Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems

Autores
Fuentes, María Soledad; Sineli, Pedro Eugenio; Pons, Sofía; de Moreno, Maria Alejandra; Benimeli, Claudia Susana; Hill, Russell T.; Cuozzo, Sergio Antonio
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Currently, organochlorine pesticides (OPs) are restricted or banned from agricultural and public health areas. However, many of these compounds have been found in high concentrations in contaminated sites, and therefore continue to pose environmental and health concerns. Thus, it is imperative to identify efficient microorganisms to remove them and to develop methods to remediate environments contaminated with OPs. In this regard, the aim of this work was to evaluate the individual and simultaneous removal of three OPs; specifically lindane (LIN), γ-chlordane (CLD) and methoxychlor (MTX) (2 mg L-1 of each pesticide alone or as constituent of a mixture), from liquid contaminated systems, employing a native and non-GMO Streptomyces strain. Toxicity test of the treated systems against the cell line Caco-2, and the detection in the genome of the used microorganism of sequences related with the pesticides catabolism are reported. The results demonstrated that, Streptomyces sp. A5 was able to remove individually 57.4%, 100.0% and 6.5% of LIN, CLD and MTX, respectively. From the pesticide mixture, LIN and CLD were efficiently removed on 62.2% and 68.6% respectively by the studied strain. On the other hand, we demonstrated that the liquid systems contaminated with the OPs mixture and bioremediated with Streptomyces sp. A5 showed lower cytotoxicity than their respective controls without inoculate. Moreover, molecular studies revealed the presence in Streptomyces sp. A5 of putative lin genes with potential dehydrochlorinase, haloalkane dehalogenase and NAD-dependent dehydrogenase activities. Therefore, the potential of Streptomyces sp. A5 to efficiently remediate OPs alone or in mixtures from liquid contaminated system, was demonstrated.
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pons, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Hill, Russell T.. University of Maryland; Estados Unidos
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Materia
STREPTOMYCES
BIOREMEDIATION
PESTICIDES
GENES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/85524
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systemsFuentes, María SoledadSineli, Pedro EugenioPons, Sofíade Moreno, Maria AlejandraBenimeli, Claudia SusanaHill, Russell T.Cuozzo, Sergio AntonioSTREPTOMYCESBIOREMEDIATIONPESTICIDESGENEShttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2Currently, organochlorine pesticides (OPs) are restricted or banned from agricultural and public health areas. However, many of these compounds have been found in high concentrations in contaminated sites, and therefore continue to pose environmental and health concerns. Thus, it is imperative to identify efficient microorganisms to remove them and to develop methods to remediate environments contaminated with OPs. In this regard, the aim of this work was to evaluate the individual and simultaneous removal of three OPs; specifically lindane (LIN), γ-chlordane (CLD) and methoxychlor (MTX) (2 mg L-1 of each pesticide alone or as constituent of a mixture), from liquid contaminated systems, employing a native and non-GMO Streptomyces strain. Toxicity test of the treated systems against the cell line Caco-2, and the detection in the genome of the used microorganism of sequences related with the pesticides catabolism are reported. The results demonstrated that, Streptomyces sp. A5 was able to remove individually 57.4%, 100.0% and 6.5% of LIN, CLD and MTX, respectively. From the pesticide mixture, LIN and CLD were efficiently removed on 62.2% and 68.6% respectively by the studied strain. On the other hand, we demonstrated that the liquid systems contaminated with the OPs mixture and bioremediated with Streptomyces sp. A5 showed lower cytotoxicity than their respective controls without inoculate. Moreover, molecular studies revealed the presence in Streptomyces sp. A5 of putative lin genes with potential dehydrochlorinase, haloalkane dehalogenase and NAD-dependent dehydrogenase activities. Therefore, the potential of Streptomyces sp. A5 to efficiently remediate OPs alone or in mixtures from liquid contaminated system, was demonstrated.Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Pons, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Hill, Russell T.. University of Maryland; Estados UnidosFil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaELSEVIER2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/85524Fuentes, María Soledad; Sineli, Pedro Eugenio; Pons, Sofía; de Moreno, Maria Alejandra; Benimeli, Claudia Susana; et al.; Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems; ELSEVIER; Journal of Environmental Chemical Engineering; 6; 6; 12-2018; 6836-68432213-3437CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.jece.2018.10.023info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2213343718306286?via%3Dihubinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:48:13Zoai:ri.conicet.gov.ar:11336/85524instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:48:13.771CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems
title Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems
spellingShingle Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems
Fuentes, María Soledad
STREPTOMYCES
BIOREMEDIATION
PESTICIDES
GENES
title_short Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems
title_full Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems
title_fullStr Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems
title_full_unstemmed Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems
title_sort Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems
dc.creator.none.fl_str_mv Fuentes, María Soledad
Sineli, Pedro Eugenio
Pons, Sofía
de Moreno, Maria Alejandra
Benimeli, Claudia Susana
Hill, Russell T.
Cuozzo, Sergio Antonio
author Fuentes, María Soledad
author_facet Fuentes, María Soledad
Sineli, Pedro Eugenio
Pons, Sofía
de Moreno, Maria Alejandra
Benimeli, Claudia Susana
Hill, Russell T.
Cuozzo, Sergio Antonio
author_role author
author2 Sineli, Pedro Eugenio
Pons, Sofía
de Moreno, Maria Alejandra
Benimeli, Claudia Susana
Hill, Russell T.
Cuozzo, Sergio Antonio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv STREPTOMYCES
BIOREMEDIATION
PESTICIDES
GENES
topic STREPTOMYCES
BIOREMEDIATION
PESTICIDES
GENES
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Currently, organochlorine pesticides (OPs) are restricted or banned from agricultural and public health areas. However, many of these compounds have been found in high concentrations in contaminated sites, and therefore continue to pose environmental and health concerns. Thus, it is imperative to identify efficient microorganisms to remove them and to develop methods to remediate environments contaminated with OPs. In this regard, the aim of this work was to evaluate the individual and simultaneous removal of three OPs; specifically lindane (LIN), γ-chlordane (CLD) and methoxychlor (MTX) (2 mg L-1 of each pesticide alone or as constituent of a mixture), from liquid contaminated systems, employing a native and non-GMO Streptomyces strain. Toxicity test of the treated systems against the cell line Caco-2, and the detection in the genome of the used microorganism of sequences related with the pesticides catabolism are reported. The results demonstrated that, Streptomyces sp. A5 was able to remove individually 57.4%, 100.0% and 6.5% of LIN, CLD and MTX, respectively. From the pesticide mixture, LIN and CLD were efficiently removed on 62.2% and 68.6% respectively by the studied strain. On the other hand, we demonstrated that the liquid systems contaminated with the OPs mixture and bioremediated with Streptomyces sp. A5 showed lower cytotoxicity than their respective controls without inoculate. Moreover, molecular studies revealed the presence in Streptomyces sp. A5 of putative lin genes with potential dehydrochlorinase, haloalkane dehalogenase and NAD-dependent dehydrogenase activities. Therefore, the potential of Streptomyces sp. A5 to efficiently remediate OPs alone or in mixtures from liquid contaminated system, was demonstrated.
Fil: Fuentes, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Sineli, Pedro Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Pons, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Benimeli, Claudia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Hill, Russell T.. University of Maryland; Estados Unidos
Fil: Cuozzo, Sergio Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
description Currently, organochlorine pesticides (OPs) are restricted or banned from agricultural and public health areas. However, many of these compounds have been found in high concentrations in contaminated sites, and therefore continue to pose environmental and health concerns. Thus, it is imperative to identify efficient microorganisms to remove them and to develop methods to remediate environments contaminated with OPs. In this regard, the aim of this work was to evaluate the individual and simultaneous removal of three OPs; specifically lindane (LIN), γ-chlordane (CLD) and methoxychlor (MTX) (2 mg L-1 of each pesticide alone or as constituent of a mixture), from liquid contaminated systems, employing a native and non-GMO Streptomyces strain. Toxicity test of the treated systems against the cell line Caco-2, and the detection in the genome of the used microorganism of sequences related with the pesticides catabolism are reported. The results demonstrated that, Streptomyces sp. A5 was able to remove individually 57.4%, 100.0% and 6.5% of LIN, CLD and MTX, respectively. From the pesticide mixture, LIN and CLD were efficiently removed on 62.2% and 68.6% respectively by the studied strain. On the other hand, we demonstrated that the liquid systems contaminated with the OPs mixture and bioremediated with Streptomyces sp. A5 showed lower cytotoxicity than their respective controls without inoculate. Moreover, molecular studies revealed the presence in Streptomyces sp. A5 of putative lin genes with potential dehydrochlorinase, haloalkane dehalogenase and NAD-dependent dehydrogenase activities. Therefore, the potential of Streptomyces sp. A5 to efficiently remediate OPs alone or in mixtures from liquid contaminated system, was demonstrated.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/85524
Fuentes, María Soledad; Sineli, Pedro Eugenio; Pons, Sofía; de Moreno, Maria Alejandra; Benimeli, Claudia Susana; et al.; Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems; ELSEVIER; Journal of Environmental Chemical Engineering; 6; 6; 12-2018; 6836-6843
2213-3437
CONICET Digital
CONICET
url http://hdl.handle.net/11336/85524
identifier_str_mv Fuentes, María Soledad; Sineli, Pedro Eugenio; Pons, Sofía; de Moreno, Maria Alejandra; Benimeli, Claudia Susana; et al.; Study of the removal of a pesticides mixture by a Streptomyces strain and their effect on the cytotoxicity of treated systems; ELSEVIER; Journal of Environmental Chemical Engineering; 6; 6; 12-2018; 6836-6843
2213-3437
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://doi.org/10.1016/j.jece.2018.10.023
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S2213343718306286?via%3Dihub
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.976206

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