Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization

Autores
Cohen, Debora Juana; Maldera, Julieta Antonella; Weigel Muñoz, Mariana; Ernesto, Juan Ignacio; Vasen, Gustavo; Cuasnicu, Patricia Sara
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Epididymal protein CRISP1 is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Recent results showed that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, recent results revealed that CRISP1 would also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mice line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2 as well as the comparison of their corresponding S2 regions suggest that both proteins would bind to common complementary sites on the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Maldera, Julieta Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Materia
SPERM
GAMETE FUSION
CRISP
FERTILIZATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10385

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network_name_str CONICET Digital (CONICET)
spelling Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilizationCohen, Debora JuanaMaldera, Julieta AntonellaWeigel Muñoz, MarianaErnesto, Juan IgnacioVasen, GustavoCuasnicu, Patricia SaraSPERMGAMETE FUSIONCRISPFERTILIZATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Epididymal protein CRISP1 is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Recent results showed that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, recent results revealed that CRISP1 would also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mice line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2 as well as the comparison of their corresponding S2 regions suggest that both proteins would bind to common complementary sites on the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Maldera, Julieta Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaSociedad de Biología de Chile2011-09-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10385Cohen, Debora Juana; Maldera, Julieta Antonella; Weigel Muñoz, Mariana; Ernesto, Juan Ignacio; Vasen, Gustavo; et al.; Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization; Sociedad de Biología de Chile; Biological Research; 44; 2; 20-9-2011; 135-1380716-97600717-6287enginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.4067/S0716-97602011000200004info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9b42pninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:44Zoai:ri.conicet.gov.ar:11336/10385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:45.165CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
spellingShingle Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
Cohen, Debora Juana
SPERM
GAMETE FUSION
CRISP
FERTILIZATION
title_short Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_full Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_fullStr Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_full_unstemmed Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
title_sort Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
dc.creator.none.fl_str_mv Cohen, Debora Juana
Maldera, Julieta Antonella
Weigel Muñoz, Mariana
Ernesto, Juan Ignacio
Vasen, Gustavo
Cuasnicu, Patricia Sara
author Cohen, Debora Juana
author_facet Cohen, Debora Juana
Maldera, Julieta Antonella
Weigel Muñoz, Mariana
Ernesto, Juan Ignacio
Vasen, Gustavo
Cuasnicu, Patricia Sara
author_role author
author2 Maldera, Julieta Antonella
Weigel Muñoz, Mariana
Ernesto, Juan Ignacio
Vasen, Gustavo
Cuasnicu, Patricia Sara
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv SPERM
GAMETE FUSION
CRISP
FERTILIZATION
topic SPERM
GAMETE FUSION
CRISP
FERTILIZATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Epididymal protein CRISP1 is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Recent results showed that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, recent results revealed that CRISP1 would also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mice line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2 as well as the comparison of their corresponding S2 regions suggest that both proteins would bind to common complementary sites on the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Maldera, Julieta Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
description Epididymal protein CRISP1 is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Recent results showed that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, recent results revealed that CRISP1 would also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mice line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2 as well as the comparison of their corresponding S2 regions suggest that both proteins would bind to common complementary sites on the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.
publishDate 2011
dc.date.none.fl_str_mv 2011-09-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10385
Cohen, Debora Juana; Maldera, Julieta Antonella; Weigel Muñoz, Mariana; Ernesto, Juan Ignacio; Vasen, Gustavo; et al.; Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization; Sociedad de Biología de Chile; Biological Research; 44; 2; 20-9-2011; 135-138
0716-9760
0717-6287
url http://hdl.handle.net/11336/10385
identifier_str_mv Cohen, Debora Juana; Maldera, Julieta Antonella; Weigel Muñoz, Mariana; Ernesto, Juan Ignacio; Vasen, Gustavo; et al.; Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization; Sociedad de Biología de Chile; Biological Research; 44; 2; 20-9-2011; 135-138
0716-9760
0717-6287
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.4067/S0716-97602011000200004
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9b42pn
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Sociedad de Biología de Chile
publisher.none.fl_str_mv Sociedad de Biología de Chile
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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