Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique

Autores
Liotino, Micaela; Varangot, Agostina; Beznec, Aylin; Auteri, Micol; Bossio, Adrian Ezequiel; Lewi, Dalia Marcela; Faccio, Paula Daniela
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Embryo rescue techniques have been used as an approach to raise hybrids from incompatible crosses. These techniques have also proved to be valuable tools for maize improvement, since they allow reducing the duration of the generation cycles for speed breeding. The aim of this study was to identify an efficient embryo rescue protocol to evaluate the response of maize (Zea mays L.) embryo culture and compare its generation time (seed to seed) with the generation time of plants obtained by mature seed germination. To this purpose, we evaluated the germination efficiency, in vitro protocol efficiency, and days to flowering of three advanced maize inbred lines developed at the National Institute of Agricultural Technology of Argentina. A greenhouse traditional strategy was used as control. The embryo rescue technique allowed obtaining nearly four generations per year compared to the two generations obtained in greenhouse. All the plants obtained by the embryo rescue technique were morphologically normal and fertile. The results confirmed the possibility of using embryo rescue strategies to significantly reduce the duration of the generation cycles in maize.
Instituto de Genética
Fil: Liotino, Micaela. Universidad de Morón. Facultad de Agronomia y Ciencias Agroalimentarias; Argentina
Fil: Varangot, Agostina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Auteri, Micol. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fuente
Maydica 64 (2) : M15. (Noviembre 2019)
Materia
In Vitro Culture
Maize
Plant Genetics
Plant Embryos
Culture Techniques
Cultivo in Vitro
Maíz
Zea Mays
Fitogenética
Embriones Vegetales
Técnicas de Cultivo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6700
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oai_identifier_str oai:localhost:20.500.12123/6700
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spelling Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue techniqueLiotino, MicaelaVarangot, AgostinaBeznec, AylinAuteri, MicolBossio, Adrian EzequielLewi, Dalia MarcelaFaccio, Paula DanielaIn Vitro CultureMaizePlant GeneticsPlant EmbryosCulture TechniquesCultivo in VitroMaízZea MaysFitogenéticaEmbriones VegetalesTécnicas de CultivoEmbryo rescue techniques have been used as an approach to raise hybrids from incompatible crosses. These techniques have also proved to be valuable tools for maize improvement, since they allow reducing the duration of the generation cycles for speed breeding. The aim of this study was to identify an efficient embryo rescue protocol to evaluate the response of maize (Zea mays L.) embryo culture and compare its generation time (seed to seed) with the generation time of plants obtained by mature seed germination. To this purpose, we evaluated the germination efficiency, in vitro protocol efficiency, and days to flowering of three advanced maize inbred lines developed at the National Institute of Agricultural Technology of Argentina. A greenhouse traditional strategy was used as control. The embryo rescue technique allowed obtaining nearly four generations per year compared to the two generations obtained in greenhouse. All the plants obtained by the embryo rescue technique were morphologically normal and fertile. The results confirmed the possibility of using embryo rescue strategies to significantly reduce the duration of the generation cycles in maize.Instituto de GenéticaFil: Liotino, Micaela. Universidad de Morón. Facultad de Agronomia y Ciencias Agroalimentarias; ArgentinaFil: Varangot, Agostina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Auteri, Micol. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaFil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; ArgentinaIstituto Sperimentale per la Cerealicoltura2020-02-10T13:26:57Z2020-02-10T13:26:57Z2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6700https://journals-crea.4science.it/index.php/maydica/article/view/19182279-8013Maydica 64 (2) : M15. (Noviembre 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-12-26T11:10:28Zoai:localhost:20.500.12123/6700instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-12-26 11:10:29.005INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
title Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
spellingShingle Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
Liotino, Micaela
In Vitro Culture
Maize
Plant Genetics
Plant Embryos
Culture Techniques
Cultivo in Vitro
Maíz
Zea Mays
Fitogenética
Embriones Vegetales
Técnicas de Cultivo
title_short Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
title_full Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
title_fullStr Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
title_full_unstemmed Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
title_sort Shortening of generation cycles in inbred lines of maize (Zea mays L.) through embryo rescue technique
dc.creator.none.fl_str_mv Liotino, Micaela
Varangot, Agostina
Beznec, Aylin
Auteri, Micol
Bossio, Adrian Ezequiel
Lewi, Dalia Marcela
Faccio, Paula Daniela
author Liotino, Micaela
author_facet Liotino, Micaela
Varangot, Agostina
Beznec, Aylin
Auteri, Micol
Bossio, Adrian Ezequiel
Lewi, Dalia Marcela
Faccio, Paula Daniela
author_role author
author2 Varangot, Agostina
Beznec, Aylin
Auteri, Micol
Bossio, Adrian Ezequiel
Lewi, Dalia Marcela
Faccio, Paula Daniela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv In Vitro Culture
Maize
Plant Genetics
Plant Embryos
Culture Techniques
Cultivo in Vitro
Maíz
Zea Mays
Fitogenética
Embriones Vegetales
Técnicas de Cultivo
topic In Vitro Culture
Maize
Plant Genetics
Plant Embryos
Culture Techniques
Cultivo in Vitro
Maíz
Zea Mays
Fitogenética
Embriones Vegetales
Técnicas de Cultivo
dc.description.none.fl_txt_mv Embryo rescue techniques have been used as an approach to raise hybrids from incompatible crosses. These techniques have also proved to be valuable tools for maize improvement, since they allow reducing the duration of the generation cycles for speed breeding. The aim of this study was to identify an efficient embryo rescue protocol to evaluate the response of maize (Zea mays L.) embryo culture and compare its generation time (seed to seed) with the generation time of plants obtained by mature seed germination. To this purpose, we evaluated the germination efficiency, in vitro protocol efficiency, and days to flowering of three advanced maize inbred lines developed at the National Institute of Agricultural Technology of Argentina. A greenhouse traditional strategy was used as control. The embryo rescue technique allowed obtaining nearly four generations per year compared to the two generations obtained in greenhouse. All the plants obtained by the embryo rescue technique were morphologically normal and fertile. The results confirmed the possibility of using embryo rescue strategies to significantly reduce the duration of the generation cycles in maize.
Instituto de Genética
Fil: Liotino, Micaela. Universidad de Morón. Facultad de Agronomia y Ciencias Agroalimentarias; Argentina
Fil: Varangot, Agostina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Beznec, Ailin. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Auteri, Micol. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Bossio, Adrian Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
Fil: Lewi, Dalia Marcela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Faccio, Paula Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina
description Embryo rescue techniques have been used as an approach to raise hybrids from incompatible crosses. These techniques have also proved to be valuable tools for maize improvement, since they allow reducing the duration of the generation cycles for speed breeding. The aim of this study was to identify an efficient embryo rescue protocol to evaluate the response of maize (Zea mays L.) embryo culture and compare its generation time (seed to seed) with the generation time of plants obtained by mature seed germination. To this purpose, we evaluated the germination efficiency, in vitro protocol efficiency, and days to flowering of three advanced maize inbred lines developed at the National Institute of Agricultural Technology of Argentina. A greenhouse traditional strategy was used as control. The embryo rescue technique allowed obtaining nearly four generations per year compared to the two generations obtained in greenhouse. All the plants obtained by the embryo rescue technique were morphologically normal and fertile. The results confirmed the possibility of using embryo rescue strategies to significantly reduce the duration of the generation cycles in maize.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
2020-02-10T13:26:57Z
2020-02-10T13:26:57Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6700
https://journals-crea.4science.it/index.php/maydica/article/view/1918
2279-8013
url http://hdl.handle.net/20.500.12123/6700
https://journals-crea.4science.it/index.php/maydica/article/view/1918
identifier_str_mv 2279-8013
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Istituto Sperimentale per la Cerealicoltura
publisher.none.fl_str_mv Istituto Sperimentale per la Cerealicoltura
dc.source.none.fl_str_mv Maydica 64 (2) : M15. (Noviembre 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1852582542893383680
score 13.075124

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