Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.

Autores
Molinari, Maria Laura; Boiardi, Jose Luis
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Growth of Gluconacetobacter diazotrophicus with glucose as carbon an energy source has been extensively studied. But there are no reports in the literature describing growth of Gluconacetobacter diazotrophicus in batch culture containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. We focused in biomass, levans, gluconic acid and keto gluconic acids productions under different sucrose concentration and type nitrogen source. Results: The biomass production was maximal in culture containing 100 g/l sucrose and inorganic nitrogen. The gluconic acid was production in all condition, detecting up to 9 g/l in cultures with sucrose excess and biological N2-fixation. Keto gluconic acids were detectable only in cultures in sucrose excess and enriched with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 g/l sucrose, BNF or organic nitrogen source with value was up to 25 g/l. It cultures were observed a significant LsdA expression. Conclusions: Ours results, not only describe some unknown aspect of G. diazotrophicus physiology, open the possibility of developed a technology of biomass, levans and gluconic acid production by G. diazotrophicus using sucrose as C-sources or an enzymatic extract. These products are interest biotechnology because they have a commercial important in different industrial process.
Fil: Molinari, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Boiardi, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Materia
growth
biomass
levans
gluconic acid
keto gluconic acid.
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/3388
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/3388
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.Molinari, Maria LauraBoiardi, Jose Luisgrowthbiomasslevansgluconic acidketo gluconic acid.https://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Background: Growth of Gluconacetobacter diazotrophicus with glucose as carbon an energy source has been extensively studied. But there are no reports in the literature describing growth of Gluconacetobacter diazotrophicus in batch culture containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. We focused in biomass, levans, gluconic acid and keto gluconic acids productions under different sucrose concentration and type nitrogen source. Results: The biomass production was maximal in culture containing 100 g/l sucrose and inorganic nitrogen. The gluconic acid was production in all condition, detecting up to 9 g/l in cultures with sucrose excess and biological N2-fixation. Keto gluconic acids were detectable only in cultures in sucrose excess and enriched with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 g/l sucrose, BNF or organic nitrogen source with value was up to 25 g/l. It cultures were observed a significant LsdA expression. Conclusions: Ours results, not only describe some unknown aspect of G. diazotrophicus physiology, open the possibility of developed a technology of biomass, levans and gluconic acid production by G. diazotrophicus using sucrose as C-sources or an enzymatic extract. These products are interest biotechnology because they have a commercial important in different industrial process.Fil: Molinari, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaFil: Boiardi, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); ArgentinaPontificia Universidad Catolica de Valparaíso2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/3388Molinari, Maria Laura; Boiardi, Jose Luis; Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.; Pontificia Universidad Catolica de Valparaíso; Electronic Journal Of Biotechnology; 16; 9; 1-2013; 1-70717-3458enginfo:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9xprsqinfo:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.infoinfo:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:54Zoai:ri.conicet.gov.ar:11336/3388instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:54.367CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.
title Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.
spellingShingle Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.
Molinari, Maria Laura
growth
biomass
levans
gluconic acid
keto gluconic acid.
title_short Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.
title_full Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.
title_fullStr Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.
title_full_unstemmed Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.
title_sort Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.
dc.creator.none.fl_str_mv Molinari, Maria Laura
Boiardi, Jose Luis
author Molinari, Maria Laura
author_facet Molinari, Maria Laura
Boiardi, Jose Luis
author_role author
author2 Boiardi, Jose Luis
author2_role author
dc.subject.none.fl_str_mv growth
biomass
levans
gluconic acid
keto gluconic acid.
topic growth
biomass
levans
gluconic acid
keto gluconic acid.
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Background: Growth of Gluconacetobacter diazotrophicus with glucose as carbon an energy source has been extensively studied. But there are no reports in the literature describing growth of Gluconacetobacter diazotrophicus in batch culture containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. We focused in biomass, levans, gluconic acid and keto gluconic acids productions under different sucrose concentration and type nitrogen source. Results: The biomass production was maximal in culture containing 100 g/l sucrose and inorganic nitrogen. The gluconic acid was production in all condition, detecting up to 9 g/l in cultures with sucrose excess and biological N2-fixation. Keto gluconic acids were detectable only in cultures in sucrose excess and enriched with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 g/l sucrose, BNF or organic nitrogen source with value was up to 25 g/l. It cultures were observed a significant LsdA expression. Conclusions: Ours results, not only describe some unknown aspect of G. diazotrophicus physiology, open the possibility of developed a technology of biomass, levans and gluconic acid production by G. diazotrophicus using sucrose as C-sources or an enzymatic extract. These products are interest biotechnology because they have a commercial important in different industrial process.
Fil: Molinari, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
Fil: Boiardi, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales (i); Argentina
description Background: Growth of Gluconacetobacter diazotrophicus with glucose as carbon an energy source has been extensively studied. But there are no reports in the literature describing growth of Gluconacetobacter diazotrophicus in batch culture containing sucrose as carbon source. The first step in sucrose pathway and production of levans was investigated. We focused in biomass, levans, gluconic acid and keto gluconic acids productions under different sucrose concentration and type nitrogen source. Results: The biomass production was maximal in culture containing 100 g/l sucrose and inorganic nitrogen. The gluconic acid was production in all condition, detecting up to 9 g/l in cultures with sucrose excess and biological N2-fixation. Keto gluconic acids were detectable only in cultures in sucrose excess and enriched with organic nitrogen sources. Levans production, although observed in all cultures, was maximal in batch culture with 100 g/l sucrose, BNF or organic nitrogen source with value was up to 25 g/l. It cultures were observed a significant LsdA expression. Conclusions: Ours results, not only describe some unknown aspect of G. diazotrophicus physiology, open the possibility of developed a technology of biomass, levans and gluconic acid production by G. diazotrophicus using sucrose as C-sources or an enzymatic extract. These products are interest biotechnology because they have a commercial important in different industrial process.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/3388
Molinari, Maria Laura; Boiardi, Jose Luis; Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.; Pontificia Universidad Catolica de Valparaíso; Electronic Journal Of Biotechnology; 16; 9; 1-2013; 1-7
0717-3458
url http://hdl.handle.net/11336/3388
identifier_str_mv Molinari, Maria Laura; Boiardi, Jose Luis; Study on batch cultures of gluconacetobacter dizaotrophicus using sucrose.; Pontificia Universidad Catolica de Valparaíso; Electronic Journal Of Biotechnology; 16; 9; 1-2013; 1-7
0717-3458
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/9xprsq
info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pontificia Universidad Catolica de Valparaíso
publisher.none.fl_str_mv Pontificia Universidad Catolica de Valparaíso
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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