Cellular Remodelling by Apoptosis During Porcine Placentation

Autores
Cristofolini, Andrea Lorena; Sanchis, Eva Gabriela; Moliva, Melina Vanesa; Alonso, L.; Chanique, A.; Koncurat, Mirta Adriana; Merkis, Cecilia Inés
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The mechanisms that regulate the apoptosis are essential to the normal development and maintenance of homoeostasis and play an important role in placental development in mammals. During porcine pregnancy, there must be a proper cellular remodelling to achieve a normal gestational development. Knowledge of pig physiology during pregnancy will explore options to increase the productivity of this species of high economical value. The purpose of this work was to study the cell morphology and apoptosis of porcine placentas from early, mid and late pregnancy. For that purpose, highresolution light microscopy and transmission electron microscopy were performed to the study of cell morphology. TUNEL, the apoptosis index (IAp) and the expression of c-FLIP through immunohistochemistry technique were used to the study of apoptosis. High-resolution light microscopy and transmission electron microscopy confirmed the presence of placental cells with ultrastructural apoptotic features. Apoptotic nuclei were detected by TUNEL in different placental structures and phagocytes containing apoptotic bodies. The IAp in villi was 9.34% at early, 0.82% at mid and 23.85% at late pregnancy. Statistically significant differences were found between periods (p < 0.05). In previous studies, we determined a differential induction of the apoptotic routes in the placental villi in agreement with the gestational period. A co-expression of receptors and mitochondrial proteins in placental connective tissue was detected, but the immunolocalization of c-FLIP would indicate an endogenous inhibition of the extrinsic pathway. In conclusion, in swine there exists differential activation of inducing apoptotic pathways in different placental structures according to the gestational period.
Fil: Cristofolini, Andrea Lorena. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchis, Eva Gabriela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moliva, Melina Vanesa. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, L.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Chanique, A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Koncurat, Mirta Adriana. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Materia
Porcine
Placenta
Apoptosis
Cell Remodelling
Apoptotic Pathways
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/22698
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/22698
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cellular Remodelling by Apoptosis During Porcine PlacentationCristofolini, Andrea LorenaSanchis, Eva GabrielaMoliva, Melina VanesaAlonso, L.Chanique, A.Koncurat, Mirta AdrianaMerkis, Cecilia InésPorcinePlacentaApoptosisCell RemodellingApoptotic Pathwayshttps://purl.org/becyt/ford/4.2https://purl.org/becyt/ford/4https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The mechanisms that regulate the apoptosis are essential to the normal development and maintenance of homoeostasis and play an important role in placental development in mammals. During porcine pregnancy, there must be a proper cellular remodelling to achieve a normal gestational development. Knowledge of pig physiology during pregnancy will explore options to increase the productivity of this species of high economical value. The purpose of this work was to study the cell morphology and apoptosis of porcine placentas from early, mid and late pregnancy. For that purpose, highresolution light microscopy and transmission electron microscopy were performed to the study of cell morphology. TUNEL, the apoptosis index (IAp) and the expression of c-FLIP through immunohistochemistry technique were used to the study of apoptosis. High-resolution light microscopy and transmission electron microscopy confirmed the presence of placental cells with ultrastructural apoptotic features. Apoptotic nuclei were detected by TUNEL in different placental structures and phagocytes containing apoptotic bodies. The IAp in villi was 9.34% at early, 0.82% at mid and 23.85% at late pregnancy. Statistically significant differences were found between periods (p < 0.05). In previous studies, we determined a differential induction of the apoptotic routes in the placental villi in agreement with the gestational period. A co-expression of receptors and mitochondrial proteins in placental connective tissue was detected, but the immunolocalization of c-FLIP would indicate an endogenous inhibition of the extrinsic pathway. In conclusion, in swine there exists differential activation of inducing apoptotic pathways in different placental structures according to the gestational period.Fil: Cristofolini, Andrea Lorena. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchis, Eva Gabriela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moliva, Melina Vanesa. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso, L.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Chanique, A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Koncurat, Mirta Adriana. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaWiley VCH Verlag2013-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/22698Cristofolini, Andrea Lorena; Sanchis, Eva Gabriela; Moliva, Melina Vanesa; Alonso, L.; Chanique, A.; et al.; Cellular Remodelling by Apoptosis During Porcine Placentation; Wiley VCH Verlag; Reproduction in Domestic Animals; 48; 4; 2-2013; 584-5900936-6768CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12130info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12130/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:28:39Zoai:ri.conicet.gov.ar:11336/22698instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:28:39.775CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cellular Remodelling by Apoptosis During Porcine Placentation
title Cellular Remodelling by Apoptosis During Porcine Placentation
spellingShingle Cellular Remodelling by Apoptosis During Porcine Placentation
Cristofolini, Andrea Lorena
Porcine
Placenta
Apoptosis
Cell Remodelling
Apoptotic Pathways
title_short Cellular Remodelling by Apoptosis During Porcine Placentation
title_full Cellular Remodelling by Apoptosis During Porcine Placentation
title_fullStr Cellular Remodelling by Apoptosis During Porcine Placentation
title_full_unstemmed Cellular Remodelling by Apoptosis During Porcine Placentation
title_sort Cellular Remodelling by Apoptosis During Porcine Placentation
dc.creator.none.fl_str_mv Cristofolini, Andrea Lorena
Sanchis, Eva Gabriela
Moliva, Melina Vanesa
Alonso, L.
Chanique, A.
Koncurat, Mirta Adriana
Merkis, Cecilia Inés
author Cristofolini, Andrea Lorena
author_facet Cristofolini, Andrea Lorena
Sanchis, Eva Gabriela
Moliva, Melina Vanesa
Alonso, L.
Chanique, A.
Koncurat, Mirta Adriana
Merkis, Cecilia Inés
author_role author
author2 Sanchis, Eva Gabriela
Moliva, Melina Vanesa
Alonso, L.
Chanique, A.
Koncurat, Mirta Adriana
Merkis, Cecilia Inés
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Porcine
Placenta
Apoptosis
Cell Remodelling
Apoptotic Pathways
topic Porcine
Placenta
Apoptosis
Cell Remodelling
Apoptotic Pathways
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.2
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The mechanisms that regulate the apoptosis are essential to the normal development and maintenance of homoeostasis and play an important role in placental development in mammals. During porcine pregnancy, there must be a proper cellular remodelling to achieve a normal gestational development. Knowledge of pig physiology during pregnancy will explore options to increase the productivity of this species of high economical value. The purpose of this work was to study the cell morphology and apoptosis of porcine placentas from early, mid and late pregnancy. For that purpose, highresolution light microscopy and transmission electron microscopy were performed to the study of cell morphology. TUNEL, the apoptosis index (IAp) and the expression of c-FLIP through immunohistochemistry technique were used to the study of apoptosis. High-resolution light microscopy and transmission electron microscopy confirmed the presence of placental cells with ultrastructural apoptotic features. Apoptotic nuclei were detected by TUNEL in different placental structures and phagocytes containing apoptotic bodies. The IAp in villi was 9.34% at early, 0.82% at mid and 23.85% at late pregnancy. Statistically significant differences were found between periods (p < 0.05). In previous studies, we determined a differential induction of the apoptotic routes in the placental villi in agreement with the gestational period. A co-expression of receptors and mitochondrial proteins in placental connective tissue was detected, but the immunolocalization of c-FLIP would indicate an endogenous inhibition of the extrinsic pathway. In conclusion, in swine there exists differential activation of inducing apoptotic pathways in different placental structures according to the gestational period.
Fil: Cristofolini, Andrea Lorena. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchis, Eva Gabriela. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moliva, Melina Vanesa. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso, L.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Chanique, A.. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Koncurat, Mirta Adriana. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Merkis, Cecilia Inés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
description The mechanisms that regulate the apoptosis are essential to the normal development and maintenance of homoeostasis and play an important role in placental development in mammals. During porcine pregnancy, there must be a proper cellular remodelling to achieve a normal gestational development. Knowledge of pig physiology during pregnancy will explore options to increase the productivity of this species of high economical value. The purpose of this work was to study the cell morphology and apoptosis of porcine placentas from early, mid and late pregnancy. For that purpose, highresolution light microscopy and transmission electron microscopy were performed to the study of cell morphology. TUNEL, the apoptosis index (IAp) and the expression of c-FLIP through immunohistochemistry technique were used to the study of apoptosis. High-resolution light microscopy and transmission electron microscopy confirmed the presence of placental cells with ultrastructural apoptotic features. Apoptotic nuclei were detected by TUNEL in different placental structures and phagocytes containing apoptotic bodies. The IAp in villi was 9.34% at early, 0.82% at mid and 23.85% at late pregnancy. Statistically significant differences were found between periods (p < 0.05). In previous studies, we determined a differential induction of the apoptotic routes in the placental villi in agreement with the gestational period. A co-expression of receptors and mitochondrial proteins in placental connective tissue was detected, but the immunolocalization of c-FLIP would indicate an endogenous inhibition of the extrinsic pathway. In conclusion, in swine there exists differential activation of inducing apoptotic pathways in different placental structures according to the gestational period.
publishDate 2013
dc.date.none.fl_str_mv 2013-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/22698
Cristofolini, Andrea Lorena; Sanchis, Eva Gabriela; Moliva, Melina Vanesa; Alonso, L.; Chanique, A.; et al.; Cellular Remodelling by Apoptosis During Porcine Placentation; Wiley VCH Verlag; Reproduction in Domestic Animals; 48; 4; 2-2013; 584-590
0936-6768
CONICET Digital
CONICET
url http://hdl.handle.net/11336/22698
identifier_str_mv Cristofolini, Andrea Lorena; Sanchis, Eva Gabriela; Moliva, Melina Vanesa; Alonso, L.; Chanique, A.; et al.; Cellular Remodelling by Apoptosis During Porcine Placentation; Wiley VCH Verlag; Reproduction in Domestic Animals; 48; 4; 2-2013; 584-590
0936-6768
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/rda.12130
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/rda.12130/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley VCH Verlag
publisher.none.fl_str_mv Wiley VCH Verlag
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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