Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America

Autores
Dominguez Valenzuela, José A.; Palma Bautista, Candelario; Vázquez García, José G.; Yanniccari, Marcos Ezequiel; Gigón, Ramón; Alcántara de la Cruz, Ricardo; De Prado, Rafael; Portugal, Joao
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Herbicide-resistant weeds have been identified and recorded on every continent wherecroplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.
Fil: Dominguez Valenzuela, José A.. Universidad Autonoma de Chapingo; México
Fil: Palma Bautista, Candelario. Universidad de Córdoba; España
Fil: Vázquez García, José G.. Universidad de Córdoba; España
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa; Argentina
Fil: Gigón, Ramón. Asesor Privado; Argentina
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; Brasil
Fil: De Prado, Rafael. Universidad de Córdoba; España
Fil: Portugal, Joao. Instituto Politecnico de Beja; Portugal
Materia
ALS ENZYME ACTIVITY
ETHYLENE PRODUCTION
HERBICIDE TOLERANT CROPS
OILSEED RAPE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237261
Acceder
id CONICETDig_fa5226e88a62628888a1a5b2fa9fcf78
oai_identifier_str oai:ri.conicet.gov.ar:11336/237261
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South AmericaDominguez Valenzuela, José A.Palma Bautista, CandelarioVázquez García, José G.Yanniccari, Marcos EzequielGigón, RamónAlcántara de la Cruz, RicardoDe Prado, RafaelPortugal, JoaoALS ENZYME ACTIVITYETHYLENE PRODUCTIONHERBICIDE TOLERANT CROPSOILSEED RAPEhttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4Herbicide-resistant weeds have been identified and recorded on every continent wherecroplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.Fil: Dominguez Valenzuela, José A.. Universidad Autonoma de Chapingo; MéxicoFil: Palma Bautista, Candelario. Universidad de Córdoba; EspañaFil: Vázquez García, José G.. Universidad de Córdoba; EspañaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa; ArgentinaFil: Gigón, Ramón. Asesor Privado; ArgentinaFil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; BrasilFil: De Prado, Rafael. Universidad de Córdoba; EspañaFil: Portugal, Joao. Instituto Politecnico de Beja; PortugalMDPI2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237261Dominguez Valenzuela, José A.; Palma Bautista, Candelario; Vázquez García, José G.; Yanniccari, Marcos Ezequiel; Gigón, Ramón; et al.; Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America; MDPI; Plants; 12; 11; 5-2023; 1-112223-7747CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/plants12112119info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:29:31Zoai:ri.conicet.gov.ar:11336/237261instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:29:31.33CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
spellingShingle Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
Dominguez Valenzuela, José A.
ALS ENZYME ACTIVITY
ETHYLENE PRODUCTION
HERBICIDE TOLERANT CROPS
OILSEED RAPE
title_short Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title_full Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title_fullStr Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title_full_unstemmed Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title_sort Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
dc.creator.none.fl_str_mv Dominguez Valenzuela, José A.
Palma Bautista, Candelario
Vázquez García, José G.
Yanniccari, Marcos Ezequiel
Gigón, Ramón
Alcántara de la Cruz, Ricardo
De Prado, Rafael
Portugal, Joao
author Dominguez Valenzuela, José A.
author_facet Dominguez Valenzuela, José A.
Palma Bautista, Candelario
Vázquez García, José G.
Yanniccari, Marcos Ezequiel
Gigón, Ramón
Alcántara de la Cruz, Ricardo
De Prado, Rafael
Portugal, Joao
author_role author
author2 Palma Bautista, Candelario
Vázquez García, José G.
Yanniccari, Marcos Ezequiel
Gigón, Ramón
Alcántara de la Cruz, Ricardo
De Prado, Rafael
Portugal, Joao
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALS ENZYME ACTIVITY
ETHYLENE PRODUCTION
HERBICIDE TOLERANT CROPS
OILSEED RAPE
topic ALS ENZYME ACTIVITY
ETHYLENE PRODUCTION
HERBICIDE TOLERANT CROPS
OILSEED RAPE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Herbicide-resistant weeds have been identified and recorded on every continent wherecroplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.
Fil: Dominguez Valenzuela, José A.. Universidad Autonoma de Chapingo; México
Fil: Palma Bautista, Candelario. Universidad de Córdoba; España
Fil: Vázquez García, José G.. Universidad de Córdoba; España
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Pampa; Argentina
Fil: Gigón, Ramón. Asesor Privado; Argentina
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal do São Carlos; Brasil
Fil: De Prado, Rafael. Universidad de Córdoba; España
Fil: Portugal, Joao. Instituto Politecnico de Beja; Portugal
description Herbicide-resistant weeds have been identified and recorded on every continent wherecroplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.
publishDate 2023
dc.date.none.fl_str_mv 2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237261
Dominguez Valenzuela, José A.; Palma Bautista, Candelario; Vázquez García, José G.; Yanniccari, Marcos Ezequiel; Gigón, Ramón; et al.; Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America; MDPI; Plants; 12; 11; 5-2023; 1-11
2223-7747
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237261
identifier_str_mv Dominguez Valenzuela, José A.; Palma Bautista, Candelario; Vázquez García, José G.; Yanniccari, Marcos Ezequiel; Gigón, Ramón; et al.; Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America; MDPI; Plants; 12; 11; 5-2023; 1-11
2223-7747
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/plants12112119
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846781881224265728
score 12.982451

Publicaciones similares