Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia

Autores
Matsusaka, Daniel; Filiault, Daniele; Sánchez, Diego H.; Botto, Javier Francisco
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Matsusaka, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Matsusaka, Daniel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Filiault, Daniele. Austrian Academy of Sciences. Gregor Mendel Institute. Vienna BioCenter. Vienna, Austria.
Fil: Sánchez, Diego H. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Sánchez, Diego H. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Botto, Javier Francisco. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Botto, Javier Francisco. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Arabidopsis thaliana shows a wide range of genetic and trait variation among wild accessions. Because of its unparalleled biological and genomic resources, Arabidopsis has a high potential for the identification of genes underlying ecologically important complex traits, thus providing new insights on genome evolution. Previous research suggested that distinct light responses were crucial for Arabidopsis establishment in a peculiar ecological niche of southern Patagonia. The aim of this study was to explore the genetic basis of contrasting light-associated physiological traits that may have mediated the rapid adaptation to this new environment. From a biparental cross between the photomorphogenic contrasting accessions Patagonia (Pat) and Columbia (Col-0), we generated a novel recombinant inbred line (RIL) population, which was entirely next-generation sequenced to achieve ultra-high-density saturating molecular markers resulting in supreme mapping sensitivity. We validated the quality of the RIL population by quantitative trait loci (QTL) mapping for seedling de-etiolation, finding seven QTLs for hypocotyl length in the dark and continuous blue light (Bc), continuous red light (Rc), and continuous far-red light (FRc). The most relevant QTLs, Rc1 and Bc1, were mapped close together to chromosome V; the former for Rc and Rc/dark, and the latter for Bc, FRc, and dark treatments. The additive effects of both QTLs were confirmed by independent heterogeneous inbred families (HIFs), and we explored TZP and ABA1 as potential candidate genes for Rc1 and Bc1QTLs, respectively. We conclude that the Pat × Col-0 RIL population is a valuable novel genetic resource to explore other adaptive traits in Arabidopsis.
grafs.
Fuente
Frontiers in Plant Science
Vol.12
e677728
http://www.frontiersin.org
Materia
ARABIDOPSIS
RILS
HIFS
QTL
SEEDLING DE ETIOLATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2021matsusaka

id FAUBA_e912db0b592bf293329dfdd625123b41
oai_identifier_str snrd:2021matsusaka
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern PatagoniaMatsusaka, DanielFiliault, DanieleSánchez, Diego H.Botto, Javier FranciscoARABIDOPSISRILSHIFSQTLSEEDLING DE ETIOLATIONFil: Matsusaka, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.Fil: Matsusaka, Daniel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.Fil: Filiault, Daniele. Austrian Academy of Sciences. Gregor Mendel Institute. Vienna BioCenter. Vienna, Austria.Fil: Sánchez, Diego H. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.Fil: Sánchez, Diego H. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.Fil: Botto, Javier Francisco. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.Fil: Botto, Javier Francisco. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.Arabidopsis thaliana shows a wide range of genetic and trait variation among wild accessions. Because of its unparalleled biological and genomic resources, Arabidopsis has a high potential for the identification of genes underlying ecologically important complex traits, thus providing new insights on genome evolution. Previous research suggested that distinct light responses were crucial for Arabidopsis establishment in a peculiar ecological niche of southern Patagonia. The aim of this study was to explore the genetic basis of contrasting light-associated physiological traits that may have mediated the rapid adaptation to this new environment. From a biparental cross between the photomorphogenic contrasting accessions Patagonia (Pat) and Columbia (Col-0), we generated a novel recombinant inbred line (RIL) population, which was entirely next-generation sequenced to achieve ultra-high-density saturating molecular markers resulting in supreme mapping sensitivity. We validated the quality of the RIL population by quantitative trait loci (QTL) mapping for seedling de-etiolation, finding seven QTLs for hypocotyl length in the dark and continuous blue light (Bc), continuous red light (Rc), and continuous far-red light (FRc). The most relevant QTLs, Rc1 and Bc1, were mapped close together to chromosome V; the former for Rc and Rc/dark, and the latter for Bc, FRc, and dark treatments. The additive effects of both QTLs were confirmed by independent heterogeneous inbred families (HIFs), and we explored TZP and ABA1 as potential candidate genes for Rc1 and Bc1QTLs, respectively. We conclude that the Pat × Col-0 RIL population is a valuable novel genetic resource to explore other adaptive traits in Arabidopsis.grafs.2021articleinfo:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.3389/fpls.2021.677728issn:1664-462Xhttp://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021matsusakaFrontiers in Plant ScienceVol.12e677728http://www.frontiersin.orgreponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaeng7016766Patagonia (general region)info:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-29T13:41:05Zsnrd:2021matsusakainstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-29 13:41:05.757FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia
title Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia
spellingShingle Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia
Matsusaka, Daniel
ARABIDOPSIS
RILS
HIFS
QTL
SEEDLING DE ETIOLATION
title_short Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia
title_full Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia
title_fullStr Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia
title_full_unstemmed Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia
title_sort Ultra - high - density QTL marker mapping for seedling photomorphogenesis mediating Arabidopsis establishment in Southern Patagonia
dc.creator.none.fl_str_mv Matsusaka, Daniel
Filiault, Daniele
Sánchez, Diego H.
Botto, Javier Francisco
author Matsusaka, Daniel
author_facet Matsusaka, Daniel
Filiault, Daniele
Sánchez, Diego H.
Botto, Javier Francisco
author_role author
author2 Filiault, Daniele
Sánchez, Diego H.
Botto, Javier Francisco
author2_role author
author
author
dc.subject.none.fl_str_mv ARABIDOPSIS
RILS
HIFS
QTL
SEEDLING DE ETIOLATION
topic ARABIDOPSIS
RILS
HIFS
QTL
SEEDLING DE ETIOLATION
dc.description.none.fl_txt_mv Fil: Matsusaka, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Matsusaka, Daniel. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Filiault, Daniele. Austrian Academy of Sciences. Gregor Mendel Institute. Vienna BioCenter. Vienna, Austria.
Fil: Sánchez, Diego H. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Sánchez, Diego H. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Botto, Javier Francisco. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Fil: Botto, Javier Francisco. CONICET – Universidad de Buenos Aires. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
Arabidopsis thaliana shows a wide range of genetic and trait variation among wild accessions. Because of its unparalleled biological and genomic resources, Arabidopsis has a high potential for the identification of genes underlying ecologically important complex traits, thus providing new insights on genome evolution. Previous research suggested that distinct light responses were crucial for Arabidopsis establishment in a peculiar ecological niche of southern Patagonia. The aim of this study was to explore the genetic basis of contrasting light-associated physiological traits that may have mediated the rapid adaptation to this new environment. From a biparental cross between the photomorphogenic contrasting accessions Patagonia (Pat) and Columbia (Col-0), we generated a novel recombinant inbred line (RIL) population, which was entirely next-generation sequenced to achieve ultra-high-density saturating molecular markers resulting in supreme mapping sensitivity. We validated the quality of the RIL population by quantitative trait loci (QTL) mapping for seedling de-etiolation, finding seven QTLs for hypocotyl length in the dark and continuous blue light (Bc), continuous red light (Rc), and continuous far-red light (FRc). The most relevant QTLs, Rc1 and Bc1, were mapped close together to chromosome V; the former for Rc and Rc/dark, and the latter for Bc, FRc, and dark treatments. The additive effects of both QTLs were confirmed by independent heterogeneous inbred families (HIFs), and we explored TZP and ABA1 as potential candidate genes for Rc1 and Bc1QTLs, respectively. We conclude that the Pat × Col-0 RIL population is a valuable novel genetic resource to explore other adaptive traits in Arabidopsis.
grafs.
description Fil: Matsusaka, Daniel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA). Buenos Aires, Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv article
info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv doi:10.3389/fpls.2021.677728
issn:1664-462X
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021matsusaka
identifier_str_mv doi:10.3389/fpls.2021.677728
issn:1664-462X
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2021matsusaka
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv 7016766
Patagonia (general region)
dc.source.none.fl_str_mv Frontiers in Plant Science
Vol.12
e677728
http://www.frontiersin.org
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
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