Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish

Autores
Ale, Analía; Rossi, Andrea Silvana; Bacchetta, Carla; Gervasio, Susana Graciela; de la Torre, Fernando Roman; Cazenave, Jimena
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate simultaneously a battery of biomarkers in Prochilodus lineatus fish in order to obtain a holistic overview of silver nanoparticles (AgNP) toxicity. Juveniles were exposed to 2.5 and 25.0 µgAgNP L−1 for 5 and 15 days. We analyzed silver accumulation in tissues (liver, intestine and brain), morphometric and hematological parameters, oxidative stress markers (antioxidant enzymes, lipid peroxidation, and antioxidant capacity against peroxyl radicals-ACAP-), and metabolic responses in plasma (glucose, proteins, triglycerides and cholesterol), liver and muscle (glycogen, proteins, and lipids). Additionally, after 15-day exposure, the number of colony forming units (CFU) of bacteria living at the skin mucus secretion was analyzed to corroborate the AgNP biocide properties. Tissue Ag accumulation was concentration- and time-dependent: liver > intestine > brain. The hepatosomatic index increased at both periods of exposure at 25.0 µgAgNP L−1. Hematological and plasma biochemical parameters increased after 5 days of exposure to the highest concentration. When fish were exposed for 15 days to 25.0 µgAgNP L−1, many changes were observed: glycogen content increased in liver and muscle, muscle protein concentration decreased, all antioxidant enzymes activities were enhanced and ACAP decreased in liver. The CFU decreased in the mucus of fish exposed to both AgNP treatments when compared to the control group. The multivariate analysis revealed a clear difference in physiological profiles of individuals exposed to the different treatments. Our results showed that AgNP in short and subchronic exposures threaten the health of fish.
Fil: Ale, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Gervasio, Susana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: de la Torre, Fernando Roman. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Materia
BACTERIA COLONY FORMING UNITS
BIOMARKERS
NANOTOXICOLOGY
PRINCIPAL COMPONENT ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92187
Acceder
id CONICETDig_dd69ecfa606a3234e471463f1f3f7dab
oai_identifier_str oai:ri.conicet.gov.ar:11336/92187
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fishAle, AnalíaRossi, Andrea SilvanaBacchetta, CarlaGervasio, Susana Gracielade la Torre, Fernando RomanCazenave, JimenaBACTERIA COLONY FORMING UNITSBIOMARKERSNANOTOXICOLOGYPRINCIPAL COMPONENT ANALYSIShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this study was to evaluate simultaneously a battery of biomarkers in Prochilodus lineatus fish in order to obtain a holistic overview of silver nanoparticles (AgNP) toxicity. Juveniles were exposed to 2.5 and 25.0 µgAgNP L−1 for 5 and 15 days. We analyzed silver accumulation in tissues (liver, intestine and brain), morphometric and hematological parameters, oxidative stress markers (antioxidant enzymes, lipid peroxidation, and antioxidant capacity against peroxyl radicals-ACAP-), and metabolic responses in plasma (glucose, proteins, triglycerides and cholesterol), liver and muscle (glycogen, proteins, and lipids). Additionally, after 15-day exposure, the number of colony forming units (CFU) of bacteria living at the skin mucus secretion was analyzed to corroborate the AgNP biocide properties. Tissue Ag accumulation was concentration- and time-dependent: liver > intestine > brain. The hepatosomatic index increased at both periods of exposure at 25.0 µgAgNP L−1. Hematological and plasma biochemical parameters increased after 5 days of exposure to the highest concentration. When fish were exposed for 15 days to 25.0 µgAgNP L−1, many changes were observed: glycogen content increased in liver and muscle, muscle protein concentration decreased, all antioxidant enzymes activities were enhanced and ACAP decreased in liver. The CFU decreased in the mucus of fish exposed to both AgNP treatments when compared to the control group. The multivariate analysis revealed a clear difference in physiological profiles of individuals exposed to the different treatments. Our results showed that AgNP in short and subchronic exposures threaten the health of fish.Fil: Ale, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: Gervasio, Susana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaFil: de la Torre, Fernando Roman. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; ArgentinaFil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; ArgentinaElsevier Science2018-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92187Ale, Analía; Rossi, Andrea Silvana; Bacchetta, Carla; Gervasio, Susana Graciela; de la Torre, Fernando Roman; et al.; Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish; Elsevier Science; Ecological Indicators; 93; 10-2018; 1190-11981470-160XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecolind.2018.06.023info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1470160X18304631info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:28:48Zoai:ri.conicet.gov.ar:11336/92187instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:28:48.895CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish
title Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish
spellingShingle Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish
Ale, Analía
BACTERIA COLONY FORMING UNITS
BIOMARKERS
NANOTOXICOLOGY
PRINCIPAL COMPONENT ANALYSIS
title_short Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish
title_full Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish
title_fullStr Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish
title_full_unstemmed Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish
title_sort Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish
dc.creator.none.fl_str_mv Ale, Analía
Rossi, Andrea Silvana
Bacchetta, Carla
Gervasio, Susana Graciela
de la Torre, Fernando Roman
Cazenave, Jimena
author Ale, Analía
author_facet Ale, Analía
Rossi, Andrea Silvana
Bacchetta, Carla
Gervasio, Susana Graciela
de la Torre, Fernando Roman
Cazenave, Jimena
author_role author
author2 Rossi, Andrea Silvana
Bacchetta, Carla
Gervasio, Susana Graciela
de la Torre, Fernando Roman
Cazenave, Jimena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv BACTERIA COLONY FORMING UNITS
BIOMARKERS
NANOTOXICOLOGY
PRINCIPAL COMPONENT ANALYSIS
topic BACTERIA COLONY FORMING UNITS
BIOMARKERS
NANOTOXICOLOGY
PRINCIPAL COMPONENT ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to evaluate simultaneously a battery of biomarkers in Prochilodus lineatus fish in order to obtain a holistic overview of silver nanoparticles (AgNP) toxicity. Juveniles were exposed to 2.5 and 25.0 µgAgNP L−1 for 5 and 15 days. We analyzed silver accumulation in tissues (liver, intestine and brain), morphometric and hematological parameters, oxidative stress markers (antioxidant enzymes, lipid peroxidation, and antioxidant capacity against peroxyl radicals-ACAP-), and metabolic responses in plasma (glucose, proteins, triglycerides and cholesterol), liver and muscle (glycogen, proteins, and lipids). Additionally, after 15-day exposure, the number of colony forming units (CFU) of bacteria living at the skin mucus secretion was analyzed to corroborate the AgNP biocide properties. Tissue Ag accumulation was concentration- and time-dependent: liver > intestine > brain. The hepatosomatic index increased at both periods of exposure at 25.0 µgAgNP L−1. Hematological and plasma biochemical parameters increased after 5 days of exposure to the highest concentration. When fish were exposed for 15 days to 25.0 µgAgNP L−1, many changes were observed: glycogen content increased in liver and muscle, muscle protein concentration decreased, all antioxidant enzymes activities were enhanced and ACAP decreased in liver. The CFU decreased in the mucus of fish exposed to both AgNP treatments when compared to the control group. The multivariate analysis revealed a clear difference in physiological profiles of individuals exposed to the different treatments. Our results showed that AgNP in short and subchronic exposures threaten the health of fish.
Fil: Ale, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Rossi, Andrea Silvana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: Gervasio, Susana Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
Fil: de la Torre, Fernando Roman. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ecología y Desarrollo Sustentable; Argentina
Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina
description The aim of this study was to evaluate simultaneously a battery of biomarkers in Prochilodus lineatus fish in order to obtain a holistic overview of silver nanoparticles (AgNP) toxicity. Juveniles were exposed to 2.5 and 25.0 µgAgNP L−1 for 5 and 15 days. We analyzed silver accumulation in tissues (liver, intestine and brain), morphometric and hematological parameters, oxidative stress markers (antioxidant enzymes, lipid peroxidation, and antioxidant capacity against peroxyl radicals-ACAP-), and metabolic responses in plasma (glucose, proteins, triglycerides and cholesterol), liver and muscle (glycogen, proteins, and lipids). Additionally, after 15-day exposure, the number of colony forming units (CFU) of bacteria living at the skin mucus secretion was analyzed to corroborate the AgNP biocide properties. Tissue Ag accumulation was concentration- and time-dependent: liver > intestine > brain. The hepatosomatic index increased at both periods of exposure at 25.0 µgAgNP L−1. Hematological and plasma biochemical parameters increased after 5 days of exposure to the highest concentration. When fish were exposed for 15 days to 25.0 µgAgNP L−1, many changes were observed: glycogen content increased in liver and muscle, muscle protein concentration decreased, all antioxidant enzymes activities were enhanced and ACAP decreased in liver. The CFU decreased in the mucus of fish exposed to both AgNP treatments when compared to the control group. The multivariate analysis revealed a clear difference in physiological profiles of individuals exposed to the different treatments. Our results showed that AgNP in short and subchronic exposures threaten the health of fish.
publishDate 2018
dc.date.none.fl_str_mv 2018-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92187
Ale, Analía; Rossi, Andrea Silvana; Bacchetta, Carla; Gervasio, Susana Graciela; de la Torre, Fernando Roman; et al.; Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish; Elsevier Science; Ecological Indicators; 93; 10-2018; 1190-1198
1470-160X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92187
identifier_str_mv Ale, Analía; Rossi, Andrea Silvana; Bacchetta, Carla; Gervasio, Susana Graciela; de la Torre, Fernando Roman; et al.; Integrative assessment of silver nanoparticles toxicity in Prochilodus lineatus fish; Elsevier Science; Ecological Indicators; 93; 10-2018; 1190-1198
1470-160X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ecolind.2018.06.023
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1470160X18304631
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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