Detection of human mammary tumor virus proteins in human breast cancer cells

Autores
Melana, Stella M.; Nepomnaschy, Irene; Hasa, Jennifer; Djougarian, Alina; Djougarian, Anna; Holland, James F.; Pogo, Beatriz G.T.
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mouse mammary tumor virus (MMTV) has been proven to induce mammary cancer in mice. MMTV-like env gene sequences have been detected in one-third of the human breast tumors studied. The whole proviral structure with 95% homology to MMTV was found in two human breast tumors and was designated as human mammary tumor virus (HMTV). HMTV viral particles with betaretroviral features have been isolated. In addition, a retrovirus called human betaretrovirus (HBRV), homologous to the mentioned retroviruses, has been isolated from tissues of patients with primary biliary cirrhosis. In this report, the expression of HMTV envelope (Env) and capsid (Ca) was detected in 10 primary cultures of human breast cancer containing HMTV sequences (MSSM) by Western blot and fluorescence activated cell sorting (FACS), using a panel of antibodies against HMTV Env, HBRV Env and Ca and the MMTV Env Gp36 and Ca P27 proteins. By contrast, HMTV proteins did not react with antibody against the MMTV Env Gp52 protein. All the antibodies detected MMTV proteins with exception of two out of four monoclonal antibodies against HMTV Env. Approximately 13% of the MSSM cells showed HMTV protein expression by FACS analysis. This report shows the expression of HMTV proteins for the first time in human breast cancer cells using a panel of antibodies against HMTV, HBRV and MMTV proteins. This should be taken into consideration when MMTV antibodies are used to detect HMTV proteins in human tissues. © 2009 Elsevier B.V. All rights reserved.
Fil: Melana, Stella M.. Mount Sinai School of Medicine; Estados Unidos
Fil: Nepomnaschy, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Mount Sinai School of Medicine; Estados Unidos
Fil: Hasa, Jennifer. Mount Sinai School of Medicine; Estados Unidos
Fil: Djougarian, Alina. Mount Sinai School of Medicine; Estados Unidos
Fil: Djougarian, Anna. Mount Sinai School of Medicine; Estados Unidos
Fil: Holland, James F.. Mount Sinai School of Medicine; Estados Unidos
Fil: Pogo, Beatriz G.T.. Mount Sinai School of Medicine; Estados Unidos
Materia
Human Breast Cancer
Human Mammary Tumor Virus
Retroviral Proteins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/53614
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of human mammary tumor virus proteins in human breast cancer cellsMelana, Stella M.Nepomnaschy, IreneHasa, JenniferDjougarian, AlinaDjougarian, AnnaHolland, James F.Pogo, Beatriz G.T.Human Breast CancerHuman Mammary Tumor VirusRetroviral Proteinshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Mouse mammary tumor virus (MMTV) has been proven to induce mammary cancer in mice. MMTV-like env gene sequences have been detected in one-third of the human breast tumors studied. The whole proviral structure with 95% homology to MMTV was found in two human breast tumors and was designated as human mammary tumor virus (HMTV). HMTV viral particles with betaretroviral features have been isolated. In addition, a retrovirus called human betaretrovirus (HBRV), homologous to the mentioned retroviruses, has been isolated from tissues of patients with primary biliary cirrhosis. In this report, the expression of HMTV envelope (Env) and capsid (Ca) was detected in 10 primary cultures of human breast cancer containing HMTV sequences (MSSM) by Western blot and fluorescence activated cell sorting (FACS), using a panel of antibodies against HMTV Env, HBRV Env and Ca and the MMTV Env Gp36 and Ca P27 proteins. By contrast, HMTV proteins did not react with antibody against the MMTV Env Gp52 protein. All the antibodies detected MMTV proteins with exception of two out of four monoclonal antibodies against HMTV Env. Approximately 13% of the MSSM cells showed HMTV protein expression by FACS analysis. This report shows the expression of HMTV proteins for the first time in human breast cancer cells using a panel of antibodies against HMTV, HBRV and MMTV proteins. This should be taken into consideration when MMTV antibodies are used to detect HMTV proteins in human tissues. © 2009 Elsevier B.V. All rights reserved.Fil: Melana, Stella M.. Mount Sinai School of Medicine; Estados UnidosFil: Nepomnaschy, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Mount Sinai School of Medicine; Estados UnidosFil: Hasa, Jennifer. Mount Sinai School of Medicine; Estados UnidosFil: Djougarian, Alina. Mount Sinai School of Medicine; Estados UnidosFil: Djougarian, Anna. Mount Sinai School of Medicine; Estados UnidosFil: Holland, James F.. Mount Sinai School of Medicine; Estados UnidosFil: Pogo, Beatriz G.T.. Mount Sinai School of Medicine; Estados UnidosElsevier Science2010-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/53614Melana, Stella M.; Nepomnaschy, Irene; Hasa, Jennifer; Djougarian, Alina; Djougarian, Anna; et al.; Detection of human mammary tumor virus proteins in human breast cancer cells; Elsevier Science; Journal of Virological Methods; 163; 1; 1-2010; 157-1610166-0934CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2009.09.015info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166093409004157info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:42Zoai:ri.conicet.gov.ar:11336/53614instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:42.617CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of human mammary tumor virus proteins in human breast cancer cells
title Detection of human mammary tumor virus proteins in human breast cancer cells
spellingShingle Detection of human mammary tumor virus proteins in human breast cancer cells
Melana, Stella M.
Human Breast Cancer
Human Mammary Tumor Virus
Retroviral Proteins
title_short Detection of human mammary tumor virus proteins in human breast cancer cells
title_full Detection of human mammary tumor virus proteins in human breast cancer cells
title_fullStr Detection of human mammary tumor virus proteins in human breast cancer cells
title_full_unstemmed Detection of human mammary tumor virus proteins in human breast cancer cells
title_sort Detection of human mammary tumor virus proteins in human breast cancer cells
dc.creator.none.fl_str_mv Melana, Stella M.
Nepomnaschy, Irene
Hasa, Jennifer
Djougarian, Alina
Djougarian, Anna
Holland, James F.
Pogo, Beatriz G.T.
author Melana, Stella M.
author_facet Melana, Stella M.
Nepomnaschy, Irene
Hasa, Jennifer
Djougarian, Alina
Djougarian, Anna
Holland, James F.
Pogo, Beatriz G.T.
author_role author
author2 Nepomnaschy, Irene
Hasa, Jennifer
Djougarian, Alina
Djougarian, Anna
Holland, James F.
Pogo, Beatriz G.T.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Human Breast Cancer
Human Mammary Tumor Virus
Retroviral Proteins
topic Human Breast Cancer
Human Mammary Tumor Virus
Retroviral Proteins
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Mouse mammary tumor virus (MMTV) has been proven to induce mammary cancer in mice. MMTV-like env gene sequences have been detected in one-third of the human breast tumors studied. The whole proviral structure with 95% homology to MMTV was found in two human breast tumors and was designated as human mammary tumor virus (HMTV). HMTV viral particles with betaretroviral features have been isolated. In addition, a retrovirus called human betaretrovirus (HBRV), homologous to the mentioned retroviruses, has been isolated from tissues of patients with primary biliary cirrhosis. In this report, the expression of HMTV envelope (Env) and capsid (Ca) was detected in 10 primary cultures of human breast cancer containing HMTV sequences (MSSM) by Western blot and fluorescence activated cell sorting (FACS), using a panel of antibodies against HMTV Env, HBRV Env and Ca and the MMTV Env Gp36 and Ca P27 proteins. By contrast, HMTV proteins did not react with antibody against the MMTV Env Gp52 protein. All the antibodies detected MMTV proteins with exception of two out of four monoclonal antibodies against HMTV Env. Approximately 13% of the MSSM cells showed HMTV protein expression by FACS analysis. This report shows the expression of HMTV proteins for the first time in human breast cancer cells using a panel of antibodies against HMTV, HBRV and MMTV proteins. This should be taken into consideration when MMTV antibodies are used to detect HMTV proteins in human tissues. © 2009 Elsevier B.V. All rights reserved.
Fil: Melana, Stella M.. Mount Sinai School of Medicine; Estados Unidos
Fil: Nepomnaschy, Irene. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Mount Sinai School of Medicine; Estados Unidos
Fil: Hasa, Jennifer. Mount Sinai School of Medicine; Estados Unidos
Fil: Djougarian, Alina. Mount Sinai School of Medicine; Estados Unidos
Fil: Djougarian, Anna. Mount Sinai School of Medicine; Estados Unidos
Fil: Holland, James F.. Mount Sinai School of Medicine; Estados Unidos
Fil: Pogo, Beatriz G.T.. Mount Sinai School of Medicine; Estados Unidos
description Mouse mammary tumor virus (MMTV) has been proven to induce mammary cancer in mice. MMTV-like env gene sequences have been detected in one-third of the human breast tumors studied. The whole proviral structure with 95% homology to MMTV was found in two human breast tumors and was designated as human mammary tumor virus (HMTV). HMTV viral particles with betaretroviral features have been isolated. In addition, a retrovirus called human betaretrovirus (HBRV), homologous to the mentioned retroviruses, has been isolated from tissues of patients with primary biliary cirrhosis. In this report, the expression of HMTV envelope (Env) and capsid (Ca) was detected in 10 primary cultures of human breast cancer containing HMTV sequences (MSSM) by Western blot and fluorescence activated cell sorting (FACS), using a panel of antibodies against HMTV Env, HBRV Env and Ca and the MMTV Env Gp36 and Ca P27 proteins. By contrast, HMTV proteins did not react with antibody against the MMTV Env Gp52 protein. All the antibodies detected MMTV proteins with exception of two out of four monoclonal antibodies against HMTV Env. Approximately 13% of the MSSM cells showed HMTV protein expression by FACS analysis. This report shows the expression of HMTV proteins for the first time in human breast cancer cells using a panel of antibodies against HMTV, HBRV and MMTV proteins. This should be taken into consideration when MMTV antibodies are used to detect HMTV proteins in human tissues. © 2009 Elsevier B.V. All rights reserved.
publishDate 2010
dc.date.none.fl_str_mv 2010-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/53614
Melana, Stella M.; Nepomnaschy, Irene; Hasa, Jennifer; Djougarian, Alina; Djougarian, Anna; et al.; Detection of human mammary tumor virus proteins in human breast cancer cells; Elsevier Science; Journal of Virological Methods; 163; 1; 1-2010; 157-161
0166-0934
CONICET Digital
CONICET
url http://hdl.handle.net/11336/53614
identifier_str_mv Melana, Stella M.; Nepomnaschy, Irene; Hasa, Jennifer; Djougarian, Alina; Djougarian, Anna; et al.; Detection of human mammary tumor virus proteins in human breast cancer cells; Elsevier Science; Journal of Virological Methods; 163; 1; 1-2010; 157-161
0166-0934
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jviromet.2009.09.015
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166093409004157
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268989121626112
score 13.13397

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