Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds

Autores
Schabes, Fanny Isabel; Sigstad, Emma Elizabeth
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To establish the best conditions for germination, calorimetric experiments were conducted with individual soybean seeds (Glycine max [L.] Merr.) under different conditions of imbibition at 24.7 °C. The calorimetric curves were analysed for imbibition and metabolic processes and compared with imbibition curves to establish a general methodology that could be used to evaluate inter- and intra-species physical and physiological variability. Measurements of pH were performed during the determination of the imbibition curves. The best experimental method for calorimetric investigations of seed germination is to insert the seed in 1% agar instead of placing the seed over wetted filter paper disks. Correlation of imbibition experiments and pH results with mass specific enthalpy of imbibition (determined in a KCN solution) or mass specific enthalpy of germination when seeds were germinated in 1% agar allowed determination of the water content needed for soybean seeds of the cultivar studied (A7636 RG) to activate their metabolic machinery (74-80% or 2.5-3 h) and the moment in which they are ready for root protrusion (122% or 9 h). The method presented here should be useful for evaluating soybean coat permeability to water and other factors related to seed damage before and during harvest and storage. © 2006 Elsevier B.V. All rights reserved.
Fil: Schabes, Fanny Isabel. Universidad Nacional de Tucumán; Argentina
Fil: Sigstad, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán; Argentina
Materia
GLYCINE MAX
ISOTHERMAL CALORIMETRY
SEED GERMINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/100867

id CONICETDig_fe5c08985d7b87e57a999a3a5c740d78
oai_identifier_str oai:ri.conicet.gov.ar:11336/100867
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seedsSchabes, Fanny IsabelSigstad, Emma ElizabethGLYCINE MAXISOTHERMAL CALORIMETRYSEED GERMINATIONhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4To establish the best conditions for germination, calorimetric experiments were conducted with individual soybean seeds (Glycine max [L.] Merr.) under different conditions of imbibition at 24.7 °C. The calorimetric curves were analysed for imbibition and metabolic processes and compared with imbibition curves to establish a general methodology that could be used to evaluate inter- and intra-species physical and physiological variability. Measurements of pH were performed during the determination of the imbibition curves. The best experimental method for calorimetric investigations of seed germination is to insert the seed in 1% agar instead of placing the seed over wetted filter paper disks. Correlation of imbibition experiments and pH results with mass specific enthalpy of imbibition (determined in a KCN solution) or mass specific enthalpy of germination when seeds were germinated in 1% agar allowed determination of the water content needed for soybean seeds of the cultivar studied (A7636 RG) to activate their metabolic machinery (74-80% or 2.5-3 h) and the moment in which they are ready for root protrusion (122% or 9 h). The method presented here should be useful for evaluating soybean coat permeability to water and other factors related to seed damage before and during harvest and storage. © 2006 Elsevier B.V. All rights reserved.Fil: Schabes, Fanny Isabel. Universidad Nacional de Tucumán; ArgentinaFil: Sigstad, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán; ArgentinaElsevier Science2006-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100867Schabes, Fanny Isabel; Sigstad, Emma Elizabeth; Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds; Elsevier Science; Thermochimica Acta; 450; 1-2; 11-2006; 96-1010040-6031CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.tca.2006.07.026info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0040603106004503?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:00Zoai:ri.conicet.gov.ar:11336/100867instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:01.156CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
title Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
spellingShingle Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
Schabes, Fanny Isabel
GLYCINE MAX
ISOTHERMAL CALORIMETRY
SEED GERMINATION
title_short Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
title_full Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
title_fullStr Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
title_full_unstemmed Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
title_sort Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
dc.creator.none.fl_str_mv Schabes, Fanny Isabel
Sigstad, Emma Elizabeth
author Schabes, Fanny Isabel
author_facet Schabes, Fanny Isabel
Sigstad, Emma Elizabeth
author_role author
author2 Sigstad, Emma Elizabeth
author2_role author
dc.subject.none.fl_str_mv GLYCINE MAX
ISOTHERMAL CALORIMETRY
SEED GERMINATION
topic GLYCINE MAX
ISOTHERMAL CALORIMETRY
SEED GERMINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv To establish the best conditions for germination, calorimetric experiments were conducted with individual soybean seeds (Glycine max [L.] Merr.) under different conditions of imbibition at 24.7 °C. The calorimetric curves were analysed for imbibition and metabolic processes and compared with imbibition curves to establish a general methodology that could be used to evaluate inter- and intra-species physical and physiological variability. Measurements of pH were performed during the determination of the imbibition curves. The best experimental method for calorimetric investigations of seed germination is to insert the seed in 1% agar instead of placing the seed over wetted filter paper disks. Correlation of imbibition experiments and pH results with mass specific enthalpy of imbibition (determined in a KCN solution) or mass specific enthalpy of germination when seeds were germinated in 1% agar allowed determination of the water content needed for soybean seeds of the cultivar studied (A7636 RG) to activate their metabolic machinery (74-80% or 2.5-3 h) and the moment in which they are ready for root protrusion (122% or 9 h). The method presented here should be useful for evaluating soybean coat permeability to water and other factors related to seed damage before and during harvest and storage. © 2006 Elsevier B.V. All rights reserved.
Fil: Schabes, Fanny Isabel. Universidad Nacional de Tucumán; Argentina
Fil: Sigstad, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán; Argentina
description To establish the best conditions for germination, calorimetric experiments were conducted with individual soybean seeds (Glycine max [L.] Merr.) under different conditions of imbibition at 24.7 °C. The calorimetric curves were analysed for imbibition and metabolic processes and compared with imbibition curves to establish a general methodology that could be used to evaluate inter- and intra-species physical and physiological variability. Measurements of pH were performed during the determination of the imbibition curves. The best experimental method for calorimetric investigations of seed germination is to insert the seed in 1% agar instead of placing the seed over wetted filter paper disks. Correlation of imbibition experiments and pH results with mass specific enthalpy of imbibition (determined in a KCN solution) or mass specific enthalpy of germination when seeds were germinated in 1% agar allowed determination of the water content needed for soybean seeds of the cultivar studied (A7636 RG) to activate their metabolic machinery (74-80% or 2.5-3 h) and the moment in which they are ready for root protrusion (122% or 9 h). The method presented here should be useful for evaluating soybean coat permeability to water and other factors related to seed damage before and during harvest and storage. © 2006 Elsevier B.V. All rights reserved.
publishDate 2006
dc.date.none.fl_str_mv 2006-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/100867
Schabes, Fanny Isabel; Sigstad, Emma Elizabeth; Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds; Elsevier Science; Thermochimica Acta; 450; 1-2; 11-2006; 96-101
0040-6031
CONICET Digital
CONICET
url http://hdl.handle.net/11336/100867
identifier_str_mv Schabes, Fanny Isabel; Sigstad, Emma Elizabeth; Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds; Elsevier Science; Thermochimica Acta; 450; 1-2; 11-2006; 96-101
0040-6031
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tca.2006.07.026
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0040603106004503?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613798323814400
score 13.070432