Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry

Autores
Dominguez, Gabriela Alejandra; Perlo, Josefina; Fraenza, Carla Cecilia; Anoardo, Esteban
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The elastic properties of lipid membranes can be conveniently characterized through the bending elastic modulus κ. Elasticity directly affects the deformability of a membrane, morphological and shape transitions, fusion, lipid-protein interactions, etc. It is also a critical property for the formulation of ultradeformable liposomes, and of interest for the design of theranostic liposomes for efficient drug delivery systems and/or different imaging contrast agents. Measurements of κ in liposome membranes have been made using the fast field cycling nuclear magnetic relaxometry technique. We analyze the capability of the technique to provide a consistent value of the measured quantity under certain limiting conditions. Relaxation dispersions were measured acquiring a minimal quantity of points, within a reduced Larmor frequency range and, under inferior experimental conditions (in the presence of magnetic field in-homogeneity and lower power supply stability). A simplified model is discussed, showing practical advantages when fitting the data within the reduced frequency range. Experiments are contrasted with standard measurements performed in a state-of-the-art relaxometer. The methodology was tested in samples of 1,2-dimyristoyl-sn-glycero-3-phosphocholine with different percentiles of cholesterol. We observe a tendency to a decrease in κ with increasing temperature, and a tendency to increase with the cholesterol percentile.
Fil: Dominguez, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Perlo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Fraenza, Carla Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Anoardo, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Materia
BENDING MODULUS
LIPID DYNAMICS
LIPOSOMES
NMR RELAXOMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/186033
Acceder
id CONICETDig_fcaf1ecfacccccacac6d88e2eabd2918
oai_identifier_str oai:ri.conicet.gov.ar:11336/186033
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometryDominguez, Gabriela AlejandraPerlo, JosefinaFraenza, Carla CeciliaAnoardo, EstebanBENDING MODULUSLIPID DYNAMICSLIPOSOMESNMR RELAXOMETRYhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The elastic properties of lipid membranes can be conveniently characterized through the bending elastic modulus κ. Elasticity directly affects the deformability of a membrane, morphological and shape transitions, fusion, lipid-protein interactions, etc. It is also a critical property for the formulation of ultradeformable liposomes, and of interest for the design of theranostic liposomes for efficient drug delivery systems and/or different imaging contrast agents. Measurements of κ in liposome membranes have been made using the fast field cycling nuclear magnetic relaxometry technique. We analyze the capability of the technique to provide a consistent value of the measured quantity under certain limiting conditions. Relaxation dispersions were measured acquiring a minimal quantity of points, within a reduced Larmor frequency range and, under inferior experimental conditions (in the presence of magnetic field in-homogeneity and lower power supply stability). A simplified model is discussed, showing practical advantages when fitting the data within the reduced frequency range. Experiments are contrasted with standard measurements performed in a state-of-the-art relaxometer. The methodology was tested in samples of 1,2-dimyristoyl-sn-glycero-3-phosphocholine with different percentiles of cholesterol. We observe a tendency to a decrease in κ with increasing temperature, and a tendency to increase with the cholesterol percentile.Fil: Dominguez, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Perlo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Fraenza, Carla Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Anoardo, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaElsevier Ireland2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/186033Dominguez, Gabriela Alejandra; Perlo, Josefina; Fraenza, Carla Cecilia; Anoardo, Esteban; Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry; Elsevier Ireland; Chemistry and Physics of Lipids; 201; 12-2016; 21-270009-3084CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0009308416301505info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2016.10.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:40:27Zoai:ri.conicet.gov.ar:11336/186033instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:40:27.69CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry
title Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry
spellingShingle Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry
Dominguez, Gabriela Alejandra
BENDING MODULUS
LIPID DYNAMICS
LIPOSOMES
NMR RELAXOMETRY
title_short Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry
title_full Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry
title_fullStr Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry
title_full_unstemmed Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry
title_sort Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry
dc.creator.none.fl_str_mv Dominguez, Gabriela Alejandra
Perlo, Josefina
Fraenza, Carla Cecilia
Anoardo, Esteban
author Dominguez, Gabriela Alejandra
author_facet Dominguez, Gabriela Alejandra
Perlo, Josefina
Fraenza, Carla Cecilia
Anoardo, Esteban
author_role author
author2 Perlo, Josefina
Fraenza, Carla Cecilia
Anoardo, Esteban
author2_role author
author
author
dc.subject.none.fl_str_mv BENDING MODULUS
LIPID DYNAMICS
LIPOSOMES
NMR RELAXOMETRY
topic BENDING MODULUS
LIPID DYNAMICS
LIPOSOMES
NMR RELAXOMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The elastic properties of lipid membranes can be conveniently characterized through the bending elastic modulus κ. Elasticity directly affects the deformability of a membrane, morphological and shape transitions, fusion, lipid-protein interactions, etc. It is also a critical property for the formulation of ultradeformable liposomes, and of interest for the design of theranostic liposomes for efficient drug delivery systems and/or different imaging contrast agents. Measurements of κ in liposome membranes have been made using the fast field cycling nuclear magnetic relaxometry technique. We analyze the capability of the technique to provide a consistent value of the measured quantity under certain limiting conditions. Relaxation dispersions were measured acquiring a minimal quantity of points, within a reduced Larmor frequency range and, under inferior experimental conditions (in the presence of magnetic field in-homogeneity and lower power supply stability). A simplified model is discussed, showing practical advantages when fitting the data within the reduced frequency range. Experiments are contrasted with standard measurements performed in a state-of-the-art relaxometer. The methodology was tested in samples of 1,2-dimyristoyl-sn-glycero-3-phosphocholine with different percentiles of cholesterol. We observe a tendency to a decrease in κ with increasing temperature, and a tendency to increase with the cholesterol percentile.
Fil: Dominguez, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Perlo, Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Fraenza, Carla Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Anoardo, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
description The elastic properties of lipid membranes can be conveniently characterized through the bending elastic modulus κ. Elasticity directly affects the deformability of a membrane, morphological and shape transitions, fusion, lipid-protein interactions, etc. It is also a critical property for the formulation of ultradeformable liposomes, and of interest for the design of theranostic liposomes for efficient drug delivery systems and/or different imaging contrast agents. Measurements of κ in liposome membranes have been made using the fast field cycling nuclear magnetic relaxometry technique. We analyze the capability of the technique to provide a consistent value of the measured quantity under certain limiting conditions. Relaxation dispersions were measured acquiring a minimal quantity of points, within a reduced Larmor frequency range and, under inferior experimental conditions (in the presence of magnetic field in-homogeneity and lower power supply stability). A simplified model is discussed, showing practical advantages when fitting the data within the reduced frequency range. Experiments are contrasted with standard measurements performed in a state-of-the-art relaxometer. The methodology was tested in samples of 1,2-dimyristoyl-sn-glycero-3-phosphocholine with different percentiles of cholesterol. We observe a tendency to a decrease in κ with increasing temperature, and a tendency to increase with the cholesterol percentile.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/186033
Dominguez, Gabriela Alejandra; Perlo, Josefina; Fraenza, Carla Cecilia; Anoardo, Esteban; Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry; Elsevier Ireland; Chemistry and Physics of Lipids; 201; 12-2016; 21-27
0009-3084
CONICET Digital
CONICET
url http://hdl.handle.net/11336/186033
identifier_str_mv Dominguez, Gabriela Alejandra; Perlo, Josefina; Fraenza, Carla Cecilia; Anoardo, Esteban; Measurements of the bending elastic modulus in unilamellar vesicles membranes by fast field cycling NMR relaxometry; Elsevier Ireland; Chemistry and Physics of Lipids; 201; 12-2016; 21-27
0009-3084
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0009308416301505
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.chemphyslip.2016.10.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.142177

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