Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites

Autores
Bourguignon, Natalia; Fuentes, María Soledad; Benimeli, Claudia Susana; Cuozzo, Sergio Antonio; Amoroso, Maria Julia del R.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Streptomyces strains isolated from pesticide-contaminated sediments of Santiago del Estero, Argentina were able to grow in the presence of 1.66 mg L−1 of methoxychlor (MTX). Streptomyces sp. A14 showed the best growth in the presence of MTX in culture medium at 30 °C and pH 7. When soil microcosms were contaminated with MTX and inoculated with Streptomyces sp. A14, a decrease in MTX was detected in both concentrations assayed (8.33and 16.60 mg kg−1). This actinobacterium was able to remove the pesticide, reaching its maximum removal percentages (40% and 76%) after 28 days of incubation. Methoxychlor was gradually converted into 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethane, 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethylene, 1-chloro-2,2-bis(4-methoxyphenyl)ethane, and 2,2-bis(4-hidroxyphenyl)acetonitrile, indicating that MTX is dominantly degraded by dechlorination, dehydrogenation and CN-replacement, resulting in the production of several major degradation products. This is an interesting proposal as an alternative method for soil cleanup. This is the first report of methoxychlor degradation using a native Streptomyces strain under aerobic conditions.
Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina
Materia
Bioremediation
Streptomyces
Methoxychlor
Metabolites
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/12966
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/12966
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolitesBourguignon, NataliaFuentes, María SoledadBenimeli, Claudia SusanaCuozzo, Sergio AntonioAmoroso, Maria Julia del R.BioremediationStreptomycesMethoxychlorMetaboliteshttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Streptomyces strains isolated from pesticide-contaminated sediments of Santiago del Estero, Argentina were able to grow in the presence of 1.66 mg L−1 of methoxychlor (MTX). Streptomyces sp. A14 showed the best growth in the presence of MTX in culture medium at 30 °C and pH 7. When soil microcosms were contaminated with MTX and inoculated with Streptomyces sp. A14, a decrease in MTX was detected in both concentrations assayed (8.33and 16.60 mg kg−1). This actinobacterium was able to remove the pesticide, reaching its maximum removal percentages (40% and 76%) after 28 days of incubation. Methoxychlor was gradually converted into 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethane, 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethylene, 1-chloro-2,2-bis(4-methoxyphenyl)ethane, and 2,2-bis(4-hidroxyphenyl)acetonitrile, indicating that MTX is dominantly degraded by dechlorination, dehydrogenation and CN-replacement, resulting in the production of several major degradation products. This is an interesting proposal as an alternative method for soil cleanup. This is the first report of methoxychlor degradation using a native Streptomyces strain under aerobic conditions.Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); ArgentinaFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina. Universidad del Norte Santo Tomás de Aquino; ArgentinaFil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad del Norte Santo Tomás de Aquino; ArgentinaElsevier2014-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/12966Bourguignon, Natalia; Fuentes, María Soledad; Benimeli, Claudia Susana; Cuozzo, Sergio Antonio; Amoroso, Maria Julia del R.; Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites; Elsevier; International Biodeterioration And Biodegradation; 96; 12-2014; 80–860964-8305enginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2014.09.016info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830514002868info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:09:27Zoai:ri.conicet.gov.ar:11336/12966instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:09:27.311CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites
title Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites
spellingShingle Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites
Bourguignon, Natalia
Bioremediation
Streptomyces
Methoxychlor
Metabolites
title_short Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites
title_full Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites
title_fullStr Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites
title_full_unstemmed Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites
title_sort Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites
dc.creator.none.fl_str_mv Bourguignon, Natalia
Fuentes, María Soledad
Benimeli, Claudia Susana
Cuozzo, Sergio Antonio
Amoroso, Maria Julia del R.
author Bourguignon, Natalia
author_facet Bourguignon, Natalia
Fuentes, María Soledad
Benimeli, Claudia Susana
Cuozzo, Sergio Antonio
Amoroso, Maria Julia del R.
author_role author
author2 Fuentes, María Soledad
Benimeli, Claudia Susana
Cuozzo, Sergio Antonio
Amoroso, Maria Julia del R.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bioremediation
Streptomyces
Methoxychlor
Metabolites
topic Bioremediation
Streptomyces
Methoxychlor
Metabolites
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Streptomyces strains isolated from pesticide-contaminated sediments of Santiago del Estero, Argentina were able to grow in the presence of 1.66 mg L−1 of methoxychlor (MTX). Streptomyces sp. A14 showed the best growth in the presence of MTX in culture medium at 30 °C and pH 7. When soil microcosms were contaminated with MTX and inoculated with Streptomyces sp. A14, a decrease in MTX was detected in both concentrations assayed (8.33and 16.60 mg kg−1). This actinobacterium was able to remove the pesticide, reaching its maximum removal percentages (40% and 76%) after 28 days of incubation. Methoxychlor was gradually converted into 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethane, 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethylene, 1-chloro-2,2-bis(4-methoxyphenyl)ethane, and 2,2-bis(4-hidroxyphenyl)acetonitrile, indicating that MTX is dominantly degraded by dechlorination, dehydrogenation and CN-replacement, resulting in the production of several major degradation products. This is an interesting proposal as an alternative method for soil cleanup. This is the first report of methoxychlor degradation using a native Streptomyces strain under aerobic conditions.
Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Planta Piloto de Procesos Industriales Microbiológicos (i); Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina. Universidad del Norte Santo Tomás de Aquino; Argentina
description Streptomyces strains isolated from pesticide-contaminated sediments of Santiago del Estero, Argentina were able to grow in the presence of 1.66 mg L−1 of methoxychlor (MTX). Streptomyces sp. A14 showed the best growth in the presence of MTX in culture medium at 30 °C and pH 7. When soil microcosms were contaminated with MTX and inoculated with Streptomyces sp. A14, a decrease in MTX was detected in both concentrations assayed (8.33and 16.60 mg kg−1). This actinobacterium was able to remove the pesticide, reaching its maximum removal percentages (40% and 76%) after 28 days of incubation. Methoxychlor was gradually converted into 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethane, 1,1-dichloro-2,2-bis(4-methoxyphenyl)ethylene, 1-chloro-2,2-bis(4-methoxyphenyl)ethane, and 2,2-bis(4-hidroxyphenyl)acetonitrile, indicating that MTX is dominantly degraded by dechlorination, dehydrogenation and CN-replacement, resulting in the production of several major degradation products. This is an interesting proposal as an alternative method for soil cleanup. This is the first report of methoxychlor degradation using a native Streptomyces strain under aerobic conditions.
publishDate 2014
dc.date.none.fl_str_mv 2014-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/12966
Bourguignon, Natalia; Fuentes, María Soledad; Benimeli, Claudia Susana; Cuozzo, Sergio Antonio; Amoroso, Maria Julia del R.; Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites; Elsevier; International Biodeterioration And Biodegradation; 96; 12-2014; 80–86
0964-8305
url http://hdl.handle.net/11336/12966
identifier_str_mv Bourguignon, Natalia; Fuentes, María Soledad; Benimeli, Claudia Susana; Cuozzo, Sergio Antonio; Amoroso, Maria Julia del R.; Aerobic removal of methoxychlor by a native Streptomyces strain: Identification of intermediate metabolites; Elsevier; International Biodeterioration And Biodegradation; 96; 12-2014; 80–86
0964-8305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ibiod.2014.09.016
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0964830514002868
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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