Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots

Autores
Paris, Ramiro; Vazquez, María M.; Graziano, Magdalena; Terrile, Maria Cecilia; Miller, Nathan D.; Spalding, Edgar P.; Otegui, Marisa S.; Casalongue, Claudia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
High-resolution and automated image analysis of individual roots demonstrated that endogenous nitric oxide (NO) contribute significantly to gravitropism of Arabidopsis roots. Lowering of endogenous NO concentrations strongly reduced and even reversed gravitropism, resulting in upward bending, without affecting root growth rate. Notably, the asymmetric accumulation of NOalong the upper and lower sides of roots correlated with a positive gravitropic response. Detection of NO by the specific DAF-FM DA fluorescent probe revealed that NO was higher at the lower side of horizontally-oriented roots returning to initial values 2h after the onset of gravistimulation. We demonstrate that NO promotes plasma membrane re-localization of PIN2 in epidermal cells, which is required during the early root gravitropic response. The dynamic and asymmetric localization of both auxin and NO is critical to regulate auxin polar transport during gravitropism. Our results collectively suggest that, although auxin and NO crosstalk occurs at different levels of regulation, they converge in the regulation of PIN2 membrane trafficking in gravistimulated roots, supporting the notion that a temporally and spatially coordinated network of signal molecules could participate in the early phases of auxin polar transport during gravitropism.
Fil: Paris, Ramiro. Universidad Nacional de Mar del Plata; Argentina
Fil: Vazquez, María M.. Universidad Nacional de Mar del Plata; Argentina
Fil: Graziano, Magdalena. Universidad Nacional de Mar del Plata; Argentina
Fil: Terrile, Maria Cecilia. Universidad Nacional de Mar del Plata; Argentina
Fil: Miller, Nathan D.. University Of Wisconsin-madison; Estados Unidos
Fil: Spalding, Edgar P.. University Of Wisconsin-madison; Estados Unidos
Fil: Otegui, Marisa S.. University Of Wisconsin-madison;
Fil: Casalongue, Claudia. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
Materia
Arabidopsis
Auxin
Auxin transport
Gravitropism
Nitric oxide
Root
Tropic response
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88112
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/88112
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis rootsParis, RamiroVazquez, María M.Graziano, MagdalenaTerrile, Maria CeciliaMiller, Nathan D.Spalding, Edgar P.Otegui, Marisa S.Casalongue, ClaudiaArabidopsisAuxinAuxin transportGravitropismNitric oxideRootTropic responsehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1High-resolution and automated image analysis of individual roots demonstrated that endogenous nitric oxide (NO) contribute significantly to gravitropism of Arabidopsis roots. Lowering of endogenous NO concentrations strongly reduced and even reversed gravitropism, resulting in upward bending, without affecting root growth rate. Notably, the asymmetric accumulation of NOalong the upper and lower sides of roots correlated with a positive gravitropic response. Detection of NO by the specific DAF-FM DA fluorescent probe revealed that NO was higher at the lower side of horizontally-oriented roots returning to initial values 2h after the onset of gravistimulation. We demonstrate that NO promotes plasma membrane re-localization of PIN2 in epidermal cells, which is required during the early root gravitropic response. The dynamic and asymmetric localization of both auxin and NO is critical to regulate auxin polar transport during gravitropism. Our results collectively suggest that, although auxin and NO crosstalk occurs at different levels of regulation, they converge in the regulation of PIN2 membrane trafficking in gravistimulated roots, supporting the notion that a temporally and spatially coordinated network of signal molecules could participate in the early phases of auxin polar transport during gravitropism.Fil: Paris, Ramiro. Universidad Nacional de Mar del Plata; ArgentinaFil: Vazquez, María M.. Universidad Nacional de Mar del Plata; ArgentinaFil: Graziano, Magdalena. Universidad Nacional de Mar del Plata; ArgentinaFil: Terrile, Maria Cecilia. Universidad Nacional de Mar del Plata; ArgentinaFil: Miller, Nathan D.. University Of Wisconsin-madison; Estados UnidosFil: Spalding, Edgar P.. University Of Wisconsin-madison; Estados UnidosFil: Otegui, Marisa S.. University Of Wisconsin-madison;Fil: Casalongue, Claudia. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; ArgentinaFrontiers Media S.A.2018-04-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88112Paris, Ramiro; Vazquez, María M.; Graziano, Magdalena; Terrile, Maria Cecilia; Miller, Nathan D.; et al.; Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots; Frontiers Media S.A.; Frontiers in Plant Science; 9; 20-4-2018; 1-111664-462XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fpls.2018.00495/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2018.00495info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:26Zoai:ri.conicet.gov.ar:11336/88112instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:26.418CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots
title Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots
spellingShingle Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots
Paris, Ramiro
Arabidopsis
Auxin
Auxin transport
Gravitropism
Nitric oxide
Root
Tropic response
title_short Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots
title_full Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots
title_fullStr Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots
title_full_unstemmed Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots
title_sort Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots
dc.creator.none.fl_str_mv Paris, Ramiro
Vazquez, María M.
Graziano, Magdalena
Terrile, Maria Cecilia
Miller, Nathan D.
Spalding, Edgar P.
Otegui, Marisa S.
Casalongue, Claudia
author Paris, Ramiro
author_facet Paris, Ramiro
Vazquez, María M.
Graziano, Magdalena
Terrile, Maria Cecilia
Miller, Nathan D.
Spalding, Edgar P.
Otegui, Marisa S.
Casalongue, Claudia
author_role author
author2 Vazquez, María M.
Graziano, Magdalena
Terrile, Maria Cecilia
Miller, Nathan D.
Spalding, Edgar P.
Otegui, Marisa S.
Casalongue, Claudia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis
Auxin
Auxin transport
Gravitropism
Nitric oxide
Root
Tropic response
topic Arabidopsis
Auxin
Auxin transport
Gravitropism
Nitric oxide
Root
Tropic response
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv High-resolution and automated image analysis of individual roots demonstrated that endogenous nitric oxide (NO) contribute significantly to gravitropism of Arabidopsis roots. Lowering of endogenous NO concentrations strongly reduced and even reversed gravitropism, resulting in upward bending, without affecting root growth rate. Notably, the asymmetric accumulation of NOalong the upper and lower sides of roots correlated with a positive gravitropic response. Detection of NO by the specific DAF-FM DA fluorescent probe revealed that NO was higher at the lower side of horizontally-oriented roots returning to initial values 2h after the onset of gravistimulation. We demonstrate that NO promotes plasma membrane re-localization of PIN2 in epidermal cells, which is required during the early root gravitropic response. The dynamic and asymmetric localization of both auxin and NO is critical to regulate auxin polar transport during gravitropism. Our results collectively suggest that, although auxin and NO crosstalk occurs at different levels of regulation, they converge in the regulation of PIN2 membrane trafficking in gravistimulated roots, supporting the notion that a temporally and spatially coordinated network of signal molecules could participate in the early phases of auxin polar transport during gravitropism.
Fil: Paris, Ramiro. Universidad Nacional de Mar del Plata; Argentina
Fil: Vazquez, María M.. Universidad Nacional de Mar del Plata; Argentina
Fil: Graziano, Magdalena. Universidad Nacional de Mar del Plata; Argentina
Fil: Terrile, Maria Cecilia. Universidad Nacional de Mar del Plata; Argentina
Fil: Miller, Nathan D.. University Of Wisconsin-madison; Estados Unidos
Fil: Spalding, Edgar P.. University Of Wisconsin-madison; Estados Unidos
Fil: Otegui, Marisa S.. University Of Wisconsin-madison;
Fil: Casalongue, Claudia. Universidad Nacional de Mar del Plata; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
description High-resolution and automated image analysis of individual roots demonstrated that endogenous nitric oxide (NO) contribute significantly to gravitropism of Arabidopsis roots. Lowering of endogenous NO concentrations strongly reduced and even reversed gravitropism, resulting in upward bending, without affecting root growth rate. Notably, the asymmetric accumulation of NOalong the upper and lower sides of roots correlated with a positive gravitropic response. Detection of NO by the specific DAF-FM DA fluorescent probe revealed that NO was higher at the lower side of horizontally-oriented roots returning to initial values 2h after the onset of gravistimulation. We demonstrate that NO promotes plasma membrane re-localization of PIN2 in epidermal cells, which is required during the early root gravitropic response. The dynamic and asymmetric localization of both auxin and NO is critical to regulate auxin polar transport during gravitropism. Our results collectively suggest that, although auxin and NO crosstalk occurs at different levels of regulation, they converge in the regulation of PIN2 membrane trafficking in gravistimulated roots, supporting the notion that a temporally and spatially coordinated network of signal molecules could participate in the early phases of auxin polar transport during gravitropism.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88112
Paris, Ramiro; Vazquez, María M.; Graziano, Magdalena; Terrile, Maria Cecilia; Miller, Nathan D.; et al.; Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots; Frontiers Media S.A.; Frontiers in Plant Science; 9; 20-4-2018; 1-11
1664-462X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88112
identifier_str_mv Paris, Ramiro; Vazquez, María M.; Graziano, Magdalena; Terrile, Maria Cecilia; Miller, Nathan D.; et al.; Distribution of endogenous NO regulates early gravitropic response and PIN2 localization in arabidopsis roots; Frontiers Media S.A.; Frontiers in Plant Science; 9; 20-4-2018; 1-11
1664-462X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journal.frontiersin.org/article/10.3389/fpls.2018.00495/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fpls.2018.00495
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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