High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments

Autores
Brun, Antonio; Price, Edwin R.; Gontero Fourcade, Manuel Nicolas; Fernández Marinone, Guido; Cruz Neto, Ariovaldo P.; Caviedes Vidal, Enrique Juan Raul; Karasov, William H.
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Water-soluble nutrients are absorbed by the small intestine via transcellular and paracellular mechanisms. Based on a few previous studies, the capacity for paracellular nutrient absorption seems greater in flying mammals than in nonflying mammals, but there has been little investigation of the mechanisms driving this difference. Therefore, we studied three species each of bats (Artibeus lituratus, Sturnira lilium and Carollia perspicillata) and nonflying mammals (Akodon montensis, Mus musculus and Rattus norvegicus). Using standard pharmacokinetic techniques in intact animals, we confirmed the greater paracellular nutrient absorption in the fliers, comparing one species in each group. Then we conducted in situ intestinal perfusions on individuals of all species. In both approaches, we measured the absorption of 3OMD-glucose, a nonmetabolizable glucose analog absorbed both paracellularly and transcellularly, as well as l-arabinose, which has no mediated transport. Fractional absorption of l-arabinose was three times higher in the bat (S. lilium: 1.2±0.24) than in the rodent (A. montensis: 0.35±0.04), whereas fractional absorption of 3OMD-glucose was complete in both species (1.46±0.4 and 0.97±0.12, respectively). In agreement, bats exhibited two to 12 times higher l-arabinose clearance per square centimeter nominal surface area than rodents in intestinal perfusions. Using l-arabinose, we estimated that the contribution of the paracellular pathway to total glucose absorption was higher in all three bats (109–137%) than in the rodents (13–39%). These findings contribute to an emerging picture that reliance on the paracellular pathway for nutrient absorption is much greater in bats relative to nonflying mammals and that this difference is driven by differences in intestinal permeability to nutrient-sized molecules.
Fil: Brun, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Price, Edwin R.. University Of Wisconsin; Estados Unidos
Fil: Gontero Fourcade, Manuel Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Fernández Marinone, Guido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Cruz Neto, Ariovaldo P.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Caviedes Vidal, Enrique Juan Raul. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Karasov, William H.. University of Wisconsin; Estados Unidos
Materia
Intestine
Mediated absorption
Paracellular absorption
Arabinose
Bats
Flight
Intestinal perfusion
Akodon montensis
Sturnira lilium
Artibeus lituratus
Carollia perspicillata
Mus musculus
Rattus norvegicus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/14686
Acceder
id CONICETDig_7ab4cb62b148fc58ae3fada4e2380e40
oai_identifier_str oai:ri.conicet.gov.ar:11336/14686
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segmentsBrun, AntonioPrice, Edwin R.Gontero Fourcade, Manuel NicolasFernández Marinone, GuidoCruz Neto, Ariovaldo P.Caviedes Vidal, Enrique Juan RaulKarasov, William H.IntestineMediated absorptionParacellular absorptionArabinoseBatsFlightIntestinal perfusionAkodon montensisSturnira liliumArtibeus lituratusCarollia perspicillataMus musculusRattus norvegicushttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Water-soluble nutrients are absorbed by the small intestine via transcellular and paracellular mechanisms. Based on a few previous studies, the capacity for paracellular nutrient absorption seems greater in flying mammals than in nonflying mammals, but there has been little investigation of the mechanisms driving this difference. Therefore, we studied three species each of bats (Artibeus lituratus, Sturnira lilium and Carollia perspicillata) and nonflying mammals (Akodon montensis, Mus musculus and Rattus norvegicus). Using standard pharmacokinetic techniques in intact animals, we confirmed the greater paracellular nutrient absorption in the fliers, comparing one species in each group. Then we conducted in situ intestinal perfusions on individuals of all species. In both approaches, we measured the absorption of 3OMD-glucose, a nonmetabolizable glucose analog absorbed both paracellularly and transcellularly, as well as l-arabinose, which has no mediated transport. Fractional absorption of l-arabinose was three times higher in the bat (S. lilium: 1.2±0.24) than in the rodent (A. montensis: 0.35±0.04), whereas fractional absorption of 3OMD-glucose was complete in both species (1.46±0.4 and 0.97±0.12, respectively). In agreement, bats exhibited two to 12 times higher l-arabinose clearance per square centimeter nominal surface area than rodents in intestinal perfusions. Using l-arabinose, we estimated that the contribution of the paracellular pathway to total glucose absorption was higher in all three bats (109–137%) than in the rodents (13–39%). These findings contribute to an emerging picture that reliance on the paracellular pathway for nutrient absorption is much greater in bats relative to nonflying mammals and that this difference is driven by differences in intestinal permeability to nutrient-sized molecules.Fil: Brun, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Price, Edwin R.. University Of Wisconsin; Estados UnidosFil: Gontero Fourcade, Manuel Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Fernández Marinone, Guido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; ArgentinaFil: Cruz Neto, Ariovaldo P.. Universidade Estadual Paulista Julio de Mesquita Filho; BrasilFil: Caviedes Vidal, Enrique Juan Raul. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; ArgentinaFil: Karasov, William H.. University of Wisconsin; Estados UnidosCompany of Biologists2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/14686Brun, Antonio; Price, Edwin R.; Gontero Fourcade, Manuel Nicolas; Fernández Marinone, Guido; Cruz Neto, Ariovaldo P.; et al.; High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments; Company of Biologists; Journal of Experimental Biology; 217; 18; 7-2014; 3311-33170022-09491477-9145enginfo:eu-repo/semantics/altIdentifier/url/http://jeb.biologists.org/content/217/18/3311info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.104927info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:24:47Zoai:ri.conicet.gov.ar:11336/14686instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:24:47.359CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments
title High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments
spellingShingle High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments
Brun, Antonio
Intestine
Mediated absorption
Paracellular absorption
Arabinose
Bats
Flight
Intestinal perfusion
Akodon montensis
Sturnira lilium
Artibeus lituratus
Carollia perspicillata
Mus musculus
Rattus norvegicus
title_short High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments
title_full High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments
title_fullStr High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments
title_full_unstemmed High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments
title_sort High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments
dc.creator.none.fl_str_mv Brun, Antonio
Price, Edwin R.
Gontero Fourcade, Manuel Nicolas
Fernández Marinone, Guido
Cruz Neto, Ariovaldo P.
Caviedes Vidal, Enrique Juan Raul
Karasov, William H.
author Brun, Antonio
author_facet Brun, Antonio
Price, Edwin R.
Gontero Fourcade, Manuel Nicolas
Fernández Marinone, Guido
Cruz Neto, Ariovaldo P.
Caviedes Vidal, Enrique Juan Raul
Karasov, William H.
author_role author
author2 Price, Edwin R.
Gontero Fourcade, Manuel Nicolas
Fernández Marinone, Guido
Cruz Neto, Ariovaldo P.
Caviedes Vidal, Enrique Juan Raul
Karasov, William H.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Intestine
Mediated absorption
Paracellular absorption
Arabinose
Bats
Flight
Intestinal perfusion
Akodon montensis
Sturnira lilium
Artibeus lituratus
Carollia perspicillata
Mus musculus
Rattus norvegicus
topic Intestine
Mediated absorption
Paracellular absorption
Arabinose
Bats
Flight
Intestinal perfusion
Akodon montensis
Sturnira lilium
Artibeus lituratus
Carollia perspicillata
Mus musculus
Rattus norvegicus
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Water-soluble nutrients are absorbed by the small intestine via transcellular and paracellular mechanisms. Based on a few previous studies, the capacity for paracellular nutrient absorption seems greater in flying mammals than in nonflying mammals, but there has been little investigation of the mechanisms driving this difference. Therefore, we studied three species each of bats (Artibeus lituratus, Sturnira lilium and Carollia perspicillata) and nonflying mammals (Akodon montensis, Mus musculus and Rattus norvegicus). Using standard pharmacokinetic techniques in intact animals, we confirmed the greater paracellular nutrient absorption in the fliers, comparing one species in each group. Then we conducted in situ intestinal perfusions on individuals of all species. In both approaches, we measured the absorption of 3OMD-glucose, a nonmetabolizable glucose analog absorbed both paracellularly and transcellularly, as well as l-arabinose, which has no mediated transport. Fractional absorption of l-arabinose was three times higher in the bat (S. lilium: 1.2±0.24) than in the rodent (A. montensis: 0.35±0.04), whereas fractional absorption of 3OMD-glucose was complete in both species (1.46±0.4 and 0.97±0.12, respectively). In agreement, bats exhibited two to 12 times higher l-arabinose clearance per square centimeter nominal surface area than rodents in intestinal perfusions. Using l-arabinose, we estimated that the contribution of the paracellular pathway to total glucose absorption was higher in all three bats (109–137%) than in the rodents (13–39%). These findings contribute to an emerging picture that reliance on the paracellular pathway for nutrient absorption is much greater in bats relative to nonflying mammals and that this difference is driven by differences in intestinal permeability to nutrient-sized molecules.
Fil: Brun, Antonio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Price, Edwin R.. University Of Wisconsin; Estados Unidos
Fil: Gontero Fourcade, Manuel Nicolas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Fernández Marinone, Guido. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina
Fil: Cruz Neto, Ariovaldo P.. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil
Fil: Caviedes Vidal, Enrique Juan Raul. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto Multidisciplinario de Investigaciones Biológicas de San Luis; Argentina
Fil: Karasov, William H.. University of Wisconsin; Estados Unidos
description Water-soluble nutrients are absorbed by the small intestine via transcellular and paracellular mechanisms. Based on a few previous studies, the capacity for paracellular nutrient absorption seems greater in flying mammals than in nonflying mammals, but there has been little investigation of the mechanisms driving this difference. Therefore, we studied three species each of bats (Artibeus lituratus, Sturnira lilium and Carollia perspicillata) and nonflying mammals (Akodon montensis, Mus musculus and Rattus norvegicus). Using standard pharmacokinetic techniques in intact animals, we confirmed the greater paracellular nutrient absorption in the fliers, comparing one species in each group. Then we conducted in situ intestinal perfusions on individuals of all species. In both approaches, we measured the absorption of 3OMD-glucose, a nonmetabolizable glucose analog absorbed both paracellularly and transcellularly, as well as l-arabinose, which has no mediated transport. Fractional absorption of l-arabinose was three times higher in the bat (S. lilium: 1.2±0.24) than in the rodent (A. montensis: 0.35±0.04), whereas fractional absorption of 3OMD-glucose was complete in both species (1.46±0.4 and 0.97±0.12, respectively). In agreement, bats exhibited two to 12 times higher l-arabinose clearance per square centimeter nominal surface area than rodents in intestinal perfusions. Using l-arabinose, we estimated that the contribution of the paracellular pathway to total glucose absorption was higher in all three bats (109–137%) than in the rodents (13–39%). These findings contribute to an emerging picture that reliance on the paracellular pathway for nutrient absorption is much greater in bats relative to nonflying mammals and that this difference is driven by differences in intestinal permeability to nutrient-sized molecules.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/14686
Brun, Antonio; Price, Edwin R.; Gontero Fourcade, Manuel Nicolas; Fernández Marinone, Guido; Cruz Neto, Ariovaldo P.; et al.; High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments; Company of Biologists; Journal of Experimental Biology; 217; 18; 7-2014; 3311-3317
0022-0949
1477-9145
url http://hdl.handle.net/11336/14686
identifier_str_mv Brun, Antonio; Price, Edwin R.; Gontero Fourcade, Manuel Nicolas; Fernández Marinone, Guido; Cruz Neto, Ariovaldo P.; et al.; High paracellular nutrient absorption in intact bats is associated with high paracellular permeability in perfused intestinal segments; Company of Biologists; Journal of Experimental Biology; 217; 18; 7-2014; 3311-3317
0022-0949
1477-9145
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://jeb.biologists.org/content/217/18/3311
info:eu-repo/semantics/altIdentifier/doi/10.1242/jeb.104927
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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