Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula

Autores
Carballo, José; Zappacosta, Diego Carlos; Marconi, Gianpiero; Gallardo, Jimena Alicia; Di Marsico, Marco; Gallo, Cristian Andrés; Cáccamo, Mario José; Albertini, Emidio; Echenique, Carmen Viviana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
DNA methylation is an epigenetic mechanism by which a methyl group is added to a cytosine or an adenine. When located in a gene/regulatory sequence it may repress or de-repress genes, depending on the context and species. Eragrostis curvula is an apomictic grass in which facultative genotypes increases the frequency of sexual pistils triggered by epigenetic mechanisms. The aim of the present study was to look for correlations between the reproductive mode and specific methylated genes or genomic regions. To do so, plants with contrasting reproductive modes were investigated through MCSeEd (Methylation Context Sensitive Enzyme ddRad) showing higher levels of DNA methylation in apomictic genotypes. Moreover, an increased proportion of differentially methylated positions over the regulatory regions were observed, suggesting its possible role in regulation of gene expression. Interestingly, the methylation pathway was also found to be self-regulated since two of the main genes (ROS1 and ROS4), involved in de-methylation, were found differentially methylated between genotypes with different reproductive behavior. Moreover, this work allowed us to detect several genes regulated by methylation that were previously found as differentially expressed in the comparisons between apomictic and sexual genotypes, linking DNA methylation to differences in reproductive mode.
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Marconi, Gianpiero. Università di Perugia; Italia
Fil: Gallardo, Jimena Alicia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Di Marsico, Marco. Università di Perugia; Italia
Fil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Cáccamo, Mario José. National Institute Of Agricultural Botany.; Reino Unido
Fil: Albertini, Emidio. Università di Perugia; Italia
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
APOMIXIS
DIPLOSPORY
DNA METHYLATION
EPIGENETICS
ERAGROSTIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/167182
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvulaCarballo, JoséZappacosta, Diego CarlosMarconi, GianpieroGallardo, Jimena AliciaDi Marsico, MarcoGallo, Cristian AndrésCáccamo, Mario JoséAlbertini, EmidioEchenique, Carmen VivianaAPOMIXISDIPLOSPORYDNA METHYLATIONEPIGENETICSERAGROSTIShttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4DNA methylation is an epigenetic mechanism by which a methyl group is added to a cytosine or an adenine. When located in a gene/regulatory sequence it may repress or de-repress genes, depending on the context and species. Eragrostis curvula is an apomictic grass in which facultative genotypes increases the frequency of sexual pistils triggered by epigenetic mechanisms. The aim of the present study was to look for correlations between the reproductive mode and specific methylated genes or genomic regions. To do so, plants with contrasting reproductive modes were investigated through MCSeEd (Methylation Context Sensitive Enzyme ddRad) showing higher levels of DNA methylation in apomictic genotypes. Moreover, an increased proportion of differentially methylated positions over the regulatory regions were observed, suggesting its possible role in regulation of gene expression. Interestingly, the methylation pathway was also found to be self-regulated since two of the main genes (ROS1 and ROS4), involved in de-methylation, were found differentially methylated between genotypes with different reproductive behavior. Moreover, this work allowed us to detect several genes regulated by methylation that were previously found as differentially expressed in the comparisons between apomictic and sexual genotypes, linking DNA methylation to differences in reproductive mode.Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Marconi, Gianpiero. Università di Perugia; ItaliaFil: Gallardo, Jimena Alicia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Di Marsico, Marco. Università di Perugia; ItaliaFil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Cáccamo, Mario José. National Institute Of Agricultural Botany.; Reino UnidoFil: Albertini, Emidio. Università di Perugia; ItaliaFil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaMultidisciplinary Digital Publishing Institute2021-05-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/167182Carballo, José; Zappacosta, Diego Carlos; Marconi, Gianpiero; Gallardo, Jimena Alicia; Di Marsico, Marco; et al.; Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula; Multidisciplinary Digital Publishing Institute; Plants; 10; 5; 10-5-2021; 1-182223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/5/946info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10050946info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:03Zoai:ri.conicet.gov.ar:11336/167182instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:04.272CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula
title Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula
spellingShingle Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula
Carballo, José
APOMIXIS
DIPLOSPORY
DNA METHYLATION
EPIGENETICS
ERAGROSTIS
title_short Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula
title_full Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula
title_fullStr Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula
title_full_unstemmed Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula
title_sort Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula
dc.creator.none.fl_str_mv Carballo, José
Zappacosta, Diego Carlos
Marconi, Gianpiero
Gallardo, Jimena Alicia
Di Marsico, Marco
Gallo, Cristian Andrés
Cáccamo, Mario José
Albertini, Emidio
Echenique, Carmen Viviana
author Carballo, José
author_facet Carballo, José
Zappacosta, Diego Carlos
Marconi, Gianpiero
Gallardo, Jimena Alicia
Di Marsico, Marco
Gallo, Cristian Andrés
Cáccamo, Mario José
Albertini, Emidio
Echenique, Carmen Viviana
author_role author
author2 Zappacosta, Diego Carlos
Marconi, Gianpiero
Gallardo, Jimena Alicia
Di Marsico, Marco
Gallo, Cristian Andrés
Cáccamo, Mario José
Albertini, Emidio
Echenique, Carmen Viviana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOMIXIS
DIPLOSPORY
DNA METHYLATION
EPIGENETICS
ERAGROSTIS
topic APOMIXIS
DIPLOSPORY
DNA METHYLATION
EPIGENETICS
ERAGROSTIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv DNA methylation is an epigenetic mechanism by which a methyl group is added to a cytosine or an adenine. When located in a gene/regulatory sequence it may repress or de-repress genes, depending on the context and species. Eragrostis curvula is an apomictic grass in which facultative genotypes increases the frequency of sexual pistils triggered by epigenetic mechanisms. The aim of the present study was to look for correlations between the reproductive mode and specific methylated genes or genomic regions. To do so, plants with contrasting reproductive modes were investigated through MCSeEd (Methylation Context Sensitive Enzyme ddRad) showing higher levels of DNA methylation in apomictic genotypes. Moreover, an increased proportion of differentially methylated positions over the regulatory regions were observed, suggesting its possible role in regulation of gene expression. Interestingly, the methylation pathway was also found to be self-regulated since two of the main genes (ROS1 and ROS4), involved in de-methylation, were found differentially methylated between genotypes with different reproductive behavior. Moreover, this work allowed us to detect several genes regulated by methylation that were previously found as differentially expressed in the comparisons between apomictic and sexual genotypes, linking DNA methylation to differences in reproductive mode.
Fil: Carballo, José. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Zappacosta, Diego Carlos. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Marconi, Gianpiero. Università di Perugia; Italia
Fil: Gallardo, Jimena Alicia. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Di Marsico, Marco. Università di Perugia; Italia
Fil: Gallo, Cristian Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Cáccamo, Mario José. National Institute Of Agricultural Botany.; Reino Unido
Fil: Albertini, Emidio. Università di Perugia; Italia
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description DNA methylation is an epigenetic mechanism by which a methyl group is added to a cytosine or an adenine. When located in a gene/regulatory sequence it may repress or de-repress genes, depending on the context and species. Eragrostis curvula is an apomictic grass in which facultative genotypes increases the frequency of sexual pistils triggered by epigenetic mechanisms. The aim of the present study was to look for correlations between the reproductive mode and specific methylated genes or genomic regions. To do so, plants with contrasting reproductive modes were investigated through MCSeEd (Methylation Context Sensitive Enzyme ddRad) showing higher levels of DNA methylation in apomictic genotypes. Moreover, an increased proportion of differentially methylated positions over the regulatory regions were observed, suggesting its possible role in regulation of gene expression. Interestingly, the methylation pathway was also found to be self-regulated since two of the main genes (ROS1 and ROS4), involved in de-methylation, were found differentially methylated between genotypes with different reproductive behavior. Moreover, this work allowed us to detect several genes regulated by methylation that were previously found as differentially expressed in the comparisons between apomictic and sexual genotypes, linking DNA methylation to differences in reproductive mode.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/167182
Carballo, José; Zappacosta, Diego Carlos; Marconi, Gianpiero; Gallardo, Jimena Alicia; Di Marsico, Marco; et al.; Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula; Multidisciplinary Digital Publishing Institute; Plants; 10; 5; 10-5-2021; 1-18
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/167182
identifier_str_mv Carballo, José; Zappacosta, Diego Carlos; Marconi, Gianpiero; Gallardo, Jimena Alicia; Di Marsico, Marco; et al.; Differential methylation patterns in apomictic vs. Sexual genotypes of the diplosporous grass Eragrostis curvula; Multidisciplinary Digital Publishing Institute; Plants; 10; 5; 10-5-2021; 1-18
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/5/946
info:eu-repo/semantics/altIdentifier/doi/10.3390/plants10050946
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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