Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees

Autores
Russo, Romina Maria; Pietronave, Hernan Pablo; Conte, Claudia Alejandra; Liendo, María Clara; Basilio, Alicia Mabel; Lanzavecchia, Silvia Beatriz; Scannapieco, Alejandra Carla
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Grooming behavior is one of the mechanisms of social immunity in Apis mellifera. This behavior has been proposed as an active strategy of honey bees to restrain the population growth of the ectoparasitic mite Varroa destructor in honey bee colonies. The characterization of honey bee stocks with high grooming behavior is of utmost importance for honey bee breeding programs to set the background for mite resistance biomarker-based selection. In this study, we analyzed the expression level of 11 candidate genes putatively involved in grooming and hygiene behaviors in adult workers from mite-resistant (R) and mite-susceptible (S) honey bee stocks. Heads and bodies of worker bees from both stocks, previously tested for grooming response to two treatments (mite infestation and a paintbrush touch control stimulus) were assessed by qPCR. In the head, R bees exposed to mite infestation showed higher levels of Nrx1 and Dop2 and lower levels of Obp3 than S bees. At the body level, R and S bees differed in the expression levels of Nrx1, Oa1, Obp4, Obp14, Obp16, Obp18, Spf45, CYP9Q3, with no stimulus-specific pattern. Overall, our results suggest the involvement of some of the analyzed genes in the specific response to mite infestation, possibly related to the sensitivity and specificity of the R bee to this stimulus at the head level, while other genes would be involved in the non-specific motor response to irritants at the body level. The present study provides new insights into the characterization of the grooming behavior in a selected honey bee stock and increases the available information on its underlying molecular mechanisms. We discuss the putative functions and use of the assessed genes as potential tools for biomarker-assisted selection and improvement of Varroa mite control strategies in honey bee colonies.
Instituto de Genética
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pietronave, Hernán Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Conte, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); Argentina
Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Basilio, Alicia Mabel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Avicultura, Cunicultura y Apicultura; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Frontiers in Bee Science 2 : 1441317 (July 2024)
Materia
Apis mellifera
Varroa destructor
Gene Expression
Honey Bees
Disease Resistance
Expresión Génica
Abeja Melífera
Resistencia a la Enfermedad
Grooming Behavior
Comportamiento de Aseo
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22048
Acceder
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oai_identifier_str oai:localhost:20.500.12123/22048
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spelling Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey beesRusso, Romina MariaPietronave, Hernan PabloConte, Claudia AlejandraLiendo, María ClaraBasilio, Alicia MabelLanzavecchia, Silvia BeatrizScannapieco, Alejandra CarlaApis melliferaVarroa destructorGene ExpressionHoney BeesDisease ResistanceExpresión GénicaAbeja MelíferaResistencia a la EnfermedadGrooming BehaviorComportamiento de AseoGrooming behavior is one of the mechanisms of social immunity in Apis mellifera. This behavior has been proposed as an active strategy of honey bees to restrain the population growth of the ectoparasitic mite Varroa destructor in honey bee colonies. The characterization of honey bee stocks with high grooming behavior is of utmost importance for honey bee breeding programs to set the background for mite resistance biomarker-based selection. In this study, we analyzed the expression level of 11 candidate genes putatively involved in grooming and hygiene behaviors in adult workers from mite-resistant (R) and mite-susceptible (S) honey bee stocks. Heads and bodies of worker bees from both stocks, previously tested for grooming response to two treatments (mite infestation and a paintbrush touch control stimulus) were assessed by qPCR. In the head, R bees exposed to mite infestation showed higher levels of Nrx1 and Dop2 and lower levels of Obp3 than S bees. At the body level, R and S bees differed in the expression levels of Nrx1, Oa1, Obp4, Obp14, Obp16, Obp18, Spf45, CYP9Q3, with no stimulus-specific pattern. Overall, our results suggest the involvement of some of the analyzed genes in the specific response to mite infestation, possibly related to the sensitivity and specificity of the R bee to this stimulus at the head level, while other genes would be involved in the non-specific motor response to irritants at the body level. The present study provides new insights into the characterization of the grooming behavior in a selected honey bee stock and increases the available information on its underlying molecular mechanisms. We discuss the putative functions and use of the assessed genes as potential tools for biomarker-assisted selection and improvement of Varroa mite control strategies in honey bee colonies.Instituto de GenéticaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pietronave, Hernán Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; ArgentinaFil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; ArgentinaFil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Conte, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); ArgentinaFil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Basilio, Alicia Mabel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Avicultura, Cunicultura y Apicultura; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Media2025-04-24T16:03:00Z2025-04-24T16:03:00Z2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22048https://www.frontiersin.org/journals/bee-science/articles/10.3389/frbee.2024.1441317/full2813-5911https://doi.org/10.3389/frbee.2024.1441317Frontiers in Bee Science 2 : 1441317 (July 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PE-L01-I069, Aportes al desarrollo sostenible de la apicultura argentinainfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:15Zoai:localhost:20.500.12123/22048instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:15.441INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
title Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
spellingShingle Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
Russo, Romina Maria
Apis mellifera
Varroa destructor
Gene Expression
Honey Bees
Disease Resistance
Expresión Génica
Abeja Melífera
Resistencia a la Enfermedad
Grooming Behavior
Comportamiento de Aseo
title_short Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
title_full Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
title_fullStr Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
title_full_unstemmed Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
title_sort Stimulus-specific gene expression profiles associated with grooming behavior and Varroa destructor resistance in honey bees
dc.creator.none.fl_str_mv Russo, Romina Maria
Pietronave, Hernan Pablo
Conte, Claudia Alejandra
Liendo, María Clara
Basilio, Alicia Mabel
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author Russo, Romina Maria
author_facet Russo, Romina Maria
Pietronave, Hernan Pablo
Conte, Claudia Alejandra
Liendo, María Clara
Basilio, Alicia Mabel
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author_role author
author2 Pietronave, Hernan Pablo
Conte, Claudia Alejandra
Liendo, María Clara
Basilio, Alicia Mabel
Lanzavecchia, Silvia Beatriz
Scannapieco, Alejandra Carla
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Apis mellifera
Varroa destructor
Gene Expression
Honey Bees
Disease Resistance
Expresión Génica
Abeja Melífera
Resistencia a la Enfermedad
Grooming Behavior
Comportamiento de Aseo
topic Apis mellifera
Varroa destructor
Gene Expression
Honey Bees
Disease Resistance
Expresión Génica
Abeja Melífera
Resistencia a la Enfermedad
Grooming Behavior
Comportamiento de Aseo
dc.description.none.fl_txt_mv Grooming behavior is one of the mechanisms of social immunity in Apis mellifera. This behavior has been proposed as an active strategy of honey bees to restrain the population growth of the ectoparasitic mite Varroa destructor in honey bee colonies. The characterization of honey bee stocks with high grooming behavior is of utmost importance for honey bee breeding programs to set the background for mite resistance biomarker-based selection. In this study, we analyzed the expression level of 11 candidate genes putatively involved in grooming and hygiene behaviors in adult workers from mite-resistant (R) and mite-susceptible (S) honey bee stocks. Heads and bodies of worker bees from both stocks, previously tested for grooming response to two treatments (mite infestation and a paintbrush touch control stimulus) were assessed by qPCR. In the head, R bees exposed to mite infestation showed higher levels of Nrx1 and Dop2 and lower levels of Obp3 than S bees. At the body level, R and S bees differed in the expression levels of Nrx1, Oa1, Obp4, Obp14, Obp16, Obp18, Spf45, CYP9Q3, with no stimulus-specific pattern. Overall, our results suggest the involvement of some of the analyzed genes in the specific response to mite infestation, possibly related to the sensitivity and specificity of the R bee to this stimulus at the head level, while other genes would be involved in the non-specific motor response to irritants at the body level. The present study provides new insights into the characterization of the grooming behavior in a selected honey bee stock and increases the available information on its underlying molecular mechanisms. We discuss the putative functions and use of the assessed genes as potential tools for biomarker-assisted selection and improvement of Varroa mite control strategies in honey bee colonies.
Instituto de Genética
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Russo, Romina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Russo, Romina Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pietronave, Hernán Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Reconquista; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Conte, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Liendo, María Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular(IABIMO); Argentina
Fil: Liendo, María Clara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Basilio, Alicia Mabel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Animal. Cátedra de Avicultura, Cunicultura y Apicultura; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Scannapieco, Alejandra Carla. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Scannapieco, Alejandra Carla. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Grooming behavior is one of the mechanisms of social immunity in Apis mellifera. This behavior has been proposed as an active strategy of honey bees to restrain the population growth of the ectoparasitic mite Varroa destructor in honey bee colonies. The characterization of honey bee stocks with high grooming behavior is of utmost importance for honey bee breeding programs to set the background for mite resistance biomarker-based selection. In this study, we analyzed the expression level of 11 candidate genes putatively involved in grooming and hygiene behaviors in adult workers from mite-resistant (R) and mite-susceptible (S) honey bee stocks. Heads and bodies of worker bees from both stocks, previously tested for grooming response to two treatments (mite infestation and a paintbrush touch control stimulus) were assessed by qPCR. In the head, R bees exposed to mite infestation showed higher levels of Nrx1 and Dop2 and lower levels of Obp3 than S bees. At the body level, R and S bees differed in the expression levels of Nrx1, Oa1, Obp4, Obp14, Obp16, Obp18, Spf45, CYP9Q3, with no stimulus-specific pattern. Overall, our results suggest the involvement of some of the analyzed genes in the specific response to mite infestation, possibly related to the sensitivity and specificity of the R bee to this stimulus at the head level, while other genes would be involved in the non-specific motor response to irritants at the body level. The present study provides new insights into the characterization of the grooming behavior in a selected honey bee stock and increases the available information on its underlying molecular mechanisms. We discuss the putative functions and use of the assessed genes as potential tools for biomarker-assisted selection and improvement of Varroa mite control strategies in honey bee colonies.
publishDate 2024
dc.date.none.fl_str_mv 2024-07
2025-04-24T16:03:00Z
2025-04-24T16:03:00Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22048
https://www.frontiersin.org/journals/bee-science/articles/10.3389/frbee.2024.1441317/full
2813-5911
https://doi.org/10.3389/frbee.2024.1441317
url http://hdl.handle.net/20.500.12123/22048
https://www.frontiersin.org/journals/bee-science/articles/10.3389/frbee.2024.1441317/full
https://doi.org/10.3389/frbee.2024.1441317
identifier_str_mv 2813-5911
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PE-L01-I069, Aportes al desarrollo sostenible de la apicultura argentina
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Bee Science 2 : 1441317 (July 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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