Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization
- Autores
- Cohen, Debora Juana; Maldera, Julieta Antonella; Weigel Muñoz, Mariana; Ernesto, Juan Ignacio; Vasen, Gustavo; Cuasnicu, Patricia Sara
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Epididymal protein CRISP1 is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Recent results showed that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, recent results revealed that CRISP1 would also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mice line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2 as well as the comparison of their corresponding S2 regions suggest that both proteins would bind to common complementary sites on the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.
Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Maldera, Julieta Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina
Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina - Materia
-
SPERM
GAMETE FUSION
CRISP
FERTILIZATION - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/10385
Ver los metadatos del registro completo
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Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilizationCohen, Debora JuanaMaldera, Julieta AntonellaWeigel Muñoz, MarianaErnesto, Juan IgnacioVasen, GustavoCuasnicu, Patricia SaraSPERMGAMETE FUSIONCRISPFERTILIZATIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Epididymal protein CRISP1 is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Recent results showed that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, recent results revealed that CRISP1 would also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mice line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2 as well as the comparison of their corresponding S2 regions suggest that both proteins would bind to common complementary sites on the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization.Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Maldera, Julieta Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaFil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); ArgentinaSociedad de Biología de Chile2011-09-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10385Cohen, Debora Juana; Maldera, Julieta Antonella; Weigel Muñoz, Mariana; Ernesto, Juan Ignacio; Vasen, Gustavo; et al.; Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization; Sociedad de Biología de Chile; Biological Research; 44; 2; 20-9-2011; 135-1380716-97600717-6287enginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.4067/S0716-97602011000200004info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9b42pninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:57:44Zoai:ri.conicet.gov.ar:11336/10385instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:57:45.165CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization |
title |
Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization |
spellingShingle |
Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization Cohen, Debora Juana SPERM GAMETE FUSION CRISP FERTILIZATION |
title_short |
Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization |
title_full |
Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization |
title_fullStr |
Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization |
title_full_unstemmed |
Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization |
title_sort |
Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization |
dc.creator.none.fl_str_mv |
Cohen, Debora Juana Maldera, Julieta Antonella Weigel Muñoz, Mariana Ernesto, Juan Ignacio Vasen, Gustavo Cuasnicu, Patricia Sara |
author |
Cohen, Debora Juana |
author_facet |
Cohen, Debora Juana Maldera, Julieta Antonella Weigel Muñoz, Mariana Ernesto, Juan Ignacio Vasen, Gustavo Cuasnicu, Patricia Sara |
author_role |
author |
author2 |
Maldera, Julieta Antonella Weigel Muñoz, Mariana Ernesto, Juan Ignacio Vasen, Gustavo Cuasnicu, Patricia Sara |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
SPERM GAMETE FUSION CRISP FERTILIZATION |
topic |
SPERM GAMETE FUSION CRISP FERTILIZATION |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/3.1 https://purl.org/becyt/ford/3 |
dc.description.none.fl_txt_mv |
Epididymal protein CRISP1 is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Recent results showed that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, recent results revealed that CRISP1 would also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mice line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2 as well as the comparison of their corresponding S2 regions suggest that both proteins would bind to common complementary sites on the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization. Fil: Cohen, Debora Juana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Maldera, Julieta Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Weigel Muñoz, Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Ernesto, Juan Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Vasen, Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina Fil: Cuasnicu, Patricia Sara. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental (i); Argentina |
description |
Epididymal protein CRISP1 is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Recent results showed that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, recent results revealed that CRISP1 would also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mice line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice exhibited normal fertility, CRISP1-defficient sperm presented a decreased level of protein tyrosine phosphorylation during capacitation, and an impaired ability to fertilize both zona-intact and zona-free eggs in vitro, confirming the proposed roles for the protein in fertilization. Evidence obtained in our laboratory indicated that testicular CRISP2 would also be involved in sperm-egg fusion. Competition assays between CRISP1 and CRISP2 as well as the comparison of their corresponding S2 regions suggest that both proteins would bind to common complementary sites on the egg. Together, these results suggest a functional cooperation between CRISP1 and CRISP2 to ensure the success of fertilization. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-20 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/10385 Cohen, Debora Juana; Maldera, Julieta Antonella; Weigel Muñoz, Mariana; Ernesto, Juan Ignacio; Vasen, Gustavo; et al.; Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization; Sociedad de Biología de Chile; Biological Research; 44; 2; 20-9-2011; 135-138 0716-9760 0717-6287 |
url |
http://hdl.handle.net/11336/10385 |
identifier_str_mv |
Cohen, Debora Juana; Maldera, Julieta Antonella; Weigel Muñoz, Mariana; Ernesto, Juan Ignacio; Vasen, Gustavo; et al.; Cysteine-Rich Secretory Proteins (CRISP) and their role in mammalian fertilization; Sociedad de Biología de Chile; Biological Research; 44; 2; 20-9-2011; 135-138 0716-9760 0717-6287 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.4067/S0716-97602011000200004 info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9b42pn |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Sociedad de Biología de Chile |
publisher.none.fl_str_mv |
Sociedad de Biología de Chile |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1842269481473146880 |
score |
13.13397 |