Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile

Autores
Escudero, Lorena; Chong, Guillermo; Demergasso, Cecilia; Farias, Maria Eugenia; Cabrol, Nathalie A.; Grin, Edmond; Minkley Jr., Edwin; Yu, Youngeob
Año de publicación
2007
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The High-Lakes Project is funded by the NAI and explores the highest perennial volcanic lakes on Earth in the Bolivian and Chilean Andes, including several lakes ∼6,000 m elevation. These lakes represent an opportunity to study the evolution of microbial organisms in relatively shallow waters not providing substantial protection against UV radiation. Aguas Calientes (5,870 m) was investigated (November 2006) and samples of water and sediment collected at 1, 3, 5, and 10 cm depth. An Eldonet UV dosimeter positioned on the shore records UV radiation and temperature, and is logging data year round. A UV SolarLight sensor allowed acquisition of point measurements in all channels at the time of the sampling. UVA, UVB, and PAR peaks between 11:00 am and 1:00 pm reached 7.7 mW/cm2, 48.5 μW/cm2, and 511 W/m 2, respectively. The chemical composition of the water sample was analyzed. DNA was extracted and DGGE analyses with bacterial and archaeal 16S fragments were performed to describe microbial diversity. Antibiotic resistances were established previously in similar environments in Argentine Andean wetlands. In order to determine these resistances in our samples, they were inoculated onto LB and R2A media and onto R2A medium containing either chloramphenicol, ampicillin or tetracycline. Bacterial was higher than archeal cell number determined by RT-PCR in all the samples, reaching maximum total values of 5×105 cell mL-1. DGGE results from these samples and Licancabur summit lake (5,916 m) samples were also compared. Eight antibiotic-resistant Gram negative strains have been isolated with distinct resistance patterns.
Fil: Escudero, Lorena. Centro de Investigación Científica y Tecnológica para la Minería; Argentina
Fil: Chong, Guillermo. Centro de Investigación Científica y Tecnológica para la Minería; Argentina
Fil: Demergasso, Cecilia. Universidad Católica de Chile; Chile
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cabrol, Nathalie A.. Nasa Ames Research Center; Estados Unidos
Fil: Grin, Edmond. Nasa Ames Research Center; Estados Unidos
Fil: Minkley Jr., Edwin. University of Carnegie Mellon; Estados Unidos
Fil: Yu, Youngeob. University of Carnegie Mellon; Estados Unidos
Materia
ANTIBIOTIC RESISTANCE
HIGH ALTITUDE LAKES
MICROBIAL DIVERSITY
UV RESISTANCE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/92123
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/92123
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, ChileEscudero, LorenaChong, GuillermoDemergasso, CeciliaFarias, Maria EugeniaCabrol, Nathalie A.Grin, EdmondMinkley Jr., EdwinYu, YoungeobANTIBIOTIC RESISTANCEHIGH ALTITUDE LAKESMICROBIAL DIVERSITYUV RESISTANCEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The High-Lakes Project is funded by the NAI and explores the highest perennial volcanic lakes on Earth in the Bolivian and Chilean Andes, including several lakes ∼6,000 m elevation. These lakes represent an opportunity to study the evolution of microbial organisms in relatively shallow waters not providing substantial protection against UV radiation. Aguas Calientes (5,870 m) was investigated (November 2006) and samples of water and sediment collected at 1, 3, 5, and 10 cm depth. An Eldonet UV dosimeter positioned on the shore records UV radiation and temperature, and is logging data year round. A UV SolarLight sensor allowed acquisition of point measurements in all channels at the time of the sampling. UVA, UVB, and PAR peaks between 11:00 am and 1:00 pm reached 7.7 mW/cm2, 48.5 μW/cm2, and 511 W/m 2, respectively. The chemical composition of the water sample was analyzed. DNA was extracted and DGGE analyses with bacterial and archaeal 16S fragments were performed to describe microbial diversity. Antibiotic resistances were established previously in similar environments in Argentine Andean wetlands. In order to determine these resistances in our samples, they were inoculated onto LB and R2A media and onto R2A medium containing either chloramphenicol, ampicillin or tetracycline. Bacterial was higher than archeal cell number determined by RT-PCR in all the samples, reaching maximum total values of 5×105 cell mL-1. DGGE results from these samples and Licancabur summit lake (5,916 m) samples were also compared. Eight antibiotic-resistant Gram negative strains have been isolated with distinct resistance patterns.Fil: Escudero, Lorena. Centro de Investigación Científica y Tecnológica para la Minería; ArgentinaFil: Chong, Guillermo. Centro de Investigación Científica y Tecnológica para la Minería; ArgentinaFil: Demergasso, Cecilia. Universidad Católica de Chile; ChileFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Cabrol, Nathalie A.. Nasa Ames Research Center; Estados UnidosFil: Grin, Edmond. Nasa Ames Research Center; Estados UnidosFil: Minkley Jr., Edwin. University of Carnegie Mellon; Estados UnidosFil: Yu, Youngeob. University of Carnegie Mellon; Estados UnidosSPIE2007-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/92123Escudero, Lorena; Chong, Guillermo; Demergasso, Cecilia; Farias, Maria Eugenia; Cabrol, Nathalie A.; et al.; Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile; SPIE; Spie; 6694; 12-20070277-786XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://spie.org/x648.xml?product_id=736970&origin_id=x1636&Search_Results_URL=http://spie.org/x1636.xml&Search_Origin=ResearchSearch&category=ResearchPapers&isResearch=true&UseJavascript=1&Please_Wait_URL=http://spie.org/x18503.xml&title=Investigating%20Minfo:eu-repo/semantics/altIdentifier/doi/10.1117/12.736970info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:55:36Zoai:ri.conicet.gov.ar:11336/92123instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:55:37.047CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile
title Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile
spellingShingle Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile
Escudero, Lorena
ANTIBIOTIC RESISTANCE
HIGH ALTITUDE LAKES
MICROBIAL DIVERSITY
UV RESISTANCE
title_short Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile
title_full Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile
title_fullStr Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile
title_full_unstemmed Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile
title_sort Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile
dc.creator.none.fl_str_mv Escudero, Lorena
Chong, Guillermo
Demergasso, Cecilia
Farias, Maria Eugenia
Cabrol, Nathalie A.
Grin, Edmond
Minkley Jr., Edwin
Yu, Youngeob
author Escudero, Lorena
author_facet Escudero, Lorena
Chong, Guillermo
Demergasso, Cecilia
Farias, Maria Eugenia
Cabrol, Nathalie A.
Grin, Edmond
Minkley Jr., Edwin
Yu, Youngeob
author_role author
author2 Chong, Guillermo
Demergasso, Cecilia
Farias, Maria Eugenia
Cabrol, Nathalie A.
Grin, Edmond
Minkley Jr., Edwin
Yu, Youngeob
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBIOTIC RESISTANCE
HIGH ALTITUDE LAKES
MICROBIAL DIVERSITY
UV RESISTANCE
topic ANTIBIOTIC RESISTANCE
HIGH ALTITUDE LAKES
MICROBIAL DIVERSITY
UV RESISTANCE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The High-Lakes Project is funded by the NAI and explores the highest perennial volcanic lakes on Earth in the Bolivian and Chilean Andes, including several lakes ∼6,000 m elevation. These lakes represent an opportunity to study the evolution of microbial organisms in relatively shallow waters not providing substantial protection against UV radiation. Aguas Calientes (5,870 m) was investigated (November 2006) and samples of water and sediment collected at 1, 3, 5, and 10 cm depth. An Eldonet UV dosimeter positioned on the shore records UV radiation and temperature, and is logging data year round. A UV SolarLight sensor allowed acquisition of point measurements in all channels at the time of the sampling. UVA, UVB, and PAR peaks between 11:00 am and 1:00 pm reached 7.7 mW/cm2, 48.5 μW/cm2, and 511 W/m 2, respectively. The chemical composition of the water sample was analyzed. DNA was extracted and DGGE analyses with bacterial and archaeal 16S fragments were performed to describe microbial diversity. Antibiotic resistances were established previously in similar environments in Argentine Andean wetlands. In order to determine these resistances in our samples, they were inoculated onto LB and R2A media and onto R2A medium containing either chloramphenicol, ampicillin or tetracycline. Bacterial was higher than archeal cell number determined by RT-PCR in all the samples, reaching maximum total values of 5×105 cell mL-1. DGGE results from these samples and Licancabur summit lake (5,916 m) samples were also compared. Eight antibiotic-resistant Gram negative strains have been isolated with distinct resistance patterns.
Fil: Escudero, Lorena. Centro de Investigación Científica y Tecnológica para la Minería; Argentina
Fil: Chong, Guillermo. Centro de Investigación Científica y Tecnológica para la Minería; Argentina
Fil: Demergasso, Cecilia. Universidad Católica de Chile; Chile
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cabrol, Nathalie A.. Nasa Ames Research Center; Estados Unidos
Fil: Grin, Edmond. Nasa Ames Research Center; Estados Unidos
Fil: Minkley Jr., Edwin. University of Carnegie Mellon; Estados Unidos
Fil: Yu, Youngeob. University of Carnegie Mellon; Estados Unidos
description The High-Lakes Project is funded by the NAI and explores the highest perennial volcanic lakes on Earth in the Bolivian and Chilean Andes, including several lakes ∼6,000 m elevation. These lakes represent an opportunity to study the evolution of microbial organisms in relatively shallow waters not providing substantial protection against UV radiation. Aguas Calientes (5,870 m) was investigated (November 2006) and samples of water and sediment collected at 1, 3, 5, and 10 cm depth. An Eldonet UV dosimeter positioned on the shore records UV radiation and temperature, and is logging data year round. A UV SolarLight sensor allowed acquisition of point measurements in all channels at the time of the sampling. UVA, UVB, and PAR peaks between 11:00 am and 1:00 pm reached 7.7 mW/cm2, 48.5 μW/cm2, and 511 W/m 2, respectively. The chemical composition of the water sample was analyzed. DNA was extracted and DGGE analyses with bacterial and archaeal 16S fragments were performed to describe microbial diversity. Antibiotic resistances were established previously in similar environments in Argentine Andean wetlands. In order to determine these resistances in our samples, they were inoculated onto LB and R2A media and onto R2A medium containing either chloramphenicol, ampicillin or tetracycline. Bacterial was higher than archeal cell number determined by RT-PCR in all the samples, reaching maximum total values of 5×105 cell mL-1. DGGE results from these samples and Licancabur summit lake (5,916 m) samples were also compared. Eight antibiotic-resistant Gram negative strains have been isolated with distinct resistance patterns.
publishDate 2007
dc.date.none.fl_str_mv 2007-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/92123
Escudero, Lorena; Chong, Guillermo; Demergasso, Cecilia; Farias, Maria Eugenia; Cabrol, Nathalie A.; et al.; Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile; SPIE; Spie; 6694; 12-2007
0277-786X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/92123
identifier_str_mv Escudero, Lorena; Chong, Guillermo; Demergasso, Cecilia; Farias, Maria Eugenia; Cabrol, Nathalie A.; et al.; Investigating microbial diversity and UV radiation impact at the high-altitude lake Aguas Calientes, Chile; SPIE; Spie; 6694; 12-2007
0277-786X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://spie.org/x648.xml?product_id=736970&origin_id=x1636&Search_Results_URL=http://spie.org/x1636.xml&Search_Origin=ResearchSearch&category=ResearchPapers&isResearch=true&UseJavascript=1&Please_Wait_URL=http://spie.org/x18503.xml&title=Investigating%20M
info:eu-repo/semantics/altIdentifier/doi/10.1117/12.736970
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv SPIE
publisher.none.fl_str_mv SPIE
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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