D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides

Autores
Palmieri, Valeria Esther; Larran, Alvaro Santiago; Martinatto, Andrea Karina; Permingeat, Hugo Raúl; Perotti, Valeria Elisa
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Amaranthus palmeri S. Watson is a serious problem in soybean crops from Argentina. This weed has evolved high level of resistance to (ALS)-inhibiting herbicides mainly due to a target-site mechanism by an altered ALS enzyme. In an A. palmeri population from Totoras with cross-resistance to (ALS)-inhibiting herbicides, six allelic versions of the ALS enzyme were identified.Objective: The aim of this study was to evaluate plants from that resistant population carrying the ALS substitutions A122S, D376E or A205V, which had not been characterized before for this species.Methods: Subpopulations with each substitution were produced by a vegetative cloning procedure or by cross-pollination and dose-response assays and herbicide single-dose tests were performed to evaluate in vivo resistance levels to (ALS)-inhibiting herbicides.Results: Dose-response experiments showed that all the resistant subpopulations survived at the highest doses tested (32 X) for imidazolinones, triazolopyrimidines and sulfonylureas, while the susceptible population was completely controlled at considerably lower doses. Furthermore, an analysis of the novel A122S substitution showed that it provides cross-resistance to five classes of (ALS)-inhibiting herbicides, excluding the entire ALS herbicide group as an effective control tool in weed populations carrying this substitution.Conclusions: The results indicated that D376E, A205V and A122S substitutions found for the first time in A. palmeri confer cross-resistance to the most used chemical families from herbicide group 2. Interestingly, it was confirmed that the A205V substitution conferred resistance to herbicides in the triazolopyrimidines family. Data generated should be considered in management strategies for delaying the spread of resistance.
Fil: Palmieri, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Larran, Alvaro Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Martinatto, Andrea Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Permingeat, Hugo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Materia
ALS isoforms
Herbicide resistant weeds
Amaranthus palmeri
Propagative cloning
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/216264
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/216264
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicidesPalmieri, Valeria EstherLarran, Alvaro SantiagoMartinatto, Andrea KarinaPermingeat, Hugo RaúlPerotti, Valeria ElisaALS isoformsHerbicide resistant weedsAmaranthus palmeriPropagative cloninghttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Background: Amaranthus palmeri S. Watson is a serious problem in soybean crops from Argentina. This weed has evolved high level of resistance to (ALS)-inhibiting herbicides mainly due to a target-site mechanism by an altered ALS enzyme. In an A. palmeri population from Totoras with cross-resistance to (ALS)-inhibiting herbicides, six allelic versions of the ALS enzyme were identified.Objective: The aim of this study was to evaluate plants from that resistant population carrying the ALS substitutions A122S, D376E or A205V, which had not been characterized before for this species.Methods: Subpopulations with each substitution were produced by a vegetative cloning procedure or by cross-pollination and dose-response assays and herbicide single-dose tests were performed to evaluate in vivo resistance levels to (ALS)-inhibiting herbicides.Results: Dose-response experiments showed that all the resistant subpopulations survived at the highest doses tested (32 X) for imidazolinones, triazolopyrimidines and sulfonylureas, while the susceptible population was completely controlled at considerably lower doses. Furthermore, an analysis of the novel A122S substitution showed that it provides cross-resistance to five classes of (ALS)-inhibiting herbicides, excluding the entire ALS herbicide group as an effective control tool in weed populations carrying this substitution.Conclusions: The results indicated that D376E, A205V and A122S substitutions found for the first time in A. palmeri confer cross-resistance to the most used chemical families from herbicide group 2. Interestingly, it was confirmed that the A205V substitution conferred resistance to herbicides in the triazolopyrimidines family. Data generated should be considered in management strategies for delaying the spread of resistance.Fil: Palmieri, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Larran, Alvaro Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Martinatto, Andrea Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Permingeat, Hugo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaFil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; ArgentinaSociedade Brasileira da Ciência das Plantas Daninhas2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/216264Palmieri, Valeria Esther; Larran, Alvaro Santiago; Martinatto, Andrea Karina; Permingeat, Hugo Raúl; Perotti, Valeria Elisa; D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides; Sociedade Brasileira da Ciência das Plantas Daninhas; Advances in Weed Science; 40; 2; 2-2022; 1-72675-9462CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.scielo.br/j/aws/a/LH7TF5c9gJkrdjkGWMk6zfz/info:eu-repo/semantics/altIdentifier/doi/10.51694/AdvWeedSci/2022;40:Amaranthus004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:01:01Zoai:ri.conicet.gov.ar:11336/216264instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:01:02.274CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides
title D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides
spellingShingle D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides
Palmieri, Valeria Esther
ALS isoforms
Herbicide resistant weeds
Amaranthus palmeri
Propagative cloning
title_short D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides
title_full D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides
title_fullStr D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides
title_full_unstemmed D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides
title_sort D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides
dc.creator.none.fl_str_mv Palmieri, Valeria Esther
Larran, Alvaro Santiago
Martinatto, Andrea Karina
Permingeat, Hugo Raúl
Perotti, Valeria Elisa
author Palmieri, Valeria Esther
author_facet Palmieri, Valeria Esther
Larran, Alvaro Santiago
Martinatto, Andrea Karina
Permingeat, Hugo Raúl
Perotti, Valeria Elisa
author_role author
author2 Larran, Alvaro Santiago
Martinatto, Andrea Karina
Permingeat, Hugo Raúl
Perotti, Valeria Elisa
author2_role author
author
author
author
dc.subject.none.fl_str_mv ALS isoforms
Herbicide resistant weeds
Amaranthus palmeri
Propagative cloning
topic ALS isoforms
Herbicide resistant weeds
Amaranthus palmeri
Propagative cloning
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Background: Amaranthus palmeri S. Watson is a serious problem in soybean crops from Argentina. This weed has evolved high level of resistance to (ALS)-inhibiting herbicides mainly due to a target-site mechanism by an altered ALS enzyme. In an A. palmeri population from Totoras with cross-resistance to (ALS)-inhibiting herbicides, six allelic versions of the ALS enzyme were identified.Objective: The aim of this study was to evaluate plants from that resistant population carrying the ALS substitutions A122S, D376E or A205V, which had not been characterized before for this species.Methods: Subpopulations with each substitution were produced by a vegetative cloning procedure or by cross-pollination and dose-response assays and herbicide single-dose tests were performed to evaluate in vivo resistance levels to (ALS)-inhibiting herbicides.Results: Dose-response experiments showed that all the resistant subpopulations survived at the highest doses tested (32 X) for imidazolinones, triazolopyrimidines and sulfonylureas, while the susceptible population was completely controlled at considerably lower doses. Furthermore, an analysis of the novel A122S substitution showed that it provides cross-resistance to five classes of (ALS)-inhibiting herbicides, excluding the entire ALS herbicide group as an effective control tool in weed populations carrying this substitution.Conclusions: The results indicated that D376E, A205V and A122S substitutions found for the first time in A. palmeri confer cross-resistance to the most used chemical families from herbicide group 2. Interestingly, it was confirmed that the A205V substitution conferred resistance to herbicides in the triazolopyrimidines family. Data generated should be considered in management strategies for delaying the spread of resistance.
Fil: Palmieri, Valeria Esther. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Larran, Alvaro Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Martinatto, Andrea Karina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Permingeat, Hugo Raúl. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina
description Background: Amaranthus palmeri S. Watson is a serious problem in soybean crops from Argentina. This weed has evolved high level of resistance to (ALS)-inhibiting herbicides mainly due to a target-site mechanism by an altered ALS enzyme. In an A. palmeri population from Totoras with cross-resistance to (ALS)-inhibiting herbicides, six allelic versions of the ALS enzyme were identified.Objective: The aim of this study was to evaluate plants from that resistant population carrying the ALS substitutions A122S, D376E or A205V, which had not been characterized before for this species.Methods: Subpopulations with each substitution were produced by a vegetative cloning procedure or by cross-pollination and dose-response assays and herbicide single-dose tests were performed to evaluate in vivo resistance levels to (ALS)-inhibiting herbicides.Results: Dose-response experiments showed that all the resistant subpopulations survived at the highest doses tested (32 X) for imidazolinones, triazolopyrimidines and sulfonylureas, while the susceptible population was completely controlled at considerably lower doses. Furthermore, an analysis of the novel A122S substitution showed that it provides cross-resistance to five classes of (ALS)-inhibiting herbicides, excluding the entire ALS herbicide group as an effective control tool in weed populations carrying this substitution.Conclusions: The results indicated that D376E, A205V and A122S substitutions found for the first time in A. palmeri confer cross-resistance to the most used chemical families from herbicide group 2. Interestingly, it was confirmed that the A205V substitution conferred resistance to herbicides in the triazolopyrimidines family. Data generated should be considered in management strategies for delaying the spread of resistance.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/216264
Palmieri, Valeria Esther; Larran, Alvaro Santiago; Martinatto, Andrea Karina; Permingeat, Hugo Raúl; Perotti, Valeria Elisa; D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides; Sociedade Brasileira da Ciência das Plantas Daninhas; Advances in Weed Science; 40; 2; 2-2022; 1-7
2675-9462
CONICET Digital
CONICET
url http://hdl.handle.net/11336/216264
identifier_str_mv Palmieri, Valeria Esther; Larran, Alvaro Santiago; Martinatto, Andrea Karina; Permingeat, Hugo Raúl; Perotti, Valeria Elisa; D376E, A205V and A122S substitutions recently found in A. palmeri confer cross-resistance to ALS-inhibiting herbicides; Sociedade Brasileira da Ciência das Plantas Daninhas; Advances in Weed Science; 40; 2; 2-2022; 1-7
2675-9462
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/doi/10.51694/AdvWeedSci/2022;40:Amaranthus004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira da Ciência das Plantas Daninhas
publisher.none.fl_str_mv Sociedade Brasileira da Ciência das Plantas Daninhas
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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