Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental inf...

Autores
Martorelli, Luisina; Hovde, C. J.; Vilte, D. A.; Albanese, A.; Zotta, E.; Ibarra, Cristina Adriana; Cantet, Rodolfo Juan Carlos; Mercado, E. C.; Cataldi, Angel Adrian
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Escherichia coli O157:H7 is the main causative agent of haemolytic uremic syndrome. Cattle are the main reservoir of these bacteria, and have been shown to develop immune response to colonization. Our aim was to investigate the faecal shedding pattern of E. coli O157:H7 in calves challenged intragastrically with either 108 or 1010 CFU, as well as the ability of specific preexisting antibodies to reduce shedding of the pathogen. Shedding was analysed by direct counting as well as enrichment of rectoanal mucosal swabs. Statistical analysis was performed using a linear model for repeated measures with and without the inclusion of preexisting antibodies against the carboxy-terminal fraction of intimin- (-intimin C280) as a covariable. Results suggest that there is a statistical difference in the area under the shedding curves between both doses for 14 as well as 28 days after challenge (p = 0.0069 and 0.0209, resp.). This difference is increased when the prechallenge antibodies are taken into account (p = 0.0056 and 0.0185). We concluded that the bacterial dose influences shedding on calves experimentally challenged and that preexisting antibodies against E. coli O157:H7 -intimin C280 could partially reduce faecal excretion.
Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hovde, C. J.. University Of Idaho; Estados Unidos
Fil: Vilte, D. A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Albanese, A.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Zotta, E.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mercado, E. C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
E. coli O157:H7
Calves
Experimental infection
Antibodies anti-LEE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/16763
Acceder
id CONICETDig_8a698e358ba5d33eb463b438053fb610
oai_identifier_str oai:ri.conicet.gov.ar:11336/16763
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infectionMartorelli, LuisinaHovde, C. J.Vilte, D. A.Albanese, A.Zotta, E.Ibarra, Cristina AdrianaCantet, Rodolfo Juan CarlosMercado, E. C.Cataldi, Angel AdrianE. coli O157:H7CalvesExperimental infectionAntibodies anti-LEEhttps://purl.org/becyt/ford/3.4https://purl.org/becyt/ford/3Escherichia coli O157:H7 is the main causative agent of haemolytic uremic syndrome. Cattle are the main reservoir of these bacteria, and have been shown to develop immune response to colonization. Our aim was to investigate the faecal shedding pattern of E. coli O157:H7 in calves challenged intragastrically with either 108 or 1010 CFU, as well as the ability of specific preexisting antibodies to reduce shedding of the pathogen. Shedding was analysed by direct counting as well as enrichment of rectoanal mucosal swabs. Statistical analysis was performed using a linear model for repeated measures with and without the inclusion of preexisting antibodies against the carboxy-terminal fraction of intimin- (-intimin C280) as a covariable. Results suggest that there is a statistical difference in the area under the shedding curves between both doses for 14 as well as 28 days after challenge (p = 0.0069 and 0.0209, resp.). This difference is increased when the prechallenge antibodies are taken into account (p = 0.0056 and 0.0185). We concluded that the bacterial dose influences shedding on calves experimentally challenged and that preexisting antibodies against E. coli O157:H7 -intimin C280 could partially reduce faecal excretion.Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hovde, C. J.. University Of Idaho; Estados UnidosFil: Vilte, D. A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Albanese, A.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; ArgentinaFil: Zotta, E.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; ArgentinaFil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mercado, E. C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaHindawi Publishing Corporation2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/16763Martorelli, Luisina; Hovde, C. J.; Vilte, D. A.; Albanese, A.; Zotta, E.; et al.; Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection; Hindawi Publishing Corporation; BioMed Research International; 2015; -1-2015; 1-8; 2906792314-61332314-6141enginfo:eu-repo/semantics/altIdentifier/doi/10.1155/2015/290679info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2015/290679/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:35:57Zoai:ri.conicet.gov.ar:11336/16763instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:35:57.996CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
title Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
spellingShingle Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
Martorelli, Luisina
E. coli O157:H7
Calves
Experimental infection
Antibodies anti-LEE
title_short Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
title_full Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
title_fullStr Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
title_full_unstemmed Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
title_sort Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection
dc.creator.none.fl_str_mv Martorelli, Luisina
Hovde, C. J.
Vilte, D. A.
Albanese, A.
Zotta, E.
Ibarra, Cristina Adriana
Cantet, Rodolfo Juan Carlos
Mercado, E. C.
Cataldi, Angel Adrian
author Martorelli, Luisina
author_facet Martorelli, Luisina
Hovde, C. J.
Vilte, D. A.
Albanese, A.
Zotta, E.
Ibarra, Cristina Adriana
Cantet, Rodolfo Juan Carlos
Mercado, E. C.
Cataldi, Angel Adrian
author_role author
author2 Hovde, C. J.
Vilte, D. A.
Albanese, A.
Zotta, E.
Ibarra, Cristina Adriana
Cantet, Rodolfo Juan Carlos
Mercado, E. C.
Cataldi, Angel Adrian
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv E. coli O157:H7
Calves
Experimental infection
Antibodies anti-LEE
topic E. coli O157:H7
Calves
Experimental infection
Antibodies anti-LEE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.4
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Escherichia coli O157:H7 is the main causative agent of haemolytic uremic syndrome. Cattle are the main reservoir of these bacteria, and have been shown to develop immune response to colonization. Our aim was to investigate the faecal shedding pattern of E. coli O157:H7 in calves challenged intragastrically with either 108 or 1010 CFU, as well as the ability of specific preexisting antibodies to reduce shedding of the pathogen. Shedding was analysed by direct counting as well as enrichment of rectoanal mucosal swabs. Statistical analysis was performed using a linear model for repeated measures with and without the inclusion of preexisting antibodies against the carboxy-terminal fraction of intimin- (-intimin C280) as a covariable. Results suggest that there is a statistical difference in the area under the shedding curves between both doses for 14 as well as 28 days after challenge (p = 0.0069 and 0.0209, resp.). This difference is increased when the prechallenge antibodies are taken into account (p = 0.0056 and 0.0185). We concluded that the bacterial dose influences shedding on calves experimentally challenged and that preexisting antibodies against E. coli O157:H7 -intimin C280 could partially reduce faecal excretion.
Fil: Martorelli, Luisina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hovde, C. J.. University Of Idaho; Estados Unidos
Fil: Vilte, D. A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Albanese, A.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Zotta, E.. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina
Fil: Ibarra, Cristina Adriana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Ciencias Fisiológicas. Laboratorio de Fisiopatogenia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cantet, Rodolfo Juan Carlos. Universidad de Buenos Aires. Facultad de Agronomia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mercado, E. C.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Escherichia coli O157:H7 is the main causative agent of haemolytic uremic syndrome. Cattle are the main reservoir of these bacteria, and have been shown to develop immune response to colonization. Our aim was to investigate the faecal shedding pattern of E. coli O157:H7 in calves challenged intragastrically with either 108 or 1010 CFU, as well as the ability of specific preexisting antibodies to reduce shedding of the pathogen. Shedding was analysed by direct counting as well as enrichment of rectoanal mucosal swabs. Statistical analysis was performed using a linear model for repeated measures with and without the inclusion of preexisting antibodies against the carboxy-terminal fraction of intimin- (-intimin C280) as a covariable. Results suggest that there is a statistical difference in the area under the shedding curves between both doses for 14 as well as 28 days after challenge (p = 0.0069 and 0.0209, resp.). This difference is increased when the prechallenge antibodies are taken into account (p = 0.0056 and 0.0185). We concluded that the bacterial dose influences shedding on calves experimentally challenged and that preexisting antibodies against E. coli O157:H7 -intimin C280 could partially reduce faecal excretion.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/16763
Martorelli, Luisina; Hovde, C. J.; Vilte, D. A.; Albanese, A.; Zotta, E.; et al.; Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection; Hindawi Publishing Corporation; BioMed Research International; 2015; -1-2015; 1-8; 290679
2314-6133
2314-6141
url http://hdl.handle.net/11336/16763
identifier_str_mv Martorelli, Luisina; Hovde, C. J.; Vilte, D. A.; Albanese, A.; Zotta, E.; et al.; Impact of infection dose and previous serum antibodies against the Locus of Enterocyte effacement proteins on Escherichia Coli O157:H7 shedding in calves following experimental infection; Hindawi Publishing Corporation; BioMed Research International; 2015; -1-2015; 1-8; 290679
2314-6133
2314-6141
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1155/2015/290679
info:eu-repo/semantics/altIdentifier/url/https://www.hindawi.com/journals/bmri/2015/290679/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.216834

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