In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment

Autores
Pérez, María Rita; Rossi, Andrea Silvana; Bacchetta, Carla; Elorriaga, Yanina; Carriquiriborde, Pedro; Cazenave, Jimena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Sewage effluents are the most important source of emergent pollutants in the aquatic environment. In thepresent study the toxicological impact of an untreated sewage effluent on the Prochilodus lineatus fish was assessedunder field conditions using a caging experiment. The biomarkers which were measured here involvedoxidative stress markers, hepatic function parameters, neurotoxicity indicators, energy reserves, histologicalalterations and brain cell proliferation. In addition, water quality parameters including the occurrence of somehuman pharmaceuticals were measured. Juveniles of P. lineatus were caged for 15 days at three sites: the effluentsite, and 2 km upstream and downstream from the effluent discharge. Caffeine, atenolol, carbamazepine, enalapriland sildenafil were detected in water river samples. The increased activation of caspase-3 and the decreasedcell proliferation in the diencephalon showed that the brain of fish caged at the effluent was affected.These fish also displayed a rise in hepatic transaminase activity, and oxidative stress in liver and gills which wasevidenced by an increased lipid peroxidation and activation of antioxidant enzymes. At tissue level, increasedglycogen and decreased lipid contents in liver as well as the highest indexes of hepatic lesions were also observedin the fish caged at the effluent. Both biochemical and histopathological findings demonstrated that effects weremore severe on the liver of such fish than on their gills. The in situ exposure method carried out in our studymakes it possible to observe the real effects of the sewage effluent on fish. Furthermore, our results also provide abetter understanding of the harmful effects of wastewater effluents on the aquatic wildlife.
Fil: Pérez, María Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Elorriaga, Yanina. Universidad Nacional de La Plata; Argentina
Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata; Argentina
Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Materia
BIOMARKER
CAGING EXPERIMENT
OXIDATIVE STRESS
CASPASE 3
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/88675
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessmentPérez, María RitaRossi, Andrea SilvanaBacchetta, CarlaElorriaga, YaninaCarriquiriborde, PedroCazenave, JimenaBIOMARKERCAGING EXPERIMENTOXIDATIVE STRESSCASPASE 3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Sewage effluents are the most important source of emergent pollutants in the aquatic environment. In thepresent study the toxicological impact of an untreated sewage effluent on the Prochilodus lineatus fish was assessedunder field conditions using a caging experiment. The biomarkers which were measured here involvedoxidative stress markers, hepatic function parameters, neurotoxicity indicators, energy reserves, histologicalalterations and brain cell proliferation. In addition, water quality parameters including the occurrence of somehuman pharmaceuticals were measured. Juveniles of P. lineatus were caged for 15 days at three sites: the effluentsite, and 2 km upstream and downstream from the effluent discharge. Caffeine, atenolol, carbamazepine, enalapriland sildenafil were detected in water river samples. The increased activation of caspase-3 and the decreasedcell proliferation in the diencephalon showed that the brain of fish caged at the effluent was affected.These fish also displayed a rise in hepatic transaminase activity, and oxidative stress in liver and gills which wasevidenced by an increased lipid peroxidation and activation of antioxidant enzymes. At tissue level, increasedglycogen and decreased lipid contents in liver as well as the highest indexes of hepatic lesions were also observedin the fish caged at the effluent. Both biochemical and histopathological findings demonstrated that effects weremore severe on the liver of such fish than on their gills. The in situ exposure method carried out in our studymakes it possible to observe the real effects of the sewage effluent on fish. Furthermore, our results also provide abetter understanding of the harmful effects of wastewater effluents on the aquatic wildlife.Fil: Pérez, María Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Elorriaga, Yanina. Universidad Nacional de La Plata; ArgentinaFil: Carriquiriborde, Pedro. Universidad Nacional de La Plata; ArgentinaFil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaElsevier Science2018-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/88675Pérez, María Rita; Rossi, Andrea Silvana; Bacchetta, Carla; Elorriaga, Yanina; Carriquiriborde, Pedro; et al.; In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment; Elsevier Science; Ecological Indicators; 84; 1-2018; 345-3531470-160XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S1470160X17305666info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecolind.2017.09.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:01:17Zoai:ri.conicet.gov.ar:11336/88675instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:01:17.976CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
title In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
spellingShingle In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
Pérez, María Rita
BIOMARKER
CAGING EXPERIMENT
OXIDATIVE STRESS
CASPASE 3
title_short In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
title_full In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
title_fullStr In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
title_full_unstemmed In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
title_sort In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment
dc.creator.none.fl_str_mv Pérez, María Rita
Rossi, Andrea Silvana
Bacchetta, Carla
Elorriaga, Yanina
Carriquiriborde, Pedro
Cazenave, Jimena
author Pérez, María Rita
author_facet Pérez, María Rita
Rossi, Andrea Silvana
Bacchetta, Carla
Elorriaga, Yanina
Carriquiriborde, Pedro
Cazenave, Jimena
author_role author
author2 Rossi, Andrea Silvana
Bacchetta, Carla
Elorriaga, Yanina
Carriquiriborde, Pedro
Cazenave, Jimena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BIOMARKER
CAGING EXPERIMENT
OXIDATIVE STRESS
CASPASE 3
topic BIOMARKER
CAGING EXPERIMENT
OXIDATIVE STRESS
CASPASE 3
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Sewage effluents are the most important source of emergent pollutants in the aquatic environment. In thepresent study the toxicological impact of an untreated sewage effluent on the Prochilodus lineatus fish was assessedunder field conditions using a caging experiment. The biomarkers which were measured here involvedoxidative stress markers, hepatic function parameters, neurotoxicity indicators, energy reserves, histologicalalterations and brain cell proliferation. In addition, water quality parameters including the occurrence of somehuman pharmaceuticals were measured. Juveniles of P. lineatus were caged for 15 days at three sites: the effluentsite, and 2 km upstream and downstream from the effluent discharge. Caffeine, atenolol, carbamazepine, enalapriland sildenafil were detected in water river samples. The increased activation of caspase-3 and the decreasedcell proliferation in the diencephalon showed that the brain of fish caged at the effluent was affected.These fish also displayed a rise in hepatic transaminase activity, and oxidative stress in liver and gills which wasevidenced by an increased lipid peroxidation and activation of antioxidant enzymes. At tissue level, increasedglycogen and decreased lipid contents in liver as well as the highest indexes of hepatic lesions were also observedin the fish caged at the effluent. Both biochemical and histopathological findings demonstrated that effects weremore severe on the liver of such fish than on their gills. The in situ exposure method carried out in our studymakes it possible to observe the real effects of the sewage effluent on fish. Furthermore, our results also provide abetter understanding of the harmful effects of wastewater effluents on the aquatic wildlife.
Fil: Pérez, María Rita. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Elorriaga, Yanina. Universidad Nacional de La Plata; Argentina
Fil: Carriquiriborde, Pedro. Universidad Nacional de La Plata; Argentina
Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
description Sewage effluents are the most important source of emergent pollutants in the aquatic environment. In thepresent study the toxicological impact of an untreated sewage effluent on the Prochilodus lineatus fish was assessedunder field conditions using a caging experiment. The biomarkers which were measured here involvedoxidative stress markers, hepatic function parameters, neurotoxicity indicators, energy reserves, histologicalalterations and brain cell proliferation. In addition, water quality parameters including the occurrence of somehuman pharmaceuticals were measured. Juveniles of P. lineatus were caged for 15 days at three sites: the effluentsite, and 2 km upstream and downstream from the effluent discharge. Caffeine, atenolol, carbamazepine, enalapriland sildenafil were detected in water river samples. The increased activation of caspase-3 and the decreasedcell proliferation in the diencephalon showed that the brain of fish caged at the effluent was affected.These fish also displayed a rise in hepatic transaminase activity, and oxidative stress in liver and gills which wasevidenced by an increased lipid peroxidation and activation of antioxidant enzymes. At tissue level, increasedglycogen and decreased lipid contents in liver as well as the highest indexes of hepatic lesions were also observedin the fish caged at the effluent. Both biochemical and histopathological findings demonstrated that effects weremore severe on the liver of such fish than on their gills. The in situ exposure method carried out in our studymakes it possible to observe the real effects of the sewage effluent on fish. Furthermore, our results also provide abetter understanding of the harmful effects of wastewater effluents on the aquatic wildlife.
publishDate 2018
dc.date.none.fl_str_mv 2018-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/88675
Pérez, María Rita; Rossi, Andrea Silvana; Bacchetta, Carla; Elorriaga, Yanina; Carriquiriborde, Pedro; et al.; In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment; Elsevier Science; Ecological Indicators; 84; 1-2018; 345-353
1470-160X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/88675
identifier_str_mv Pérez, María Rita; Rossi, Andrea Silvana; Bacchetta, Carla; Elorriaga, Yanina; Carriquiriborde, Pedro; et al.; In situ evaluation of the toxicological impact of a wastewater effluent on the fish Prochilodus lineatus: biochemical and histological assessment; Elsevier Science; Ecological Indicators; 84; 1-2018; 345-353
1470-160X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S1470160X17305666
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecolind.2017.09.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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