Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds
- Autores
- Schabes, Fanny Isabel; Sigstad, Emma Elizabeth
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- To establish the best conditions for germination, calorimetric experiments were conducted with individual soybean seeds (Glycine max [L.] Merr.) under different conditions of imbibition at 24.7 °C. The calorimetric curves were analysed for imbibition and metabolic processes and compared with imbibition curves to establish a general methodology that could be used to evaluate inter- and intra-species physical and physiological variability. Measurements of pH were performed during the determination of the imbibition curves. The best experimental method for calorimetric investigations of seed germination is to insert the seed in 1% agar instead of placing the seed over wetted filter paper disks. Correlation of imbibition experiments and pH results with mass specific enthalpy of imbibition (determined in a KCN solution) or mass specific enthalpy of germination when seeds were germinated in 1% agar allowed determination of the water content needed for soybean seeds of the cultivar studied (A7636 RG) to activate their metabolic machinery (74-80% or 2.5-3 h) and the moment in which they are ready for root protrusion (122% or 9 h). The method presented here should be useful for evaluating soybean coat permeability to water and other factors related to seed damage before and during harvest and storage. © 2006 Elsevier B.V. All rights reserved.
Fil: Schabes, Fanny Isabel. Universidad Nacional de Tucumán; Argentina
Fil: Sigstad, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán; Argentina - Materia
-
GLYCINE MAX
ISOTHERMAL CALORIMETRY
SEED GERMINATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/100867
Ver los metadatos del registro completo
id |
CONICETDig_fe5c08985d7b87e57a999a3a5c740d78 |
---|---|
oai_identifier_str |
oai:ri.conicet.gov.ar:11336/100867 |
network_acronym_str |
CONICETDig |
repository_id_str |
3498 |
network_name_str |
CONICET Digital (CONICET) |
spelling |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seedsSchabes, Fanny IsabelSigstad, Emma ElizabethGLYCINE MAXISOTHERMAL CALORIMETRYSEED GERMINATIONhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4To establish the best conditions for germination, calorimetric experiments were conducted with individual soybean seeds (Glycine max [L.] Merr.) under different conditions of imbibition at 24.7 °C. The calorimetric curves were analysed for imbibition and metabolic processes and compared with imbibition curves to establish a general methodology that could be used to evaluate inter- and intra-species physical and physiological variability. Measurements of pH were performed during the determination of the imbibition curves. The best experimental method for calorimetric investigations of seed germination is to insert the seed in 1% agar instead of placing the seed over wetted filter paper disks. Correlation of imbibition experiments and pH results with mass specific enthalpy of imbibition (determined in a KCN solution) or mass specific enthalpy of germination when seeds were germinated in 1% agar allowed determination of the water content needed for soybean seeds of the cultivar studied (A7636 RG) to activate their metabolic machinery (74-80% or 2.5-3 h) and the moment in which they are ready for root protrusion (122% or 9 h). The method presented here should be useful for evaluating soybean coat permeability to water and other factors related to seed damage before and during harvest and storage. © 2006 Elsevier B.V. All rights reserved.Fil: Schabes, Fanny Isabel. Universidad Nacional de Tucumán; ArgentinaFil: Sigstad, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán; ArgentinaElsevier Science2006-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/100867Schabes, Fanny Isabel; Sigstad, Emma Elizabeth; Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds; Elsevier Science; Thermochimica Acta; 450; 1-2; 11-2006; 96-1010040-6031CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.tca.2006.07.026info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0040603106004503?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:01:00Zoai:ri.conicet.gov.ar:11336/100867instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:01:01.156CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds |
title |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds |
spellingShingle |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds Schabes, Fanny Isabel GLYCINE MAX ISOTHERMAL CALORIMETRY SEED GERMINATION |
title_short |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds |
title_full |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds |
title_fullStr |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds |
title_full_unstemmed |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds |
title_sort |
Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds |
dc.creator.none.fl_str_mv |
Schabes, Fanny Isabel Sigstad, Emma Elizabeth |
author |
Schabes, Fanny Isabel |
author_facet |
Schabes, Fanny Isabel Sigstad, Emma Elizabeth |
author_role |
author |
author2 |
Sigstad, Emma Elizabeth |
author2_role |
author |
dc.subject.none.fl_str_mv |
GLYCINE MAX ISOTHERMAL CALORIMETRY SEED GERMINATION |
topic |
GLYCINE MAX ISOTHERMAL CALORIMETRY SEED GERMINATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
To establish the best conditions for germination, calorimetric experiments were conducted with individual soybean seeds (Glycine max [L.] Merr.) under different conditions of imbibition at 24.7 °C. The calorimetric curves were analysed for imbibition and metabolic processes and compared with imbibition curves to establish a general methodology that could be used to evaluate inter- and intra-species physical and physiological variability. Measurements of pH were performed during the determination of the imbibition curves. The best experimental method for calorimetric investigations of seed germination is to insert the seed in 1% agar instead of placing the seed over wetted filter paper disks. Correlation of imbibition experiments and pH results with mass specific enthalpy of imbibition (determined in a KCN solution) or mass specific enthalpy of germination when seeds were germinated in 1% agar allowed determination of the water content needed for soybean seeds of the cultivar studied (A7636 RG) to activate their metabolic machinery (74-80% or 2.5-3 h) and the moment in which they are ready for root protrusion (122% or 9 h). The method presented here should be useful for evaluating soybean coat permeability to water and other factors related to seed damage before and during harvest and storage. © 2006 Elsevier B.V. All rights reserved. Fil: Schabes, Fanny Isabel. Universidad Nacional de Tucumán; Argentina Fil: Sigstad, Emma Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán; Argentina |
description |
To establish the best conditions for germination, calorimetric experiments were conducted with individual soybean seeds (Glycine max [L.] Merr.) under different conditions of imbibition at 24.7 °C. The calorimetric curves were analysed for imbibition and metabolic processes and compared with imbibition curves to establish a general methodology that could be used to evaluate inter- and intra-species physical and physiological variability. Measurements of pH were performed during the determination of the imbibition curves. The best experimental method for calorimetric investigations of seed germination is to insert the seed in 1% agar instead of placing the seed over wetted filter paper disks. Correlation of imbibition experiments and pH results with mass specific enthalpy of imbibition (determined in a KCN solution) or mass specific enthalpy of germination when seeds were germinated in 1% agar allowed determination of the water content needed for soybean seeds of the cultivar studied (A7636 RG) to activate their metabolic machinery (74-80% or 2.5-3 h) and the moment in which they are ready for root protrusion (122% or 9 h). The method presented here should be useful for evaluating soybean coat permeability to water and other factors related to seed damage before and during harvest and storage. © 2006 Elsevier B.V. All rights reserved. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-11 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/100867 Schabes, Fanny Isabel; Sigstad, Emma Elizabeth; Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds; Elsevier Science; Thermochimica Acta; 450; 1-2; 11-2006; 96-101 0040-6031 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/100867 |
identifier_str_mv |
Schabes, Fanny Isabel; Sigstad, Emma Elizabeth; Optimizing conditions to study seed germination by calorimetry using soybean (Glycine max [L.] Merr.) seeds; Elsevier Science; Thermochimica Acta; 450; 1-2; 11-2006; 96-101 0040-6031 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.tca.2006.07.026 info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0040603106004503?via%3Dihub |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science |
publisher.none.fl_str_mv |
Elsevier Science |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
_version_ |
1844613798323814400 |
score |
13.070432 |