Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance

Autores
Jaime, Camila Lourdes; Dezar, Carlos Alberto Alejandro; Pagán, Israel; Dunger, Ricardo German
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ascorbate-glutathione pathway plays an essential role in the physiology of vascu-lar plants, particularly in their response to environmental stresses. This pathway isresponsible for regulating the cellular redox state, which is critical for maintaining cellfunction and survival under adverse conditions. To study the involvement of thealfalfa monodehydroascorbate reductase (MsMDHAR) in water stress processes, Ara-bidopsis thaliana plants constitutively expressing the sequence encoding MsMDHARwere developed. Transgenic events with low and high MsMDHAR expression andascorbate levels were selected for further analysis of drought and waterlogging toler-ance. Under water stress, Arabidopsis transgenic plants generated higher biomass,produced more seeds, and had larger roots than wild type ones. This higher tolerancewas associated with increased production of waxes and chlorophyll a at the basallevel, greater stomatal opening and stability in regulating the relative water contentand reduced H 2O 2 accumulation under stress conditions in transgenic plants. Overall,these results show that MsMDHAR is involved in plant tolerance to abiotic stresses.The data presented here also emphasises the potential of the MsMDHAR enzyme asa plant breeding tool to improve water stress tolerance.
Fil: Jaime, Camila Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Fil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Fil: Pagán, Israel. Universidad Politécnica de Madrid; España
Fil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Materia
ALFALFA
MSMDHAR
WATER STRESS
BIOMASS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/256568
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress toleranceJaime, Camila LourdesDezar, Carlos Alberto AlejandroPagán, IsraelDunger, Ricardo GermanALFALFAMSMDHARWATER STRESSBIOMASShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The ascorbate-glutathione pathway plays an essential role in the physiology of vascu-lar plants, particularly in their response to environmental stresses. This pathway isresponsible for regulating the cellular redox state, which is critical for maintaining cellfunction and survival under adverse conditions. To study the involvement of thealfalfa monodehydroascorbate reductase (MsMDHAR) in water stress processes, Ara-bidopsis thaliana plants constitutively expressing the sequence encoding MsMDHARwere developed. Transgenic events with low and high MsMDHAR expression andascorbate levels were selected for further analysis of drought and waterlogging toler-ance. Under water stress, Arabidopsis transgenic plants generated higher biomass,produced more seeds, and had larger roots than wild type ones. This higher tolerancewas associated with increased production of waxes and chlorophyll a at the basallevel, greater stomatal opening and stability in regulating the relative water contentand reduced H 2O 2 accumulation under stress conditions in transgenic plants. Overall,these results show that MsMDHAR is involved in plant tolerance to abiotic stresses.The data presented here also emphasises the potential of the MsMDHAR enzyme asa plant breeding tool to improve water stress tolerance.Fil: Jaime, Camila Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; ArgentinaFil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; ArgentinaFil: Pagán, Israel. Universidad Politécnica de Madrid; EspañaFil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; ArgentinaWiley Blackwell Publishing, Inc2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/256568Jaime, Camila Lourdes; Dezar, Carlos Alberto Alejandro; Pagán, Israel; Dunger, Ricardo German; Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance; Wiley Blackwell Publishing, Inc; Physiologia Plantarum; 176; 4; 7-2024; 1-150031-9317CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/ppl.14448info:eu-repo/semantics/altIdentifier/doi/10.1111/ppl.14448info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:23Zoai:ri.conicet.gov.ar:11336/256568instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:24.212CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance
title Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance
spellingShingle Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance
Jaime, Camila Lourdes
ALFALFA
MSMDHAR
WATER STRESS
BIOMASS
title_short Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance
title_full Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance
title_fullStr Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance
title_full_unstemmed Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance
title_sort Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance
dc.creator.none.fl_str_mv Jaime, Camila Lourdes
Dezar, Carlos Alberto Alejandro
Pagán, Israel
Dunger, Ricardo German
author Jaime, Camila Lourdes
author_facet Jaime, Camila Lourdes
Dezar, Carlos Alberto Alejandro
Pagán, Israel
Dunger, Ricardo German
author_role author
author2 Dezar, Carlos Alberto Alejandro
Pagán, Israel
Dunger, Ricardo German
author2_role author
author
author
dc.subject.none.fl_str_mv ALFALFA
MSMDHAR
WATER STRESS
BIOMASS
topic ALFALFA
MSMDHAR
WATER STRESS
BIOMASS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The ascorbate-glutathione pathway plays an essential role in the physiology of vascu-lar plants, particularly in their response to environmental stresses. This pathway isresponsible for regulating the cellular redox state, which is critical for maintaining cellfunction and survival under adverse conditions. To study the involvement of thealfalfa monodehydroascorbate reductase (MsMDHAR) in water stress processes, Ara-bidopsis thaliana plants constitutively expressing the sequence encoding MsMDHARwere developed. Transgenic events with low and high MsMDHAR expression andascorbate levels were selected for further analysis of drought and waterlogging toler-ance. Under water stress, Arabidopsis transgenic plants generated higher biomass,produced more seeds, and had larger roots than wild type ones. This higher tolerancewas associated with increased production of waxes and chlorophyll a at the basallevel, greater stomatal opening and stability in regulating the relative water contentand reduced H 2O 2 accumulation under stress conditions in transgenic plants. Overall,these results show that MsMDHAR is involved in plant tolerance to abiotic stresses.The data presented here also emphasises the potential of the MsMDHAR enzyme asa plant breeding tool to improve water stress tolerance.
Fil: Jaime, Camila Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Fil: Dezar, Carlos Alberto Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
Fil: Pagán, Israel. Universidad Politécnica de Madrid; España
Fil: Dunger, Ricardo German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Agropecuarias del Litoral. Universidad Nacional del Litoral. Instituto de Ciencias Agropecuarias del Litoral; Argentina
description The ascorbate-glutathione pathway plays an essential role in the physiology of vascu-lar plants, particularly in their response to environmental stresses. This pathway isresponsible for regulating the cellular redox state, which is critical for maintaining cellfunction and survival under adverse conditions. To study the involvement of thealfalfa monodehydroascorbate reductase (MsMDHAR) in water stress processes, Ara-bidopsis thaliana plants constitutively expressing the sequence encoding MsMDHARwere developed. Transgenic events with low and high MsMDHAR expression andascorbate levels were selected for further analysis of drought and waterlogging toler-ance. Under water stress, Arabidopsis transgenic plants generated higher biomass,produced more seeds, and had larger roots than wild type ones. This higher tolerancewas associated with increased production of waxes and chlorophyll a at the basallevel, greater stomatal opening and stability in regulating the relative water contentand reduced H 2O 2 accumulation under stress conditions in transgenic plants. Overall,these results show that MsMDHAR is involved in plant tolerance to abiotic stresses.The data presented here also emphasises the potential of the MsMDHAR enzyme asa plant breeding tool to improve water stress tolerance.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/256568
Jaime, Camila Lourdes; Dezar, Carlos Alberto Alejandro; Pagán, Israel; Dunger, Ricardo German; Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance; Wiley Blackwell Publishing, Inc; Physiologia Plantarum; 176; 4; 7-2024; 1-15
0031-9317
CONICET Digital
CONICET
url http://hdl.handle.net/11336/256568
identifier_str_mv Jaime, Camila Lourdes; Dezar, Carlos Alberto Alejandro; Pagán, Israel; Dunger, Ricardo German; Expression of the alfalfa gene MsMDHAR in Arabidopsis thaliana increases water stress tolerance; Wiley Blackwell Publishing, Inc; Physiologia Plantarum; 176; 4; 7-2024; 1-15
0031-9317
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/ppl.14448
info:eu-repo/semantics/altIdentifier/doi/10.1111/ppl.14448
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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