Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
- Autores
- Russo, Romina M.; Pietronave, Hernán Pablo; Conte, Claudia Alejandra; Liendo, María Clara; Basilio, Alicia; Lanzavecchia, Silvia Beatriz; Scannapieco, Alejandra Carla
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Grooming behavior is one of the mechanisms of social immunity in Apis mellifera. This behavior has been proposed as an active strategy of honey bees to restrain the population growth of the ectoparasitic mite Varroa destructor in honey bee colonies. The characterization of honey bee stocks with high grooming behavior is of utmost importance for honey bee breeding programs to set the background for mite resistance biomarker-based selection. In this study, we analyzed the expression level of 11 candidate genes putatively involved in grooming and hygiene behaviors in adult workers from mite-resistant (R) and mite-susceptible (S) honey bee stocks. Heads and bodies of worker bees from both stocks, previously tested for grooming response to two treatments (mite infestation and a paintbrush touch control stimulus) were assessed by qPCR. In the head, R bees exposed to mite infestation showed higher levels of Nrx1 and Dop2 and lower levels of Obp3 than S bees. At the body level, R and S bees differed in the expression levels of Nrx1, Oa1, Obp4, Obp14, Obp16, Obp18, Spf45, CYP9Q3, with no stimulus-specific pattern. Overall, our results suggest the involvement of some of the analyzed genes in the specific response to mite infestation, possibly related to the sensitivity and specificity of the R bee to this stimulus at the head level, while other genes would be involved in the nonspecific motor response to irritants at the body level. The present study provides new insights into the characterization of the grooming behavior in a selected honey bee stock and increases the available information on its underlying molecular mechanisms. We discuss the putative functions and use of the assessed genes as potential tools for biomarker-assisted selection and improvement of Varroa mite control strategies in honey bee colonies.
Fil: Russo, Romina M.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Pietronave, Hernán Pablo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Reconquista; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Basilio, Alicia. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Avicultura, Cunicultura y Apicultura; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina - Materia
-
Apis mellifera
Varroa-resistance
grooming, gene expression
honey bee stocks
Varroa destructor - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/264513
Ver los metadatos del registro completo
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Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey beesRusso, Romina M.Pietronave, Hernán PabloConte, Claudia AlejandraLiendo, María ClaraBasilio, AliciaLanzavecchia, Silvia BeatrizScannapieco, Alejandra CarlaApis melliferaVarroa-resistancegrooming, gene expressionhoney bee stocksVarroa destructorhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Grooming behavior is one of the mechanisms of social immunity in Apis mellifera. This behavior has been proposed as an active strategy of honey bees to restrain the population growth of the ectoparasitic mite Varroa destructor in honey bee colonies. The characterization of honey bee stocks with high grooming behavior is of utmost importance for honey bee breeding programs to set the background for mite resistance biomarker-based selection. In this study, we analyzed the expression level of 11 candidate genes putatively involved in grooming and hygiene behaviors in adult workers from mite-resistant (R) and mite-susceptible (S) honey bee stocks. Heads and bodies of worker bees from both stocks, previously tested for grooming response to two treatments (mite infestation and a paintbrush touch control stimulus) were assessed by qPCR. In the head, R bees exposed to mite infestation showed higher levels of Nrx1 and Dop2 and lower levels of Obp3 than S bees. At the body level, R and S bees differed in the expression levels of Nrx1, Oa1, Obp4, Obp14, Obp16, Obp18, Spf45, CYP9Q3, with no stimulus-specific pattern. Overall, our results suggest the involvement of some of the analyzed genes in the specific response to mite infestation, possibly related to the sensitivity and specificity of the R bee to this stimulus at the head level, while other genes would be involved in the nonspecific motor response to irritants at the body level. The present study provides new insights into the characterization of the grooming behavior in a selected honey bee stock and increases the available information on its underlying molecular mechanisms. We discuss the putative functions and use of the assessed genes as potential tools for biomarker-assisted selection and improvement of Varroa mite control strategies in honey bee colonies.Fil: Russo, Romina M.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Pietronave, Hernán Pablo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Reconquista; ArgentinaFil: Conte, Claudia Alejandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Liendo, María Clara. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Basilio, Alicia. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Avicultura, Cunicultura y Apicultura; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; ArgentinaFrontiers Media2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/264513Russo, Romina M.; Pietronave, Hernán Pablo; Conte, Claudia Alejandra; Liendo, María Clara; Basilio, Alicia; et al.; Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees; Frontiers Media; Frontiers in Bee Science; 2; 7-2024; 1-122813-5911CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/frbee.2024.1441317/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/frbee.2024.1441317info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:27Zoai:ri.conicet.gov.ar:11336/264513instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:27.796CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees |
title |
Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees |
spellingShingle |
Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees Russo, Romina M. Apis mellifera Varroa-resistance grooming, gene expression honey bee stocks Varroa destructor |
title_short |
Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees |
title_full |
Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees |
title_fullStr |
Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees |
title_full_unstemmed |
Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees |
title_sort |
Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees |
dc.creator.none.fl_str_mv |
Russo, Romina M. Pietronave, Hernán Pablo Conte, Claudia Alejandra Liendo, María Clara Basilio, Alicia Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
author |
Russo, Romina M. |
author_facet |
Russo, Romina M. Pietronave, Hernán Pablo Conte, Claudia Alejandra Liendo, María Clara Basilio, Alicia Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
author_role |
author |
author2 |
Pietronave, Hernán Pablo Conte, Claudia Alejandra Liendo, María Clara Basilio, Alicia Lanzavecchia, Silvia Beatriz Scannapieco, Alejandra Carla |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Apis mellifera Varroa-resistance grooming, gene expression honey bee stocks Varroa destructor |
topic |
Apis mellifera Varroa-resistance grooming, gene expression honey bee stocks Varroa destructor |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Grooming behavior is one of the mechanisms of social immunity in Apis mellifera. This behavior has been proposed as an active strategy of honey bees to restrain the population growth of the ectoparasitic mite Varroa destructor in honey bee colonies. The characterization of honey bee stocks with high grooming behavior is of utmost importance for honey bee breeding programs to set the background for mite resistance biomarker-based selection. In this study, we analyzed the expression level of 11 candidate genes putatively involved in grooming and hygiene behaviors in adult workers from mite-resistant (R) and mite-susceptible (S) honey bee stocks. Heads and bodies of worker bees from both stocks, previously tested for grooming response to two treatments (mite infestation and a paintbrush touch control stimulus) were assessed by qPCR. In the head, R bees exposed to mite infestation showed higher levels of Nrx1 and Dop2 and lower levels of Obp3 than S bees. At the body level, R and S bees differed in the expression levels of Nrx1, Oa1, Obp4, Obp14, Obp16, Obp18, Spf45, CYP9Q3, with no stimulus-specific pattern. Overall, our results suggest the involvement of some of the analyzed genes in the specific response to mite infestation, possibly related to the sensitivity and specificity of the R bee to this stimulus at the head level, while other genes would be involved in the nonspecific motor response to irritants at the body level. The present study provides new insights into the characterization of the grooming behavior in a selected honey bee stock and increases the available information on its underlying molecular mechanisms. We discuss the putative functions and use of the assessed genes as potential tools for biomarker-assisted selection and improvement of Varroa mite control strategies in honey bee colonies. Fil: Russo, Romina M.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Pietronave, Hernán Pablo. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Reconquista; Argentina Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Liendo, María Clara. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Basilio, Alicia. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Avicultura, Cunicultura y Apicultura; Argentina Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigacion En Ciencias Veterinarias y Agronomicas. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Oficina de Coordinacion Administrativa Pque. Centenario. Instituto de Agrobiotecnologia y Biologia Molecular. Grupo Vinculado Instituto de Genetica "ewald A. Favret" Al Iabimo.; Argentina |
description |
Grooming behavior is one of the mechanisms of social immunity in Apis mellifera. This behavior has been proposed as an active strategy of honey bees to restrain the population growth of the ectoparasitic mite Varroa destructor in honey bee colonies. The characterization of honey bee stocks with high grooming behavior is of utmost importance for honey bee breeding programs to set the background for mite resistance biomarker-based selection. In this study, we analyzed the expression level of 11 candidate genes putatively involved in grooming and hygiene behaviors in adult workers from mite-resistant (R) and mite-susceptible (S) honey bee stocks. Heads and bodies of worker bees from both stocks, previously tested for grooming response to two treatments (mite infestation and a paintbrush touch control stimulus) were assessed by qPCR. In the head, R bees exposed to mite infestation showed higher levels of Nrx1 and Dop2 and lower levels of Obp3 than S bees. At the body level, R and S bees differed in the expression levels of Nrx1, Oa1, Obp4, Obp14, Obp16, Obp18, Spf45, CYP9Q3, with no stimulus-specific pattern. Overall, our results suggest the involvement of some of the analyzed genes in the specific response to mite infestation, possibly related to the sensitivity and specificity of the R bee to this stimulus at the head level, while other genes would be involved in the nonspecific motor response to irritants at the body level. The present study provides new insights into the characterization of the grooming behavior in a selected honey bee stock and increases the available information on its underlying molecular mechanisms. We discuss the putative functions and use of the assessed genes as potential tools for biomarker-assisted selection and improvement of Varroa mite control strategies in honey bee colonies. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/264513 Russo, Romina M.; Pietronave, Hernán Pablo; Conte, Claudia Alejandra; Liendo, María Clara; Basilio, Alicia; et al.; Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees; Frontiers Media; Frontiers in Bee Science; 2; 7-2024; 1-12 2813-5911 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/264513 |
identifier_str_mv |
Russo, Romina M.; Pietronave, Hernán Pablo; Conte, Claudia Alejandra; Liendo, María Clara; Basilio, Alicia; et al.; Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees; Frontiers Media; Frontiers in Bee Science; 2; 7-2024; 1-12 2813-5911 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/frbee.2024.1441317/full info:eu-repo/semantics/altIdentifier/doi/10.3389/frbee.2024.1441317 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Frontiers Media |
publisher.none.fl_str_mv |
Frontiers Media |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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13.070432 |