On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization

Autores
Henestrosa, Cecilia Mariel; Peralta, Cecilia Mariana; Aragón, Leslie Mary; Fernandez, Liliana Patricia; Acosta, Maria Gimena
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work presents a simple and sensitive flow injection fluorimetric methodfor the determination of carbamazepine (CBZ) in pharmaceutical samples. Itis based on the fluorescence signal measurement of the product generated byphotoinduced (λex = 251 nm; λem = 434 nm) using a flow injection manifoldcontaining an on-line homemade photoreactor. In order to optimize the experimental setup, the variable conditions were studied using a multivariateoptimization method, thus finding the set of optimum parameters accordingto the evaluated responses (sensitivity and sampling frequency) and minimizing the number of experiments performed. Under optimal experimentalconditions, linear relationship with good correlation coefficient (0.9956) wasfound between the fluorescence intensity and CBZ concentration in the rangeof 0.13 - 40 µg∙mL−1. The limit of detection was 0.04 µg∙mL−1. The precision ofthe method was satisfactory; the values of relative standard deviations werebetter than 4.36%. The proposed method was validated and successfully applied to the determination of CBZ in pharmaceuticals with good accuracy;being the recovery values from 89.45% - 112.20%.
Fil: Henestrosa, Cecilia Mariel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Aragón, Leslie Mary. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
ON LINE PHOTOINDUCED FLUORESCECNE
CARBAMAZEPINE
MULTIVARIATE OPTIMIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104910
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/104910
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate OptimizationHenestrosa, Cecilia MarielPeralta, Cecilia MarianaAragón, Leslie MaryFernandez, Liliana PatriciaAcosta, Maria GimenaON LINE PHOTOINDUCED FLUORESCECNECARBAMAZEPINEMULTIVARIATE OPTIMIZATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1This work presents a simple and sensitive flow injection fluorimetric methodfor the determination of carbamazepine (CBZ) in pharmaceutical samples. Itis based on the fluorescence signal measurement of the product generated byphotoinduced (λex = 251 nm; λem = 434 nm) using a flow injection manifoldcontaining an on-line homemade photoreactor. In order to optimize the experimental setup, the variable conditions were studied using a multivariateoptimization method, thus finding the set of optimum parameters accordingto the evaluated responses (sensitivity and sampling frequency) and minimizing the number of experiments performed. Under optimal experimentalconditions, linear relationship with good correlation coefficient (0.9956) wasfound between the fluorescence intensity and CBZ concentration in the rangeof 0.13 - 40 µg∙mL−1. The limit of detection was 0.04 µg∙mL−1. The precision ofthe method was satisfactory; the values of relative standard deviations werebetter than 4.36%. The proposed method was validated and successfully applied to the determination of CBZ in pharmaceuticals with good accuracy;being the recovery values from 89.45% - 112.20%.Fil: Henestrosa, Cecilia Mariel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; ArgentinaFil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Aragón, Leslie Mary. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaScientific Research2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104910Henestrosa, Cecilia Mariel; Peralta, Cecilia Mariana; Aragón, Leslie Mary; Fernandez, Liliana Patricia; Acosta, Maria Gimena; On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization; Scientific Research; American Journal of Analytical Chemistry; 10; 11; 11-2019; 513-5272156-8278CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/doi.aspx?DOI=10.4236/ajac.2019.1011037info:eu-repo/semantics/altIdentifier/doi/10.4236/ajac.2019.1011037info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:06:27Zoai:ri.conicet.gov.ar:11336/104910instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:06:27.574CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
title On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
spellingShingle On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
Henestrosa, Cecilia Mariel
ON LINE PHOTOINDUCED FLUORESCECNE
CARBAMAZEPINE
MULTIVARIATE OPTIMIZATION
title_short On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
title_full On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
title_fullStr On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
title_full_unstemmed On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
title_sort On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization
dc.creator.none.fl_str_mv Henestrosa, Cecilia Mariel
Peralta, Cecilia Mariana
Aragón, Leslie Mary
Fernandez, Liliana Patricia
Acosta, Maria Gimena
author Henestrosa, Cecilia Mariel
author_facet Henestrosa, Cecilia Mariel
Peralta, Cecilia Mariana
Aragón, Leslie Mary
Fernandez, Liliana Patricia
Acosta, Maria Gimena
author_role author
author2 Peralta, Cecilia Mariana
Aragón, Leslie Mary
Fernandez, Liliana Patricia
Acosta, Maria Gimena
author2_role author
author
author
author
dc.subject.none.fl_str_mv ON LINE PHOTOINDUCED FLUORESCECNE
CARBAMAZEPINE
MULTIVARIATE OPTIMIZATION
topic ON LINE PHOTOINDUCED FLUORESCECNE
CARBAMAZEPINE
MULTIVARIATE OPTIMIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv This work presents a simple and sensitive flow injection fluorimetric methodfor the determination of carbamazepine (CBZ) in pharmaceutical samples. Itis based on the fluorescence signal measurement of the product generated byphotoinduced (λex = 251 nm; λem = 434 nm) using a flow injection manifoldcontaining an on-line homemade photoreactor. In order to optimize the experimental setup, the variable conditions were studied using a multivariateoptimization method, thus finding the set of optimum parameters accordingto the evaluated responses (sensitivity and sampling frequency) and minimizing the number of experiments performed. Under optimal experimentalconditions, linear relationship with good correlation coefficient (0.9956) wasfound between the fluorescence intensity and CBZ concentration in the rangeof 0.13 - 40 µg∙mL−1. The limit of detection was 0.04 µg∙mL−1. The precision ofthe method was satisfactory; the values of relative standard deviations werebetter than 4.36%. The proposed method was validated and successfully applied to the determination of CBZ in pharmaceuticals with good accuracy;being the recovery values from 89.45% - 112.20%.
Fil: Henestrosa, Cecilia Mariel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Aragón, Leslie Mary. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description This work presents a simple and sensitive flow injection fluorimetric methodfor the determination of carbamazepine (CBZ) in pharmaceutical samples. Itis based on the fluorescence signal measurement of the product generated byphotoinduced (λex = 251 nm; λem = 434 nm) using a flow injection manifoldcontaining an on-line homemade photoreactor. In order to optimize the experimental setup, the variable conditions were studied using a multivariateoptimization method, thus finding the set of optimum parameters accordingto the evaluated responses (sensitivity and sampling frequency) and minimizing the number of experiments performed. Under optimal experimentalconditions, linear relationship with good correlation coefficient (0.9956) wasfound between the fluorescence intensity and CBZ concentration in the rangeof 0.13 - 40 µg∙mL−1. The limit of detection was 0.04 µg∙mL−1. The precision ofthe method was satisfactory; the values of relative standard deviations werebetter than 4.36%. The proposed method was validated and successfully applied to the determination of CBZ in pharmaceuticals with good accuracy;being the recovery values from 89.45% - 112.20%.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104910
Henestrosa, Cecilia Mariel; Peralta, Cecilia Mariana; Aragón, Leslie Mary; Fernandez, Liliana Patricia; Acosta, Maria Gimena; On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization; Scientific Research; American Journal of Analytical Chemistry; 10; 11; 11-2019; 513-527
2156-8278
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104910
identifier_str_mv Henestrosa, Cecilia Mariel; Peralta, Cecilia Mariana; Aragón, Leslie Mary; Fernandez, Liliana Patricia; Acosta, Maria Gimena; On-Line Photoinduced Fluorescence for Carbamazepine Determination after Multivariate Optimization; Scientific Research; American Journal of Analytical Chemistry; 10; 11; 11-2019; 513-527
2156-8278
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/doi.aspx?DOI=10.4236/ajac.2019.1011037
info:eu-repo/semantics/altIdentifier/doi/10.4236/ajac.2019.1011037
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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