An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests

Autores
Cecchini, Nicolas Miguel; Song, Yi; Roychoudhry, Suruchi; Greenberg, Jean T.; Haney, Cara H.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas spp. with the reference plant Arabidopsis thaliana. Here, we describe a robust, increased throughput, bioassay to study ISR against the bacterial pathogen Pseudomonas cannabina pv. alisalensis (formerly called Pseudomonas syringae pv. maculicola) strain ES4326 and the herbivore Trichoplusia ni by inoculating Pseudomonas simiae strain WCS417 (formerly called Pseudomonas fluorescens WCS417) on Arabidopsis plants grown in Jiffy-7® peat pellets. While most commonly used for Pseudomonas-triggered ISR on Arabidopsis, this assay is effective for diverse rhizosphere bacterial strains, plant species, pathogens and herbivores.
Fil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Song, Yi. University of British Columbia; Canadá
Fil: Roychoudhry, Suruchi. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados Unidos
Fil: Greenberg, Jean T.. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados Unidos
Fil: Haney, Cara H.. University of British Columbia; Canadá
Materia
INDUCED SYSTEMIC RESISTANCE
PSEUDOMONAS SIMIAE
ARABIDOPSIS THALIANA
PSEUDOMONAS SYRINGAE
PSEUDOMONAS CANNABINA
TRICHOPLUSIA NI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/129166
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/129166
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pestsCecchini, Nicolas MiguelSong, YiRoychoudhry, SuruchiGreenberg, Jean T.Haney, Cara H.INDUCED SYSTEMIC RESISTANCEPSEUDOMONAS SIMIAEARABIDOPSIS THALIANAPSEUDOMONAS SYRINGAEPSEUDOMONAS CANNABINATRICHOPLUSIA NIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas spp. with the reference plant Arabidopsis thaliana. Here, we describe a robust, increased throughput, bioassay to study ISR against the bacterial pathogen Pseudomonas cannabina pv. alisalensis (formerly called Pseudomonas syringae pv. maculicola) strain ES4326 and the herbivore Trichoplusia ni by inoculating Pseudomonas simiae strain WCS417 (formerly called Pseudomonas fluorescens WCS417) on Arabidopsis plants grown in Jiffy-7® peat pellets. While most commonly used for Pseudomonas-triggered ISR on Arabidopsis, this assay is effective for diverse rhizosphere bacterial strains, plant species, pathogens and herbivores.Fil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Song, Yi. University of British Columbia; CanadáFil: Roychoudhry, Suruchi. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados UnidosFil: Greenberg, Jean T.. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados UnidosFil: Haney, Cara H.. University of British Columbia; CanadáBio-protocol2019-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/129166Cecchini, Nicolas Miguel; Song, Yi; Roychoudhry, Suruchi; Greenberg, Jean T.; Haney, Cara H.; An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests; Bio-protocol; Bio-protocol; 9; 10; 5-2019; 1-132331-8325CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e3236info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.3236info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-29T12:10:10Zoai:ri.conicet.gov.ar:11336/129166instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-29 12:10:10.444CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests
title An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests
spellingShingle An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests
Cecchini, Nicolas Miguel
INDUCED SYSTEMIC RESISTANCE
PSEUDOMONAS SIMIAE
ARABIDOPSIS THALIANA
PSEUDOMONAS SYRINGAE
PSEUDOMONAS CANNABINA
TRICHOPLUSIA NI
title_short An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests
title_full An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests
title_fullStr An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests
title_full_unstemmed An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests
title_sort An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests
dc.creator.none.fl_str_mv Cecchini, Nicolas Miguel
Song, Yi
Roychoudhry, Suruchi
Greenberg, Jean T.
Haney, Cara H.
author Cecchini, Nicolas Miguel
author_facet Cecchini, Nicolas Miguel
Song, Yi
Roychoudhry, Suruchi
Greenberg, Jean T.
Haney, Cara H.
author_role author
author2 Song, Yi
Roychoudhry, Suruchi
Greenberg, Jean T.
Haney, Cara H.
author2_role author
author
author
author
dc.subject.none.fl_str_mv INDUCED SYSTEMIC RESISTANCE
PSEUDOMONAS SIMIAE
ARABIDOPSIS THALIANA
PSEUDOMONAS SYRINGAE
PSEUDOMONAS CANNABINA
TRICHOPLUSIA NI
topic INDUCED SYSTEMIC RESISTANCE
PSEUDOMONAS SIMIAE
ARABIDOPSIS THALIANA
PSEUDOMONAS SYRINGAE
PSEUDOMONAS CANNABINA
TRICHOPLUSIA NI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas spp. with the reference plant Arabidopsis thaliana. Here, we describe a robust, increased throughput, bioassay to study ISR against the bacterial pathogen Pseudomonas cannabina pv. alisalensis (formerly called Pseudomonas syringae pv. maculicola) strain ES4326 and the herbivore Trichoplusia ni by inoculating Pseudomonas simiae strain WCS417 (formerly called Pseudomonas fluorescens WCS417) on Arabidopsis plants grown in Jiffy-7® peat pellets. While most commonly used for Pseudomonas-triggered ISR on Arabidopsis, this assay is effective for diverse rhizosphere bacterial strains, plant species, pathogens and herbivores.
Fil: Cecchini, Nicolas Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentina
Fil: Song, Yi. University of British Columbia; Canadá
Fil: Roychoudhry, Suruchi. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados Unidos
Fil: Greenberg, Jean T.. University of Chicago. Department of Molecular Genetics and Cell Biology; Estados Unidos
Fil: Haney, Cara H.. University of British Columbia; Canadá
description The plant immune system is essential for plants to perceive and defend against bacterial, fungal and insect pests and pathogens. Induced systemic resistance (ISR) is a systemic immune response that occurs upon root colonization by beneficial microbes. A well-studied model for ISR is the association of specific beneficial strains of Pseudomonas spp. with the reference plant Arabidopsis thaliana. Here, we describe a robust, increased throughput, bioassay to study ISR against the bacterial pathogen Pseudomonas cannabina pv. alisalensis (formerly called Pseudomonas syringae pv. maculicola) strain ES4326 and the herbivore Trichoplusia ni by inoculating Pseudomonas simiae strain WCS417 (formerly called Pseudomonas fluorescens WCS417) on Arabidopsis plants grown in Jiffy-7® peat pellets. While most commonly used for Pseudomonas-triggered ISR on Arabidopsis, this assay is effective for diverse rhizosphere bacterial strains, plant species, pathogens and herbivores.
publishDate 2019
dc.date.none.fl_str_mv 2019-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/129166
Cecchini, Nicolas Miguel; Song, Yi; Roychoudhry, Suruchi; Greenberg, Jean T.; Haney, Cara H.; An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests; Bio-protocol; Bio-protocol; 9; 10; 5-2019; 1-13
2331-8325
CONICET Digital
CONICET
url http://hdl.handle.net/11336/129166
identifier_str_mv Cecchini, Nicolas Miguel; Song, Yi; Roychoudhry, Suruchi; Greenberg, Jean T.; Haney, Cara H.; An improved bioassay to study arabidopsis induced systemic resistance (ISR) against bacterial pathogens and insect pests; Bio-protocol; Bio-protocol; 9; 10; 5-2019; 1-13
2331-8325
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://bio-protocol.org/e3236
info:eu-repo/semantics/altIdentifier/doi/10.21769/BioProtoc.3236
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bio-protocol
publisher.none.fl_str_mv Bio-protocol
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1847426928199335936
score 13.10058

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