fl_txt_mv We present the first report of the transcribed 18S–25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named “mate”. Next-generation sequencing data were assembled, reference-annotated and further characterized. The assembled consensus sequence of the complete transcribed rDNA unit is 6961 nt, with a coverage of ∼18,646X and 99.3% of global sequence similarity. The transcribed 18S and 25S rDNA genes of yerba mate are the first in the genus to be characterized, combined with the 5.8S that is identical to the consensus sequence of Ilex. The ITS1 and the ITS2 display species-specific regions, useful to differentiate yerba mate from adulterant taxa of the manufactured product. A replication slippage process seems significant in the evolution of the internal transcribed spacer in Ilex. The 5-external transcribed spacer consists of SR1, which encloses a large inverted repeat/hairpin region, and SR2, which is a high homology region in the Asterids clade and embrace six distinctive inverted repeat/hairpin segments. The 3ETS presents a pyrimidine-rich block associated with transcription termination, mirror-like palindromes and repetitive segments, all networking at the secondary structure level. This information will be helpful in carrying out future studies in the molecular evolution characterization of this crop and related species of Ilex.
Instituto de Patología Vegetal
EEA Cerro Azul
Fil: Aguilera, Patricia Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina
Fil: Grabiele, Mauro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina
Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Bubillo, Rosana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Cerro Azul; Argentina
Fil: Martí, Dardo Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Universidad Nacional de Misiones. Instituto de Biología Subtropical; Argentina. Universidad Nacional de Misiones. Instituto de Biotecnología de Misiones; Argentina
description We present the first report of the transcribed 18S–25S ribosomal DNA (rDNA) unit of the South American crop Ilex paraguariensis. This tree is consumed as an infusion named “mate”. Next-generation sequencing data were assembled, reference-annotated and further characterized. The assembled consensus sequence of the complete transcribed rDNA unit is 6961 nt, with a coverage of ∼18,646X and 99.3% of global sequence similarity. The transcribed 18S and 25S rDNA genes of yerba mate are the first in the genus to be characterized, combined with the 5.8S that is identical to the consensus sequence of Ilex. The ITS1 and the ITS2 display species-specific regions, useful to differentiate yerba mate from adulterant taxa of the manufactured product. A replication slippage process seems significant in the evolution of the internal transcribed spacer in Ilex. The 5-external transcribed spacer consists of SR1, which encloses a large inverted repeat/hairpin region, and SR2, which is a high homology region in the Asterids clade and embrace six distinctive inverted repeat/hairpin segments. The 3ETS presents a pyrimidine-rich block associated with transcription termination, mirror-like palindromes and repetitive segments, all networking at the secondary structure level. This information will be helpful in carrying out future studies in the molecular evolution characterization of this crop and related species of Ilex.
publishDate 2016
dc.date.none.fl_str_mv 2016
2018-04-23T15:09:55Z
2018-04-23T15:09:55Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.tandfonline.com/doi/full/10.1080/11263504.2015.1018982
http://hdl.handle.net/20.500.12123/2285
1126-3504
1724-5575
https://doi.org/10.1080/11263504.2015.1018982
url https://www.tandfonline.com/doi/full/10.1080/11263504.2015.1018982
http://hdl.handle.net/20.500.12123/2285
https://doi.org/10.1080/11263504.2015.1018982
identifier_str_mv 1126-3504
1724-5575
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plant Biosystems 150 (6) : 1240-1248 (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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