Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments

Autores
Figueroa, Ana Cristina; Díaz, María Soledad; Alasino, Roxana Valeria; Yang, Pablo; Soria, Néstor Walter; Beltramo, Dante Miguel
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Thecaphora frezii is a phytopathogenic fungus that infects Arachys hypogaea L. and produces peanut smut. It has three ontological stages teliospores, basidiospores, and hyphae. Microtubules are cellular structures that participate in various important cellular processes. In this work, we analyzed the presence and location of α-tubulin isotypes and enzymes that participate in tyrosination-detyrosination in the three stages of T. frezii. Although both tyrosinated and detyrosinated tubulin seem to be associated with a membrane fraction component that gives it a similar behavior to integral proteins, in the soluble cytosolic fraction, only detyrosinated tubulin was detected, not tyrosinated tubulin. The presence of α-tubulin was not detected using the monoclonal antibody DM1A as neither acetylated tubulin. The RNA-Seq analysis showed the presence of α, β, and γ-tubulins and the genes that codes for tyrosine-tubulin ligase and cytosolic carboxypeptidase 1, enzymes that are involved in post-translational modification processes. These sequences showed a high percentage of identity and homology with Ustilago maydis, Thecaphora thlaspeos, and Anthracocystis flocculosa. This is the first report for tubulins subpopulations and the cellular distribution in T. frezii, which together with the data obtained by RNA-Seq contribute to the knowledge of the pathogen, which will allow the development of control strategies
Fil: Figueroa, Ana Cristina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Díaz, María Soledad. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Yang, Pablo. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Soria, Néstor Walter. Universidad Católica de Córdoba; Argentina
Fil: Beltramo, Dante Miguel. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Materia
CYTOSQUELETON
DETYROSINATED TUBULIN
IMMUNOSTAINING
MRNA SEQUENCE
PEANUT SMUT
TYROSINATED TUBULIN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/222631
Acceder
id CONICETDig_ffb7c8c9da6eb2d092a970de755e319d
oai_identifier_str oai:ri.conicet.gov.ar:11336/222631
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developmentsFigueroa, Ana CristinaDíaz, María SoledadAlasino, Roxana ValeriaYang, PabloSoria, Néstor WalterBeltramo, Dante MiguelCYTOSQUELETONDETYROSINATED TUBULINIMMUNOSTAININGMRNA SEQUENCEPEANUT SMUTTYROSINATED TUBULINhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Thecaphora frezii is a phytopathogenic fungus that infects Arachys hypogaea L. and produces peanut smut. It has three ontological stages teliospores, basidiospores, and hyphae. Microtubules are cellular structures that participate in various important cellular processes. In this work, we analyzed the presence and location of α-tubulin isotypes and enzymes that participate in tyrosination-detyrosination in the three stages of T. frezii. Although both tyrosinated and detyrosinated tubulin seem to be associated with a membrane fraction component that gives it a similar behavior to integral proteins, in the soluble cytosolic fraction, only detyrosinated tubulin was detected, not tyrosinated tubulin. The presence of α-tubulin was not detected using the monoclonal antibody DM1A as neither acetylated tubulin. The RNA-Seq analysis showed the presence of α, β, and γ-tubulins and the genes that codes for tyrosine-tubulin ligase and cytosolic carboxypeptidase 1, enzymes that are involved in post-translational modification processes. These sequences showed a high percentage of identity and homology with Ustilago maydis, Thecaphora thlaspeos, and Anthracocystis flocculosa. This is the first report for tubulins subpopulations and the cellular distribution in T. frezii, which together with the data obtained by RNA-Seq contribute to the knowledge of the pathogen, which will allow the development of control strategiesFil: Figueroa, Ana Cristina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Díaz, María Soledad. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaFil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Yang, Pablo. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Soria, Néstor Walter. Universidad Católica de Córdoba; ArgentinaFil: Beltramo, Dante Miguel. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; ArgentinaWiley Blackwell Publishing, Inc2022-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/222631Figueroa, Ana Cristina; Díaz, María Soledad; Alasino, Roxana Valeria; Yang, Pablo; Soria, Néstor Walter; et al.; Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 98; 11; 10-20220168-6496CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiac121info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/article/98/11/fiac121/6753202info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:03:20Zoai:ri.conicet.gov.ar:11336/222631instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:03:20.614CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
spellingShingle Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
Figueroa, Ana Cristina
CYTOSQUELETON
DETYROSINATED TUBULIN
IMMUNOSTAINING
MRNA SEQUENCE
PEANUT SMUT
TYROSINATED TUBULIN
title_short Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_full Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_fullStr Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_full_unstemmed Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
title_sort Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments
dc.creator.none.fl_str_mv Figueroa, Ana Cristina
Díaz, María Soledad
Alasino, Roxana Valeria
Yang, Pablo
Soria, Néstor Walter
Beltramo, Dante Miguel
author Figueroa, Ana Cristina
author_facet Figueroa, Ana Cristina
Díaz, María Soledad
Alasino, Roxana Valeria
Yang, Pablo
Soria, Néstor Walter
Beltramo, Dante Miguel
author_role author
author2 Díaz, María Soledad
Alasino, Roxana Valeria
Yang, Pablo
Soria, Néstor Walter
Beltramo, Dante Miguel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CYTOSQUELETON
DETYROSINATED TUBULIN
IMMUNOSTAINING
MRNA SEQUENCE
PEANUT SMUT
TYROSINATED TUBULIN
topic CYTOSQUELETON
DETYROSINATED TUBULIN
IMMUNOSTAINING
MRNA SEQUENCE
PEANUT SMUT
TYROSINATED TUBULIN
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Thecaphora frezii is a phytopathogenic fungus that infects Arachys hypogaea L. and produces peanut smut. It has three ontological stages teliospores, basidiospores, and hyphae. Microtubules are cellular structures that participate in various important cellular processes. In this work, we analyzed the presence and location of α-tubulin isotypes and enzymes that participate in tyrosination-detyrosination in the three stages of T. frezii. Although both tyrosinated and detyrosinated tubulin seem to be associated with a membrane fraction component that gives it a similar behavior to integral proteins, in the soluble cytosolic fraction, only detyrosinated tubulin was detected, not tyrosinated tubulin. The presence of α-tubulin was not detected using the monoclonal antibody DM1A as neither acetylated tubulin. The RNA-Seq analysis showed the presence of α, β, and γ-tubulins and the genes that codes for tyrosine-tubulin ligase and cytosolic carboxypeptidase 1, enzymes that are involved in post-translational modification processes. These sequences showed a high percentage of identity and homology with Ustilago maydis, Thecaphora thlaspeos, and Anthracocystis flocculosa. This is the first report for tubulins subpopulations and the cellular distribution in T. frezii, which together with the data obtained by RNA-Seq contribute to the knowledge of the pathogen, which will allow the development of control strategies
Fil: Figueroa, Ana Cristina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Díaz, María Soledad. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
Fil: Alasino, Roxana Valeria. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Yang, Pablo. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Soria, Néstor Walter. Universidad Católica de Córdoba; Argentina
Fil: Beltramo, Dante Miguel. Universidad Católica de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Provincia de Córdoba. Ministerio de Ciencia y Técnica. Centro de Excelencia en Productos y Procesos de Córdoba; Argentina
description Thecaphora frezii is a phytopathogenic fungus that infects Arachys hypogaea L. and produces peanut smut. It has three ontological stages teliospores, basidiospores, and hyphae. Microtubules are cellular structures that participate in various important cellular processes. In this work, we analyzed the presence and location of α-tubulin isotypes and enzymes that participate in tyrosination-detyrosination in the three stages of T. frezii. Although both tyrosinated and detyrosinated tubulin seem to be associated with a membrane fraction component that gives it a similar behavior to integral proteins, in the soluble cytosolic fraction, only detyrosinated tubulin was detected, not tyrosinated tubulin. The presence of α-tubulin was not detected using the monoclonal antibody DM1A as neither acetylated tubulin. The RNA-Seq analysis showed the presence of α, β, and γ-tubulins and the genes that codes for tyrosine-tubulin ligase and cytosolic carboxypeptidase 1, enzymes that are involved in post-translational modification processes. These sequences showed a high percentage of identity and homology with Ustilago maydis, Thecaphora thlaspeos, and Anthracocystis flocculosa. This is the first report for tubulins subpopulations and the cellular distribution in T. frezii, which together with the data obtained by RNA-Seq contribute to the knowledge of the pathogen, which will allow the development of control strategies
publishDate 2022
dc.date.none.fl_str_mv 2022-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/222631
Figueroa, Ana Cristina; Díaz, María Soledad; Alasino, Roxana Valeria; Yang, Pablo; Soria, Néstor Walter; et al.; Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 98; 11; 10-2022
0168-6496
CONICET Digital
CONICET
url http://hdl.handle.net/11336/222631
identifier_str_mv Figueroa, Ana Cristina; Díaz, María Soledad; Alasino, Roxana Valeria; Yang, Pablo; Soria, Néstor Walter; et al.; Expression and localization of tubulin isotypes and its mRNAs during Thecaphora frezii developments; Wiley Blackwell Publishing, Inc; Fems Microbiology Ecology; 98; 11; 10-2022
0168-6496
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiac121
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsec/article/98/11/fiac121/6753202
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781263746170880
score 12.982451

Publicaciones similares