Evaluation of the toxic activity of Zinnia peruviana products

Autores
Mohamed, Ana Mariel; Escobar, Franco Matias; Torres, Cristina; Satorres, Sara Elena; Cifuente, Diego Alberto; Mattana, Claudia Maricel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although plants have been used in popular medicine for ages, their safety is often not well studied, and many can be toxic. In this study, the toxicity of Zinnia peruviana products was evaluated through cytotoxicity and genotoxicity assays. Neutral Red (NR) uptake and MTT reduction were used to measure cell growth inhibition by these products. Genetic damage was evaluated using the comet assay. Acetonic extracts of roots, leaves and flowers and ziniolide (major metabolite of the roots extract), were evaluated. The flowers and roots extracts, as well as the ziniolide did not present significant cytotoxic effects on Vero cells using neutral red and MTT assays (CC50> 20 µg/mL). These products also did not cause DNA damage at the lowest concentration tested (0.156 mg/mL). Significant genotoxic damage was observed at concentrations equal to or greater than 1.25 mg/mL, following a dose-dependent behavior. However, the leaves extract showed cytotoxic activity with NR and MTT (CC50 = 18 µg/mL and CC50= 15 µg/mL respectively) and also genotoxicity at low concentration evaluated in previous studies. The results of this research are promising for advancing studies in search of the bioapplicability of these natural compounds as antimicrobial agents.
Fil: Mohamed, Ana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Escobar, Franco Matias. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Torres, Cristina. Universidad Nacional de Río Cuarto; Argentina
Fil: Satorres, Sara Elena. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cifuente, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Mattana, Claudia Maricel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Materia
CYTOTOXICITY
GENOTOXICITY
ZINNIA PERUVIANA
ZINIOLIDE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266785
Acceder
id CONICETDig_fab186bdb2678fe6602c9accef05c531
oai_identifier_str oai:ri.conicet.gov.ar:11336/266785
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the toxic activity of Zinnia peruviana productsMohamed, Ana MarielEscobar, Franco MatiasTorres, CristinaSatorres, Sara ElenaCifuente, Diego AlbertoMattana, Claudia MaricelCYTOTOXICITYGENOTOXICITYZINNIA PERUVIANAZINIOLIDEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Although plants have been used in popular medicine for ages, their safety is often not well studied, and many can be toxic. In this study, the toxicity of Zinnia peruviana products was evaluated through cytotoxicity and genotoxicity assays. Neutral Red (NR) uptake and MTT reduction were used to measure cell growth inhibition by these products. Genetic damage was evaluated using the comet assay. Acetonic extracts of roots, leaves and flowers and ziniolide (major metabolite of the roots extract), were evaluated. The flowers and roots extracts, as well as the ziniolide did not present significant cytotoxic effects on Vero cells using neutral red and MTT assays (CC50> 20 µg/mL). These products also did not cause DNA damage at the lowest concentration tested (0.156 mg/mL). Significant genotoxic damage was observed at concentrations equal to or greater than 1.25 mg/mL, following a dose-dependent behavior. However, the leaves extract showed cytotoxic activity with NR and MTT (CC50 = 18 µg/mL and CC50= 15 µg/mL respectively) and also genotoxicity at low concentration evaluated in previous studies. The results of this research are promising for advancing studies in search of the bioapplicability of these natural compounds as antimicrobial agents.Fil: Mohamed, Ana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Escobar, Franco Matias. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; ArgentinaFil: Torres, Cristina. Universidad Nacional de Río Cuarto; ArgentinaFil: Satorres, Sara Elena. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Cifuente, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; ArgentinaFil: Mattana, Claudia Maricel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaMerit Research Journal2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266785Mohamed, Ana Mariel; Escobar, Franco Matias; Torres, Cristina; Satorres, Sara Elena; Cifuente, Diego Alberto; et al.; Evaluation of the toxic activity of Zinnia peruviana products; Merit Research Journal; Merit Research Journal of Microbiology and Biological Sciences; 11; 1; 7-2024; 1-102408-7076CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://meritresearchjournals.org/articles/482908072024info:eu-repo/semantics/altIdentifier/doi/10.5281/zenodo.12685692info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:28Zoai:ri.conicet.gov.ar:11336/266785instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:28.439CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the toxic activity of Zinnia peruviana products
title Evaluation of the toxic activity of Zinnia peruviana products
spellingShingle Evaluation of the toxic activity of Zinnia peruviana products
Mohamed, Ana Mariel
CYTOTOXICITY
GENOTOXICITY
ZINNIA PERUVIANA
ZINIOLIDE
title_short Evaluation of the toxic activity of Zinnia peruviana products
title_full Evaluation of the toxic activity of Zinnia peruviana products
title_fullStr Evaluation of the toxic activity of Zinnia peruviana products
title_full_unstemmed Evaluation of the toxic activity of Zinnia peruviana products
title_sort Evaluation of the toxic activity of Zinnia peruviana products
dc.creator.none.fl_str_mv Mohamed, Ana Mariel
Escobar, Franco Matias
Torres, Cristina
Satorres, Sara Elena
Cifuente, Diego Alberto
Mattana, Claudia Maricel
author Mohamed, Ana Mariel
author_facet Mohamed, Ana Mariel
Escobar, Franco Matias
Torres, Cristina
Satorres, Sara Elena
Cifuente, Diego Alberto
Mattana, Claudia Maricel
author_role author
author2 Escobar, Franco Matias
Torres, Cristina
Satorres, Sara Elena
Cifuente, Diego Alberto
Mattana, Claudia Maricel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CYTOTOXICITY
GENOTOXICITY
ZINNIA PERUVIANA
ZINIOLIDE
topic CYTOTOXICITY
GENOTOXICITY
ZINNIA PERUVIANA
ZINIOLIDE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Although plants have been used in popular medicine for ages, their safety is often not well studied, and many can be toxic. In this study, the toxicity of Zinnia peruviana products was evaluated through cytotoxicity and genotoxicity assays. Neutral Red (NR) uptake and MTT reduction were used to measure cell growth inhibition by these products. Genetic damage was evaluated using the comet assay. Acetonic extracts of roots, leaves and flowers and ziniolide (major metabolite of the roots extract), were evaluated. The flowers and roots extracts, as well as the ziniolide did not present significant cytotoxic effects on Vero cells using neutral red and MTT assays (CC50> 20 µg/mL). These products also did not cause DNA damage at the lowest concentration tested (0.156 mg/mL). Significant genotoxic damage was observed at concentrations equal to or greater than 1.25 mg/mL, following a dose-dependent behavior. However, the leaves extract showed cytotoxic activity with NR and MTT (CC50 = 18 µg/mL and CC50= 15 µg/mL respectively) and also genotoxicity at low concentration evaluated in previous studies. The results of this research are promising for advancing studies in search of the bioapplicability of these natural compounds as antimicrobial agents.
Fil: Mohamed, Ana Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Escobar, Franco Matias. Universidad Nacional de Rio Cuarto. Facultad de Agronomia y Veterinaria. Instituto de Ciencias Veterinarias. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Instituto de Ciencias Veterinarias.; Argentina
Fil: Torres, Cristina. Universidad Nacional de Río Cuarto; Argentina
Fil: Satorres, Sara Elena. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Cifuente, Diego Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Investigaciones en Tecnología Química. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Investigaciones en Tecnología Química; Argentina
Fil: Mattana, Claudia Maricel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
description Although plants have been used in popular medicine for ages, their safety is often not well studied, and many can be toxic. In this study, the toxicity of Zinnia peruviana products was evaluated through cytotoxicity and genotoxicity assays. Neutral Red (NR) uptake and MTT reduction were used to measure cell growth inhibition by these products. Genetic damage was evaluated using the comet assay. Acetonic extracts of roots, leaves and flowers and ziniolide (major metabolite of the roots extract), were evaluated. The flowers and roots extracts, as well as the ziniolide did not present significant cytotoxic effects on Vero cells using neutral red and MTT assays (CC50> 20 µg/mL). These products also did not cause DNA damage at the lowest concentration tested (0.156 mg/mL). Significant genotoxic damage was observed at concentrations equal to or greater than 1.25 mg/mL, following a dose-dependent behavior. However, the leaves extract showed cytotoxic activity with NR and MTT (CC50 = 18 µg/mL and CC50= 15 µg/mL respectively) and also genotoxicity at low concentration evaluated in previous studies. The results of this research are promising for advancing studies in search of the bioapplicability of these natural compounds as antimicrobial agents.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266785
Mohamed, Ana Mariel; Escobar, Franco Matias; Torres, Cristina; Satorres, Sara Elena; Cifuente, Diego Alberto; et al.; Evaluation of the toxic activity of Zinnia peruviana products; Merit Research Journal; Merit Research Journal of Microbiology and Biological Sciences; 11; 1; 7-2024; 1-10
2408-7076
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266785
identifier_str_mv Mohamed, Ana Mariel; Escobar, Franco Matias; Torres, Cristina; Satorres, Sara Elena; Cifuente, Diego Alberto; et al.; Evaluation of the toxic activity of Zinnia peruviana products; Merit Research Journal; Merit Research Journal of Microbiology and Biological Sciences; 11; 1; 7-2024; 1-10
2408-7076
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://meritresearchjournals.org/articles/482908072024
info:eu-repo/semantics/altIdentifier/doi/10.5281/zenodo.12685692
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Merit Research Journal
publisher.none.fl_str_mv Merit Research Journal
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844614032827351040
score 13.070432

Publicaciones similares