Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670

Autores
Yashchuk, Oxana; Miyazaki, Silvia Susana; Hermida, Elida Beatriz
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
–Streptomyces sp. SSM 5670 with the ability to degrade a natural polymers, polyhydroxyalkanoates (PHAs), showed a high activity of the extracellular poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) depolymerase when it was grown in a basal medium containing PHBV as the sole carbon source. The extracellular PHBV depolymerase of the organism could be purified to electrophoretic homogeneity by ion exchange column chromatography and gel filtration approximately 10.5-fold. The recovery of its activity was 20.5%. The molecular mass of the PHBV depolymerase was estimated to be 24 kDa. The maximum activity was observed near pH 7.5 and 30°C, the activity was lost at temperatures above 45°C. This enzyme might be used to promote the degradation of PHBV products buried in soil, stabilized compost or other environments around room temperature.
Fil: Yashchuk, Oxana. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miyazaki, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos. Area de Agroalimentos; Argentina
Fil: Hermida, Elida Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Materia
aislamiento
purificación
PHB depolimerasa
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/104281
Acceder
id CONICETDig_fb2d56857e29b088b30212001ad21083
oai_identifier_str oai:ri.conicet.gov.ar:11336/104281
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670Yashchuk, OxanaMiyazaki, Silvia SusanaHermida, Elida BeatrizaislamientopurificaciónPHB depolimerasahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1–Streptomyces sp. SSM 5670 with the ability to degrade a natural polymers, polyhydroxyalkanoates (PHAs), showed a high activity of the extracellular poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) depolymerase when it was grown in a basal medium containing PHBV as the sole carbon source. The extracellular PHBV depolymerase of the organism could be purified to electrophoretic homogeneity by ion exchange column chromatography and gel filtration approximately 10.5-fold. The recovery of its activity was 20.5%. The molecular mass of the PHBV depolymerase was estimated to be 24 kDa. The maximum activity was observed near pH 7.5 and 30°C, the activity was lost at temperatures above 45°C. This enzyme might be used to promote the degradation of PHBV products buried in soil, stabilized compost or other environments around room temperature.Fil: Yashchuk, Oxana. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Miyazaki, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos. Area de Agroalimentos; ArgentinaFil: Hermida, Elida Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaGlobal Science Publications Aligarh2014-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/104281Yashchuk, Oxana; Miyazaki, Silvia Susana; Hermida, Elida Beatriz; Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670; Global Science Publications Aligarh; Asian Journal of Microbiology, Biotechnology & Environmental Sciences; 16; 1; 1-2014; 29-330972-3005CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.envirobiotechjournals.com/article_abstract.php?aid=4868&iid=167&jid=1info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:44:12Zoai:ri.conicet.gov.ar:11336/104281instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:44:12.709CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670
title Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670
spellingShingle Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670
Yashchuk, Oxana
aislamiento
purificación
PHB depolimerasa
title_short Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670
title_full Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670
title_fullStr Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670
title_full_unstemmed Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670
title_sort Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670
dc.creator.none.fl_str_mv Yashchuk, Oxana
Miyazaki, Silvia Susana
Hermida, Elida Beatriz
author Yashchuk, Oxana
author_facet Yashchuk, Oxana
Miyazaki, Silvia Susana
Hermida, Elida Beatriz
author_role author
author2 Miyazaki, Silvia Susana
Hermida, Elida Beatriz
author2_role author
author
dc.subject.none.fl_str_mv aislamiento
purificación
PHB depolimerasa
topic aislamiento
purificación
PHB depolimerasa
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv –Streptomyces sp. SSM 5670 with the ability to degrade a natural polymers, polyhydroxyalkanoates (PHAs), showed a high activity of the extracellular poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) depolymerase when it was grown in a basal medium containing PHBV as the sole carbon source. The extracellular PHBV depolymerase of the organism could be purified to electrophoretic homogeneity by ion exchange column chromatography and gel filtration approximately 10.5-fold. The recovery of its activity was 20.5%. The molecular mass of the PHBV depolymerase was estimated to be 24 kDa. The maximum activity was observed near pH 7.5 and 30°C, the activity was lost at temperatures above 45°C. This enzyme might be used to promote the degradation of PHBV products buried in soil, stabilized compost or other environments around room temperature.
Fil: Yashchuk, Oxana. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miyazaki, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Biología Aplicada y Alimentos. Area de Agroalimentos; Argentina
Fil: Hermida, Elida Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
description –Streptomyces sp. SSM 5670 with the ability to degrade a natural polymers, polyhydroxyalkanoates (PHAs), showed a high activity of the extracellular poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) depolymerase when it was grown in a basal medium containing PHBV as the sole carbon source. The extracellular PHBV depolymerase of the organism could be purified to electrophoretic homogeneity by ion exchange column chromatography and gel filtration approximately 10.5-fold. The recovery of its activity was 20.5%. The molecular mass of the PHBV depolymerase was estimated to be 24 kDa. The maximum activity was observed near pH 7.5 and 30°C, the activity was lost at temperatures above 45°C. This enzyme might be used to promote the degradation of PHBV products buried in soil, stabilized compost or other environments around room temperature.
publishDate 2014
dc.date.none.fl_str_mv 2014-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/104281
Yashchuk, Oxana; Miyazaki, Silvia Susana; Hermida, Elida Beatriz; Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670; Global Science Publications Aligarh; Asian Journal of Microbiology, Biotechnology & Environmental Sciences; 16; 1; 1-2014; 29-33
0972-3005
CONICET Digital
CONICET
url http://hdl.handle.net/11336/104281
identifier_str_mv Yashchuk, Oxana; Miyazaki, Silvia Susana; Hermida, Elida Beatriz; Isolation, Purification and Some Properties of an Extracellular PHBV Depolymerase from Streptomyces omiyaensis SSM 5670; Global Science Publications Aligarh; Asian Journal of Microbiology, Biotechnology & Environmental Sciences; 16; 1; 1-2014; 29-33
0972-3005
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.envirobiotechjournals.com/article_abstract.php?aid=4868&iid=167&jid=1
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Global Science Publications Aligarh
publisher.none.fl_str_mv Global Science Publications Aligarh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614479135899648
score 13.069144

Publicaciones similares