LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves

Autores
Moriconi, Victoria; Sellaro, Romina Vanesa; Ayub, Nicolás Daniel; Soto, Gabriela; Rugnone, Matias Leandro; Shah, Rashmi; Pathak, Gopal P.; Gärtner, Wolfgang; Casal, Jorge José
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component system (Pst–Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) modulates virulence against A. thaliana. Bioinformatic analysis and the existence of an episomal circular intermediate indicate that the locus encoding Pst–Lov is present in an active genomic island acquired by horizontal transfer. Strains mutated at Pst–Lov showed enhanced growth on minimal medium and in leaves of A. thaliana exposed to light, but not in leaves incubated in darkness or buried in the soil. Pst–Lov repressed the expression of principal and alternative sigma factor genes and their downstream targets linked to bacterial growth, virulence and quorum sensing, in a strictly light‐dependent manner. We propose that the function of Pst–Lov is to distinguish between soil (dark) and leaf (light) environments, attenuating the damage caused to host tissues while releasing growth out of the host. Therefore, in addition to its direct actions via photosynthesis and plant sensory receptors, light may affect plants indirectly via the sensory receptors of bacterial pathogens.
Instituto de Genética
Fil: Moriconi, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina
Fil: Sellaro, Romina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Rugnone, Matias Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina
Fil: Shah, Rashmi. Max Planck Institute for Chemical Energy Conversion; Alemania
Fil: Pathak, Gopal P.. Max Planck Institute for Chemical Energy Conversion; Alemania
Fil: Gärtner, Wolfgang. Max Planck Institute for Chemical Energy Conversion; Alemania
Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina
Fuente
Plant Journal 76 (2) : 322-331 (October 2013)
Materia
Arabidopsis Thaliana
Pseudomonas syringae
Tomate
Regimenes de Luz
Bioinformática
Tomatoes
Light Regimes
Bioinformatics
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5128
Acceder
id INTADig_fb94bfe1b5766f926ac79033691ec371
oai_identifier_str oai:localhost:20.500.12123/5128
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leavesMoriconi, VictoriaSellaro, Romina VanesaAyub, Nicolás DanielSoto, GabrielaRugnone, Matias LeandroShah, RashmiPathak, Gopal P.Gärtner, WolfgangCasal, Jorge JoséArabidopsis ThalianaPseudomonas syringaeTomateRegimenes de LuzBioinformáticaTomatoesLight RegimesBioinformaticsIn Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component system (Pst–Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) modulates virulence against A. thaliana. Bioinformatic analysis and the existence of an episomal circular intermediate indicate that the locus encoding Pst–Lov is present in an active genomic island acquired by horizontal transfer. Strains mutated at Pst–Lov showed enhanced growth on minimal medium and in leaves of A. thaliana exposed to light, but not in leaves incubated in darkness or buried in the soil. Pst–Lov repressed the expression of principal and alternative sigma factor genes and their downstream targets linked to bacterial growth, virulence and quorum sensing, in a strictly light‐dependent manner. We propose that the function of Pst–Lov is to distinguish between soil (dark) and leaf (light) environments, attenuating the damage caused to host tissues while releasing growth out of the host. Therefore, in addition to its direct actions via photosynthesis and plant sensory receptors, light may affect plants indirectly via the sensory receptors of bacterial pathogens.Instituto de GenéticaFil: Moriconi, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; ArgentinaFil: Sellaro, Romina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.Fil: Rugnone, Matias Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; ArgentinaFil: Shah, Rashmi. Max Planck Institute for Chemical Energy Conversion; AlemaniaFil: Pathak, Gopal P.. Max Planck Institute for Chemical Energy Conversion; AlemaniaFil: Gärtner, Wolfgang. Max Planck Institute for Chemical Energy Conversion; AlemaniaFil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; ArgentinaWiley2019-05-16T12:51:23Z2019-05-16T12:51:23Z2013-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://onlinelibrary.wiley.com/doi/10.1111/tpj.12289/abstracthttp://hdl.handle.net/20.500.12123/51280960-74121365-313Xhttps://doi.org/10.1111/tpj.12289Plant Journal 76 (2) : 322-331 (October 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:57Zoai:localhost:20.500.12123/5128instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:58.474INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
title LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
spellingShingle LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
Moriconi, Victoria
Arabidopsis Thaliana
Pseudomonas syringae
Tomate
Regimenes de Luz
Bioinformática
Tomatoes
Light Regimes
Bioinformatics
title_short LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
title_full LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
title_fullStr LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
title_full_unstemmed LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
title_sort LOV‐domain photoreceptor, encoded in a genomic island, attenuates the virulence of Pseudomonas syringae in light‐exposed Arabidopsis leaves
dc.creator.none.fl_str_mv Moriconi, Victoria
Sellaro, Romina Vanesa
Ayub, Nicolás Daniel
Soto, Gabriela
Rugnone, Matias Leandro
Shah, Rashmi
Pathak, Gopal P.
Gärtner, Wolfgang
Casal, Jorge José
author Moriconi, Victoria
author_facet Moriconi, Victoria
Sellaro, Romina Vanesa
Ayub, Nicolás Daniel
Soto, Gabriela
Rugnone, Matias Leandro
Shah, Rashmi
Pathak, Gopal P.
Gärtner, Wolfgang
Casal, Jorge José
author_role author
author2 Sellaro, Romina Vanesa
Ayub, Nicolás Daniel
Soto, Gabriela
Rugnone, Matias Leandro
Shah, Rashmi
Pathak, Gopal P.
Gärtner, Wolfgang
Casal, Jorge José
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Arabidopsis Thaliana
Pseudomonas syringae
Tomate
Regimenes de Luz
Bioinformática
Tomatoes
Light Regimes
Bioinformatics
topic Arabidopsis Thaliana
Pseudomonas syringae
Tomate
Regimenes de Luz
Bioinformática
Tomatoes
Light Regimes
Bioinformatics
dc.description.none.fl_txt_mv In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component system (Pst–Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) modulates virulence against A. thaliana. Bioinformatic analysis and the existence of an episomal circular intermediate indicate that the locus encoding Pst–Lov is present in an active genomic island acquired by horizontal transfer. Strains mutated at Pst–Lov showed enhanced growth on minimal medium and in leaves of A. thaliana exposed to light, but not in leaves incubated in darkness or buried in the soil. Pst–Lov repressed the expression of principal and alternative sigma factor genes and their downstream targets linked to bacterial growth, virulence and quorum sensing, in a strictly light‐dependent manner. We propose that the function of Pst–Lov is to distinguish between soil (dark) and leaf (light) environments, attenuating the damage caused to host tissues while releasing growth out of the host. Therefore, in addition to its direct actions via photosynthesis and plant sensory receptors, light may affect plants indirectly via the sensory receptors of bacterial pathogens.
Instituto de Genética
Fil: Moriconi, Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina
Fil: Sellaro, Romina Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina.
Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina.
Fil: Rugnone, Matias Leandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina
Fil: Shah, Rashmi. Max Planck Institute for Chemical Energy Conversion; Alemania
Fil: Pathak, Gopal P.. Max Planck Institute for Chemical Energy Conversion; Alemania
Fil: Gärtner, Wolfgang. Max Planck Institute for Chemical Energy Conversion; Alemania
Fil: Casal, Jorge José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas; Argentina
description In Arabidopsis thaliana, light signals modulate the defences against bacteria. Here we show that light perceived by the LOV domain‐regulated two‐component system (Pst–Lov) of Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) modulates virulence against A. thaliana. Bioinformatic analysis and the existence of an episomal circular intermediate indicate that the locus encoding Pst–Lov is present in an active genomic island acquired by horizontal transfer. Strains mutated at Pst–Lov showed enhanced growth on minimal medium and in leaves of A. thaliana exposed to light, but not in leaves incubated in darkness or buried in the soil. Pst–Lov repressed the expression of principal and alternative sigma factor genes and their downstream targets linked to bacterial growth, virulence and quorum sensing, in a strictly light‐dependent manner. We propose that the function of Pst–Lov is to distinguish between soil (dark) and leaf (light) environments, attenuating the damage caused to host tissues while releasing growth out of the host. Therefore, in addition to its direct actions via photosynthesis and plant sensory receptors, light may affect plants indirectly via the sensory receptors of bacterial pathogens.
publishDate 2013
dc.date.none.fl_str_mv 2013-10
2019-05-16T12:51:23Z
2019-05-16T12:51:23Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://onlinelibrary.wiley.com/doi/10.1111/tpj.12289/abstract
http://hdl.handle.net/20.500.12123/5128
0960-7412
1365-313X
https://doi.org/10.1111/tpj.12289
url http://onlinelibrary.wiley.com/doi/10.1111/tpj.12289/abstract
http://hdl.handle.net/20.500.12123/5128
https://doi.org/10.1111/tpj.12289
identifier_str_mv 0960-7412
1365-313X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Plant Journal 76 (2) : 322-331 (October 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341366253748224
score 12.623145

Publicaciones similares