Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14

Autores
Bourguignon, Natalia; Cuozzo, Sergio Antonio; Fuentes, María Soledad; Benimeli, Claudia Susana; Amoroso, Maria Julia del R.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Organochlorine pesticides (OP) have aroused global concern due to their long persistence, low biodegradability, wide range distribution in the environment and chronic adverse effect on wildlife and humans. Methoxychlor (MTX) is a toxic OP that was used in industrial and agricultural activities and for the malaria control. Although the use of MTX has been internationally banned it can still be detected in the environment like in the northwest of Argentina. Bioremediation offers the possibility to environment cleanup of pollutants, such as OP, using natural biological activity. However, there is little information available about microbial MTX degradation. Actinobacteria have a great potential for bioremediation of toxic compounds, in addition strains of Streptomyces genus may be well suited for soil inoculation as a consequence of their mycelial growth habit, relatively rapid rates of growth and colonization of semi-selective substrates. In our laboratory, we isolated an actinobacteria strain from OP-contaminated soil in Santiago del Estero, Argentina, identified as Streptomyces sp. A14, able to remove and degrade MTX from liquid minimal medium. Thus, the purpose of this work is to contribute to the study of bioremediation of MTX by actinobacteria in contaminated soils. Glass pots were filled with 80 g of soil at 20% moisture. The soil samples were sterilized and the soil humidity was adjusted with sterile water and a MTX solution for different final concentrations (8.30 and 16.60 mg kg -1 wet weight (ww) soil). For soil samples inoculation, the microorganism was precultured in Tripticase Soya Broth with MTX (1.66 mg L-1). Soil samples pots with MTX and without MTX as control, were inoculated with Streptomyces sp. A14 (2 g kg-1 ww soil). Soil pots were incubated at 30 ºC for 28 days. Samples were taken each 7 days. The growth was measured as CFU kg-1 and residual MTX from soil was determined by gas chromatography. Our results indicate no significant differences in the growth at the different MTX concentrations added and in the control without MTX. The cell concentration increased up to 2 log units and the maximum growth of Streptomyces sp. A14 was 1.78 x 107 CFU g-1 ww soil, at 14 days of incubation. In both concentration assayed the microorganism was able to remove the soil pesticide, reaching the maximum removal percentages (36.14 and 76.02%) at 28 days of incubation. Finally, we suggest that Streptomyces sp. A14 has a big potential for bioremediation of soils contaminated with high MTX concentrations.
Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VII Congreso Argentino de Microbiologia General "Samige Del Bicentenario"
San Miguel de Tucumán
Argentina
Sociedad Argentina de Microbiología General
Materia
CONTAMINATED SOIL
METHOXYCHLOR
STREPTOMYCES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193034
Acceder
id CONICETDig_5a669e23905f70b7ee8428633d66b8c3
oai_identifier_str oai:ri.conicet.gov.ar:11336/193034
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14Bourguignon, NataliaCuozzo, Sergio AntonioFuentes, María SoledadBenimeli, Claudia SusanaAmoroso, Maria Julia del R.CONTAMINATED SOILMETHOXYCHLORSTREPTOMYCEShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Organochlorine pesticides (OP) have aroused global concern due to their long persistence, low biodegradability, wide range distribution in the environment and chronic adverse effect on wildlife and humans. Methoxychlor (MTX) is a toxic OP that was used in industrial and agricultural activities and for the malaria control. Although the use of MTX has been internationally banned it can still be detected in the environment like in the northwest of Argentina. Bioremediation offers the possibility to environment cleanup of pollutants, such as OP, using natural biological activity. However, there is little information available about microbial MTX degradation. Actinobacteria have a great potential for bioremediation of toxic compounds, in addition strains of Streptomyces genus may be well suited for soil inoculation as a consequence of their mycelial growth habit, relatively rapid rates of growth and colonization of semi-selective substrates. In our laboratory, we isolated an actinobacteria strain from OP-contaminated soil in Santiago del Estero, Argentina, identified as Streptomyces sp. A14, able to remove and degrade MTX from liquid minimal medium. Thus, the purpose of this work is to contribute to the study of bioremediation of MTX by actinobacteria in contaminated soils. Glass pots were filled with 80 g of soil at 20% moisture. The soil samples were sterilized and the soil humidity was adjusted with sterile water and a MTX solution for different final concentrations (8.30 and 16.60 mg kg -1 wet weight (ww) soil). For soil samples inoculation, the microorganism was precultured in Tripticase Soya Broth with MTX (1.66 mg L-1). Soil samples pots with MTX and without MTX as control, were inoculated with Streptomyces sp. A14 (2 g kg-1 ww soil). Soil pots were incubated at 30 ºC for 28 days. Samples were taken each 7 days. The growth was measured as CFU kg-1 and residual MTX from soil was determined by gas chromatography. Our results indicate no significant differences in the growth at the different MTX concentrations added and in the control without MTX. The cell concentration increased up to 2 log units and the maximum growth of Streptomyces sp. A14 was 1.78 x 107 CFU g-1 ww soil, at 14 days of incubation. In both concentration assayed the microorganism was able to remove the soil pesticide, reaching the maximum removal percentages (36.14 and 76.02%) at 28 days of incubation. Finally, we suggest that Streptomyces sp. A14 has a big potential for bioremediation of soils contaminated with high MTX concentrations.Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaVII Congreso Argentino de Microbiologia General "Samige Del Bicentenario"San Miguel de TucumánArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193034Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14; VII Congreso Argentino de Microbiologia General "Samige Del Bicentenario"; San Miguel de Tucumán; Argentina; 2011; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2011.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:03:14Zoai:ri.conicet.gov.ar:11336/193034instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:03:14.931CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14
title Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14
spellingShingle Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14
Bourguignon, Natalia
CONTAMINATED SOIL
METHOXYCHLOR
STREPTOMYCES
title_short Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14
title_full Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14
title_fullStr Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14
title_full_unstemmed Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14
title_sort Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14
dc.creator.none.fl_str_mv Bourguignon, Natalia
Cuozzo, Sergio Antonio
Fuentes, María Soledad
Benimeli, Claudia Susana
Amoroso, Maria Julia del R.
author Bourguignon, Natalia
author_facet Bourguignon, Natalia
Cuozzo, Sergio Antonio
Fuentes, María Soledad
Benimeli, Claudia Susana
Amoroso, Maria Julia del R.
author_role author
author2 Cuozzo, Sergio Antonio
Fuentes, María Soledad
Benimeli, Claudia Susana
Amoroso, Maria Julia del R.
author2_role author
author
author
author
dc.subject.none.fl_str_mv CONTAMINATED SOIL
METHOXYCHLOR
STREPTOMYCES
topic CONTAMINATED SOIL
METHOXYCHLOR
STREPTOMYCES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Organochlorine pesticides (OP) have aroused global concern due to their long persistence, low biodegradability, wide range distribution in the environment and chronic adverse effect on wildlife and humans. Methoxychlor (MTX) is a toxic OP that was used in industrial and agricultural activities and for the malaria control. Although the use of MTX has been internationally banned it can still be detected in the environment like in the northwest of Argentina. Bioremediation offers the possibility to environment cleanup of pollutants, such as OP, using natural biological activity. However, there is little information available about microbial MTX degradation. Actinobacteria have a great potential for bioremediation of toxic compounds, in addition strains of Streptomyces genus may be well suited for soil inoculation as a consequence of their mycelial growth habit, relatively rapid rates of growth and colonization of semi-selective substrates. In our laboratory, we isolated an actinobacteria strain from OP-contaminated soil in Santiago del Estero, Argentina, identified as Streptomyces sp. A14, able to remove and degrade MTX from liquid minimal medium. Thus, the purpose of this work is to contribute to the study of bioremediation of MTX by actinobacteria in contaminated soils. Glass pots were filled with 80 g of soil at 20% moisture. The soil samples were sterilized and the soil humidity was adjusted with sterile water and a MTX solution for different final concentrations (8.30 and 16.60 mg kg -1 wet weight (ww) soil). For soil samples inoculation, the microorganism was precultured in Tripticase Soya Broth with MTX (1.66 mg L-1). Soil samples pots with MTX and without MTX as control, were inoculated with Streptomyces sp. A14 (2 g kg-1 ww soil). Soil pots were incubated at 30 ºC for 28 days. Samples were taken each 7 days. The growth was measured as CFU kg-1 and residual MTX from soil was determined by gas chromatography. Our results indicate no significant differences in the growth at the different MTX concentrations added and in the control without MTX. The cell concentration increased up to 2 log units and the maximum growth of Streptomyces sp. A14 was 1.78 x 107 CFU g-1 ww soil, at 14 days of incubation. In both concentration assayed the microorganism was able to remove the soil pesticide, reaching the maximum removal percentages (36.14 and 76.02%) at 28 days of incubation. Finally, we suggest that Streptomyces sp. A14 has a big potential for bioremediation of soils contaminated with high MTX concentrations.
Fil: Bourguignon, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Amoroso, Maria Julia del R.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VII Congreso Argentino de Microbiologia General "Samige Del Bicentenario"
San Miguel de Tucumán
Argentina
Sociedad Argentina de Microbiología General
description Organochlorine pesticides (OP) have aroused global concern due to their long persistence, low biodegradability, wide range distribution in the environment and chronic adverse effect on wildlife and humans. Methoxychlor (MTX) is a toxic OP that was used in industrial and agricultural activities and for the malaria control. Although the use of MTX has been internationally banned it can still be detected in the environment like in the northwest of Argentina. Bioremediation offers the possibility to environment cleanup of pollutants, such as OP, using natural biological activity. However, there is little information available about microbial MTX degradation. Actinobacteria have a great potential for bioremediation of toxic compounds, in addition strains of Streptomyces genus may be well suited for soil inoculation as a consequence of their mycelial growth habit, relatively rapid rates of growth and colonization of semi-selective substrates. In our laboratory, we isolated an actinobacteria strain from OP-contaminated soil in Santiago del Estero, Argentina, identified as Streptomyces sp. A14, able to remove and degrade MTX from liquid minimal medium. Thus, the purpose of this work is to contribute to the study of bioremediation of MTX by actinobacteria in contaminated soils. Glass pots were filled with 80 g of soil at 20% moisture. The soil samples were sterilized and the soil humidity was adjusted with sterile water and a MTX solution for different final concentrations (8.30 and 16.60 mg kg -1 wet weight (ww) soil). For soil samples inoculation, the microorganism was precultured in Tripticase Soya Broth with MTX (1.66 mg L-1). Soil samples pots with MTX and without MTX as control, were inoculated with Streptomyces sp. A14 (2 g kg-1 ww soil). Soil pots were incubated at 30 ºC for 28 days. Samples were taken each 7 days. The growth was measured as CFU kg-1 and residual MTX from soil was determined by gas chromatography. Our results indicate no significant differences in the growth at the different MTX concentrations added and in the control without MTX. The cell concentration increased up to 2 log units and the maximum growth of Streptomyces sp. A14 was 1.78 x 107 CFU g-1 ww soil, at 14 days of incubation. In both concentration assayed the microorganism was able to remove the soil pesticide, reaching the maximum removal percentages (36.14 and 76.02%) at 28 days of incubation. Finally, we suggest that Streptomyces sp. A14 has a big potential for bioremediation of soils contaminated with high MTX concentrations.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193034
Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14; VII Congreso Argentino de Microbiologia General "Samige Del Bicentenario"; San Miguel de Tucumán; Argentina; 2011; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193034
identifier_str_mv Aerobic bioremediation of methoxychlor contaminated soil by Streptomyces sp. A14; VII Congreso Argentino de Microbiologia General "Samige Del Bicentenario"; San Miguel de Tucumán; Argentina; 2011; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2011.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842980069575753728
score 12.993085

Publicaciones similares