The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles

Autores
Fraenza, Carla Cecilia; Meledandri, Carla J.; Anoardo, Esteban; Brougham, Dermot F.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The general applicability of fast field-cycling nuclear magnetic resonance relaxometry in the study of dynamics in lipid bilayers is demonstrated through analysis of binary unilamellar liposomes composed of 1,2-dioleoyl-sn-glycero-3-posphocholine (DOPC) and cholesterol. We extend an evidence-based method to simulating the NMR relaxation response, previously validated for single-component membranes, to evaluate the effect of the sterol molecule on local ordering and dynamics over multiple timescales. The relaxometric results are found to be most consistent with the partitioning of the lipid molecules into affected and unaffected portions, rather than a single averaged phase. Our analysis suggests that up to 25 mol%, each cholesterol molecule orders three DOPC molecules, providing experimental backup to the findings of many molecular dynamics studies. A methodology is established for studying dynamics on multiple timescales in unilamellar membranes of more complex compositions.
Fil: Fraenza, Carla Cecilia. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Meledandri, Carla J.. Universidad de Dublin; Irlanda. University of Otago; Nueva Zelanda
Fil: Anoardo, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Brougham, Dermot F.. Universidad de Dublin; Irlanda
Materia
Cholesterol Effect
Fast Field-Cycling Nmr
Lipid Bilayer
Molecular Dynamics
Nmr Spectroscopy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/31844
Acceder
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network_name_str CONICET Digital (CONICET)
spelling The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar VesiclesFraenza, Carla CeciliaMeledandri, Carla J.Anoardo, EstebanBrougham, Dermot F.Cholesterol EffectFast Field-Cycling NmrLipid BilayerMolecular DynamicsNmr Spectroscopyhttps://purl.org/becyt/ford/1.3https://purl.org/becyt/ford/1The general applicability of fast field-cycling nuclear magnetic resonance relaxometry in the study of dynamics in lipid bilayers is demonstrated through analysis of binary unilamellar liposomes composed of 1,2-dioleoyl-sn-glycero-3-posphocholine (DOPC) and cholesterol. We extend an evidence-based method to simulating the NMR relaxation response, previously validated for single-component membranes, to evaluate the effect of the sterol molecule on local ordering and dynamics over multiple timescales. The relaxometric results are found to be most consistent with the partitioning of the lipid molecules into affected and unaffected portions, rather than a single averaged phase. Our analysis suggests that up to 25 mol%, each cholesterol molecule orders three DOPC molecules, providing experimental backup to the findings of many molecular dynamics studies. A methodology is established for studying dynamics on multiple timescales in unilamellar membranes of more complex compositions.Fil: Fraenza, Carla Cecilia. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; ArgentinaFil: Meledandri, Carla J.. Universidad de Dublin; Irlanda. University of Otago; Nueva ZelandaFil: Anoardo, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; ArgentinaFil: Brougham, Dermot F.. Universidad de Dublin; IrlandaWiley VCH Verlag2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/31844Brougham, Dermot F.; Anoardo, Esteban; Meledandri, Carla J.; Fraenza, Carla Cecilia; The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles; Wiley VCH Verlag; Chemphyschem; 15; 3; 1-2014; 425-4351439-4235CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/cphc.201301051info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cphc.201301051/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:19:58Zoai:ri.conicet.gov.ar:11336/31844instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:19:58.24CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles
title The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles
spellingShingle The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles
Fraenza, Carla Cecilia
Cholesterol Effect
Fast Field-Cycling Nmr
Lipid Bilayer
Molecular Dynamics
Nmr Spectroscopy
title_short The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles
title_full The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles
title_fullStr The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles
title_full_unstemmed The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles
title_sort The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles
dc.creator.none.fl_str_mv Fraenza, Carla Cecilia
Meledandri, Carla J.
Anoardo, Esteban
Brougham, Dermot F.
author Fraenza, Carla Cecilia
author_facet Fraenza, Carla Cecilia
Meledandri, Carla J.
Anoardo, Esteban
Brougham, Dermot F.
author_role author
author2 Meledandri, Carla J.
Anoardo, Esteban
Brougham, Dermot F.
author2_role author
author
author
dc.subject.none.fl_str_mv Cholesterol Effect
Fast Field-Cycling Nmr
Lipid Bilayer
Molecular Dynamics
Nmr Spectroscopy
topic Cholesterol Effect
Fast Field-Cycling Nmr
Lipid Bilayer
Molecular Dynamics
Nmr Spectroscopy
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.3
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The general applicability of fast field-cycling nuclear magnetic resonance relaxometry in the study of dynamics in lipid bilayers is demonstrated through analysis of binary unilamellar liposomes composed of 1,2-dioleoyl-sn-glycero-3-posphocholine (DOPC) and cholesterol. We extend an evidence-based method to simulating the NMR relaxation response, previously validated for single-component membranes, to evaluate the effect of the sterol molecule on local ordering and dynamics over multiple timescales. The relaxometric results are found to be most consistent with the partitioning of the lipid molecules into affected and unaffected portions, rather than a single averaged phase. Our analysis suggests that up to 25 mol%, each cholesterol molecule orders three DOPC molecules, providing experimental backup to the findings of many molecular dynamics studies. A methodology is established for studying dynamics on multiple timescales in unilamellar membranes of more complex compositions.
Fil: Fraenza, Carla Cecilia. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina
Fil: Meledandri, Carla J.. Universidad de Dublin; Irlanda. University of Otago; Nueva Zelanda
Fil: Anoardo, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Física Enrique Gaviola. Universidad Nacional de Córdoba. Instituto de Física Enrique Gaviola; Argentina. Universidad Nacional de Córdoba. Facultad de Matemática, Astronomía y Física; Argentina
Fil: Brougham, Dermot F.. Universidad de Dublin; Irlanda
description The general applicability of fast field-cycling nuclear magnetic resonance relaxometry in the study of dynamics in lipid bilayers is demonstrated through analysis of binary unilamellar liposomes composed of 1,2-dioleoyl-sn-glycero-3-posphocholine (DOPC) and cholesterol. We extend an evidence-based method to simulating the NMR relaxation response, previously validated for single-component membranes, to evaluate the effect of the sterol molecule on local ordering and dynamics over multiple timescales. The relaxometric results are found to be most consistent with the partitioning of the lipid molecules into affected and unaffected portions, rather than a single averaged phase. Our analysis suggests that up to 25 mol%, each cholesterol molecule orders three DOPC molecules, providing experimental backup to the findings of many molecular dynamics studies. A methodology is established for studying dynamics on multiple timescales in unilamellar membranes of more complex compositions.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/31844
Brougham, Dermot F.; Anoardo, Esteban; Meledandri, Carla J.; Fraenza, Carla Cecilia; The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles; Wiley VCH Verlag; Chemphyschem; 15; 3; 1-2014; 425-435
1439-4235
CONICET Digital
CONICET
url http://hdl.handle.net/11336/31844
identifier_str_mv Brougham, Dermot F.; Anoardo, Esteban; Meledandri, Carla J.; Fraenza, Carla Cecilia; The Effect of Cholesterol on Membrane Dynamics on Different Timescales in Lipid Bilayers from Fast Field-Cycling NMR Relaxometry Studies of Unilamellar Vesicles; Wiley VCH Verlag; Chemphyschem; 15; 3; 1-2014; 425-435
1439-4235
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/cphc.201301051
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/cphc.201301051/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.48226

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