Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts

Autores
Isaac, Paula; Lozada, Mariana; Dionisi, Hebe Monica; Estévez, Maria Cristina; Ferrero, Marcela Alejandra
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A combination of RT-PCR assays and of Northern blots were used to evaluate the ability of naphthalene, phenanthrene and pyrene to induce nahAc from three Pseudomonas isolates obtained from oil-contaminated marine sediments. Naphthalene dioxygenase activity based on indigo oxidation correlated with nahAc expression in all strains, while variable polycyclic aromatic hydrocarbon (PAH) degradation behaviors were observed. Naphthalene was completely degraded by all strains; however, whereas high levels of nahAc transcripts were detected in cultures of Pseudomonas monteilii P26 and Pseudomonas stutzeri N3 grown with naphthalene, significantly lower levels were detected in those of Pseudomonas xanthomarina N12. Phenanthrene was degraded by strain P6 and it strongly induced nahAc in this strain. On the other hand, although the strains N12 and N3 removed phenanthrene, the levels of nahAc transcripts were very low when these two strains were grown with this substrate. Remarkably, when P. stutzeri N3 was exposed to pyrene, an intense band of nahAc transcripts was detected by RT-PCR and Northern blot, even though pyrene was degraded to less than 5%. Our results indicate that all the strains from coastal Patagonia displayed the potential to biodegrade PAHs, although in some cases there was no clear correlation between nahAc gene expression and the ability to degrade PAHs.
Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Estévez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Materia
Pah
Catabolic Gene
Gene Expression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5589
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/5589
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coastsIsaac, PaulaLozada, MarianaDionisi, Hebe MonicaEstévez, Maria CristinaFerrero, Marcela AlejandraPahCatabolic GeneGene Expressionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1A combination of RT-PCR assays and of Northern blots were used to evaluate the ability of naphthalene, phenanthrene and pyrene to induce nahAc from three Pseudomonas isolates obtained from oil-contaminated marine sediments. Naphthalene dioxygenase activity based on indigo oxidation correlated with nahAc expression in all strains, while variable polycyclic aromatic hydrocarbon (PAH) degradation behaviors were observed. Naphthalene was completely degraded by all strains; however, whereas high levels of nahAc transcripts were detected in cultures of Pseudomonas monteilii P26 and Pseudomonas stutzeri N3 grown with naphthalene, significantly lower levels were detected in those of Pseudomonas xanthomarina N12. Phenanthrene was degraded by strain P6 and it strongly induced nahAc in this strain. On the other hand, although the strains N12 and N3 removed phenanthrene, the levels of nahAc transcripts were very low when these two strains were grown with this substrate. Remarkably, when P. stutzeri N3 was exposed to pyrene, an intense band of nahAc transcripts was detected by RT-PCR and Northern blot, even though pyrene was degraded to less than 5%. Our results indicate that all the strains from coastal Patagonia displayed the potential to biodegrade PAHs, although in some cases there was no clear correlation between nahAc gene expression and the ability to degrade PAHs.Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; ArgentinaFil: Estévez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaElsevier2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5589Isaac, Paula; Lozada, Mariana; Dionisi, Hebe Monica; Estévez, Maria Cristina; Ferrero, Marcela Alejandra; Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts; Elsevier; International Biodeterioration And Biodegradation; 105; 8-2015; 1-60964-8305enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830515300664info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.08.011info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:39:44Zoai:ri.conicet.gov.ar:11336/5589instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:39:45.101CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts
title Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts
spellingShingle Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts
Isaac, Paula
Pah
Catabolic Gene
Gene Expression
title_short Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts
title_full Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts
title_fullStr Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts
title_full_unstemmed Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts
title_sort Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts
dc.creator.none.fl_str_mv Isaac, Paula
Lozada, Mariana
Dionisi, Hebe Monica
Estévez, Maria Cristina
Ferrero, Marcela Alejandra
author Isaac, Paula
author_facet Isaac, Paula
Lozada, Mariana
Dionisi, Hebe Monica
Estévez, Maria Cristina
Ferrero, Marcela Alejandra
author_role author
author2 Lozada, Mariana
Dionisi, Hebe Monica
Estévez, Maria Cristina
Ferrero, Marcela Alejandra
author2_role author
author
author
author
dc.subject.none.fl_str_mv Pah
Catabolic Gene
Gene Expression
topic Pah
Catabolic Gene
Gene Expression
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A combination of RT-PCR assays and of Northern blots were used to evaluate the ability of naphthalene, phenanthrene and pyrene to induce nahAc from three Pseudomonas isolates obtained from oil-contaminated marine sediments. Naphthalene dioxygenase activity based on indigo oxidation correlated with nahAc expression in all strains, while variable polycyclic aromatic hydrocarbon (PAH) degradation behaviors were observed. Naphthalene was completely degraded by all strains; however, whereas high levels of nahAc transcripts were detected in cultures of Pseudomonas monteilii P26 and Pseudomonas stutzeri N3 grown with naphthalene, significantly lower levels were detected in those of Pseudomonas xanthomarina N12. Phenanthrene was degraded by strain P6 and it strongly induced nahAc in this strain. On the other hand, although the strains N12 and N3 removed phenanthrene, the levels of nahAc transcripts were very low when these two strains were grown with this substrate. Remarkably, when P. stutzeri N3 was exposed to pyrene, an intense band of nahAc transcripts was detected by RT-PCR and Northern blot, even though pyrene was degraded to less than 5%. Our results indicate that all the strains from coastal Patagonia displayed the potential to biodegrade PAHs, although in some cases there was no clear correlation between nahAc gene expression and the ability to degrade PAHs.
Fil: Isaac, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Lozada, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Dionisi, Hebe Monica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagónico; Argentina
Fil: Estévez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Ferrero, Marcela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
description A combination of RT-PCR assays and of Northern blots were used to evaluate the ability of naphthalene, phenanthrene and pyrene to induce nahAc from three Pseudomonas isolates obtained from oil-contaminated marine sediments. Naphthalene dioxygenase activity based on indigo oxidation correlated with nahAc expression in all strains, while variable polycyclic aromatic hydrocarbon (PAH) degradation behaviors were observed. Naphthalene was completely degraded by all strains; however, whereas high levels of nahAc transcripts were detected in cultures of Pseudomonas monteilii P26 and Pseudomonas stutzeri N3 grown with naphthalene, significantly lower levels were detected in those of Pseudomonas xanthomarina N12. Phenanthrene was degraded by strain P6 and it strongly induced nahAc in this strain. On the other hand, although the strains N12 and N3 removed phenanthrene, the levels of nahAc transcripts were very low when these two strains were grown with this substrate. Remarkably, when P. stutzeri N3 was exposed to pyrene, an intense band of nahAc transcripts was detected by RT-PCR and Northern blot, even though pyrene was degraded to less than 5%. Our results indicate that all the strains from coastal Patagonia displayed the potential to biodegrade PAHs, although in some cases there was no clear correlation between nahAc gene expression and the ability to degrade PAHs.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5589
Isaac, Paula; Lozada, Mariana; Dionisi, Hebe Monica; Estévez, Maria Cristina; Ferrero, Marcela Alejandra; Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts; Elsevier; International Biodeterioration And Biodegradation; 105; 8-2015; 1-6
0964-8305
url http://hdl.handle.net/11336/5589
identifier_str_mv Isaac, Paula; Lozada, Mariana; Dionisi, Hebe Monica; Estévez, Maria Cristina; Ferrero, Marcela Alejandra; Differential expression of the catabolic nahAc gene and its effect on PAH degradation in Pseudomonas strains isolated from contaminated Patagonian coasts; Elsevier; International Biodeterioration And Biodegradation; 105; 8-2015; 1-6
0964-8305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830515300664
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2015.08.011
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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