Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells

Autores
Ramhorst, Rosanna Elizabeth; Fraccaroli, Laura Virginia; Aldo, Paulomi; Alvero, Ayesha B.; Cardenas, Ingrid; Perez Leiros, Claudia; Mor, Gil
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Problem The specialized regulatory T-cells (Treg) population, essential for maternal tolerance of the fetus, performs its suppressive actions in the critical peri-implantation phase of pregnancy. In the present work, we investigated whether trophoblast cells are able to induce Treg recruitment, differentiation, and whether these mechanisms are modified by a bacterial or viral infection. Method of Study Human T-regulatory cells were differentiated from naïve CD45RA + CCR7 + cells obtained from peripheral blood mononuclear cells cultured with IL-2 and TGFβ over 5days. Induction of iTregs (CD4 +Foxp3 + cells) was evaluated using low serum conditioned media (LSCM), obtained from two first trimester trophoblast cell lines, Swan-71 and HTR8. Coculture experiments were carried out using transwell assays where trophoblast cells were in the absence or presence of PGN, LPS, or Poly [I:C]. Cytokine production was measured by multiplex analysis. Results Trophoblast cells constitutively secrete high levels of TGFβ and induced a significant increase of Foxp3 expression accompanied by a specific T-reg cytokine profile. Moreover, trophoblast cells were able to recruit iTregs in a specific manner. Conclusion We demonstrate that trophoblast cells have an active role on the recruitment and differentiation of iTregs, therefore, contributing to the process of immune regulation at the placental-maternal interface. © 2011 John Wiley & Sons A/S.
Fil: Ramhorst, Rosanna Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; Argentina
Fil: Fraccaroli, Laura Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; Argentina
Fil: Aldo, Paulomi. University of Yale; Estados Unidos
Fil: Alvero, Ayesha B.. University of Yale; Estados Unidos
Fil: Cardenas, Ingrid. University of Yale; Estados Unidos
Fil: Perez Leiros, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; Argentina
Fil: Mor, Gil. University of Yale; Estados Unidos
Materia
Early Pregnancy
Human Implantation
Regulatory T Cells
Tolerance And Pregnancy
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/66667
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/66667
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cellsRamhorst, Rosanna ElizabethFraccaroli, Laura VirginiaAldo, PaulomiAlvero, Ayesha B.Cardenas, IngridPerez Leiros, ClaudiaMor, GilEarly PregnancyHuman ImplantationRegulatory T CellsTolerance And Pregnancyhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Problem The specialized regulatory T-cells (Treg) population, essential for maternal tolerance of the fetus, performs its suppressive actions in the critical peri-implantation phase of pregnancy. In the present work, we investigated whether trophoblast cells are able to induce Treg recruitment, differentiation, and whether these mechanisms are modified by a bacterial or viral infection. Method of Study Human T-regulatory cells were differentiated from naïve CD45RA + CCR7 + cells obtained from peripheral blood mononuclear cells cultured with IL-2 and TGFβ over 5days. Induction of iTregs (CD4 +Foxp3 + cells) was evaluated using low serum conditioned media (LSCM), obtained from two first trimester trophoblast cell lines, Swan-71 and HTR8. Coculture experiments were carried out using transwell assays where trophoblast cells were in the absence or presence of PGN, LPS, or Poly [I:C]. Cytokine production was measured by multiplex analysis. Results Trophoblast cells constitutively secrete high levels of TGFβ and induced a significant increase of Foxp3 expression accompanied by a specific T-reg cytokine profile. Moreover, trophoblast cells were able to recruit iTregs in a specific manner. Conclusion We demonstrate that trophoblast cells have an active role on the recruitment and differentiation of iTregs, therefore, contributing to the process of immune regulation at the placental-maternal interface. © 2011 John Wiley & Sons A/S.Fil: Ramhorst, Rosanna Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; ArgentinaFil: Fraccaroli, Laura Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; ArgentinaFil: Aldo, Paulomi. University of Yale; Estados UnidosFil: Alvero, Ayesha B.. University of Yale; Estados UnidosFil: Cardenas, Ingrid. University of Yale; Estados UnidosFil: Perez Leiros, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; ArgentinaFil: Mor, Gil. University of Yale; Estados UnidosWiley Blackwell Publishing, Inc2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/66667Ramhorst, Rosanna Elizabeth; Fraccaroli, Laura Virginia; Aldo, Paulomi; Alvero, Ayesha B.; Cardenas, Ingrid; et al.; Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells; Wiley Blackwell Publishing, Inc; American Journal of Reproductive Immunology; 67; 1; 1-2012; 17-271046-74088755-8920CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0897.2011.01056.xinfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0897.2011.01056.xinfo:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703637/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:23:44Zoai:ri.conicet.gov.ar:11336/66667instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:23:44.327CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells
title Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells
spellingShingle Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells
Ramhorst, Rosanna Elizabeth
Early Pregnancy
Human Implantation
Regulatory T Cells
Tolerance And Pregnancy
title_short Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells
title_full Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells
title_fullStr Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells
title_full_unstemmed Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells
title_sort Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells
dc.creator.none.fl_str_mv Ramhorst, Rosanna Elizabeth
Fraccaroli, Laura Virginia
Aldo, Paulomi
Alvero, Ayesha B.
Cardenas, Ingrid
Perez Leiros, Claudia
Mor, Gil
author Ramhorst, Rosanna Elizabeth
author_facet Ramhorst, Rosanna Elizabeth
Fraccaroli, Laura Virginia
Aldo, Paulomi
Alvero, Ayesha B.
Cardenas, Ingrid
Perez Leiros, Claudia
Mor, Gil
author_role author
author2 Fraccaroli, Laura Virginia
Aldo, Paulomi
Alvero, Ayesha B.
Cardenas, Ingrid
Perez Leiros, Claudia
Mor, Gil
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Early Pregnancy
Human Implantation
Regulatory T Cells
Tolerance And Pregnancy
topic Early Pregnancy
Human Implantation
Regulatory T Cells
Tolerance And Pregnancy
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Problem The specialized regulatory T-cells (Treg) population, essential for maternal tolerance of the fetus, performs its suppressive actions in the critical peri-implantation phase of pregnancy. In the present work, we investigated whether trophoblast cells are able to induce Treg recruitment, differentiation, and whether these mechanisms are modified by a bacterial or viral infection. Method of Study Human T-regulatory cells were differentiated from naïve CD45RA + CCR7 + cells obtained from peripheral blood mononuclear cells cultured with IL-2 and TGFβ over 5days. Induction of iTregs (CD4 +Foxp3 + cells) was evaluated using low serum conditioned media (LSCM), obtained from two first trimester trophoblast cell lines, Swan-71 and HTR8. Coculture experiments were carried out using transwell assays where trophoblast cells were in the absence or presence of PGN, LPS, or Poly [I:C]. Cytokine production was measured by multiplex analysis. Results Trophoblast cells constitutively secrete high levels of TGFβ and induced a significant increase of Foxp3 expression accompanied by a specific T-reg cytokine profile. Moreover, trophoblast cells were able to recruit iTregs in a specific manner. Conclusion We demonstrate that trophoblast cells have an active role on the recruitment and differentiation of iTregs, therefore, contributing to the process of immune regulation at the placental-maternal interface. © 2011 John Wiley & Sons A/S.
Fil: Ramhorst, Rosanna Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; Argentina
Fil: Fraccaroli, Laura Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; Argentina
Fil: Aldo, Paulomi. University of Yale; Estados Unidos
Fil: Alvero, Ayesha B.. University of Yale; Estados Unidos
Fil: Cardenas, Ingrid. University of Yale; Estados Unidos
Fil: Perez Leiros, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Inmunofarmacología; Argentina
Fil: Mor, Gil. University of Yale; Estados Unidos
description Problem The specialized regulatory T-cells (Treg) population, essential for maternal tolerance of the fetus, performs its suppressive actions in the critical peri-implantation phase of pregnancy. In the present work, we investigated whether trophoblast cells are able to induce Treg recruitment, differentiation, and whether these mechanisms are modified by a bacterial or viral infection. Method of Study Human T-regulatory cells were differentiated from naïve CD45RA + CCR7 + cells obtained from peripheral blood mononuclear cells cultured with IL-2 and TGFβ over 5days. Induction of iTregs (CD4 +Foxp3 + cells) was evaluated using low serum conditioned media (LSCM), obtained from two first trimester trophoblast cell lines, Swan-71 and HTR8. Coculture experiments were carried out using transwell assays where trophoblast cells were in the absence or presence of PGN, LPS, or Poly [I:C]. Cytokine production was measured by multiplex analysis. Results Trophoblast cells constitutively secrete high levels of TGFβ and induced a significant increase of Foxp3 expression accompanied by a specific T-reg cytokine profile. Moreover, trophoblast cells were able to recruit iTregs in a specific manner. Conclusion We demonstrate that trophoblast cells have an active role on the recruitment and differentiation of iTregs, therefore, contributing to the process of immune regulation at the placental-maternal interface. © 2011 John Wiley & Sons A/S.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/66667
Ramhorst, Rosanna Elizabeth; Fraccaroli, Laura Virginia; Aldo, Paulomi; Alvero, Ayesha B.; Cardenas, Ingrid; et al.; Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells; Wiley Blackwell Publishing, Inc; American Journal of Reproductive Immunology; 67; 1; 1-2012; 17-27
1046-7408
8755-8920
CONICET Digital
CONICET
url http://hdl.handle.net/11336/66667
identifier_str_mv Ramhorst, Rosanna Elizabeth; Fraccaroli, Laura Virginia; Aldo, Paulomi; Alvero, Ayesha B.; Cardenas, Ingrid; et al.; Modulation and recruitment of inducible regulatory T cells by first trimester trophoblast cells; Wiley Blackwell Publishing, Inc; American Journal of Reproductive Immunology; 67; 1; 1-2012; 17-27
1046-7408
8755-8920
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1600-0897.2011.01056.x
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1600-0897.2011.01056.x
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3703637/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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