Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America

Autores
Dominguez-Valenzuela, José Alfredo; Palma Bautista, Candelario; Vázquez García, José Guadalupe; Yanniccari, Marcos; Gigon, Ramon; Alcántara de la Cruz, Ricardo; de Prado, Rafael; Portugal, João
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS-inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross-herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.
EEA Barrow
Fil: Dominguez-Valenzuela, José Alfredo. Universidad Autónoma de Chapingo. Department of Agricultural Parasitology; México
Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal de São Carlos. Departamento de Química; Brasil
Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Portugal, João. Polytechnic Institute of Beja. Biosciences Department; Portugal
Fil: Portugal, João. Polytechnic Institute of Portalegre. VALORIZA-Research Centre for Endogenous Resource Valorization; Portugal
Fuente
Plants 12 (11) : 2119. (May 2023)
Materia
Auxinas
Resistencia a los Herbicidas
Brassica rapa
Argentina
Mexico
Semilla de Colza
Auxins
Herbicide Resistance
Rapeseed
Acetolactate Synthase
5-enolpyruvylshikimate-3-phosphate
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14636
Acceder
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oai_identifier_str oai:localhost:20.500.12123/14636
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South AmericaDominguez-Valenzuela, José AlfredoPalma Bautista, CandelarioVázquez García, José GuadalupeYanniccari, MarcosGigon, RamonAlcántara de la Cruz, Ricardode Prado, RafaelPortugal, JoãoAuxinasResistencia a los HerbicidasBrassica rapaArgentinaMexicoSemilla de ColzaAuxinsHerbicide ResistanceRapeseedAcetolactate Synthase5-enolpyruvylshikimate-3-phosphateHerbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS-inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross-herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.EEA BarrowFil: Dominguez-Valenzuela, José Alfredo. Universidad Autónoma de Chapingo. Department of Agricultural Parasitology; MéxicoFil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; ArgentinaFil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Gigón, Ramón. Consultor privado en Control de Malezas; ArgentinaFil: Alcántara de la Cruz, Ricardo. Universidade Federal de São Carlos. Departamento de Química; BrasilFil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; EspañaFil: Portugal, João. Polytechnic Institute of Beja. Biosciences Department; PortugalFil: Portugal, João. Polytechnic Institute of Portalegre. VALORIZA-Research Centre for Endogenous Resource Valorization; PortugalMDPI2023-06-16T16:05:41Z2023-06-16T16:05:41Z2023-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14636https://www.mdpi.com/2223-7747/12/11/21192223-7747https://doi.org/10.3390/plants12112119Plants 12 (11) : 2119. (May 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:18:22Zoai:localhost:20.500.12123/14636instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:22.59INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
spellingShingle Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
Dominguez-Valenzuela, José Alfredo
Auxinas
Resistencia a los Herbicidas
Brassica rapa
Argentina
Mexico
Semilla de Colza
Auxins
Herbicide Resistance
Rapeseed
Acetolactate Synthase
5-enolpyruvylshikimate-3-phosphate
title_short Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title_full Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title_fullStr Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title_full_unstemmed Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
title_sort Convergent Adaptation of Multiple Herbicide Resistance to Auxin Mimics and ALS- and EPSPS-Inhibitors in Brassica rapa from North and South America
dc.creator.none.fl_str_mv Dominguez-Valenzuela, José Alfredo
Palma Bautista, Candelario
Vázquez García, José Guadalupe
Yanniccari, Marcos
Gigon, Ramon
Alcántara de la Cruz, Ricardo
de Prado, Rafael
Portugal, João
author Dominguez-Valenzuela, José Alfredo
author_facet Dominguez-Valenzuela, José Alfredo
Palma Bautista, Candelario
Vázquez García, José Guadalupe
Yanniccari, Marcos
Gigon, Ramon
Alcántara de la Cruz, Ricardo
de Prado, Rafael
Portugal, João
author_role author
author2 Palma Bautista, Candelario
Vázquez García, José Guadalupe
Yanniccari, Marcos
Gigon, Ramon
Alcántara de la Cruz, Ricardo
de Prado, Rafael
Portugal, João
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Auxinas
Resistencia a los Herbicidas
Brassica rapa
Argentina
Mexico
Semilla de Colza
Auxins
Herbicide Resistance
Rapeseed
Acetolactate Synthase
5-enolpyruvylshikimate-3-phosphate
topic Auxinas
Resistencia a los Herbicidas
Brassica rapa
Argentina
Mexico
Semilla de Colza
Auxins
Herbicide Resistance
Rapeseed
Acetolactate Synthase
5-enolpyruvylshikimate-3-phosphate
dc.description.none.fl_txt_mv Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS-inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross-herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.
EEA Barrow
Fil: Dominguez-Valenzuela, José Alfredo. Universidad Autónoma de Chapingo. Department of Agricultural Parasitology; México
Fil: Palma Bautista, Candelario. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Vázquez García, José G. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Yanniccari, Marcos Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Chacra Experimental Integrada Barrow; Argentina
Fil: Yanniccari, Marcos Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Yanniccari, Marcos Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Gigón, Ramón. Consultor privado en Control de Malezas; Argentina
Fil: Alcántara de la Cruz, Ricardo. Universidade Federal de São Carlos. Departamento de Química; Brasil
Fil: de Prado, Rafael. Universidad de Córdoba. Department of Agroforestry, Plant Biochemistry and Molecular Biology; España
Fil: Portugal, João. Polytechnic Institute of Beja. Biosciences Department; Portugal
Fil: Portugal, João. Polytechnic Institute of Portalegre. VALORIZA-Research Centre for Endogenous Resource Valorization; Portugal
description Herbicide-resistant weeds have been identified and recorded on every continent where croplands are available. Despite the diversity of weed communities, it is of interest how selection has led to the same consequences in distant regions. Brassica rapa is a widespread naturalized weed that is found throughout temperate North and South America, and it is a frequent weed among winter cereal crops in Argentina and in Mexico. Broadleaf weed control is based on glyphosate that is used prior to sowing and sulfonylureas or mimic auxin herbicides that are used once the weeds have already emerged. This study was aimed at determining whether a convergent phenotypic adaptation to multiple herbicides had occurred in B. rapa populations from Mexico and Argentina by comparing the herbicide sensitivity to inhibitors of the acetolactate synthase (ALS), 5-enolpyruvylshikimate-3-phosphate (EPSPS), and auxin mimics. Five B. rapa populations were analyzed from seeds collected in wheat fields in Argentina (Ar1 and Ar2) and barley fields in Mexico (Mx1, Mx2 and MxS). Mx1, Mx2, and Ar1 populations presented multiple resistance to ALS- and EPSPS-inhibitors and to auxin mimics (2,4-D, MCPA, and fluroxypyr), while the Ar2 population showed resistance only to ALS-inhibitors and glyphosate. Resistance factors ranged from 947 to 4069 for tribenuron-methyl, from 1.5 to 9.4 for 2,4-D, and from 2.7 to 42 for glyphosate. These were consistent with ALS activity, ethylene production, and shikimate accumulation analyses in response to tribenuron-methyl, 2,4-D, and glyphosate, respectively. These results fully support the evolution of the multiple- and cross-herbicide resistance to glyphosate, ALS-inhibitors, and auxinic herbicides in B. rapa populations from Mexico and Argentina.
publishDate 2023
dc.date.none.fl_str_mv 2023-06-16T16:05:41Z
2023-06-16T16:05:41Z
2023-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14636
https://www.mdpi.com/2223-7747/12/11/2119
2223-7747
https://doi.org/10.3390/plants12112119
url http://hdl.handle.net/20.500.12123/14636
https://www.mdpi.com/2223-7747/12/11/2119
https://doi.org/10.3390/plants12112119
identifier_str_mv 2223-7747
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Plants 12 (11) : 2119. (May 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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