Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis

Autores
Krzymuski, Martin Javier; Cerdan, Pablo Diego; Zhu, Ling; Vinh, Amanda; Chory, Joanne; Huq, Enamul; Casal, Jorge José
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phytochrome A (phyA) is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light (typical of dense vegetation canopies). However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wildtype(WT), hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 (PIF1) with those genes showing hyperpromotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development.
Fil: Krzymuski, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Cerdan, Pablo Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Zhu, Ling. University of Texas at Austin; Estados Unidos
Fil: Vinh, Amanda. University of Texas at Austin; Estados Unidos
Fil: Chory, Joanne. Salk Institute. Plant Biology Laboratory; Estados Unidos
Fil: Huq, Enamul. University of Texas at Austin; Estados Unidos
Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Materia
PHYA
PIF1
DE-ETIOLATION
LIGHT (GENE EXPRESSION)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/102872
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/102872
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of PhotomorphogenesisKrzymuski, Martin JavierCerdan, Pablo DiegoZhu, LingVinh, AmandaChory, JoanneHuq, EnamulCasal, Jorge JoséPHYAPIF1DE-ETIOLATIONLIGHT (GENE EXPRESSION)https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phytochrome A (phyA) is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light (typical of dense vegetation canopies). However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wildtype(WT), hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 (PIF1) with those genes showing hyperpromotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development.Fil: Krzymuski, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Cerdan, Pablo Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Zhu, Ling. University of Texas at Austin; Estados UnidosFil: Vinh, Amanda. University of Texas at Austin; Estados UnidosFil: Chory, Joanne. Salk Institute. Plant Biology Laboratory; Estados UnidosFil: Huq, Enamul. University of Texas at Austin; Estados UnidosFil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaOxford University Press2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/102872Krzymuski, Martin Javier; Cerdan, Pablo Diego; Zhu, Ling; Vinh, Amanda; Chory, Joanne; et al.; Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis; Oxford University Press; Molecular Plant; 7; 9; 9-2014; 1415-14281674-2052CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/mp/ssu078info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/molecular-plant/fulltext/S1674-2052(14)60945-X?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS167420521460945X%3Fshowall%3Dtrueinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:25Zoai:ri.conicet.gov.ar:11336/102872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:25.939CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis
title Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis
spellingShingle Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis
Krzymuski, Martin Javier
PHYA
PIF1
DE-ETIOLATION
LIGHT (GENE EXPRESSION)
title_short Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis
title_full Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis
title_fullStr Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis
title_full_unstemmed Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis
title_sort Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis
dc.creator.none.fl_str_mv Krzymuski, Martin Javier
Cerdan, Pablo Diego
Zhu, Ling
Vinh, Amanda
Chory, Joanne
Huq, Enamul
Casal, Jorge José
author Krzymuski, Martin Javier
author_facet Krzymuski, Martin Javier
Cerdan, Pablo Diego
Zhu, Ling
Vinh, Amanda
Chory, Joanne
Huq, Enamul
Casal, Jorge José
author_role author
author2 Cerdan, Pablo Diego
Zhu, Ling
Vinh, Amanda
Chory, Joanne
Huq, Enamul
Casal, Jorge José
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv PHYA
PIF1
DE-ETIOLATION
LIGHT (GENE EXPRESSION)
topic PHYA
PIF1
DE-ETIOLATION
LIGHT (GENE EXPRESSION)
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phytochrome A (phyA) is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light (typical of dense vegetation canopies). However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wildtype(WT), hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 (PIF1) with those genes showing hyperpromotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development.
Fil: Krzymuski, Martin Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Cerdan, Pablo Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Zhu, Ling. University of Texas at Austin; Estados Unidos
Fil: Vinh, Amanda. University of Texas at Austin; Estados Unidos
Fil: Chory, Joanne. Salk Institute. Plant Biology Laboratory; Estados Unidos
Fil: Huq, Enamul. University of Texas at Austin; Estados Unidos
Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
description Phytochrome A (phyA) is crucial to initiate the early steps of the transition between skoto- and photomorphogenesis upon light exposure and to complete this process under far-red light (typical of dense vegetation canopies). However, under prolonged red or white light, phyA mutants are hyper-photomorphogenic in many respects. To investigate this issue, we analyzed the late response of the transcriptome of the phyA mutant to red light. Compared to the wildtype(WT), hyper-responsive genes outnumbered the genes showing reduced response to red light in phyA. A network analysis revealed the co-expression of PHYTOCHROME INTERACTING FACTOR 1 (PIF1) with those genes showing hyperpromotion by red light in phyA. The enhanced responses of gene expression, cotyledon unfolding, hypocotyl growth, and greening observed in the phyA mutant compared to the WT were absent in the phyA pif1 double mutant compared to pif1, indicating that the hyper-photomorphogenic phenotype of phyA requires PIF1. PIF1 directly binds to gene promoters that displayed PIF1-mediated enhanced response to red light. Expression of mutant PIF1 deficient in interactions with phyA and phyB enhanced the long-term growth response to red light but reduced the expression of selected genes in response to red light. We propose that phytochrome-mediated degradation of PIF1 prevents over-activation of photomorphogenesis during early seedling development.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/102872
Krzymuski, Martin Javier; Cerdan, Pablo Diego; Zhu, Ling; Vinh, Amanda; Chory, Joanne; et al.; Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis; Oxford University Press; Molecular Plant; 7; 9; 9-2014; 1415-1428
1674-2052
CONICET Digital
CONICET
url http://hdl.handle.net/11336/102872
identifier_str_mv Krzymuski, Martin Javier; Cerdan, Pablo Diego; Zhu, Ling; Vinh, Amanda; Chory, Joanne; et al.; Phytochrome A Antagonizes PHYTOCHROME INTERACTING FACTOR 1 to Prevent Over-Activation of Photomorphogenesis; Oxford University Press; Molecular Plant; 7; 9; 9-2014; 1415-1428
1674-2052
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/mp/ssu078
info:eu-repo/semantics/altIdentifier/url/https://www.cell.com/molecular-plant/fulltext/S1674-2052(14)60945-X?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS167420521460945X%3Fshowall%3Dtrue
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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