Characterization of viral particles isolated from primary cultures of human breast cancer cells

Autores
Melana, Stella M.; Nepomnaschy, Irene; Sakalian, Michael; Abbott, Andrea; Hasa, Jennifer; Holland, James F.; Pogo, Beatriz G.T.
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The association of human breast cancer with sequences similar to the mouse mammary tumor virus (MMTV) has been shown, but convincing evidence for the presence of viral particles in breast tumors has been lacking. We have described the complete proviral structure of a retrovirus in human breast cancer. This provirus, designated as human mammary tumor virus (HMTV), was 95% homologous to MMTV and revealed features of a replication-competent virus. We have therefore investigated the production of viral particles in primary cultures of human breast cancer (MSSM). Cells isolated from ascites or pleural effusions of patients with metastatic breast cancer contained viral sequences in their DNA, expressed Env protein, and showed retroviral particles by electron microscopy. Viral particles from culture media exhibited morphologic features of β-retroviruses sedimenting at buoyant densities of 1.12 to 1.18 g/mL in sucrose gradients and showed reverse transcriptase activity. cDNA sequences from virion RNA were synthesized, amplified, and sequenced and all the virion genes were detected and 70% of the virion RNA was sequenced. The sequence homologies were, respectively, 85% to 95% compared with the MMTV and HMTV proviruses we have previously described. These results clearly show that breast cancer cells in primary cultures produced HMTV viral particles that are similar to the mouse virus and which may play a role in human breast cancer pathogenesis. ©2007 American Association for Cancer Research.
Fil: Melana, Stella M.. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Nepomnaschy, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Sakalian, Michael. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Abbott, Andrea. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Hasa, Jennifer. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Holland, James F.. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Pogo, Beatriz G.T.. University of Oklahoma Health Sciences Center; Estados Unidos
Materia
Hmtv
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55456
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/55456
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Characterization of viral particles isolated from primary cultures of human breast cancer cellsMelana, Stella M.Nepomnaschy, IreneSakalian, MichaelAbbott, AndreaHasa, JenniferHolland, James F.Pogo, Beatriz G.T.Hmtvhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The association of human breast cancer with sequences similar to the mouse mammary tumor virus (MMTV) has been shown, but convincing evidence for the presence of viral particles in breast tumors has been lacking. We have described the complete proviral structure of a retrovirus in human breast cancer. This provirus, designated as human mammary tumor virus (HMTV), was 95% homologous to MMTV and revealed features of a replication-competent virus. We have therefore investigated the production of viral particles in primary cultures of human breast cancer (MSSM). Cells isolated from ascites or pleural effusions of patients with metastatic breast cancer contained viral sequences in their DNA, expressed Env protein, and showed retroviral particles by electron microscopy. Viral particles from culture media exhibited morphologic features of β-retroviruses sedimenting at buoyant densities of 1.12 to 1.18 g/mL in sucrose gradients and showed reverse transcriptase activity. cDNA sequences from virion RNA were synthesized, amplified, and sequenced and all the virion genes were detected and 70% of the virion RNA was sequenced. The sequence homologies were, respectively, 85% to 95% compared with the MMTV and HMTV proviruses we have previously described. These results clearly show that breast cancer cells in primary cultures produced HMTV viral particles that are similar to the mouse virus and which may play a role in human breast cancer pathogenesis. ©2007 American Association for Cancer Research.Fil: Melana, Stella M.. University of Oklahoma Health Sciences Center; Estados UnidosFil: Nepomnaschy, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Oklahoma Health Sciences Center; Estados UnidosFil: Sakalian, Michael. University of Oklahoma Health Sciences Center; Estados UnidosFil: Abbott, Andrea. University of Oklahoma Health Sciences Center; Estados UnidosFil: Hasa, Jennifer. University of Oklahoma Health Sciences Center; Estados UnidosFil: Holland, James F.. University of Oklahoma Health Sciences Center; Estados UnidosFil: Pogo, Beatriz G.T.. University of Oklahoma Health Sciences Center; Estados UnidosAmerican Association for Cancer Research2007-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55456Melana, Stella M.; Nepomnaschy, Irene; Sakalian, Michael; Abbott, Andrea; Hasa, Jennifer; et al.; Characterization of viral particles isolated from primary cultures of human breast cancer cells; American Association for Cancer Research; Cancer Research; 67; 18; 9-2007; 8960-89650008-5472CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1158/0008-5472.CAN-06-3892info:eu-repo/semantics/altIdentifier/url/http://cancerres.aacrjournals.org/content/67/18/8960info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:50:47Zoai:ri.conicet.gov.ar:11336/55456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:50:48.204CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Characterization of viral particles isolated from primary cultures of human breast cancer cells
title Characterization of viral particles isolated from primary cultures of human breast cancer cells
spellingShingle Characterization of viral particles isolated from primary cultures of human breast cancer cells
Melana, Stella M.
Hmtv
title_short Characterization of viral particles isolated from primary cultures of human breast cancer cells
title_full Characterization of viral particles isolated from primary cultures of human breast cancer cells
title_fullStr Characterization of viral particles isolated from primary cultures of human breast cancer cells
title_full_unstemmed Characterization of viral particles isolated from primary cultures of human breast cancer cells
title_sort Characterization of viral particles isolated from primary cultures of human breast cancer cells
dc.creator.none.fl_str_mv Melana, Stella M.
Nepomnaschy, Irene
Sakalian, Michael
Abbott, Andrea
Hasa, Jennifer
Holland, James F.
Pogo, Beatriz G.T.
author Melana, Stella M.
author_facet Melana, Stella M.
Nepomnaschy, Irene
Sakalian, Michael
Abbott, Andrea
Hasa, Jennifer
Holland, James F.
Pogo, Beatriz G.T.
author_role author
author2 Nepomnaschy, Irene
Sakalian, Michael
Abbott, Andrea
Hasa, Jennifer
Holland, James F.
Pogo, Beatriz G.T.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Hmtv
topic Hmtv
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The association of human breast cancer with sequences similar to the mouse mammary tumor virus (MMTV) has been shown, but convincing evidence for the presence of viral particles in breast tumors has been lacking. We have described the complete proviral structure of a retrovirus in human breast cancer. This provirus, designated as human mammary tumor virus (HMTV), was 95% homologous to MMTV and revealed features of a replication-competent virus. We have therefore investigated the production of viral particles in primary cultures of human breast cancer (MSSM). Cells isolated from ascites or pleural effusions of patients with metastatic breast cancer contained viral sequences in their DNA, expressed Env protein, and showed retroviral particles by electron microscopy. Viral particles from culture media exhibited morphologic features of β-retroviruses sedimenting at buoyant densities of 1.12 to 1.18 g/mL in sucrose gradients and showed reverse transcriptase activity. cDNA sequences from virion RNA were synthesized, amplified, and sequenced and all the virion genes were detected and 70% of the virion RNA was sequenced. The sequence homologies were, respectively, 85% to 95% compared with the MMTV and HMTV proviruses we have previously described. These results clearly show that breast cancer cells in primary cultures produced HMTV viral particles that are similar to the mouse virus and which may play a role in human breast cancer pathogenesis. ©2007 American Association for Cancer Research.
Fil: Melana, Stella M.. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Nepomnaschy, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Sakalian, Michael. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Abbott, Andrea. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Hasa, Jennifer. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Holland, James F.. University of Oklahoma Health Sciences Center; Estados Unidos
Fil: Pogo, Beatriz G.T.. University of Oklahoma Health Sciences Center; Estados Unidos
description The association of human breast cancer with sequences similar to the mouse mammary tumor virus (MMTV) has been shown, but convincing evidence for the presence of viral particles in breast tumors has been lacking. We have described the complete proviral structure of a retrovirus in human breast cancer. This provirus, designated as human mammary tumor virus (HMTV), was 95% homologous to MMTV and revealed features of a replication-competent virus. We have therefore investigated the production of viral particles in primary cultures of human breast cancer (MSSM). Cells isolated from ascites or pleural effusions of patients with metastatic breast cancer contained viral sequences in their DNA, expressed Env protein, and showed retroviral particles by electron microscopy. Viral particles from culture media exhibited morphologic features of β-retroviruses sedimenting at buoyant densities of 1.12 to 1.18 g/mL in sucrose gradients and showed reverse transcriptase activity. cDNA sequences from virion RNA were synthesized, amplified, and sequenced and all the virion genes were detected and 70% of the virion RNA was sequenced. The sequence homologies were, respectively, 85% to 95% compared with the MMTV and HMTV proviruses we have previously described. These results clearly show that breast cancer cells in primary cultures produced HMTV viral particles that are similar to the mouse virus and which may play a role in human breast cancer pathogenesis. ©2007 American Association for Cancer Research.
publishDate 2007
dc.date.none.fl_str_mv 2007-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55456
Melana, Stella M.; Nepomnaschy, Irene; Sakalian, Michael; Abbott, Andrea; Hasa, Jennifer; et al.; Characterization of viral particles isolated from primary cultures of human breast cancer cells; American Association for Cancer Research; Cancer Research; 67; 18; 9-2007; 8960-8965
0008-5472
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55456
identifier_str_mv Melana, Stella M.; Nepomnaschy, Irene; Sakalian, Michael; Abbott, Andrea; Hasa, Jennifer; et al.; Characterization of viral particles isolated from primary cultures of human breast cancer cells; American Association for Cancer Research; Cancer Research; 67; 18; 9-2007; 8960-8965
0008-5472
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1158/0008-5472.CAN-06-3892
info:eu-repo/semantics/altIdentifier/url/http://cancerres.aacrjournals.org/content/67/18/8960
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association for Cancer Research
publisher.none.fl_str_mv American Association for Cancer Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083033051955200
score 13.22299

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