Impact of infection dose and previous serum antibodies against the locus of enterocyte effacement proteins on Escherichia coli O157:H7 shedding in calves following experimental Inf...

• Autores: Martorelli, L.; Hovde, C. J.; Vilte, Daniel A.; Albanese, A.; Zotta, E.; Ibarra, C.; Cantet, Rodolfo Juan Carlos; Mercado, Elsa C.; Cataldi, Angel Adrián
• Año de publicación: 2015
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: BioMed Research International; Vol.2015; Article ID 290679; http://www.hindawi.com
• Materia: ESCHERICHIA COLI; HAEMOLYTIC UREMIC SYNDROME; BACTERIA
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Martorelli, L. Instituto de Patobiología, CICVyA, INTA, Hurlingham, Argentina.
Fil: Hovde, C. J. School of Food Science, University of Idaho, Moscow, USA.
Fil: Vilte, Daniel A. Instituto de Patobiología, CICVyA, INTA, Hurlingham, Argentina.
Fil: Albanese, A. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires, Argentina.
Fil: Zotta, E. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires, Argentina.
Fil: Ibarra, C. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología. Laboratorio de Fisiopatogenia. Buenos Aires, Argentina.
Fil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomía. Buenos Aires, Argentina.
Fil: Cantet, Rodolfo Juan Carlos. CONICET. Buenos Aires, Argentina.
Fil: Mercado, Elsa C. Instituto de Patobiología, CICVyA, INTA, Hurlingham, Argentina.
Fil: Cataldi, Angel Adrián. Instituto de Biotecnología, CICVyA, INTA, Hurlingham, Argentina.
Escherichia coli O157:H7 is themain causative agent of haemolytic uremic syndrome. Cattle are themain reservoir of these bacteria, and have been shown to develop immune response to colonization. Our aim was to investigate the faecal shedding pattern of E. coli O157:H7 in calves challenged intragastrically with either 108 or 1010 CFU, as well as the ability of specific preexisting antibodies to reduce shedding of the pathogen. Shedding was analysed by direct counting as well as enrichment of rectoanal mucosal swabs. Statistical analysis was performed using a linear model for repeated measures with and without the inclusion of preexisting antibodies against the carboxy - terminal fraction of intimin-. (.-intimin C280) as a covariable. Results suggest that there is a statistical difference in the area under the shedding curves between both doses for 14 as well as 28 days after challenge (p = 0.0069 and 0.0209, resp.). This difference is increased when the prechallenge antibodies are taken into account (p = 0.0056 and 0.0185). We concluded that the bacterial dose influences shedding on calves experimentally challenged and that preexisting antibodies against E. coli O157:H7 .-intimin C280 could partially reduce faecal excretion.