Disclosing the Molecular Link between Apospory and Diplospory

Autores
Garbus, Ingrid; Podio, Maricel; Echenique, Carmen Viviana; Pessino, Silvina Claudia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Gametophytic apomixis forms seeds with clonal embryos identical to the mother plant through two different mechanisms, namely apospory and diplospory, both of them involving the formation of nonreduced megagametophytes within the ovule. These nonreduced embryo sacs are originated from companion nucellar cells (apospory) or the megaspore mother cell, after absence/failure of meiosis (diplospory). In both paths the nonreduced egg cells produce embryos through parthenogenesis. Our hypothesis here was that, at least for some candidate genes, apomeiosis and parthenogenesis associate, respectively, with contrasting or identical representation patterns in aposporous vs. diplosporous differential mRNA expression comparisons. The rationale supporting this proposition is that in aposporous and diplosporous plants apomeiosis might involve some common genes under an opposite spatio-temporal regulation pattern (OE transcripts), while parthenogenesis should be identically activated in both reproductive types (IE candidates). We compared two sets of Roche/454 sequences differentially expressed in reproductive organs of sexual vs. apomictic plants of Paspalum notatum (aposporous) and Eragrostis curvula (diplosporous) from premeiosis to anthesis, to identify OE and IE transcripts. Then, the selected sequences were investigated in TruSeq/Hiseq Illumina RNA floral libraries constructed at different reproductive stages. We found 89 opposite expression transcripts exclusively represented at premeiosis and 66 equal expression transcripts occurring at anthesis only. These candidate genes were used to produce String interaction networks with others previously related with apomeiosis and parthenogenesis, and their location is being mapped within the Paspalum and Eragrostis genomic ACL (apomixis controlling loci). Functional analysis of selected sequences is at work.
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; Argentina
Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
7th Series of Seminars on Advances in Apomixis Research
Argentina
Mechanisms of Apomictic Development
Materia
apomixis controlling loci
diplospory
apospory
Eragrostis
Paspalum
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/182393
Acceder
id CONICETDig_684f2185d2af6302acdd808d2393e70c
oai_identifier_str oai:ri.conicet.gov.ar:11336/182393
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Disclosing the Molecular Link between Apospory and DiplosporyGarbus, IngridPodio, MaricelEchenique, Carmen VivianaPessino, Silvina Claudiaapomixis controlling locidiplosporyaposporyEragrostisPaspalumhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Gametophytic apomixis forms seeds with clonal embryos identical to the mother plant through two different mechanisms, namely apospory and diplospory, both of them involving the formation of nonreduced megagametophytes within the ovule. These nonreduced embryo sacs are originated from companion nucellar cells (apospory) or the megaspore mother cell, after absence/failure of meiosis (diplospory). In both paths the nonreduced egg cells produce embryos through parthenogenesis. Our hypothesis here was that, at least for some candidate genes, apomeiosis and parthenogenesis associate, respectively, with contrasting or identical representation patterns in aposporous vs. diplosporous differential mRNA expression comparisons. The rationale supporting this proposition is that in aposporous and diplosporous plants apomeiosis might involve some common genes under an opposite spatio-temporal regulation pattern (OE transcripts), while parthenogenesis should be identically activated in both reproductive types (IE candidates). We compared two sets of Roche/454 sequences differentially expressed in reproductive organs of sexual vs. apomictic plants of Paspalum notatum (aposporous) and Eragrostis curvula (diplosporous) from premeiosis to anthesis, to identify OE and IE transcripts. Then, the selected sequences were investigated in TruSeq/Hiseq Illumina RNA floral libraries constructed at different reproductive stages. We found 89 opposite expression transcripts exclusively represented at premeiosis and 66 equal expression transcripts occurring at anthesis only. These candidate genes were used to produce String interaction networks with others previously related with apomeiosis and parthenogenesis, and their location is being mapped within the Paspalum and Eragrostis genomic ACL (apomixis controlling loci). Functional analysis of selected sequences is at work.Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; ArgentinaFil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; ArgentinaFil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; ArgentinaFil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina7th Series of Seminars on Advances in Apomixis ResearchArgentinaMechanisms of Apomictic DevelopmentMultidisciplinary Digital Publishing Institute2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectWorkshopJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/182393Disclosing the Molecular Link between Apospory and Diplospory; 7th Series of Seminars on Advances in Apomixis Research; Argentina; 2020; 8-92223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/3/565Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:08Zoai:ri.conicet.gov.ar:11336/182393instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:08.588CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Disclosing the Molecular Link between Apospory and Diplospory
title Disclosing the Molecular Link between Apospory and Diplospory
spellingShingle Disclosing the Molecular Link between Apospory and Diplospory
Garbus, Ingrid
apomixis controlling loci
diplospory
apospory
Eragrostis
Paspalum
title_short Disclosing the Molecular Link between Apospory and Diplospory
title_full Disclosing the Molecular Link between Apospory and Diplospory
title_fullStr Disclosing the Molecular Link between Apospory and Diplospory
title_full_unstemmed Disclosing the Molecular Link between Apospory and Diplospory
title_sort Disclosing the Molecular Link between Apospory and Diplospory
dc.creator.none.fl_str_mv Garbus, Ingrid
Podio, Maricel
Echenique, Carmen Viviana
Pessino, Silvina Claudia
author Garbus, Ingrid
author_facet Garbus, Ingrid
Podio, Maricel
Echenique, Carmen Viviana
Pessino, Silvina Claudia
author_role author
author2 Podio, Maricel
Echenique, Carmen Viviana
Pessino, Silvina Claudia
author2_role author
author
author
dc.subject.none.fl_str_mv apomixis controlling loci
diplospory
apospory
Eragrostis
Paspalum
topic apomixis controlling loci
diplospory
apospory
Eragrostis
Paspalum
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Gametophytic apomixis forms seeds with clonal embryos identical to the mother plant through two different mechanisms, namely apospory and diplospory, both of them involving the formation of nonreduced megagametophytes within the ovule. These nonreduced embryo sacs are originated from companion nucellar cells (apospory) or the megaspore mother cell, after absence/failure of meiosis (diplospory). In both paths the nonreduced egg cells produce embryos through parthenogenesis. Our hypothesis here was that, at least for some candidate genes, apomeiosis and parthenogenesis associate, respectively, with contrasting or identical representation patterns in aposporous vs. diplosporous differential mRNA expression comparisons. The rationale supporting this proposition is that in aposporous and diplosporous plants apomeiosis might involve some common genes under an opposite spatio-temporal regulation pattern (OE transcripts), while parthenogenesis should be identically activated in both reproductive types (IE candidates). We compared two sets of Roche/454 sequences differentially expressed in reproductive organs of sexual vs. apomictic plants of Paspalum notatum (aposporous) and Eragrostis curvula (diplosporous) from premeiosis to anthesis, to identify OE and IE transcripts. Then, the selected sequences were investigated in TruSeq/Hiseq Illumina RNA floral libraries constructed at different reproductive stages. We found 89 opposite expression transcripts exclusively represented at premeiosis and 66 equal expression transcripts occurring at anthesis only. These candidate genes were used to produce String interaction networks with others previously related with apomeiosis and parthenogenesis, and their location is being mapped within the Paspalum and Eragrostis genomic ACL (apomixis controlling loci). Functional analysis of selected sequences is at work.
Fil: Garbus, Ingrid. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Ciencias de la Salud; Argentina
Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario; Argentina
Fil: Echenique, Carmen Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina. Universidad Nacional del Sur. Departamento de Agronomía; Argentina
Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
7th Series of Seminars on Advances in Apomixis Research
Argentina
Mechanisms of Apomictic Development
description Gametophytic apomixis forms seeds with clonal embryos identical to the mother plant through two different mechanisms, namely apospory and diplospory, both of them involving the formation of nonreduced megagametophytes within the ovule. These nonreduced embryo sacs are originated from companion nucellar cells (apospory) or the megaspore mother cell, after absence/failure of meiosis (diplospory). In both paths the nonreduced egg cells produce embryos through parthenogenesis. Our hypothesis here was that, at least for some candidate genes, apomeiosis and parthenogenesis associate, respectively, with contrasting or identical representation patterns in aposporous vs. diplosporous differential mRNA expression comparisons. The rationale supporting this proposition is that in aposporous and diplosporous plants apomeiosis might involve some common genes under an opposite spatio-temporal regulation pattern (OE transcripts), while parthenogenesis should be identically activated in both reproductive types (IE candidates). We compared two sets of Roche/454 sequences differentially expressed in reproductive organs of sexual vs. apomictic plants of Paspalum notatum (aposporous) and Eragrostis curvula (diplosporous) from premeiosis to anthesis, to identify OE and IE transcripts. Then, the selected sequences were investigated in TruSeq/Hiseq Illumina RNA floral libraries constructed at different reproductive stages. We found 89 opposite expression transcripts exclusively represented at premeiosis and 66 equal expression transcripts occurring at anthesis only. These candidate genes were used to produce String interaction networks with others previously related with apomeiosis and parthenogenesis, and their location is being mapped within the Paspalum and Eragrostis genomic ACL (apomixis controlling loci). Functional analysis of selected sequences is at work.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Workshop
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/182393
Disclosing the Molecular Link between Apospory and Diplospory; 7th Series of Seminars on Advances in Apomixis Research; Argentina; 2020; 8-9
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/182393
identifier_str_mv Disclosing the Molecular Link between Apospory and Diplospory; 7th Series of Seminars on Advances in Apomixis Research; Argentina; 2020; 8-9
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2223-7747/10/3/565
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269739629412352
score 13.13397

Publicaciones similares