UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role

Autores
Flores, María Regina; Farias, Maria Eugenia
Año de publicación
2011
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
In order to test UV-B radiation resistance of bacterioplankton communities from High Altitude Andean Wetlands, water samples were exposed 24 h to UV-B radiation (DOSIS: 166 kJ/m2). A red bacterial strain related to Serratia sp. Vil 11, was able to resist more than 6 h under UV-B treatment. In this work, we analyze the photoprotective pigment role in Serratia sp. Vil 11 employing a wild type strain (red) and a non-pigmented mutant. In addition, we also performed the identification of the pigment content by HPLC and it cellular localization by confocal microscopy. In the absence of pigment production, Serratia sp. Vil 11 was significantly more susceptible to UV-B stress. Pigmented cells survived high UV-B exposure (DOSIS: 7 kJ/m2), around 10 fold compared to the non-pigmented mutant cells. The HPLC analyses showed a sole peak corresponding to prodigiosine pigment and the confocal microscopy analysis showed an extracellular location of the pigment distribution suggesting that it would be play an important role in primary protection against UV-B radiation.
Fil: Flores, María Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VII Congreso Argentino de Microbiología General
San Miguel de Tucumán
Argentina
Sociedad Argentina de Microbiología General
Materia
UV-B RADIATION
HIGH ALTITUDE WETLANDS
BACTERIA
PIGMENT
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/192045
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/192045
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment roleFlores, María ReginaFarias, Maria EugeniaUV-B RADIATIONHIGH ALTITUDE WETLANDSBACTERIAPIGMENThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In order to test UV-B radiation resistance of bacterioplankton communities from High Altitude Andean Wetlands, water samples were exposed 24 h to UV-B radiation (DOSIS: 166 kJ/m2). A red bacterial strain related to Serratia sp. Vil 11, was able to resist more than 6 h under UV-B treatment. In this work, we analyze the photoprotective pigment role in Serratia sp. Vil 11 employing a wild type strain (red) and a non-pigmented mutant. In addition, we also performed the identification of the pigment content by HPLC and it cellular localization by confocal microscopy. In the absence of pigment production, Serratia sp. Vil 11 was significantly more susceptible to UV-B stress. Pigmented cells survived high UV-B exposure (DOSIS: 7 kJ/m2), around 10 fold compared to the non-pigmented mutant cells. The HPLC analyses showed a sole peak corresponding to prodigiosine pigment and the confocal microscopy analysis showed an extracellular location of the pigment distribution suggesting that it would be play an important role in primary protection against UV-B radiation.Fil: Flores, María Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaVII Congreso Argentino de Microbiología GeneralSan Miguel de TucumánArgentinaSociedad Argentina de Microbiología GeneralSociedad Argentina de Microbiología General2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/192045UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role; VII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2011; 1-2CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2011.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:12:57Zoai:ri.conicet.gov.ar:11336/192045instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:12:57.788CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role
title UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role
spellingShingle UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role
Flores, María Regina
UV-B RADIATION
HIGH ALTITUDE WETLANDS
BACTERIA
PIGMENT
title_short UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role
title_full UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role
title_fullStr UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role
title_full_unstemmed UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role
title_sort UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role
dc.creator.none.fl_str_mv Flores, María Regina
Farias, Maria Eugenia
author Flores, María Regina
author_facet Flores, María Regina
Farias, Maria Eugenia
author_role author
author2 Farias, Maria Eugenia
author2_role author
dc.subject.none.fl_str_mv UV-B RADIATION
HIGH ALTITUDE WETLANDS
BACTERIA
PIGMENT
topic UV-B RADIATION
HIGH ALTITUDE WETLANDS
BACTERIA
PIGMENT
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In order to test UV-B radiation resistance of bacterioplankton communities from High Altitude Andean Wetlands, water samples were exposed 24 h to UV-B radiation (DOSIS: 166 kJ/m2). A red bacterial strain related to Serratia sp. Vil 11, was able to resist more than 6 h under UV-B treatment. In this work, we analyze the photoprotective pigment role in Serratia sp. Vil 11 employing a wild type strain (red) and a non-pigmented mutant. In addition, we also performed the identification of the pigment content by HPLC and it cellular localization by confocal microscopy. In the absence of pigment production, Serratia sp. Vil 11 was significantly more susceptible to UV-B stress. Pigmented cells survived high UV-B exposure (DOSIS: 7 kJ/m2), around 10 fold compared to the non-pigmented mutant cells. The HPLC analyses showed a sole peak corresponding to prodigiosine pigment and the confocal microscopy analysis showed an extracellular location of the pigment distribution suggesting that it would be play an important role in primary protection against UV-B radiation.
Fil: Flores, María Regina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
VII Congreso Argentino de Microbiología General
San Miguel de Tucumán
Argentina
Sociedad Argentina de Microbiología General
description In order to test UV-B radiation resistance of bacterioplankton communities from High Altitude Andean Wetlands, water samples were exposed 24 h to UV-B radiation (DOSIS: 166 kJ/m2). A red bacterial strain related to Serratia sp. Vil 11, was able to resist more than 6 h under UV-B treatment. In this work, we analyze the photoprotective pigment role in Serratia sp. Vil 11 employing a wild type strain (red) and a non-pigmented mutant. In addition, we also performed the identification of the pigment content by HPLC and it cellular localization by confocal microscopy. In the absence of pigment production, Serratia sp. Vil 11 was significantly more susceptible to UV-B stress. Pigmented cells survived high UV-B exposure (DOSIS: 7 kJ/m2), around 10 fold compared to the non-pigmented mutant cells. The HPLC analyses showed a sole peak corresponding to prodigiosine pigment and the confocal microscopy analysis showed an extracellular location of the pigment distribution suggesting that it would be play an important role in primary protection against UV-B radiation.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/192045
UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role; VII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2011; 1-2
CONICET Digital
CONICET
url http://hdl.handle.net/11336/192045
identifier_str_mv UV-B resistance in a bacterial strain isolated from High Altitude Andean Wetlands: the pigment role; VII Congreso Argentino de Microbiología General; San Miguel de Tucumán; Argentina; 2011; 1-2
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://samige.org.ar/wp-content/uploads/2022/10/Libro-SAMIGE-2011.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
publisher.none.fl_str_mv Sociedad Argentina de Microbiología General
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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