Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis

• Autores: Blanco, Federico Carlos; Soria, Marcelo Abel; Bianco, María Verónica; Bigi, Fabiana
• Año de publicación: 2012
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Plos One; Vol.7, no.7; e41066; http://www.plos.org/
• Materia: AMPHIREGULIN B; BIOLOGICAL MARKER; CALGRANULIN B; CD14 ANTIGEN; CHEMOKINE RECEPTOR CXCR2; CXCL9 CHEMOKINE; FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR; GELATINASE B; HYALURONAN SYNTHASE 2; INTERLEUKIN 1 RECEPTOR; INTERLEUKIN 13 RECEPTOR ALPHA1; INTERLEUKIN 22; LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN; MANNOSE RECEPTOR C TYPE 1; MATRIX METALLOPROTEINASE 19; MONOCYTE CHEMOTACTIC PROTEIN 2; NEUROPILIN 1; RESISTIN; RNA; SH3 DOMAIN BINDING PROTEIN 4; THROMBOCYTE FACTOR 4; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR FYVE; TRANSCRIPTION FACTOR GZMA; TRANSCRIPTION FACTOR THBS1; TRANSFORMING GROWTH FACTOR BETA; TRANSGLUTAMINASE 3; UNCLASSIFIED DRUG; UROKINASE RECEPTOR; VERY LOW DENSITY LIPOPROTEIN RECEPTOR; ADAM8 GENE; ADAMTSL4 GENE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; APOPTOSIS; AREGB GENE; ARHGAP21 GENE; BOVINE TUBERCULOSIS; CATTLE DISEASE; CCL2 GENE; CCL8 GENE; CCR1 GENE; CD14 GENE; CELL TRANSPORT; CSF3R GENE; CXCL10 GENE; CXCL9 GENE; CXCR2 GENE; DEFBIO GENE; DOWN REGULATION; ECM1 GENE; ENDOCYTOSIS; FCAR GENE; FCGR1A GENE; FYVE GENE; GENE; GENE EXPRESSION PROFILING; GENETIC VARIABILITY; GZMA GENE; HAS2 GENE; IL 18 GENE; IL 1R GENE; IL 6 GENE; IL13RA1 GENE; IL1R1 GENE; IL1RN GENE; IL22 GENE; IMMUNE RESPONSE; INFLAMMATION; LOC508666 GENE; LOC533894 GENE; MAPK13 GENE; MAPK14 GENE; MICROARRAY ANALYSIS; MMP19 GENE; MMP3 GENE; MMP9 GENE; MRC1 GENE; MYCOBACTERIUM BOVIS; MYD88 GENE; NFAM1 GENE; NONHUMAN; NRP1 GENE; PERIPHERAL BLOOD MONONUCLEAR CELL; PF4 GENE; PLAUR GENE; PROTEIN DEGRADATION; PYCARD GENE; QUANTITATIVE ANALYSIS; RETN GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; S100A9 GENE; SASH1 GENE; SH3BP4 GENE; TGFBI GENE; TGM3 GENE; THBS1 GENE; TREM1 GENE; TYROBP GENE; UPREGULATION; VLDLR GENE; ANIMALS; BIOLOGICAL MARKERS; CATTLE; ENDOCYTOSIS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, BACTERIAL; INFLAMMATION; LEUKOCYTES, MONONUCLEAR; MODELS, GENETIC; MYCOBACTERIUM BOVIS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; TRANSCRIPTION, GENETIC; TUBERCULOSIS, BOVINE; BOS; BOVINAE; MYCOBACTERIUM BOVIS
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVyA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. CONICET - Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA). uenos Aires, Argentina.
Fil: Bianco, María Verónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVyA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas (CICVyA). Instituto de Biotecnología. Hurlingham, Buenos Aires, Argentina.
Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis.