Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves

Autores
Sequin, Christian J.; Appelhans, Stefania Carolina; Heis, Magalí S.; Torrent, Walter A.; Trossero, Julia A.; Catalan, Cesar Atilio Nazareno; Sampietro, Diego Alejandro; Aceñolaza, Pablo Gilberto
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of synthetic fungicides for control soybean crop diseases has been cuestionated because of its impacts on human health and the environment. This work evaluated the composition, antifungal activity and toxicity of the alkaloids fraction obtained from Neltuma nigra leaves and compared its effect with the fungicide difenoconazole on Sclerotium rolfsii, Cercospora kikuchii, and C. sojina. Alkaloids fraction was extracted from leaves of N. nigra and its composition was analyzed by gas-chromatography coupled to mass spectrometry (GC-MS). The antifungal effect was recorded by measuring diameters of halos of inhibition of the growth of the different evaluated fungis. The toxicity of the alkaloids fraction was carried out using the method of Caenorhabditis elegans toxicity test. The extracted alkaloids fraction contained the alkaloids tryptamine (63.9%), tetrahydroxyharman (13.8%), phenethylamine (11.1%), cassine (10.4%), and N-formylphenylethylamine (0.8%). The alkaloids fraction was as effective against S. rolfsii as on the Cercospora strains, with minimum inhibitory doses of 25.0 μg disc-1 and inhibitory halos in the range of 8.5-13.0 mm, while difenoconazole had no effect on S. rolfsii. The toxicity of the alkaloid fraction (30.4-68.8 mg L-1) and difenoconazole (0.1-2.8 mg L-1) was tested on the reproduction, body length, and fertility of C. elegans. The fraction reduced the reproductive rate by 28.8-72.4% whereas difenoconazole did it from 12.2% to complete suppression. Neither of them affected the fertility of C. elegans. These findings suggest that the alkaloids fraction of N. nigra leaves is less toxic than difenoconazole and is a promising botanical fungicide against pathogenic soybean fungi.
EEA Paraná
Fil: Sequin, Christian J. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Appelhans, Estefania Carolina. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Appelhans, Estefania Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina
Fil: Heis, Magalí S. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Torrent, Walter A. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Trossero, Julia A. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Catalan, Cesar Atilio Nazareno. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. LABIFITO; Argentina
Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. LABIFITO; Argentina
Fil: Aceñolaza, Pablo Gilberto. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fuente
Biocatalysis and Agricultural Biotechnology 54 : 102914. (November 2023)
Materia
Antifungal Plants
Antifungal Properties
Alkaloids
Toxicology
Propiedades Antimicosicas
Alcaloides
Fabaceae
Toxicología
Neltuma nigra
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15939
Acceder
id INTADig_0405ef425915f5304a1bd3be87b4b4e9
oai_identifier_str oai:localhost:20.500.12123/15939
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leavesSequin, Christian J.Appelhans, Stefania CarolinaHeis, Magalí S.Torrent, Walter A.Trossero, Julia A.Catalan, Cesar Atilio NazarenoSampietro, Diego AlejandroAceñolaza, Pablo GilbertoAntifungal PlantsAntifungal PropertiesAlkaloidsToxicologyPropiedades AntimicosicasAlcaloidesFabaceaeToxicologíaNeltuma nigraThe use of synthetic fungicides for control soybean crop diseases has been cuestionated because of its impacts on human health and the environment. This work evaluated the composition, antifungal activity and toxicity of the alkaloids fraction obtained from Neltuma nigra leaves and compared its effect with the fungicide difenoconazole on Sclerotium rolfsii, Cercospora kikuchii, and C. sojina. Alkaloids fraction was extracted from leaves of N. nigra and its composition was analyzed by gas-chromatography coupled to mass spectrometry (GC-MS). The antifungal effect was recorded by measuring diameters of halos of inhibition of the growth of the different evaluated fungis. The toxicity of the alkaloids fraction was carried out using the method of Caenorhabditis elegans toxicity test. The extracted alkaloids fraction contained the alkaloids tryptamine (63.9%), tetrahydroxyharman (13.8%), phenethylamine (11.1%), cassine (10.4%), and N-formylphenylethylamine (0.8%). The alkaloids fraction was as effective against S. rolfsii as on the Cercospora strains, with minimum inhibitory doses of 25.0 μg disc-1 and inhibitory halos in the range of 8.5-13.0 mm, while difenoconazole had no effect on S. rolfsii. The toxicity of the alkaloid fraction (30.4-68.8 mg L-1) and difenoconazole (0.1-2.8 mg L-1) was tested on the reproduction, body length, and fertility of C. elegans. The fraction reduced the reproductive rate by 28.8-72.4% whereas difenoconazole did it from 12.2% to complete suppression. Neither of them affected the fertility of C. elegans. These findings suggest that the alkaloids fraction of N. nigra leaves is less toxic than difenoconazole and is a promising botanical fungicide against pathogenic soybean fungi.EEA ParanáFil: Sequin, Christian J. Universidad Nacional de Entre Ríos. Facultad de Agronomía; ArgentinaFil: Appelhans, Estefania Carolina. Universidad Nacional de Entre Ríos. Facultad de Agronomía; ArgentinaFil: Appelhans, Estefania Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; ArgentinaFil: Heis, Magalí S. Universidad Nacional de Entre Ríos. Facultad de Agronomía; ArgentinaFil: Torrent, Walter A. Universidad Nacional de Entre Ríos. Facultad de Agronomía; ArgentinaFil: Trossero, Julia A. Universidad Nacional de Entre Ríos. Facultad de Agronomía; ArgentinaFil: Catalan, Cesar Atilio Nazareno. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. LABIFITO; ArgentinaFil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. LABIFITO; ArgentinaFil: Aceñolaza, Pablo Gilberto. Universidad Nacional de Entre Ríos. Facultad de Agronomía; ArgentinaElsevier2023-11-13T14:12:25Z2023-11-13T14:12:25Z2023-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15939https://www.sciencedirect.com/science/article/pii/S18788181230031581878-8181https://doi.org/10.1016/j.bcab.2023.102914Biocatalysis and Agricultural Biotechnology 54 : 102914. (November 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:12Zoai:localhost:20.500.12123/15939instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:13.235INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
title Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
spellingShingle Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
Sequin, Christian J.
Antifungal Plants
Antifungal Properties
Alkaloids
Toxicology
Propiedades Antimicosicas
Alcaloides
Fabaceae
Toxicología
Neltuma nigra
title_short Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
title_full Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
title_fullStr Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
title_full_unstemmed Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
title_sort Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
dc.creator.none.fl_str_mv Sequin, Christian J.
Appelhans, Stefania Carolina
Heis, Magalí S.
Torrent, Walter A.
Trossero, Julia A.
Catalan, Cesar Atilio Nazareno
Sampietro, Diego Alejandro
Aceñolaza, Pablo Gilberto
author Sequin, Christian J.
author_facet Sequin, Christian J.
Appelhans, Stefania Carolina
Heis, Magalí S.
Torrent, Walter A.
Trossero, Julia A.
Catalan, Cesar Atilio Nazareno
Sampietro, Diego Alejandro
Aceñolaza, Pablo Gilberto
author_role author
author2 Appelhans, Stefania Carolina
Heis, Magalí S.
Torrent, Walter A.
Trossero, Julia A.
Catalan, Cesar Atilio Nazareno
Sampietro, Diego Alejandro
Aceñolaza, Pablo Gilberto
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Antifungal Plants
Antifungal Properties
Alkaloids
Toxicology
Propiedades Antimicosicas
Alcaloides
Fabaceae
Toxicología
Neltuma nigra
topic Antifungal Plants
Antifungal Properties
Alkaloids
Toxicology
Propiedades Antimicosicas
Alcaloides
Fabaceae
Toxicología
Neltuma nigra
dc.description.none.fl_txt_mv The use of synthetic fungicides for control soybean crop diseases has been cuestionated because of its impacts on human health and the environment. This work evaluated the composition, antifungal activity and toxicity of the alkaloids fraction obtained from Neltuma nigra leaves and compared its effect with the fungicide difenoconazole on Sclerotium rolfsii, Cercospora kikuchii, and C. sojina. Alkaloids fraction was extracted from leaves of N. nigra and its composition was analyzed by gas-chromatography coupled to mass spectrometry (GC-MS). The antifungal effect was recorded by measuring diameters of halos of inhibition of the growth of the different evaluated fungis. The toxicity of the alkaloids fraction was carried out using the method of Caenorhabditis elegans toxicity test. The extracted alkaloids fraction contained the alkaloids tryptamine (63.9%), tetrahydroxyharman (13.8%), phenethylamine (11.1%), cassine (10.4%), and N-formylphenylethylamine (0.8%). The alkaloids fraction was as effective against S. rolfsii as on the Cercospora strains, with minimum inhibitory doses of 25.0 μg disc-1 and inhibitory halos in the range of 8.5-13.0 mm, while difenoconazole had no effect on S. rolfsii. The toxicity of the alkaloid fraction (30.4-68.8 mg L-1) and difenoconazole (0.1-2.8 mg L-1) was tested on the reproduction, body length, and fertility of C. elegans. The fraction reduced the reproductive rate by 28.8-72.4% whereas difenoconazole did it from 12.2% to complete suppression. Neither of them affected the fertility of C. elegans. These findings suggest that the alkaloids fraction of N. nigra leaves is less toxic than difenoconazole and is a promising botanical fungicide against pathogenic soybean fungi.
EEA Paraná
Fil: Sequin, Christian J. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Appelhans, Estefania Carolina. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Appelhans, Estefania Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina
Fil: Heis, Magalí S. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Torrent, Walter A. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Trossero, Julia A. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
Fil: Catalan, Cesar Atilio Nazareno. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. LABIFITO; Argentina
Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. LABIFITO; Argentina
Fil: Aceñolaza, Pablo Gilberto. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina
description The use of synthetic fungicides for control soybean crop diseases has been cuestionated because of its impacts on human health and the environment. This work evaluated the composition, antifungal activity and toxicity of the alkaloids fraction obtained from Neltuma nigra leaves and compared its effect with the fungicide difenoconazole on Sclerotium rolfsii, Cercospora kikuchii, and C. sojina. Alkaloids fraction was extracted from leaves of N. nigra and its composition was analyzed by gas-chromatography coupled to mass spectrometry (GC-MS). The antifungal effect was recorded by measuring diameters of halos of inhibition of the growth of the different evaluated fungis. The toxicity of the alkaloids fraction was carried out using the method of Caenorhabditis elegans toxicity test. The extracted alkaloids fraction contained the alkaloids tryptamine (63.9%), tetrahydroxyharman (13.8%), phenethylamine (11.1%), cassine (10.4%), and N-formylphenylethylamine (0.8%). The alkaloids fraction was as effective against S. rolfsii as on the Cercospora strains, with minimum inhibitory doses of 25.0 μg disc-1 and inhibitory halos in the range of 8.5-13.0 mm, while difenoconazole had no effect on S. rolfsii. The toxicity of the alkaloid fraction (30.4-68.8 mg L-1) and difenoconazole (0.1-2.8 mg L-1) was tested on the reproduction, body length, and fertility of C. elegans. The fraction reduced the reproductive rate by 28.8-72.4% whereas difenoconazole did it from 12.2% to complete suppression. Neither of them affected the fertility of C. elegans. These findings suggest that the alkaloids fraction of N. nigra leaves is less toxic than difenoconazole and is a promising botanical fungicide against pathogenic soybean fungi.
publishDate 2023
dc.date.none.fl_str_mv 2023-11-13T14:12:25Z
2023-11-13T14:12:25Z
2023-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15939
https://www.sciencedirect.com/science/article/pii/S1878818123003158
1878-8181
https://doi.org/10.1016/j.bcab.2023.102914
url http://hdl.handle.net/20.500.12123/15939
https://www.sciencedirect.com/science/article/pii/S1878818123003158
https://doi.org/10.1016/j.bcab.2023.102914
identifier_str_mv 1878-8181
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Biocatalysis and Agricultural Biotechnology 54 : 102914. (November 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619181840924672
score 12.559606

Publicaciones similares