Apomixis expression in Eragrostis curvula under stress conditions

Autores
Zappacosta, Diego Carlos; Ochogavía, Ana Claudia; Romero, José Rodolfo; Rodrigo, Juan Manuel; Meier, Mauro Sebastián; Polci, Pablo Aldo; Garbus, Ingrid; Pessino, Silvina Claudia; Echenique, Carmen Viviana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Over the past few years our research group has been analyzing the molecular mechanisms involved in the reproductive control of weeping lovegrass (Eragrostis curvula [Schrad.] Nees), an apomictic perennial grass. This work was aimed at analyzing apomixis expressivity in Eragrostis curvula plants subjected to three stress conditions: 1) tissue culture followed by a colchicine-induced ploidy change; 2) tissue culture and 3) water deficit. In the first system, a colchicine-induced polyploid plant (C) changed the expressivity of apomixis in a four year period, being highly sexual shortly after polyploidization and highly apomictic four years later. During this interval, genetic and epigenetic changes were also observed. While genetic variation led to diversification with respect to an obligate apomictic control, epigenetic variation resulted in increased similarity. Polymorphic sequences were mainly similar to transposable elements and/or protein-coding-like sequences. In the second system, explants originated from near obligate apomictic plants were subjected to tissue culture. The expressivity of apomixis of a regenerated plant was analyzed immediately after treatment and nine years later. In this period, the levels of sexuality shifted from 45 to 0%. In the third model, near-obligate apomictic plants subjected to water stress showed an increase in expression of sexuality from 2 to 20%. As a whole, our results suggested that stress can temporarily activate sexuality in apomictic genotypes. At least in one system, this change was correlated with genetic and epigenetic modifications involving retroelements and/or protein-coding-like sequences.
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Ochogavía, Ana Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, José Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Meier, Mauro Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
International Plant and Animal Genome XXII Conference
San Diego
Estados Unidos
Scherago International
Materia
APOMIXIS
ERAGROSTIS CURVULA
STRESS CONDITIONS
EPIGENETICS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/232082
Acceder
id CONICETDig_3449c5febc729cccb02abbbab2eb271f
oai_identifier_str oai:ri.conicet.gov.ar:11336/232082
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Apomixis expression in Eragrostis curvula under stress conditionsZappacosta, Diego CarlosOchogavía, Ana ClaudiaRomero, José RodolfoRodrigo, Juan ManuelMeier, Mauro SebastiánPolci, Pablo AldoGarbus, IngridPessino, Silvina ClaudiaEchenique, Carmen VivianaAPOMIXISERAGROSTIS CURVULASTRESS CONDITIONSEPIGENETICShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Over the past few years our research group has been analyzing the molecular mechanisms involved in the reproductive control of weeping lovegrass (Eragrostis curvula [Schrad.] Nees), an apomictic perennial grass. This work was aimed at analyzing apomixis expressivity in Eragrostis curvula plants subjected to three stress conditions: 1) tissue culture followed by a colchicine-induced ploidy change; 2) tissue culture and 3) water deficit. In the first system, a colchicine-induced polyploid plant (C) changed the expressivity of apomixis in a four year period, being highly sexual shortly after polyploidization and highly apomictic four years later. During this interval, genetic and epigenetic changes were also observed. While genetic variation led to diversification with respect to an obligate apomictic control, epigenetic variation resulted in increased similarity. Polymorphic sequences were mainly similar to transposable elements and/or protein-coding-like sequences. In the second system, explants originated from near obligate apomictic plants were subjected to tissue culture. The expressivity of apomixis of a regenerated plant was analyzed immediately after treatment and nine years later. In this period, the levels of sexuality shifted from 45 to 0%. In the third model, near-obligate apomictic plants subjected to water stress showed an increase in expression of sexuality from 2 to 20%. As a whole, our results suggested that stress can temporarily activate sexuality in apomictic genotypes. At least in one system, this change was correlated with genetic and epigenetic modifications involving retroelements and/or protein-coding-like sequences.Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Ochogavía, Ana Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romero, José Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Meier, Mauro Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaInternational Plant and Animal Genome XXII ConferenceSan DiegoEstados UnidosScherago InternationalScherago International2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/232082Apomixis expression in Eragrostis curvula under stress conditions; International Plant and Animal Genome XXII Conference; San Diego; Estados Unidos; 2014; 1-1CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pag.confex.com/pag/xxii/webprogram/Paper10223.htmlInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:49:34Zoai:ri.conicet.gov.ar:11336/232082instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:49:35.117CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Apomixis expression in Eragrostis curvula under stress conditions
title Apomixis expression in Eragrostis curvula under stress conditions
spellingShingle Apomixis expression in Eragrostis curvula under stress conditions
Zappacosta, Diego Carlos
APOMIXIS
ERAGROSTIS CURVULA
STRESS CONDITIONS
EPIGENETICS
title_short Apomixis expression in Eragrostis curvula under stress conditions
title_full Apomixis expression in Eragrostis curvula under stress conditions
title_fullStr Apomixis expression in Eragrostis curvula under stress conditions
title_full_unstemmed Apomixis expression in Eragrostis curvula under stress conditions
title_sort Apomixis expression in Eragrostis curvula under stress conditions
dc.creator.none.fl_str_mv Zappacosta, Diego Carlos
Ochogavía, Ana Claudia
Romero, José Rodolfo
Rodrigo, Juan Manuel
Meier, Mauro Sebastián
Polci, Pablo Aldo
Garbus, Ingrid
Pessino, Silvina Claudia
Echenique, Carmen Viviana
author Zappacosta, Diego Carlos
author_facet Zappacosta, Diego Carlos
Ochogavía, Ana Claudia
Romero, José Rodolfo
Rodrigo, Juan Manuel
Meier, Mauro Sebastián
Polci, Pablo Aldo
Garbus, Ingrid
Pessino, Silvina Claudia
Echenique, Carmen Viviana
author_role author
author2 Ochogavía, Ana Claudia
Romero, José Rodolfo
Rodrigo, Juan Manuel
Meier, Mauro Sebastián
Polci, Pablo Aldo
Garbus, Ingrid
Pessino, Silvina Claudia
Echenique, Carmen Viviana
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APOMIXIS
ERAGROSTIS CURVULA
STRESS CONDITIONS
EPIGENETICS
topic APOMIXIS
ERAGROSTIS CURVULA
STRESS CONDITIONS
EPIGENETICS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Over the past few years our research group has been analyzing the molecular mechanisms involved in the reproductive control of weeping lovegrass (Eragrostis curvula [Schrad.] Nees), an apomictic perennial grass. This work was aimed at analyzing apomixis expressivity in Eragrostis curvula plants subjected to three stress conditions: 1) tissue culture followed by a colchicine-induced ploidy change; 2) tissue culture and 3) water deficit. In the first system, a colchicine-induced polyploid plant (C) changed the expressivity of apomixis in a four year period, being highly sexual shortly after polyploidization and highly apomictic four years later. During this interval, genetic and epigenetic changes were also observed. While genetic variation led to diversification with respect to an obligate apomictic control, epigenetic variation resulted in increased similarity. Polymorphic sequences were mainly similar to transposable elements and/or protein-coding-like sequences. In the second system, explants originated from near obligate apomictic plants were subjected to tissue culture. The expressivity of apomixis of a regenerated plant was analyzed immediately after treatment and nine years later. In this period, the levels of sexuality shifted from 45 to 0%. In the third model, near-obligate apomictic plants subjected to water stress showed an increase in expression of sexuality from 2 to 20%. As a whole, our results suggested that stress can temporarily activate sexuality in apomictic genotypes. At least in one system, this change was correlated with genetic and epigenetic modifications involving retroelements and/or protein-coding-like sequences.
Fil: Zappacosta, Diego Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Ochogavía, Ana Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romero, José Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Rodrigo, Juan Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Meier, Mauro Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Polci, Pablo Aldo. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
International Plant and Animal Genome XXII Conference
San Diego
Estados Unidos
Scherago International
description Over the past few years our research group has been analyzing the molecular mechanisms involved in the reproductive control of weeping lovegrass (Eragrostis curvula [Schrad.] Nees), an apomictic perennial grass. This work was aimed at analyzing apomixis expressivity in Eragrostis curvula plants subjected to three stress conditions: 1) tissue culture followed by a colchicine-induced ploidy change; 2) tissue culture and 3) water deficit. In the first system, a colchicine-induced polyploid plant (C) changed the expressivity of apomixis in a four year period, being highly sexual shortly after polyploidization and highly apomictic four years later. During this interval, genetic and epigenetic changes were also observed. While genetic variation led to diversification with respect to an obligate apomictic control, epigenetic variation resulted in increased similarity. Polymorphic sequences were mainly similar to transposable elements and/or protein-coding-like sequences. In the second system, explants originated from near obligate apomictic plants were subjected to tissue culture. The expressivity of apomixis of a regenerated plant was analyzed immediately after treatment and nine years later. In this period, the levels of sexuality shifted from 45 to 0%. In the third model, near-obligate apomictic plants subjected to water stress showed an increase in expression of sexuality from 2 to 20%. As a whole, our results suggested that stress can temporarily activate sexuality in apomictic genotypes. At least in one system, this change was correlated with genetic and epigenetic modifications involving retroelements and/or protein-coding-like sequences.
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/232082
Apomixis expression in Eragrostis curvula under stress conditions; International Plant and Animal Genome XXII Conference; San Diego; Estados Unidos; 2014; 1-1
CONICET Digital
CONICET
url http://hdl.handle.net/11336/232082
identifier_str_mv Apomixis expression in Eragrostis curvula under stress conditions; International Plant and Animal Genome XXII Conference; San Diego; Estados Unidos; 2014; 1-1
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pag.confex.com/pag/xxii/webprogram/Paper10223.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Scherago International
publisher.none.fl_str_mv Scherago International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847978212423892992
score 13.082534

Publicaciones similares