Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis

Autores
Price, Edwin R.; Brun, Antonio; Fasulo, Verónica; Karasov, William H.; Caviedes Vidal, Enrique Juan Raul
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Flying vertebrates have been hypothesized to have a high capacity for paracellular absorption of nutrients. This could be due to high permeability of the intestines to nutrient-sized molecules. We performed intestinal luminal perfusions of an insectivorous bat, Tadarida brasiliensis. Using radio-labeled molecules, we measured the uptake of two nutrients absorbed by paracellular and transporter-mediated mechanisms (L-proline, MW 115, and D-glucose, MW180) and two carbohydrates that have no mediated transport (L-arabinose, MW 150, and lactulose, MW 342). Absorption of lactulose (0.61±0.06 nmol min-1 cm-1) was significantly lower than that of the smaller arabinose (1.09±0.04 nmol min-1 cm-1). Glucose absorption was significantly lower than that of proline at both nutrient concentrations (10 mM and 75 mM). Using the absorption of arabinose to estimate the portion of proline absorption that is paracellular, we calculated that 25.1±3.0% to 66.2±7.8% of proline absorption is not transporter mediated (varying proline from 1 mM to 75 mM). These results confirm our predictions that 1) paracellular absorption is molecule size selective, 2) absorption of proline would be greater than glucose absorption in an insectivore, and 3) paracellular absorption represents a large fraction of total nutrient absorption in bats
Fil: Price, Edwin R.. University of Wisconsin. Department of Forest and Wildlife Ecology; Estados Unidos
Fil: Brun, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigación Biológica de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina
Fil: Fasulo, Verónica. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas; Argentina
Fil: Karasov, William H.. University of Wisconsin. Department of Forest and Wildlife Ecology; Estados Unidos
Fil: Caviedes Vidal, Enrique Juan Raul. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Materia
Arabinose
Bats
Flight
Nutrient Absorption
Paracellular Absorption
Intestinal Perfusion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/4356
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/4356
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensisPrice, Edwin R.Brun, AntonioFasulo, VerónicaKarasov, William H.Caviedes Vidal, Enrique Juan RaulArabinoseBatsFlightNutrient AbsorptionParacellular AbsorptionIntestinal Perfusionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Flying vertebrates have been hypothesized to have a high capacity for paracellular absorption of nutrients. This could be due to high permeability of the intestines to nutrient-sized molecules. We performed intestinal luminal perfusions of an insectivorous bat, Tadarida brasiliensis. Using radio-labeled molecules, we measured the uptake of two nutrients absorbed by paracellular and transporter-mediated mechanisms (L-proline, MW 115, and D-glucose, MW180) and two carbohydrates that have no mediated transport (L-arabinose, MW 150, and lactulose, MW 342). Absorption of lactulose (0.61±0.06 nmol min-1 cm-1) was significantly lower than that of the smaller arabinose (1.09±0.04 nmol min-1 cm-1). Glucose absorption was significantly lower than that of proline at both nutrient concentrations (10 mM and 75 mM). Using the absorption of arabinose to estimate the portion of proline absorption that is paracellular, we calculated that 25.1±3.0% to 66.2±7.8% of proline absorption is not transporter mediated (varying proline from 1 mM to 75 mM). These results confirm our predictions that 1) paracellular absorption is molecule size selective, 2) absorption of proline would be greater than glucose absorption in an insectivore, and 3) paracellular absorption represents a large fraction of total nutrient absorption in batsFil: Price, Edwin R.. University of Wisconsin. Department of Forest and Wildlife Ecology; Estados UnidosFil: Brun, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigación Biológica de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; ArgentinaFil: Fasulo, Verónica. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas; ArgentinaFil: Karasov, William H.. University of Wisconsin. Department of Forest and Wildlife Ecology; Estados UnidosFil: Caviedes Vidal, Enrique Juan Raul. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaElsevier2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/4356Price, Edwin R.; Brun, Antonio; Fasulo, Verónica; Karasov, William H.; Caviedes Vidal, Enrique Juan Raul; Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis; Elsevier; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 164; 2; 3-2013; 351-3551095-6433enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1095643312005016info:eu-repo/semantics/altIdentifier/issn/1095-6433info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2012.11.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:08:25Zoai:ri.conicet.gov.ar:11336/4356instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:08:26.171CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis
title Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis
spellingShingle Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis
Price, Edwin R.
Arabinose
Bats
Flight
Nutrient Absorption
Paracellular Absorption
Intestinal Perfusion
title_short Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis
title_full Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis
title_fullStr Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis
title_full_unstemmed Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis
title_sort Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis
dc.creator.none.fl_str_mv Price, Edwin R.
Brun, Antonio
Fasulo, Verónica
Karasov, William H.
Caviedes Vidal, Enrique Juan Raul
author Price, Edwin R.
author_facet Price, Edwin R.
Brun, Antonio
Fasulo, Verónica
Karasov, William H.
Caviedes Vidal, Enrique Juan Raul
author_role author
author2 Brun, Antonio
Fasulo, Verónica
Karasov, William H.
Caviedes Vidal, Enrique Juan Raul
author2_role author
author
author
author
dc.subject.none.fl_str_mv Arabinose
Bats
Flight
Nutrient Absorption
Paracellular Absorption
Intestinal Perfusion
topic Arabinose
Bats
Flight
Nutrient Absorption
Paracellular Absorption
Intestinal Perfusion
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Flying vertebrates have been hypothesized to have a high capacity for paracellular absorption of nutrients. This could be due to high permeability of the intestines to nutrient-sized molecules. We performed intestinal luminal perfusions of an insectivorous bat, Tadarida brasiliensis. Using radio-labeled molecules, we measured the uptake of two nutrients absorbed by paracellular and transporter-mediated mechanisms (L-proline, MW 115, and D-glucose, MW180) and two carbohydrates that have no mediated transport (L-arabinose, MW 150, and lactulose, MW 342). Absorption of lactulose (0.61±0.06 nmol min-1 cm-1) was significantly lower than that of the smaller arabinose (1.09±0.04 nmol min-1 cm-1). Glucose absorption was significantly lower than that of proline at both nutrient concentrations (10 mM and 75 mM). Using the absorption of arabinose to estimate the portion of proline absorption that is paracellular, we calculated that 25.1±3.0% to 66.2±7.8% of proline absorption is not transporter mediated (varying proline from 1 mM to 75 mM). These results confirm our predictions that 1) paracellular absorption is molecule size selective, 2) absorption of proline would be greater than glucose absorption in an insectivore, and 3) paracellular absorption represents a large fraction of total nutrient absorption in bats
Fil: Price, Edwin R.. University of Wisconsin. Department of Forest and Wildlife Ecology; Estados Unidos
Fil: Brun, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigación Biológica de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina
Fil: Fasulo, Verónica. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. Universidad Nacional de San Luis. Facultad de Ciencias Humanas; Argentina
Fil: Karasov, William H.. University of Wisconsin. Department of Forest and Wildlife Ecology; Estados Unidos
Fil: Caviedes Vidal, Enrique Juan Raul. Universidad Nacional de San Luis. Facultad de Ciencias Humanas. Laboratorio de Biología "Profesor Enrique Cavides Codelia"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
description Flying vertebrates have been hypothesized to have a high capacity for paracellular absorption of nutrients. This could be due to high permeability of the intestines to nutrient-sized molecules. We performed intestinal luminal perfusions of an insectivorous bat, Tadarida brasiliensis. Using radio-labeled molecules, we measured the uptake of two nutrients absorbed by paracellular and transporter-mediated mechanisms (L-proline, MW 115, and D-glucose, MW180) and two carbohydrates that have no mediated transport (L-arabinose, MW 150, and lactulose, MW 342). Absorption of lactulose (0.61±0.06 nmol min-1 cm-1) was significantly lower than that of the smaller arabinose (1.09±0.04 nmol min-1 cm-1). Glucose absorption was significantly lower than that of proline at both nutrient concentrations (10 mM and 75 mM). Using the absorption of arabinose to estimate the portion of proline absorption that is paracellular, we calculated that 25.1±3.0% to 66.2±7.8% of proline absorption is not transporter mediated (varying proline from 1 mM to 75 mM). These results confirm our predictions that 1) paracellular absorption is molecule size selective, 2) absorption of proline would be greater than glucose absorption in an insectivore, and 3) paracellular absorption represents a large fraction of total nutrient absorption in bats
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/4356
Price, Edwin R.; Brun, Antonio; Fasulo, Verónica; Karasov, William H.; Caviedes Vidal, Enrique Juan Raul; Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis; Elsevier; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 164; 2; 3-2013; 351-355
1095-6433
url http://hdl.handle.net/11336/4356
identifier_str_mv Price, Edwin R.; Brun, Antonio; Fasulo, Verónica; Karasov, William H.; Caviedes Vidal, Enrique Juan Raul; Intestinal perfusion indicates high reliance on paracellular nutrient absorption in an insectivorous bat Tadarida brasiliensis; Elsevier; Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology; 164; 2; 3-2013; 351-355
1095-6433
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S1095643312005016
info:eu-repo/semantics/altIdentifier/issn/1095-6433
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpa.2012.11.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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